Laboratory Technologist

Lynda L. Rennie-Tankersley, MS, MB(ASCP)

410-***-**** • ********@*******.***

Laboratory Technologist

Collaborative, goal-driven, and highly motivated professional with a history of research leadership at The Johns Hopkins University School of Medicine in the areas of cardiology, gastroenterology, and epidemiology coupled with seasoned expertise in molecular and cellular biology. Effective team leader with outstanding communication and problem solving skills. Well rounded background in customer relationship management and laboratory outreach for BD Diagnostics. Strong technical aptitude and troubleshooting acumen with two years as an applications engineer at Brimrose Corp of America. Produced over 20 peer-reviewed journal articles & 10 abstracts. Open to travel.

CITI certified in Human Subjects & Biomedical Research for IRB approval ~ US Citizen

MS in Biotechnology ~ HIV Clinical Trials ~ Independently Plan, Analyze, Interpret & Present Data

Advanced PCR Skills ~ DNA Automated Extraction / Liquid Handling ~ Molecular Biology Expert

Education

MS, Biotechnology, The Johns Hopkins University, Baltimore, Maryland • 1995

BA, Biology, Western Maryland College (McDaniel College), Westminster, Maryland • 1985

Certification

MB (ASCP), American Society for Clinical Pathology, (BOC) Board of Certification • 11.20.2015

Professional Experience

Johns Hopkins University, 98 N. Broadway Ave., Baltimore, MD 21205

• (11/1985 - 8/2006, 11/2014 - 06/2015)

Clinical Laboratory Manager / Sr Laboratory Coordinator, Epidemiology • (11/2014 - 06/2015, 40hrs/wk)

Process clinical samples in CLIA and CAP certified laboratory, document and store with LDMS and FreezerWorks databases for biorepository. Coordinate operations of the automated DNA extraction lab as part of the biological repository servicing center for studies of the natural history of AIDS and other infectious disease-related epidemiological studies. Maintain quality assurance of state and federal regulatory compliance requirements for a certified, clinical lab. Develop and carry out new techniques for different studies in the lab. Supervise and instruct lab staff working in the lab, contribute to writing laboratory reports, protocols, and laboratory standard operating procedures. Maintain lab supplies; oversee lab equipment maintenance including contracts and records of lab equipment. Maintain general lab records; use independent thinking in the conduct of laboratory affairs and for individual research projects related to the general mission of the laboratory. Independently identify and resolve problems involving routine and experimental research assays. Contribute to group meetings, offer suggestions, and actively participate in providing guidance and support to entry-level members. Management of laws and regulations required for human subjects research. Provide technical reviews and oversight for regulating authorities.

•Maintain molecular biology laboratory operation by scheduling experiments, keeping detailed records of daily molecular biology experiments, while maintaining equipment maintenance and repair.

•Execute molecular biological techniques in support of biomedical research projects using approved methods and procedures.

•Modify experiments and procedures applying troubleshooting and problem solving methods in order to guarantee accurate results.

•Consult the literature to ensure that the data is in line with previous results found.

•CLIA and CAP Certified Laboratory experience

•Advanced Automated Robotics

•Certified recently at Hopkins’ Health, Safety and Environment for all laboratories’ required trainings.

•Maintain daily workings of the laboratory by ordering supplies, coordinating research projects with personnel and ensuring functioning order of all equipment.

Laboratory Manager, Cardiology • (11/2005 - 08/2006, 40hrs/wk)

Oversaw operations of a large cardiology laboratory under the leadership of a well-known cardiologist. Presented training to PhD and MD fellows. Facilitated detection of novel genes in the heart through development and implementation of PCR, Real-time PCR, and Taqman chemistry. Conducted isolation of neonatal myocytes. Managed a large mouse colony for animal breeding and genotyping. Maintained cell lines for the laboratory.

•Played a key role in leading a group of 10 MD and PhD fellows which indicated that Viagra safeguards against high blood pressure damage and offers cardiovascular protective effects.

•Honored with a university award that recognized 20 years of dedicated service.

•Review the literature so as to stay on top of cutting edge techniques as well as identify and define specific problems for future studies.

•Stay up to date on current techniques and protocols in the literature and apply it to my studies.

Senior Research Scientist II, Gastroenterology • (11/1991 - 09/2005, 40hrs/wk)

Directed a research laboratory centered on the causative factors of alcoholic liver disease. Led development and optimization of primer and probe design for PCR, Real-time PCR, and Taqman chemistry. Instructed PhD and MD fellows on the application of novel primers and probes. Examined the mechanisms and pathways associated with liver disease.

•Scanning EM in order to discover binding and crystallographic insight for the alpha (I) 1 collagen promoter.

•Studied gene expression analysis for alcoholic liver disease using electromobility shift assays.

•Collect, review, analyze, and prepare biological data for decision-making processes.

•Successfully constructed all of the lab's primers and probes for PCR which supported research resulting in over 20 publications.

•Conduct research experiments including complex techniques and procedures.

•Conduct my own research project in which I am the resident expert.

•Prepare post-doctoral fellows for projects in which they become the expert of on their own studies.

•Actively contributed to research findings that demonstrated the role of alcohol in causing liver disease.

Senior Research Scientist, Epidemiology • (11/1989 - 11/1991, 40hrs/wk)

Orchestrate epidemiological multi-center clinical trials associated with HIV/AIDS. Delivered seminars and scientific meetings at Johns Hopkins University. Managed culture of live HIV virus in P2 and P3 facility. Established and optimized ELISA assays for the detection of HCV.

•Manage large frozen sample repository inventory for epidemiological studies, used to study the natural history of the AIDS virus and other infectious diseases.

•Train staff in the proper techniques for Molecular Biology techniques including DNA/RNA extraction.

•Develop and teach new techniques for use in the laboratory.

•Independently work to resolve experimental research assays.

Scientist, Pulmonary Physiology & Anesthesiology • (11/1985 - 11/1989, 40hrs/wk)

Evaluated the effects of various pharmaceuticals on asthma. Drove in vivo clinical trials on canines.

Brimrose Corp of America, 19 Loveton Cir., Sparks, MD 21152

• (9/2006 - 7/2008, 40hrs/wk)

Laboratory Applications Scientist, Industrial & Pharmaceutical NIR Spectrometry

Deliver training and technical support to customers on NIR (Near-Infrared) spectrometry applications. Assure diligent audit preparation for pharmaceutical companies. Manage software configuration and quality control. Interface with customers at industry tradeshows.

•Author feasibility studies on the use of chemometric modeling as a secondary measurement for thin film coating analysis.

•Offer applications to pharmaceutical, petroleum, agricultural, polymer, chemical, and food industries, refineries, and academics.

BD Diagnostics, 57 Loveton Cir., Sparks, MD 21152

• (7/2010 - 11/2010, 40hrs/wk)

Laboratory Outreach Scientist

Spearhead an HSV Laboratory Outreach study which included initiating relationships with existing and prospective clients. Maintain ongoing communication through continuing education material. Collaborate with clinical lab managers by working closely with laboratory diagnostics and sales personnel.

•Direct the successful launch of an HSV Laboratory Outreach study through relationship building that pinpointed over 300 customers (80%) as potential new sites.

•Significantly surpassed previous efforts which had received minimal response from clients.

MarketAmerica.com, 1302 Pleasant Ridge Rd., Greensboro, NC 27409

• (7/2008 - 2/2013, 40hrs/wk)

Sales Representative / Scientific Consultant

Leverage extensive qualifications in advanced technology, biotechnology, and holistic healthcare to the fields of healthcare and health insurance. Put Nutrametrix in the professional doctor's offices, replacing the pharmaceutical solution that insurance companies promote with the holistic healthcare alternative, which is tailored specifically to the needs of the patient or client and dedicated to providing solutions specifically for health professionals.

•Taught wellness with the opportunity to help the public become more educated about holistic healthcare alternatives to pharmaceuticals.

•Headed our group by putting our product in 50% of the healthcare professional offices visited. This is an unheard of statistic in the field.

•Ran my own business.

UNITED STATES POSTAL SERVICE, York, PA 17402

• (2/2013 – 11/2014, 40hrs/wk)

Sales & Distribution Associate

Perform a variety of sales and customer services at a retail window such as maintaining sufficient inventory of and selling stamps, stamped paper, other retail products and services with use of a computerized system, accept and deliver packages and accountable mail. Issue and cash foreign and domestic postal money orders, accept and respond to customer claims and inquiries, and provide information to the public regarding postal regulations. Handle and process customer purchases and returns relating to products and services with a point of sale system. Conduct product inventories by counting items on hand, accounting for items on display, and verifying and sale records with floor inventory and shrinkage. Rent post office boxes, receive rental payments, conducts reference checks, and complete related forms.

•Sort mail by address and carrier routes, scheme trained; i.e. cross reference 980 possible address items matched to 48 different possible carrier routes, by recall.

•Deliver express mail.

Technical Skills

Animal breeding and genotyping • Antibody isolation and purification • Autoanalyzer for histamine • Automated Liquid Handling experience • Biochemical assays • Chemical industry analysis • Chemometric modeling • Chromatography • Cloning • Confocal microscopy • Densitometry imaging • Dot blot analysis • DNA / RNA preparation • DNA extraction on Automated Qiasymphony • Electromobility Shift Assays (EMSA) • ELISA development • Enzymatic assays • Enzyme analysis • Flourometry • Gas chromatograph • Gel electrophoresis • Gene targeting • Genetic engineering • Image analysis • Lymphocyte isolation • Mass spectrophotometer microscopy • MB (ASCP) certification • Molecular Beacon design • NanoDrop expertise • Nuclear protein preparation • Neonatal myocyte isolation • Nucleic acid labeling • PCR • Petroleum industry • Pharmaceutical industry • Polymer industry • Protein isolation • Protein-DNA interaction • Qiagility Automation for liquid handling • Radiolabeling • Radiation safety monitoring • Real-time PCR • Refineries • Restriction mapping • RNase protection assay • Sample Processing in CAP and CLIA certified clinical laboratory • SDS-page • Sequencing analysis • Site-directed mutagenesis • Southern/Northern/Western blot • Spectrophotometry • Sub-cloning • Taqman chemistry • Thin film coatings • Tissue culture • Transfection assays • Transformation • Vector design and construction • Virus purification

Computer Skills

Adobe Photoshop • FreezerWorks • LDMS • Microsoft Office (Access, Excel, Outlook, PowerPoint, Word) • Molecular biology & protein databases • Primer and probe design software (NCBI) • SAP • Scientific databases & bioinformatics

Selected Publications

2007: Wang Lan; Potter James J; Rennie-Tankersley Lynda; Novitskiy Gennadiy; Sipes Jennifer; Mezey Esteban

Effects of retinoic acid on the development of liver fibrosis produced by carbon tetrachloride in mice.

Biochimica et biophysica acta 2007;1772(1):66-71.

Department of Medicine, The Johns Hopkins University School of Medicine, The Johns Hopkins University, Baltimore, MD 21205-2195, USA. ******@****.***

2005: Mezey Esteban; Rennie-Tankersley Lynda; Potter James J

Effect of leptin on liver alcohol dehydrogenase.

Biochemical and biophysical research communications 2005;337(4):1324-9.

Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205-2195, USA. ******@****.***

2005: Novitskiy Gennadiy; Ravi Rajani; Potter James J; Rennie-Tankersley Lynda; Wang Lan; Mezey Esteban

Effects of acetaldehyde and TNF alpha on the inhibitory kappa B-alpha protein and nuclear factor kappa B activation in hepatic stellate cells.

Alcohol and alcoholism (Oxford, Oxfordshire) 2005;40(2):96-101.

Department of Medicine, 921 Ross Research Building, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205-2195, USA.

2004: Wang Lan; Tankersley Lynda Rennie; Tang Mei; Potter James J; Mezey Esteban

Regulation of alpha 2(I) collagen expression in stellate cells by retinoic acid and retinoid X receptors through interactions with their cofactors.

Archives of biochemistry and biophysics 2004;428(1):92-8.

Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

2004: Potter James J; Rennie-Tankersley Lynda; Mezey Esteban

Leptin deficiency prevents the activation of the murine alpha 2(I) collagen promoter by acetaldehyde.

Archives of biochemistry and biophysics 2004;426(1):73-7.

Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD 21205-2195, USA.

2004: Novitskiy Gennadiy; Potter James J; Rennie-Tankersley Lynda; Mezey Esteban

Identification of a novel NF-kappaB-binding site with regulation of the murine alpha2(I) collagen promoter.

The Journal of biological chemistry 2004;279(15):15639-44.

Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2195, USA.

2004: Mezey Esteban; Potter James J; Rennie-Tankersley Lynda; Caballeria Juan; Pares Albert

A randomized placebo controlled trial of vitamin E for alcoholic hepatitis.

Journal of hepatology 2004;40(1):40-6.

Department of Medicine, 921 Ross Building, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA. ******@****.***

Contact this candidate