molecular&cell biology, stem cell, oncology, metabolism, pharmacology

Li Ma, Ph. D.

****.********@*****.*** • 513-***-**** (c)

**** ***** *********, **** **, San Diego, CA 92126

Objective:

• Highly motivated scientist with strong track record of original publications in high impact journals, such as CELL and NATURE, seeking a scientist position in both pharmaceutical and biotech industry.

Summary of qualifications and skills

• Multiple years of extensive expertise in biomedical fields of molecular and cell biology,

stem cell biology, oncology, metabolism, pharmacology and Toxicology.

• Hands-on experience in cell culture (primary cells, cancer cells and stem cells such as

iPS and ESC), cellular and molecular skills, cell-based assay development, fluorescence

imaging, Flow Cytometry, scientific statistical analysis, etc.

• Broad hands-on disease modeling experience in cells, murine and non-murine animals,

including aging, surgical diseases model, cancers, xenograft etc.

• Strong capability of detailed thinking, problem solving and organizational skills

• Excellent presentation and communication skills with teaching experience, self-starter

and team player.

• Strong track record of original publications.

Technical Experience

Cell Biology

Primary and immortalized cell culture

Stem cells culture (iPS & ESC), EB formation, cell differentiation and characterization

Primary adult stem cells isolation,

Conventional light and confocal fluorescence microscopy

Immunohistochemistry (IHC), immunocytochemistry (IC), immunofluorescence (IF)

Paraffin and frozen section preparation

Tissue microarray (TMA) staining and data analysis

Fluorescent activated cell sorting and flow cytometry

Proliferation assays (BrdU, MTT, Dye exclusion assays)

Other cell-based assays: apoptosis, senescence, ROS, colony formation, invasion, etc

Mitochondria staining and function assay

YSI metabolites analysis

Seahorse extracellular flux analysis (XF24 and XF96)

Metabolic labeling assay and data analysis (Gas chromatography/Mass spectrometry,

Liquid chromatography/Mass spectrometry)

Molecular Biology and Biochemical

Basic recombinant DNA techniques, Site-directed mutagenesis, gene cloning

DNA purification, Gel electrophoresis

RNA purification, in vitro transcription, qPCR and PCR

Viral transduction (lentiviral and adenoviral) of primary cell lines

shRNA, siRNA, RNAi and plasmid transfections and transductions

Fluorescent in situ hybridization

mRNA array, miRNA array, miRNA qPCR, RNA-seq

Knowledge of bioinformatics tools and programs

Protein expression, separation and purification

Protein staining, Western blotting analysis, Immunoprecipitation (IP)

Enzyme activity assay, protein phosphorylation analysis

ELISA and luciferase assay

Animal Handling

Proficient in mouse handling and drug administration

Tail genotype screening

Mouse models (multiple cancer models/ Xenograft /aging/endometriosis, etc.)

Praxeology experiment

Anesthesia, injection and surgery

Dissection and sample preparation

Pharmacokinetic & pharmacodynamic (PK/PD) studies

Professional Software

FACS Diva, CellQuest, FlowJo, Confocal FluoView-1000, Image J

PrismGraph, Photoshop, Core Draw, Adobe llustrator, etc

Research Experiences

Research Associate: with Dr. Juan Carlos Izipisua Belmonte, Professor

(March’2013- present) Salk Institute for biological studies

• Genome-Scale CRISPR-mediated Screening of targeting gene which can rejuvenate

senescence phenotype of the differentiated MSCs from HGPS patient iPS (Hutchinson–

Gilford progeria syndrome, HGPS)

• Screening of targeting gene which links to rejuvenation of stress/passage induced aging

process in primary mesenchymal stem cells (AD-MSC & BW-MSC) with Cas9 system

• Metabolic profiling of differentiated disease HSC and Cord blood-HSC

• Capturing an alternative primed pluripotent stem cells from mammalian species

• Reprogramming human cells to Brain Tumor Initiating-like cells

• Study on Hypoxia induces tumor-initiating capabilities in breast cancer through

regulation of Ten-eleven translocation (TET)

• Study on The Central Carbohydrate Metabolic Network in premature aging using a

novel model of Hutchinson–Gilford progeria syndrome

Postdoctoral Associate: with Dr. Jorge Moscat, Professor

(Jan’2011-Mar’2013) NCI Cancer Center, Sanford-Burnham Medical Research Institute;

(Jan’2009-Jan’2011) The Vontz Center for Molecular Studies, University of Cincinnati

• Research on novel signaling networks that influence cell metabolism and allow cancer

cells to proliferate under stress microenvironment

• Study on the mechanism of activation and action of the tumor suppressor PKCζ in

metabolic reprogramming during nutrient deprivation

• Study of molecular mechanisms of PCNA and PCNA-phosphorylation controlling cell

proliferation and tumor development in prostate cancer

• Receptor tyrosine kinase signaling and cell cycle regulation mechanisms in cancer cell

proliferation, metastasis, and the response to chemotherapeutic agents

Ph D. / Research assistant: With Dr. Lin Cao, Professor

(Sep’2005-Jul’2008) School of Pharmacy, Fudan University, Shanghai, China.

• Thesis: Mechanistic studies of Nomegestrol microparticle on Endometriosis

(Sep’2005-Dec’2008) Shanghai Institute of Planned Parenthood Research, Shanghai, China.

• Involved in Pharmacokinetics, pharmacodynamics, and toxicology projects in different

animal models, working with pharmaceutical industries

Education

• Ph.D. Pharmacology

School of Pharmacy, Fudan University, Shanghai, China. Jul’2005- Sep’2008.

• M.S. Biochemistry Genetics

• B.S. Biology Education.

Xuzhou Normal University, Xuzhou, China. Jul’1998- Sep’2005.

Selected recent publications:

1. Wu M.-Z., Chen S.-F., Nieh S., Benner C., Ger L.-P., Jan C.-I., Ma L., Chen C.-H., Hishida

T., Chang H.-T., Lin Y.-S., Montserrat N., Gascon P., Sancho-Martinez I., and Belmonte

J.C. Hypoxia-induced TET-TNFα-p38-MAPK signaling axis drives tumor malignancy of

human breast cancer. Cancer research, under revision.

2. Jun Wu, Daiji Okamura, Mo Li, Keiichiro Suzuki, Chongyuan Luo, Li Ma, Yupeng He,

Zhongwei Li, Chris Benner, Isao Tamura, Marie Krause, Joseph R. Nery, Tingting Du,

Zhuzhu Zhang, … and Juan Carlos Izpisua Belmonte. (2015) A spatially defined

pluripotent state confers interspecies chimera competency. Nature, in press.

3. Ma, L., Tao, Y., Duran, A., Llado, V., Galvez, A, Barger, J.F., Castilla, E.A., Chen, J.,…

Diaz-Meco, M.T., Richardson, A.D., and Moscat, J. (2013) Control of nutrient stress-

induced metabolic reprogramming by PKCζ in tumorigenesis. Cell, 152, 599-611.

4. Zhao H, Lo YH, Ma L, Waltz SE, Gray JK, Hung MC, Wang SC. (2011). Targeting

tyrosine phosphorylation of PCNA inhibits prostate cancer growth. Molecular Cancer

Therapeutics. Jan 10(1): 29-36.

5. Xie S, Zhu Y, Ma L, Lu Y, Zhou J, Gui Y, Cao L. (2010). Genome-wide profiling of gene

expression in the epididymis of α-chlorohydrin-induced infertile rats using an

oligonucleotide microarray. Reproductive Biology and Endocrinology. Apr22, 8: 37.

6. Ma L, Xie S, Zhu Y, Lu Y, Cao L (2008). Establishment and Histological Observation of a

New Mouse Model of subcutaneous Endometriosis. Acta Laboratorium Animalis Scientia

Sinica. 16(3): 192-195.

7. Xie S, Ma L, Liu X, Li Z, Wang L, Li D, Sun Z, Cao L (2008). Alteration of Rat Sperm

Maturation and Fertility Induced by Dutasteride Treatment. Acta Laboratorium Animalis

Scientia Sinica, 15(4): 308-312.

8. Patent: XIAO L, CHEN Q, MA L, CAO L, et al. (2011,CN101612111B) Preparation and

usage of the Microspheres of Nomegestrol acetate and/or its Analogues.

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