Research Associate
Resume:
Sivakumar Gajjeraman, M.Sc., Ph.D.
*** ****** ******, ***#**, Providence, RI 02906
******@*****.*** 312-***-**** (cell) / 401-***-**** (home)
Nationality: Canadian with H1B visa
Experienced research scientist with over ten years of experience in
structural, biochemical, biophysical, microscopic and other spectroscopic
techniques. Extensive hands-on experience on various protein purification
and molecular biology techniques. Proven ability to mentor undergraduate,
master and lab technicians with their research projects. Flexible team
member with excellent analytical and communication skills.
Skills
Molecular biology Biophysics / Biochemistry Cell culture/Computational
DNA construct design X-ray diffraction
Cloning and mutagenesis Enzymatic assays x-ray crystallography data
Membrane/extracellular CD, AFM, TEM and SEM processing
protein expression SEC-MALS MS word, Excel, PYMOL etc.,
protein purification UV/visible spectroscopy
protein chemistry ITC/DLS/Synchrotron FTIR protein structure analysis
protein crystallization Mammalian cell culture
techniques
Qualitative and
quantitative cell biology
assays
Experience
Senior Research Associate
Aug 2013 - present
Department of Molecular Pharmacology, Physiology and Biotechnology,
Brown University, Providence, RI 02903
. Expressed and purified Calcineurin (CN) with the aim of getting the
first CN: substrate complex to elucidate the molecular mechanism of
neurodegenerative diseases at atomic level.
. Purified and crystallized CNA (1-370) CNB (1-170) +A238L RCAN 120
Chimera, diffracted at 2.4 ,
CNA (1-370) CNB (1-170) +A238L RCAN 114Chimera, diffracted at 3.0 and
CNA (1-370) CNB (1-170) + A238L RII Chimera, diffracted at 3.1
. Successfully purified naturally occurring CN mutants, Nuclear DNA
regulatory domains of Protein phosphatase1 Nuclear Targeting Subunit
(PNUTS) using Ni-NTA, ion exchange and size exclusion chromatographic
techniques for structural and biochemical studies
Sr. Research Technologist/Post Doc
Nov 2010 -Aug 2013 Department of Molecular Biosciences, Northwestern
University,
Evanston, IL 60208
. Expressed and purified of mitochondrial inner membrane ABC
transporters ATM1 and ABCB7 using E-coli and Yeast expression system
for structural, biochemical and biophysical studies
. Major Techniques used: HPLC, CD, MALS, DLS, XRD and ATP assays.
Research Associate
Jun 2007- Nov 2010
Department of Chemistry, University of Manitoba, Canada
. Expressed and purified Glycerol Facilitator (GlpF) using Cell free
synthesis for structural, biophysical and functional studies.
. Synchrotron IR spectromicroscopic technique employed for in-situ
investigation on Alzheimer brain tissue samples
. Fabricated 3-D hydrogel in the microfluidic device for cell-cell
interaction with different chemokine.
Post-Doc Research Associate /Research Specialist
Apr 2004-May 2007
Department of Oral Biology, University of Illinois at Chicago, Chicago,
IL
. Designed and fabricated self-assembly based osteoconductive
biomaterials using triblock peptide (LZ-DMP1-LZ) for tissue
regeneration
. Investigated the cytocompatibility of 3-D hydrogel first time with
different cell lines
. Biophysical, biochemical and functional characterization techniques
were employed to understand the mechanism of full length, C and N
terminal Dentin Matrix Proteins (DMP1 and DMP2) during bone and teeth
formation.
. In-vivo and in-vitro mineralization experiments were performed with
Native and Recombinant proteins (DMP1, C and N-terminal DMP1 and
DMP2) and phosphophoryn.
Post-Doctoral Research Associate/Trainee
May 2002- Mar 2004
Department of Material Science and Engineering. University of Florida,
Gainesville,
Department of Biological Sciences, University of Delaware, Newark
. Extracted and purified soluble and insoluble proteins from nacreous
layer of atrina and strombus gigas shells.
. Expressed and purified human Polo like kinases 3 and CIB
Post-Doctoral Fellow (trainee)
Jan 2001- Apr2002
Department of Chemistry and Biochemistry University of Regina, Canada
. Purified, Crystallized human trifunctional enzyme (GART) and
Thioredoxin involved in de novo purine biosynthesis
Ph. D. Graduate student
May 1995-Dec 2000
Crystal Growth Center, Anna University, Chennai, India.
. To understand the etiology of gallstone formation, atherochloresis and
pathological tissue calcification
. Crystallization and characterization of calcium, Magnesium phosphates,
Cholesterol and Calcium oxalates
Education
. Ph.D. Crystal Growth Centre, Anna University, India. US
Equivalency: Ph.D. Major / Specialization: Biological Sciences. (May
1995- Dec 2000)
. M. Sc Physics, University of Madras, Chennai, India (June 1991 -
Sep1993)
. B. Sc Physics, University of Madras, Chennai, India (June 1987- Sep
1990)
Awards
. Post-Doctoral Research fellowship: NSERC sponsored project at
University of Regina Canada (from 2000 - 2002) and NASA sponsored
project fellowship at University Florida, USA (2002 - 2004)
. Senior Research Fellow awarded by Council of Scientific and Industrial
Research, Government of India, New Delhi, India (1997 -2000)
. Research Fellowship awarded by Crystal Growth Center, Anna University,
India (1995-1997)
. Academic Excellence award - High School (1985) and Higher Secondary
School (1987)
List of Publication
Manuscripts with submission within next 90 days: 2
. Effect of a protein osmolyte on the stability of the integral membrane
protein Glycerol Facilitator
Simon Baturin, Jamie J. Galka, Piyadasa H, S. Gajjeraman and Joe D.
O'Neil Biochemistry and Cell Biology 2014
. Growth and Positioning of Adipose-Derived Stem Cells in Microfluidic
Devices
N. Wadhawan, H. Kalkat, X.L, Gajjeraman, S. Nandagopal, N.
Hao, M. Zhang, J.X. Deng, B. Xiang, S. Mzengeza, D. Freed, R. Arora,
G.H. Tian and F. Lin.
Lab on a Chip, 2012, 12 (22), 4829 - 4834.
. Association between amyloid plaque, lipid, and creatine in hippocampus
of TgCRND8 mouse model for Alzheimer disease
Alexandra Kuzyk, Marzena Kastyak, Veena Agrawal, Meghan Gallant,
Gajjeraman Sivakumar, Margaret Rak, Marc R. Del Bigio, David Westaway,
Robert Julian, and Kathleen Gough,
Journal of Biological Chemistry, 2010, 285(41), 31202-7
. Biological Assemblies Provide Novel Templates for the Synthesis of Bio
composites and Facilitate Cell Adhesion
Sivakumar Gajjeraman, Gen He, Karthikeyan Narayanan, Anne George.
Advanced Functional Materials, 2008, 18, 1-9.
. Matrix macromolecules in hard tissues controls the nucleation and
hierarchical assembly of hydroxyapatite:
Sivakumar Gajjeraman, Karthikeyan Narayanan, Jianjun Hao, and Anne
George,
Journal of Biological Chemistry, 2007, 282 (2) 1193-1204.
. Dentin Matrix Protein 1 Regulates Dentin Sialophosphoprotein Gene
Transcription during Early Odontoblast Differentiation
Karthikeyan Narayanan, Sivakumar Gajjeraman, Amsaveni Ramachandran,
Jianjun Hao and Anne George. Journal of Biological Chemistry, 2006,
281, 19064 - 19071
. Spatially and Temporally Controlled Bio mineralization is facilitated
by Interaction between Self-Assembled Dentin Matrix Protein 1 and
Calcium Phosphate Nuclei in solution
Gen He, Sivakumar Gajjeraman, David Schultz, David Cookson, Chunlin
Qin William T. Butler, Jianjun Hao, and Anne George.
Biochemistry, 2005, 44 (49), 16140 - 16148.
. Nano-Fibrous Calcite synthesized via a Solution-Precursor-Solid (SPS)
Mechanism
Matthew Olszta, Sivakumar Gajjeraman, Michael Kaufman, Laurie Gower.
Chem Materials 16-200*-****-****.
. Knoop micro hardness studies of urinary calculi and pure calcium
oxalate crystals
E.K.Girija, G.R.Sivakumar, S.Narayana Kalkura, P.Ramasamy, D.R.Joshi,
P.B.Sivaraman.
Materials Chemistry and Physics, 63 (2000) 50 - 54.
. Effect of magnesium on the crystallization and the microhardness of
dicalcium Phosphate dihydrate
G.R.Sivakumar, S.Narayana Kalkura and P.Ramasamy
Materials Chemistry and Physics, 57 (1999) 238 - 243.
. Crystallization and characterization of calcium phosphates: Brushite
and Monetite
G.R.Sivakumar, E.K.Girija, S.Narayana Kalkura and C. Subramanian
Crystal Research and Technology, 33 (1998) 195 - 203.
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