Training Engineer

KURT RETZLOFF

**** ********* ***, **** **** City UT, 84108 801-***-****

****.********@*****.***, http://www.linkedin.com/in/kurtretzloff/

Seeking Full Time work in Chemical/Biological Molecular Diagnostics and

Analytics.

EDUCATION

B.S. Chemical Engineering 2017, (estimated graduation)

University of Utah,

Junior Level Coursework: Heat Transfer, Fluid Dynamics, Petroleum

Geoscience, and Numerical Methods in MATLAB

B.S. Chemistry 2012 - ACS Certified

University of Utah

B.S. Biology 2007

University of Utah

A.S. Mechanical Engineering

Utah Valley University

WORK EXPERIENCE

Lab Technician I / Molecular Diagnostic Research, September 2014 - January

2015,

Department of Orthopedic Surgery at University of Utah School of Medicine

. Tissue culture, qPCR, RNA isolation cryopreservation and Delta Delta Ct

analysis for over 300 primary human tissue culture samples resulting

discovery of upregulation and downregulation in 5 genes.

. Trained resident surgeon and an undergraduate in qPCR methods.

Metallurgical / Chemical Engineer Intern, January 2013-May 2013,

Rio Tinto Minerals, Kennecott Utah Copper Concentrator

. Conducted Paired t-test analysis on XRF data between central lab and

metallurgical lab resulting in questioning reproducibility of results.

. Six 5-minute float tests performed per day for use in PI program data

records.

. Volunteered to assist in plant floatation cell inspections of impeller

blades, darts, down tubes, skirts, reagent pipes, floor venting, build up

etc. during down-time resulting in recommending repairs.

. MSHA and OSHA one week training.

Lab Technician I / Molecular Diagnostic Research, August 2008 - August

2012,

Department of Surgery Research at University of Utah School of Medicine

. Replaced small Swiss motor of a high precision real time PCR Roche light

cycler, resulting in $20,000 saved in work hours and outsourced equipment

repair.

. Performed qPCR and calculated DeltaDelta Ct's for pancreatic cancer genes

resulting in disease trends being published.

. Transformed, amplified and purified plasmids used for genetic expression

in rats.

. Trained medical students in molecular biology protocols.

. Created viral titer plaque assay resulting in highly concentrated

virulent strain.

. Tissue culture and cryopreservation successfully performed for over 10

cell lines.

. Used Vector NTI software and database to construct plasmids and plasmid

libraries.

. Maintained 125 cages, racks, water, food and documentation for immune

compromised mice and rats by IACUC standards.

Lab Aide, Molecular Diagnostic Research, October 2006 - July 2008,

Department of Human Genetics at University of Utah

. Chemically stimulated mouse kidney tissue cultures inducing a

hypertensive response.

- Data revealed dose dependent disease states and published in journal.

. Used flow cytometry machinery to isolate and count fluorescently tagged

cells.

- Cells verified by microscopy and used as primary tissue culture

cell lines.

. UROP minority scholarship used to fun one semester's salary.

- Poster presented on "Angiotensin I Stimulated Renin Expression"

Lab Aide, Kropf Lab at University of Utah

. Contributed rationale on why triangular skeletal growth patterns exist in

brown alga.

- Used references to engineering principles from statics and dynamics

coursework.

- Glassware cleaning and restocking and Arabidopsis tagging.

SKILLS, TECHNIQUES & TRAINING

Petroleum Field Training:

Uintah Basin Applied Technical College, October 2013,

- Petroleum Technology Program H2S hazardous material certification by

Mike Mold.

- IADC Well Cap Control - half day introductory simulation Drilling

course.

- Newfield Exploration Co. drill rig tour

- Crude oil processing and loading bay tour.

Chemical:

Burette,

Calibration Curve, Calorimetry,

Cancer Drug Synthesis,

ELISA,

Error Propagation, Flame Test,

Gravimetric Analysis, Heat Exchanger Analysis,

HNMR,

HPLC,

Mass Spectroscopy, Material Balancing, Inorganic Synthesis, Internal

Standards, LOD,

LOQ,

Matrix Effects, Measurement uncertainty,

NIST Chemistry,

Normalizing,

Organic Synthesis,

pH meter,

Quantitative Analysis, Refluxing,

Rotovap,

Sample Preparation and Analysis,

Sensitivity,

Standard Addition, Standard Deviations, Systematic and Random Error

Analysis,

Flash Separation

Titrations,

TLC,

Volumetric Flask,

Wet Chemistry,

WebBook.

Computer Software/Databases:

Aspen Plus,

Basic,

C++,

CAD/CAM,

Comsol,

Excel,

Fluent,

Gambit,

Java,

MATLAB,

MicroStation,

PI database (proprietary to Kennecott Copper),

Polymath,

Wolfram.

Metallurgical:

Cleaning Tanks,

Copper Concentration,

Density Analysis training,

Denver use,

Filtration,

Float Tests,

Denver Impeller replacement,

Micelle technology,

Ore Pellet Preparation, Molybdenum Concentration,

MSHA,

On Stream Analyzer (XRF),

Oven,

Ring and Puck Pulveriser,

Paired t-test analysis, Riffle Splitter,

Slurry Sampling,

XRF Analysis,

Copper-Moly cell chamber inspections, 330,000 tons overburden processed per

day.

Safety Training:

Blood Borne Pathogens,

CITI (Human Subject Research Training),

H2S Safety Certification

IACUC,

IBC,

IRB,

Laboratory Exposure Control Plan,

MSHA,

OSHA,

University of Utah Biological Hazards Program.

Foreign Language: Spanish (Fluent)

Service: Utah Youth Village, 2004-2013, Certified treatment parents for 15

individual children in crisis.

Physical Training: Swim, Bike, Run, Weight Lifting, for Recreational

Competition.

Contact this candidate