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Pharm.D.,Ph.D.

Location:
California, United States
Posted:
December 29, 2015

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Resume:

Christine Hartoonian

Sunland, CA

Tel: 818-***-****

Email:acsx3i@r.postjobfree.com

Professional Profile

An enthusiastic, adaptive and fast-learning person with a broad and acute experience in wide spectrum branches of molecular and cellular biology, virology, immunology, vaccine research, cancer biology, biotechnology and pharmaceutical sciences.

I particularly enjoy collaborating with scientists from various disciplines to develop new skills and solve new challenges in both basic and clinical sciences.

Education

2015 Ph.D., Tehran University of Medical Sciences

Thesis Title: Construction of a dual cassette-based DNA vaccine encoding HCV core antigen and ECL/MIP-3β/CCL19 chemokine as adjuvant and evaluation of immunogenicity in BALB/c mice model.

Evaluation: 20/20

2006 Pharm.D., Pharmaceutical Sciences Unit

Thesis Title: Utilizing cytokines and immunomodulatory molecules (GM-CSF and IL-23) as immunologic adjuvants to enhance HCV core DNA vaccine immunogenicity in mice model

Evaluation: 19.7/20

Professional experience

2014 Management and set up of HIV Viral Load test, Research center for HIV and AIDS(IRCHA).

2009-2014 Research assistant, Tarbiat Modares University

Project title: Construction of HIV-Tat polytope/GM-CSF DNA vaccines and evaluation of the immunogenicity in mice model using dendrosomal nanoparticles as vaccine delivery system.

2009-12 Research assistant, Pasteur Institute

Project title: Cloning and expression of human IFN-lambda1 gene and evaluation of its antiviral properties against HTLV-I

2010-13 Research assistant, Pasteur Institute

Project title: Construction of human Adipose stem cells transduced by recombinant adenoviruses expressing human EGF and TGF-α for regenerative medicine purposes.

2014-15 Instructor (Adjunct faculty), Pharmaceutical Sciences Unit

Immunology (laboratory) course for B.S. degree in Microbiology and Molecular Biology.

Technical skills

Molecular Biology

DNA cloning, Nucleic acid modification, extraction and purification techniques

PCR, qRT-PCR

Cell-based Techniques

DNA Transfection methods

Cell staining

Cell cytotoxicity/Cell proliferation assay

Intracellular signaling Array and assay

Cell migration and invasion assay

Development/ Characterization of Dendritic cells

Immunoassay

Western blot

T cell proliferation assay

ELISA & ELISpot -Immunocytochemistry

Secondary serologic reactions

Virology techniques

In vitro virus culture and titration

Recombinant Adenovirus and Lentivirus

Cytopathic effect reduction assay (CPERA)

Viral/ proviral load assay

Instruments

Termocyclers (PCR,Real time PCR)

Microscope (light field, inverted,fluorescent)

UV/visible plate readers, picodrop detector

Electrophoresis

Flowcytometery

Animal model techniques

Handling of laboratory animals (Mice)

DNA vaccine and protein vaccine injection in mice (S.C., I.V. and I.M routes)

Mice bleeding from eye and tail vain

Isolation and dissection of mice spleen, liver and lymph nodes.

Computer

Proficient in MS Office (Excel, PowerPoint, and Word).

Bioinformatic software: Gene Runner, CLC, Primer Express,Prism,FlowJo

Statistical Analysis software: SPSS

Publications

National Patents:

Construction of recombinant Adenovirus expressing Human Transforming Growth Factor-α (498bp).Patent ID: 80935

Construction of recombinant Adenovirus expressing Human Epidermal Growth Factor (EGF).Patent ID: 84433

Articles

Mol Biol Rep. 2014 Sep; 41(9):5943-52.

Modulation of hepatitis C virus core DNA vaccine immune responses by co-immunization with CC-chemokine ligand 20 (CCL20) gene as immunoadjuvant.

Hepat Mon. 2014 Mar 1; 14(3):e14611.

Enhancement of Immune Responses by Co-delivery of CCL19/MIP-3beta Chemokine Plasmid with HCV Core DNA/Protein Immunization.

Cytokine. 2009Apr; 46(1):43-50.

Effect of immunological adjuvants: GM-CSF (granulocyte-monocyte colony stimulating factor) and IL-23 (interleukin-23) on immune responses generated against hepatitis C virus core DNA vaccine.

Cytotherapy. 2014 May; 16(5):612-8.

Evaluation of optimal concentration and exposure duration of valproic acid alone or in combination with ViraDuctin to augment adenovirus transduction in human adipose stem cells.

Immunol Lett. 2011 Oct 30;140(1-2):14-20

ELISPOT analysis of a new CTL based DNA vaccine for HIV-1 using GM-CSF in DNA prime/peptide boost strategy: GM-CSF induced long-lived memory responses.

Acta Virol. 2012; 56(1):11-8.

Evaluation of the in vitro antiretroviral potential of some Biginelli-type pyrimidines.

Arak Medical University Journal (AMUJ) Original Article 2011; 14(57): 69-78

Cloning of human IFN-λ1 (IL-29) from monocyte derived DCs and its expression in HEK 293 T.

Modares Journal of Medical Sciences, Pathobiology Vol.14,No.2, Summer 2011

Designing and construction of bicistronic plasmid pIRES-Igk/m IL-18/Fc: potential implications for vaccine investigations.

Awards/Honors

YAS Young Investigator Award of 2015 in the 6th Tehran Hepatitis Congress and First Global Hepatitis Meeting (GLOB1).

Awarded by Professor Minoo Mohraz; Head of Research center of HIV and AIDS(IRCHA) for volunteer participation in management and set up of HIV viral load detection in the main laboratory of Infectious Disease center

The Best Oral Presentation of “The First National congress of Clinical Microbiology” 8-10 May 2007, Shiraz. Presentation short-title: HCV DNA vaccine model

Reference

Available upon request



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