Sivakumar Gajjeraman, M.Sc., Ph.D.

*** ****** ******, ***#**, Providence, RI 02906

acow5s@r.postjobfree.com 312-***-**** (cell) / 401-***-**** (home)

Nationality: Canadian with H1B visa

Experienced research scientist with over ten years of experience in

structural, biochemical, biophysical, microscopic and other spectroscopic

techniques. Extensive hands-on experience on various protein purification

and molecular biology techniques. Proven ability to mentor undergraduate,

master and lab technicians with their research projects. Flexible team

member with excellent analytical and communication skills.

Skills

Molecular biology Biophysics / Biochemistry Cell culture/Computational

DNA construct design X-ray diffraction

Cloning and mutagenesis Enzymatic assays x-ray crystallography data

Membrane/extracellular CD, AFM, TEM and SEM processing

protein expression SEC-MALS MS word, Excel, PYMOL etc.,

protein purification UV/visible spectroscopy

protein chemistry ITC/DLS/Synchrotron FTIR protein structure analysis

protein crystallization Mammalian cell culture

techniques

Qualitative and

quantitative cell biology

assays

Experience

Senior Research Associate

Aug 2013 - present

Department of Molecular Pharmacology, Physiology and Biotechnology,

Brown University, Providence, RI 02903

. Expressed and purified Calcineurin (CN) with the aim of getting the

first CN: substrate complex to elucidate the molecular mechanism of

neurodegenerative diseases at atomic level.

. Purified and crystallized CNA (1-370) CNB (1-170) +A238L RCAN 120

Chimera, diffracted at 2.4 ,

CNA (1-370) CNB (1-170) +A238L RCAN 114Chimera, diffracted at 3.0 and

CNA (1-370) CNB (1-170) + A238L RII Chimera, diffracted at 3.1

. Successfully purified naturally occurring CN mutants, Nuclear DNA

regulatory domains of Protein phosphatase1 Nuclear Targeting Subunit

(PNUTS) using Ni-NTA, ion exchange and size exclusion chromatographic

techniques for structural and biochemical studies

Sr. Research Technologist/Post Doc

Nov 2010 -Aug 2013 Department of Molecular Biosciences, Northwestern

University,

Evanston, IL 60208

. Expressed and purified of mitochondrial inner membrane ABC

transporters ATM1 and ABCB7 using E-coli and Yeast expression system

for structural, biochemical and biophysical studies

. Major Techniques used: HPLC, CD, MALS, DLS, XRD and ATP assays.

Research Associate

Jun 2007- Nov 2010

Department of Chemistry, University of Manitoba, Canada

. Expressed and purified Glycerol Facilitator (GlpF) using Cell free

synthesis for structural, biophysical and functional studies.

. Synchrotron IR spectromicroscopic technique employed for in-situ

investigation on Alzheimer brain tissue samples

. Fabricated 3-D hydrogel in the microfluidic device for cell-cell

interaction with different chemokine.

Post-Doc Research Associate /Research Specialist

Apr 2004-May 2007

Department of Oral Biology, University of Illinois at Chicago, Chicago,

IL

. Designed and fabricated self-assembly based osteoconductive

biomaterials using triblock peptide (LZ-DMP1-LZ) for tissue

regeneration

. Investigated the cytocompatibility of 3-D hydrogel first time with

different cell lines

. Biophysical, biochemical and functional characterization techniques

were employed to understand the mechanism of full length, C and N

terminal Dentin Matrix Proteins (DMP1 and DMP2) during bone and teeth

formation.

. In-vivo and in-vitro mineralization experiments were performed with

Native and Recombinant proteins (DMP1, C and N-terminal DMP1 and

DMP2) and phosphophoryn.

Post-Doctoral Research Associate/Trainee

May 2002- Mar 2004

Department of Material Science and Engineering. University of Florida,

Gainesville,

Department of Biological Sciences, University of Delaware, Newark

. Extracted and purified soluble and insoluble proteins from nacreous

layer of atrina and strombus gigas shells.

. Expressed and purified human Polo like kinases 3 and CIB

Post-Doctoral Fellow (trainee)

Jan 2001- Apr2002

Department of Chemistry and Biochemistry University of Regina, Canada

. Purified, Crystallized human trifunctional enzyme (GART) and

Thioredoxin involved in de novo purine biosynthesis

Ph. D. Graduate student

May 1995-Dec 2000

Crystal Growth Center, Anna University, Chennai, India.

. To understand the etiology of gallstone formation, atherochloresis and

pathological tissue calcification

. Crystallization and characterization of calcium, Magnesium phosphates,

Cholesterol and Calcium oxalates

Education

. Ph.D. Crystal Growth Centre, Anna University, India. US

Equivalency: Ph.D. Major / Specialization: Biological Sciences. (May

1995- Dec 2000)

. M. Sc Physics, University of Madras, Chennai, India (June 1991 -

Sep1993)

. B. Sc Physics, University of Madras, Chennai, India (June 1987- Sep

1990)

Awards

. Post-Doctoral Research fellowship: NSERC sponsored project at

University of Regina Canada (from 2000 - 2002) and NASA sponsored

project fellowship at University Florida, USA (2002 - 2004)

. Senior Research Fellow awarded by Council of Scientific and Industrial

Research, Government of India, New Delhi, India (1997 -2000)

. Research Fellowship awarded by Crystal Growth Center, Anna University,

India (1995-1997)

. Academic Excellence award - High School (1985) and Higher Secondary

School (1987)

List of Publication

Manuscripts with submission within next 90 days: 2

. Effect of a protein osmolyte on the stability of the integral membrane

protein Glycerol Facilitator

Simon Baturin, Jamie J. Galka, Piyadasa H, S. Gajjeraman and Joe D.

O'Neil Biochemistry and Cell Biology 2014

. Growth and Positioning of Adipose-Derived Stem Cells in Microfluidic

Devices

N. Wadhawan, H. Kalkat, X.L, Gajjeraman, S. Nandagopal, N.

Hao, M. Zhang, J.X. Deng, B. Xiang, S. Mzengeza, D. Freed, R. Arora,

G.H. Tian and F. Lin.

Lab on a Chip, 2012, 12 (22), 4829 - 4834.

. Association between amyloid plaque, lipid, and creatine in hippocampus

of TgCRND8 mouse model for Alzheimer disease

Alexandra Kuzyk, Marzena Kastyak, Veena Agrawal, Meghan Gallant,

Gajjeraman Sivakumar, Margaret Rak, Marc R. Del Bigio, David Westaway,

Robert Julian, and Kathleen Gough,

Journal of Biological Chemistry, 2010, 285(41), 31202-7

. Biological Assemblies Provide Novel Templates for the Synthesis of Bio

composites and Facilitate Cell Adhesion

Sivakumar Gajjeraman, Gen He, Karthikeyan Narayanan, Anne George.

Advanced Functional Materials, 2008, 18, 1-9.

. Matrix macromolecules in hard tissues controls the nucleation and

hierarchical assembly of hydroxyapatite:

Sivakumar Gajjeraman, Karthikeyan Narayanan, Jianjun Hao, and Anne

George,

Journal of Biological Chemistry, 2007, 282 (2) 1193-1204.

. Dentin Matrix Protein 1 Regulates Dentin Sialophosphoprotein Gene

Transcription during Early Odontoblast Differentiation

Karthikeyan Narayanan, Sivakumar Gajjeraman, Amsaveni Ramachandran,

Jianjun Hao and Anne George. Journal of Biological Chemistry, 2006,

281, 19064 - 19071

. Spatially and Temporally Controlled Bio mineralization is facilitated

by Interaction between Self-Assembled Dentin Matrix Protein 1 and

Calcium Phosphate Nuclei in solution

Gen He, Sivakumar Gajjeraman, David Schultz, David Cookson, Chunlin

Qin William T. Butler, Jianjun Hao, and Anne George.

Biochemistry, 2005, 44 (49), 16140 - 16148.

. Nano-Fibrous Calcite synthesized via a Solution-Precursor-Solid (SPS)

Mechanism

Matthew Olszta, Sivakumar Gajjeraman, Michael Kaufman, Laurie Gower.

Chem Materials 16-200*-****-****.

. Knoop micro hardness studies of urinary calculi and pure calcium

oxalate crystals

E.K.Girija, G.R.Sivakumar, S.Narayana Kalkura, P.Ramasamy, D.R.Joshi,

P.B.Sivaraman.

Materials Chemistry and Physics, 63 (2000) 50 - 54.

. Effect of magnesium on the crystallization and the microhardness of

dicalcium Phosphate dihydrate

G.R.Sivakumar, S.Narayana Kalkura and P.Ramasamy

Materials Chemistry and Physics, 57 (1999) 238 - 243.

. Crystallization and characterization of calcium phosphates: Brushite

and Monetite

G.R.Sivakumar, E.K.Girija, S.Narayana Kalkura and C. Subramanian

Crystal Research and Technology, 33 (1998) 195 - 203.

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