An enthusiastic, adaptive and fast-learning person with a broad and acute experience in wide spectrum branches of molecular and cellular biology, virology, immunology, vaccine research, cancer biology, biotechnology and pharmaceutical sciences.
I particularly enjoy collaborating with scientists from various disciplines to develop new skills and solve new challenges in both basic and clinical sciences.
2015 Ph.D., Tehran University of Medical Sciences
Thesis Title: Construction of a dual cassette-based DNA vaccine encoding HCV core antigen and ECL/MIP-3β/CCL19 chemokine as adjuvant and evaluation of immunogenicity in BALB/c mice model.
2006 Pharm.D., Pharmaceutical Sciences Unit
Thesis Title: Utilizing cytokines and immunomodulatory molecules (GM-CSF and IL-23) as immunologic adjuvants to enhance HCV core DNA vaccine immunogenicity in mice model
2014 Management and set up of HIV Viral Load test, Research center for HIV and AIDS(IRCHA).
2009-2014 Research assistant, Tarbiat Modares University
Project title: Construction of HIV-Tat polytope/GM-CSF DNA vaccines and evaluation of the immunogenicity in mice model using dendrosomal nanoparticles as vaccine delivery system.
2009-12 Research assistant, Pasteur Institute
Project title: Cloning and expression of human IFN-lambda1 gene and evaluation of its antiviral properties against HTLV-I
2010-13 Research assistant, Pasteur Institute
Project title: Construction of human Adipose stem cells transduced by recombinant adenoviruses expressing human EGF and TGF-α for regenerative medicine purposes.
2014-15 Instructor (Adjunct faculty), Pharmaceutical Sciences Unit
Immunology (laboratory) course for B.S. degree in Microbiology and Molecular Biology.
DNA cloning, Nucleic acid modification, extraction and purification techniques
DNA Transfection methods
Cell cytotoxicity/Cell proliferation assay
Intracellular signaling Array and assay
Cell migration and invasion assay
Development/ Characterization of Dendritic cells
T cell proliferation assay
ELISA & ELISpot -Immunocytochemistry
Secondary serologic reactions
In vitro virus culture and titration
Recombinant Adenovirus and Lentivirus
Cytopathic effect reduction assay (CPERA)
Viral/ proviral load assay
Termocyclers (PCR,Real time PCR)
Microscope (light field, inverted,fluorescent)
UV/visible plate readers, picodrop detector
Animal model techniques
Handling of laboratory animals (Mice)
DNA vaccine and protein vaccine injection in mice (S.C., I.V. and I.M routes)
Mice bleeding from eye and tail vain
Isolation and dissection of mice spleen, liver and lymph nodes.
Proficient in MS Office (Excel, PowerPoint, and Word).
Bioinformatic software: Gene Runner, CLC, Primer Express,Prism,FlowJo
Statistical Analysis software: SPSS
Construction of recombinant Adenovirus expressing Human Transforming Growth Factor-α (498bp).Patent ID: 80935
Construction of recombinant Adenovirus expressing Human Epidermal Growth Factor (EGF).Patent ID: 84433
Mol Biol Rep. 2014 Sep; 41(9):5943-52.
Modulation of hepatitis C virus core DNA vaccine immune responses by co-immunization with CC-chemokine ligand 20 (CCL20) gene as immunoadjuvant.
Hepat Mon. 2014 Mar 1; 14(3):e14611.
Enhancement of Immune Responses by Co-delivery of CCL19/MIP-3beta Chemokine Plasmid with HCV Core DNA/Protein Immunization.
Cytokine. 2009Apr; 46(1):43-50.
Effect of immunological adjuvants: GM-CSF (granulocyte-monocyte colony stimulating factor) and IL-23 (interleukin-23) on immune responses generated against hepatitis C virus core DNA vaccine.
Cytotherapy. 2014 May; 16(5):612-8.
Evaluation of optimal concentration and exposure duration of valproic acid alone or in combination with ViraDuctin to augment adenovirus transduction in human adipose stem cells.
Immunol Lett. 2011 Oct 30;140(1-2):14-20
ELISPOT analysis of a new CTL based DNA vaccine for HIV-1 using GM-CSF in DNA prime/peptide boost strategy: GM-CSF induced long-lived memory responses.
Acta Virol. 2012; 56(1):11-8.
Evaluation of the in vitro antiretroviral potential of some Biginelli-type pyrimidines.
Arak Medical University Journal (AMUJ) Original Article 2011; 14(57): 69-78
Cloning of human IFN-λ1 (IL-29) from monocyte derived DCs and its expression in HEK 293 T.
Modares Journal of Medical Sciences, Pathobiology Vol.14,No.2, Summer 2011
Designing and construction of bicistronic plasmid pIRES-Igk/m IL-18/Fc: potential implications for vaccine investigations.
YAS Young Investigator Award of 2015 in the 6th Tehran Hepatitis Congress and First Global Hepatitis Meeting (GLOB1).
Awarded by Professor Minoo Mohraz; Head of Research center of HIV and AIDS(IRCHA) for volunteer participation in management and set up of HIV viral load detection in the main laboratory of Infectious Disease center
The Best Oral Presentation of “The First National congress of Clinical Microbiology” 8-10 May 2007, Shiraz. Presentation short-title: HCV DNA vaccine model
Available upon request