Medical Clinical

Gladson Muthian Ph.D.

**** ******** ****

Nashville, TN 37204

Email: ********@***.***

**************@*****.***

Cell Phone: +1-615-***-****

Res :( +1-615-***-****

Lab :( +1-615-***-**** Visa Status: Green card holder (US permanent resident)

Skills Summary:

A great deal of experience with handling clinical Sample analysis, Micro array (PGD), sperm

morphology, sperm count, implantation study, medium preparation, cryopreservation, egg freezing

and thawing, Radio immune assay of hormones, (Steroid hormones and peptide hormones)

Surgical removal of Endocrine Glands in mice, rats and monkeys (Vasectomy, ovariectomy,

Thyroidectomy, Adrenalectomy, Gonadectomy) biochemical analysis, Florescence Microscope,

Cryopreservation, Expertise in Cell culture, blastocyst culture,cryostat- Tissue sectioning, an

clinical data related to Case Management and Disease Management Knowledge of Work

Instruction (WI) and Standard Operating Procedure (SOP) Excellent writing skills, verbal

communication abilities and strategic thinking skills. Ability to work independently and manage

time appropriately Good negotiation and decision-making skills

Additional skills:

Research skills include Embryology, Developmental biology, Immunology, Biochemistry,

Andrology,Reproductive Endocrinology, (Male and female Reproduction), Pre implantation

Genetics Diagnosis, cancer biology, Neurology, Bio statistical Data Analysis, proficient with

Microsoft Word, Excel and Graph pad Prism, Power point presentation, Star panel, Cyto vision

imaging (Karyogram) and Manuscript writing for publication, etc.

Management: Effective communication skills with high confidence in presentation to senior

personnel. Co-operative and highly motivated team player

License Certification (ASCP) : Cytogenetics (CG)

Professional Membership:

Member for society for neuroscience

Life Member, Indian Society for Reproductive Biology and Comparative Endocrinology, Chennai,

India.

Member for Endocrine Society, USA.

Member for Society for the study of Reproduction, USA.

Member for American Association of Cancer (USA).

PhD Thesis title: Interaction of estrogen, progesterone and corticosterone acetate on uterine and

cervical collagen and glycosaminoglycans in rats.

M.Phil. thesis title: Effect of vasectomy on rat testis and epididymis.

Education:

Ph.D, University of Madras, India, Endocrinology, 1998.

M Phil, University of Madras, India, Endocrinology, 1990.

MSc, Madurai Kamaraj University, India, Zoology, 1988.

BSc, Madurai Kamaraj University, India, Zoology, 1984.

Professional Experience:

Fertility clinic associated with Genetic associate: Pre implantation Genetics Diagnosis

Laboratory, Nashville Fertility Clinic, Nashville TN

Special Analyst: 2014 – present

As a special research analyst responsible for conducting complex diagnostic test from

single human embryonic cells isolated from human blastocysts. It involves detection of

genetic abnormalities including aneuploidy in human embryonic cells by whole genome

amplification and array hybridization using commercially available kit.

Responsible to maintain patients clinical sample and analysis, maintaining clinical records

Meharry Medical College, Dept. of Neuroscience and Pharmacology, Nashville, TN

Research Associate: 2010- 2014

As a special research analyst responsible for conducting complex diagnostic test from

single human embryonic cells isolated from human blastocysts. It involves detection of

genetic abnormalities including aneuploidy in human embryonic cells by whole genome

amplification and array hybridization using commercially available kit.

Responsible to maintain patients clinical sample and analysis, maintaining clinical records

Postdoctoral fellow: 2006-2010

• Responsible for maintaining the lab, the lab animals, lab record note book,

maintenance of laboratory safety records for chemicals and radioactivity use,

trained Research Assistants, Research fellows and Postdoctoral fellows

• Completed several research projects (Muthian et al 2010 and In

preparation).Executed experiments in various projects with minimal supervision.

• Obtained input from clinical team for clinical data management issues.

• Generated reports for Clinical Events and Protocol Deviations for Regulatory

Affairs.

• Participated consistently in complaint investigations, corrective and preventative

actions.

• Reviewed data effectively for completeness and inconsistencies.

• Issued and managed clinical data queries to closure.

• Identified process improvements for clinical data collection.

• Recognized and identified possible revision in Work Instruction (WI) and Standard

Operating Procedure (SOP).

• Prepared and created templates for period clinical progress reporting and

populating templates with clinical data.

• Handling clinical sample and clinical sample analysis, clinical data analysis

• Research on female reproduction (uterine Implantation, abortion)

Vanderbilt University Medical School, Dept. of Neurology, Nashville, TN

Postdoctoral Fellow: 2001-2006

• Responsible for training postdoctoral research fellows and research assistants.

• Completed several research projects (Muthian et al).

• Presented research findings at various international conferences.

• Executed experiments in different projects with minimal supervision (Muthian et al

2004, 2006).

• Maintained of laboratory records.

• Statistical Data Analysis.

. Clinical sample collection and biochemical estimation in clinical blood sample and animal

samples.

All India Institute of Medical Sciences, Dept. of Reproductive Biology, New Delhi, India.

Research Officer: 1999-2001

• Responsible for training a research assistant.

• Completed research project related to plant compound and uterine bleeding.

• Executed experiments in different projects.

• Data collection and data analysis. Clinical blood sample analysis

University of Madras, Dept. Of Endocrinology and Human Reproduction, Madras, India.

Junior Research Fellow: 1991-1993

Responsible for maintaining the lab, the lab animals, lab record note book, maintenance of

laboratory safety records for chemicals and radioactivity use, trained Research Assistants, M. Phil

Students, M.Sc. Students,

Responsible for doing routine hormone assay in human sample and animal sample.

University of Madras, Dept. of Endocrinology and human Reproduction, Madras, India.

Senior Research Fellow: 1994 – 1998

Responsible for maintaining the lab, the lab animals, lab record note book, maintenance of

laboratory safety records for chemicals and radioactivity use, trained Research Assistants, M. Phil

Students, M.Sc. Students and junior research fellows,

Responsible for doing routine hormone assay in human blood sample and animal sample.

Biochemical estimation in blood and sperm sample.

Clinical sample collection, Clinical data analysis

Publications:

1. Smith ML1, King J2, Dent L1, Mackey V1, Muthian G1, Griffin B1, Charlton CG3.Effects of

acute and sub-chronic l-dopa therapy on striatal l-dopa methylation and dopamine

oxidation in an MPTP mouse model of Parkinson's disease. (Life science)

2. Gladson Muthian, Jennifer King, Lemuel Dent, Marquitta Smith, Veronica Mackey and

Clivel Charlton (2012) Prenatal and Postnatal exposures to 1-Methyl-4-Phenyl-1,2,3,6-

tetrahydropyridine (MPTP) impaired mouse midbrain dopamine system and may produce

predisposing and inducing model for Parkinson’s disease. Journal of Behavioral and

Brain science, 2: 485 – 494.

3. Veronica R. Mackey, Gladson Muthian, Marquitta Smith, Jennifer King and Clivel G.

Charlton (2012) Prenatal exposure to methanol on nigrostriatal dopamine system in

C57BL/6J mice Life Sciences, 91: 921- 927.

4. Jennifer M. King, Muthian G, Mackey V, Smith M, Charlton C (2011)

Dihydroxyphenylalanine modulates the steady-state expression of mouse striatal

tyrosine hydroxylase, aromatic -amino acid decarboxylase, dopamine and its

metabolites in an MPTP mouse model of Parkinson's disease. Life sciences, 24:

638-43.

5. Muthian G, Mackey J, KING J and Charlton CG (2010) Modeling a sensitization stage and

a precipitation stage for Parkinson’s disease using prenatal and postnatal 1-Methyl-4-

phenyl 1,2,3,6 – tetrahydropyridine administration. Neuroscience, 169: 1085- 1093.

6. Himanshu P. Raikwar, Gladson Muthian, Johnson Rajasingh, Caroline N. Johnson

and John J. Bright (2006). PPARγ antagonists reverse the inhibition of neural antigen-

specific Th1 response and experimental allergic encephalomyelitis by Ciglitazone and 15-

Dehydroxy- Delta (12, 14) – ProstaglandinJ. J.Neuro Immunology, 178: 76-86.

7. Muthian G, Chellappan G. Pradeep, Sargapradeep K, Kaleysaraj R, and Bright JJ.

(2006). Setaria digitata Secreted filarial lipids modulate IL-12 signaling through JAK- STAT

pathway leading to the development of Th1 response. Experimental parasitology, 114:

193-203.

8. Muthian G, Raikwar HP, Rajasingh J, and Bright JJ. (2006). Vitamin D ameliorates

experimental allergic encephalomyelitis by blocking IL12 signaling through JAK STAT

pathway in T cell lymphocytes. Neurosci Research, 83: 1299 – 1309.

9. Muthian G, Himanshu P. Raikwar, Caroline Johnson, Rajasingh Johnson, Amit

Kalautkar, Lawrence J. Marnett and John J Bright (2006). The effect of COX2 inhibitors in

EAE by blocking IL-12 signaling through JAK –STAT path way in T cell lymphocytes. J.

Clin Immunology, 26(1) 73-8).

10. Rajasingh J, Raikwar HP, Muthian G, and Bright JJ (2006). Curcumin induces

growth arrest by blocking the constitutive activation of JAK-STAT signaling pathway in adult

Tcell Leukemia. Bioche Biophys Res Comm, 340:359-36).

11. Raikwar HP, Muthian G, RajaSingh J, Johnson C, and Bright JJ (2005). PPAR

gamma antagonists exacerbate neural antigen-specific Th 1 response and experimental

allergic encephalomyelitis. J. Neuro Immunology, 167: 99-107.

12. Muthian G and Bright JJ (2004). Quercetin ameliorates experimental allergic

encephalomyelitis by blocking IL-12 signaling through JAK-STAT pathway in T cell

lymphocytes. J.Clin Immunology, 24: 542- 552.

13. Bright JJ, Natarajan C, Sriram S, and Muthian G (2004). IL-3- induced activation of

JAK2-STAT5 signaling pathway in microglia leads to CNS inflammation, demyelination and

motor dysfunction in GFAP-IL-3 transgenic mice. Glia, 45: 188-196.

14. Natarajan C, Muthian G, Barak Y, Evans RM, and Bright JJ (2003). Peroxisome

proliferators activated receptor-gamma deficient heterozygous mice develop an

exacerbated neural antigen induced Th1 response and experimental allergic

encephalomyelitis. J. Immunology, 171: 5743 –5750.

15. Kumar A, Gladson M, Ahamed N, and Tyagi, K (2002). Estrogenic effects of an

Ayurvedic Polyherbal formulation: Ashokarishta; Singapore J. Obstetrics& Gynecology,

33: 14-23.

16. Gladson M, Arunakaran J, Malini T, Govidarajulu P, and Srinivasan N

(2000).Interactions of ovarian hormones and adrenal hormone on uterine

glycosaminoglycans in rats. Mol and Cell Endocrinology, 164: 267.

17. Gladson M, Govindarajulu P and Srinivasan N (1999). The effect of hypo and

hyper thyroidism on uterine and cervical collagen and glycosidases in albino rats.

Endocrine Res, 25: 129-137.

18. Gladson M, Srinivasan N, Malini T, Arunakaran J, Aruldhas MM, and Govindarajulu

P (1998). Interactions of estradiol, progesterone and corticosterone on uterine connective

tissue degrading enzymes. Endocrine Res, 24: 89- 103.

19. Gladson M, Malini T, Arunakaran J, Govindarajulu P, and Srinivasan N (1998).

Interactions of estradiol, progesterone and corticosterone acetate in ovariectomized rats:

Effect of collagen and its fractions in uterus and cervix. J.Endocrinol.Reprod, 1: 92-103.

20. Gladson M, Anbu RB, Srinivasan N, and Govindarajulu P (1992). Biochemical

changes in rat testicular cells after vasectomy. Med.Sci.Res, 20: 187-189.

21. Valli G, Gladson M, Srinivasan N, and Govindarajulu P (1992). Influence of

corticosterone acetate on the spleen in intact and ovariectomized rats. Endocrinol. Jap,

39: 549- 555.

BOOK CHAPTER:

1. Kumar A, Gladson M, Tyagi K, and Ahamad, N. (2000). The phytoestrogen of promise:

shokarishta. Recent Research in Gynecological Endocrinology (Book) p 169 -172. Edited

by AR Genazzani, PG artini and F. Petraglia, The Parthenon publishing Group, New York,

and London.

ARTICLES UNDER PREPARATION

1. Muthian G, Charlton CG. Prenatal MPTP may cause the inhibition of uterine progesterone

that leads to depletion of tyrosine hydroxylase and Dopamine in sensitization and

Precipitation stage for Parkinson's disease model in C57bl/6J female mice.

( Reproduction and fertility )

2. Gladson Muthian, Marquita Smith, Jennifer King, Brenya Griffin, Veronica Mackey

Charlton Clivel. Curcumin blocked the proposed precipitating stage and sensitization stage

for Parkinson’s disease: A potential preventative and ameliorating agent for PD. (PLOS one

– Submitted)

3. Gladson Muthian , Kedar Prasad and Charlton Clivel. Blockage of an MPTP model of

Parkinson’s disease by Antioxidants: A potential Preventative Measure for PD: “Progress

and Promise” (PLOS one)

4. Brenya Griffin, Gladson Muthian, Marquita Smith, Veronica Mackey, Clivel Charlton.

Methylation and the sensitization- precipitation Hypthesis of Parkinson’s disease.

(Biochimica et Biophysica Acta (BBA) Molecular Basis of disease.

5. Muthian G, King. J,Dent L, Mackey V, Smith M, and Charlton CG. Prenatal and Postnatal

diagnosis of fetal striatal cytoskeleton protein changes after maternal MPTP exposure

during pregnancy in C57BL/6J mice.

6. Muthian et al. Binary effects of L- methionine (MET) in causing Parkinson’s disease like

behavioral and molecular changes in C57BL mice sensitized with prenatal MPTP

7. Muthian et al. Co-operation between prenatal MPTP and postnatal SAM in the reduction of

striatal dopamine, DOPAC and 3MT in C57BL mice.

ARTICLES PENDING

1. Muthian G and Bright JJ. PPAR γ agonists inhibit IFNγ -induced activation of JAK-STAT

pathway in microglia.

2. Muthian G, Rajasingh J, Raikwar HP, and Bright JJ. Histamine receptor antagonists

modulate Th1 response in EAE by blocking IL-12 signaling through JAK-STAT Pathway in

T- Lymphocytes

GRANTS AWARDED:

Dabur Research Foundation, Dabur India Limited.

“Investigating the effect of Ashokarishta in uterine collagen metabolism in mice”.

Papers presented in Conference:

1. Muthian G Smith M, Griffin B, Mackey V and Charlton (2012) Co-operation between

prenatal MPTP and postnatal SAM in the reduction of striatal dopamine, DOPAC and

3MT in C57BL mice. (SFN meeting, New orleans 2012).

2. Muthian G, Mackey VR, Dent L, Smith M, King C, Charlton CG (2011) Peripheral

adenosine triphosphate (ATP) and L-methionine (Met) caused Parkinson’s disease-like

behavioral and molecular changes in C57BL mouse exposed to prenatal and postnatal to

MPTP.( SFN meeting, Washington DC).

3. Muthian G, King J, Dent L, Smith M, Mackey V and Charlton CG (2010). Blockage of the

proposed precipitating and sensitization stage for Parkinson’s disease by curcumin: a

potential preventive measure for PD. Society for Neuroscience, San Diego,

4. Muthian G, King J, Mackey V and Charlton CG (2009). The reduction in cytoskeleton

proteins may underlie the sensitization of basal ganglia in Parkinson’s disease. SFN,

Chicago.

5. Muthian G, Mackey V, King J, Charlton CG (2009). Prenatal and Postnatal depletion of

(LAAD) by MPTP as a sensitization and Precipitation Model for Parkinson’s disease.

Experimental Biology, New Orleans.

6. Muthian G, King J, Mackey V and Charlton CG (2008). Prenatal and Postnatal depletion

of dopamine and its metabolites by 1-methyl-1,2,3,6 tetrahydropyridine as a sensitization

and precipitation “ model for Parkinson’s disease. SFN Washington DC,

7. Muthian G, Mackey V, King J, Charlton CG (2008).Prenatal and postnatal depletion of

Tyrosine hydroxylase by MPTP as a sensitization and precipitation model for Parkinson’s

disease. Experimental Biology San Diego.

8. Muthian G, King J, Mackey V, Prasad K and Charlton CG (2008). Blockage of the

proposed precipitating stage for Parkinson’s disease by antioxidants: A potential

preventive measure for PD. Experimental Biology, San Diego.

9. John J. Bright, Himanshu P. Raikwar and Gladson Muthian (2007). Curcumin

induces growth arrest and appptosis by blocking constitutively active JAK-3 –STAT5

signaling pathway in HTLV-1 transformed T cell leukemia. Abstract # 5192 (AACR)

10. Rajasingh J, Muthian G, Raikwar HP and Bright JJ (2005).Nuclear receptor

agonists regulate LIF signaling through JAK-STAT pathway in Embryonic Stem

Cells.Abstract # 555.13 .Participated and presented abstract in the Annual

Meeting of the American Association of Immunology, San Diego, California, USA.

March 31 – April 6.

11. Muthian G, Raikwar HP. Rajasingh J, Johnson C and Bright JJ (2005). PPAR γ

agonists inhibit IFNγ -induced activation of JAK-STAT pathway in microglia. Abstract #

562. 3 Participated and presented abstract in the Annual Meeting of the American

Association of Immunology, San Diego, California, USA.March 31- April 6.

12. John J.Bright, Himanshu P. Raikwar, Gladson Muthian (2004). PPAR gamma

antagonist, bisphenol A diglycidyl, exacerbates neural antigen- induced Th1 response

and experimental allergic encephalomyelitis Abstract # 780. 13 (An Annual meeting of

professional Research Scientists, Experimental Biology, Washington DC, USA. April 17

– 21.

13. Bright JJ, Natarajan C and Muthian G (2003). PPRγ is a physiological regulator of Th1

cell mediated autoimmune diseases. Abstract # 126.6.Participated and presented

abstract in the Annual Meeting of the American Association of Immunologists, held at

Denver, Colorado, USA. May -6-10.

14. Muthian G and Bright JJ (2003). Quercetin ameliorates EAE by blocking IL -12

signaling through JAK-STAT pathway in T lymphocytes. Abstract # 124.39. Participated

and presented abstract in the Annual Meeting of the American Association of

Immunologists, held at Denver, Colorado, USA. May 6 -10.

15. John J.Bright, Himanshu P. Raikwar, Gladson Muthian (2004). PPAR gamma

antagonist, bisphenol a diglycidyl, exacerbates neural antigen- induced Th1 response

and experimental allergic encephalomyelitis Abstract # 780.13. (An Annual meeting of

professional Research Scientists, Experimental Biology, Washington DC, USA. April 17-

21.

16. A.Kumar, M. Gladson, K.Tyagi and N. Ahmad (2000).The phytoestrogen of promise:

Ashokarishta. Participated and presented abstract in the 8th World Congress of

Gynecological Endocrinology, Florence, Italy December 6-9.

17. Gladson M, Malini T, Srinivasan N and Govindarajulu P (1999). Interactions of ovarian

hormones and adrenal hormone on uterine glycosaminoglycans in rats.

Participated and presented research paper in International symposium on molecular

and Clinical aspects of the regulation of ovarian function impact on the Reproductive

Health of women, held at All India Institute of Medical Sciences, (AIIMS)India. October

6-8.

.

18. Gladson M. Rajkumar L, L. Srinivasan and Govindrajulu (1994) Hormonal influence on

uterine collagen in mature albino rats. Participated and presented research paper in

satellite symposium on collagen structure, Biology and Technology. Central Leather

Research Institute, Chennai, India. September 15 -17.

19. Gladson M, Srinivasan N, Arunakaran J, Muthu Mohamad HS and Govindarajulu P.

(1990). Changes in glycolytic and ATP-ase activities in rat epididymis after vasectomy.

Abstract # 54. All India Symposium on Reproductive Biology and General

Endocrinology, Dept. of Zoology, MS University of Baroda, Baroda, India. Jan 8-11.

20. M.Gladson, HS. Muthu Mohamed, N.Srinivasan, J.Arunakaran and P.

Govindarajulu (1991). Role of estradiol on uterus and cervix of mature bonnet

monkeys, Macaca radiata. Abstract # RE 28.IX All India Symposium on Reproductive

Biology and Comparative Endocrinology, School of Life Sciences, Bharathidasan

University, Tiruchirapalli, India. Feb – 20 -22.

21. Varalakshmi Mrinalini G, Valli G, Gladson M, Srinivasan N and GovindarajuluP.

Impact of corticosterone acetate on renal lipids in intact and ovariectomized rats.

(1992).Abstract # VE61. X All India Symposium on Reproductive Biology and

Comparative Endocrinology, Avinashingam Institute for home science and higher

education for women (Deemed University), Coimbatore-641 043. India. Jan 12 & 13.

22. Gladson M, Srinivasan N, Rajkumar L, Valli G and Govindarajulu P. Interaction of sex

steroids and corticosterone on uterine collagen in young adult albino rats.

(1993).Abstract # 125. XI National Symposium on Reproductive Biology and Jan 29-

30General Endocrinology, Dept. of Zoology, Sri Venkateswara University, Tirupati-517

502, AP, India. Jan 29-30.

23. Sunil N, Gladson M, Arunakaran J, Srinivasan N and Govindarajulu P. Influence of

adrenalectomy on renal lipids in intact and ovariectomized rats. (1993). Abstract # 126.

XI National Symposium on Reproductive Biology and General Endocrinology, Dept. of

Zoology, Sri Venkateswara University, Tirupati-517 502, AP. India. Jan 29-30,

24. Udayakumar IS, Gladson M, Arunakaran J, Srinivasan N and Govindarajulu P.

Influence of adrenalectomy on lipids in selected regions of brain of intact and

ovariectomized rats. Abstract # 127. XI National Symposium on Reproductive Biology

and General Endocrinology, Jan 29-30, 1993. Dept. of Zoology, Sri Venkateswara

University, Tirupati-517 502, AP. India.

25. Sunil N, Gladson M, Srinivasan N, Arunakaran J, Aruldhas MM and Govindarajulu P.

Glycosaminoglycan concentration in mammary gland of normal cyclic Tamoxifen treated

and DMBA induced mammary tumours of Sprague Dawley rats.( 1996). Abstract #

21 .XIV National Symposium on Reproductive Biology and General Endocrinology,

Dept. of Zoology, Gujarat University, Ahmedabad-380 009. India. Jan 25-27.

Workshop / Symposium/Conference Participated:

1. Participated Indo German workshops on Fertilizations: Lessons from In vitro

approaches, October9 -10, 1999.

2. Participated in silver jubilee International Conference on molecular and Metabolic

Endocrinology and contraceptive Technology. October 21 22, 1994.Participated in the

seminar on Recent Trends in Endocrinology and Reproduction, March 5 -6, 1993.

REFERENCES:

1. Dr. Charlton Clivel George, Ph.D. (current advisor)

Professor and Chairman

Dept. of Neuroscience and Pharmacology

1005 Dr. D B Todd Jr Blvd

Meharry Medical College,

Nashville, TN 37208.

Tel: 615-***-****

Fax: 615-327- 6632Email: *********@***.***

2. Dr. Bruner-Tran, Ph.D.

Associate Professor of Women’s Reproductive Health Research Center

B-1100 MCN, 2519

Vanderbilt University Medical School

Nashville, TN 37212

Tel: 615-***-**** Email: ******.******-****@**********.***

3. Dr. Leon Dent MD, Ph.D.

Professor and Chair

Dept. Of Surgery, Meharry Medical College,

Nashville, TN 37208

Tel: 327-6342

Email: *****@***.***

4.Dr. Anna Chockalingam DVM PhD CG(ASCP)

Clinical Research Scientist

Genetic Associates Inc

Nashville, TN 37211

Cell: 1-607-***-****

Email: ********@*****.***

Contact this candidate