*

CURRICULUM VITAE

*. Full Name (in capitals): (Dr.) E. KUMAR

Father’s name: Ebineshan

2.

Date of birth: 10th April 1987

3.

4. Nationality: Indian

6. Do you claim any age relaxation? No

7. Address for correspondence:

L.B.Puram (Village)

Nariga Palli (Post)

Chittoor

Chittoor (dist.) – 517 002. Andhra Pradesh, India.

Mobile No.:+91-709*-***-***; +91-812*-***-***

E-mail: acogs2@r.postjobfree.com

8 Permanent address:

L.B.Puram (Village)

Nariga Palli (Post)

Chittoor

Chittoor (dist.) – 517 002. Andhra Pradesh, India.

Mobile No.: +91-709*-***-***; +91-812*-***-***

E-mail: acogs2@r.postjobfree.com

2

9. Educational/Professional qualifications (SSC or 10th Class onwards)

S. Examination University or Subjects Year of Percentag Class/Divisio

No. passed Board taken passing e of n/Grade

marks

1. S.S.C State Science, 2002 62.66% First Class

Maths, Social

Studies

2. Intermediate State Botany 2004 68.4% First Class

Zoology

Chemistry

3. Bachelor Sri Biotechnolog 2007 65.11% First Class

of Science Venkateswara y

University Chemistry

Botany

4. Master Sri Microbiology 2009 67.63% First Class

of Science Venkateswara

University

(Campus)

5. Doctor of Sri Microbiology 2010 - 65.00% First Class

Philosophy Venkateswara (Medical) 2014

University

(Campus)

Note: Ph.D., Thesis submitted and waiting for Viva-Voice.

10. About Ph.D., thesis work

The Doctoral Thesis work deals with detection of emerging beta lactamase mediated

antibiotic resistance mechanisms in the clinical isolates of Acinetobacter spp. including

Extended spectrum beta lactamases (ESBLs), AmpC, Metallo beta lactamases (MBL), and

also the newer beta lactamases such as New Delhi metallo beta lactamase (NDM).

The current CLSI (Clinical and Laboratory Standards Institute) do not recommended

any phenotypic methods to detect ESBL mediated resistance mechanisms in Acinetobacter

spp. However, this method only recommended for E.coli and Klebsiella spp. In our

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objectives, the CLSI recommended method was modified and applied for the detection of

ESBL detection in Acinetobacter spp.

Development of Multiplex PCR for the detection of ESBL mediated resistance genes

such as CTX-M, SHV, TEM and detection of genes which are responsible for the AmpC,

MBL, and NDM mediated antibiotic resistance mechanisms. Characterization of beta

lactamases antibiotic resistance genes. Finally correlate the phenotypic and genotypic

detection methods.

The clinical features were obtained from patients those infected with Acinetobacter

spp. and were significance was verified between beta lactamase producers and non-

producers.

Additionally, the biofilm formation and plasmid profiling and curing were performed

to see the significance with beta lactamase medicated resistance mechanisms

11. Any other relevant information you wish to add such as research papers published,

Conferences, training courses attended, etc. (Use separate sheets if necessary)

i. Project Work done:

“A Preliminary investigation Bacterial contamination in Ambulances handling

medical emergencies”, during Feb –march2009, Management and Research Institute

(EMRI) and Department of Virology, S. V. University.

ii. Computer Knowledge:

Basic Aspects of Operating systems like MS Windows, Linux, Irix, Suites like

MSoffice, Networking and also many of the bioinformatics tools and Software suites

related to Sequence analysis.

iii. Hands on training and Workshops

1. Participated in National workshop on “Genomics, Proteomics and Bioinformatics” at

Department of Biochemistry & Biotechnology, Annamalai University – 608 002,

Tamilnadu during 19th to 21st July, 2013.

2. Participated in “National workshop on Bioinformatics” held at Bioinformatics

Centre and ARIS cell, Madras Veterinary College, Chennai- 600 007, during 20-21th

Nov, 2012,.

3. Short term course on “Data analysis using software” held at University Grants

Commission, Academic Staff College, Maduri Kamaraj Universit y, Madurai, during

15-20th Oct 2012.

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4. Workshop on “Dark Field Microscopy for Leptospirosis” held at Sri Venkateswara

Institute of Medical Sciences (SVIMS), Tirupati on 5th Feb, 2011.

5. XXIV Winter School on “Mutagenesis: A powerful tool in Bacterial system” during

4th- 18th July 2012, held at UGC-Networking Resource Centre in Biological Sciences,

School of Biological Sciences, Madurai Kamaraj University, Madurai.

6. Hands on training program on “Ribosomal DNA (rDNA) based microbial

identification technique” organized by the Department of Biotechnology, Karpagam

University, Coiambatore, Tamil Nadu, India during 5-7th Jan 2012.

7. Participated in National workshop on “Advances in Modern Biology” held at Watson

Life Sciences, Tirupati on 3rd to 5th August, 2010.

iv. National Seminars and Conferences Participated

1. Participated in “Open House and Exhibition” held during 23rd to 27th August, 2007,

Sri Venkateswara University, Tirupati.

2. Participated in National conference on “Recent trends in viral disease problems and

management (VIROCON-2010)” during 18th – 20th March, 2010 held at Sri

Venkateswara University, Tirupati.

3. Participated in “2nd A.P. Science Congress” organised by Andhra Pradesh Akademi

of Sciences, Hyderabad and Sri Venkateswara University, Tirupati, on Nov 14 -16,

2009.

4. Participated in National Seminar on “Vedic Science in relevance to Modern Science”

held at Sri Venkateswara University, Tirupati on September 18, 2010.

5. Participated in National seminar on “Biotic and Abiotic Molecules – Their Health

Effects and Therapeutic Applications (BAMHT-2012)” held at Sri Venkateswara

University, Tirupati on 24th & 25th February, 2012.

6. Participated and presented Oral presentation in “National traditional Seed Mela

Conference” conducted by South Asia Rural Association (SARAA), Bangalore and

Department of Virology, Sri Venkateswara University, Tirupati, on 23rd June, 2012.

7. Participated in International conference on “Environmental impact on human health

and therapeutic challenges (ICEHT-2012)” conducted by Association of

Biotechnology and Pharmacy( ABAP) and Department of Virology, Sri Venkateswara

University, Tirupati 20th -22nd Dec 2012.

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8. Participated in EAC on Entrepreneurship development Cell conducted by Dept. of

Management Studies, Sri Venkateswara University, Tirupati and DST-PURSE

programme, Sri Venkateswara University, Tirupati during 11th – 12th Nov 2013.

v. Publications in Journals (Aggregate Impact Factor: 9.985)

Published

1. E. Kumar, K. Usha, A. Chaudhury, BV. Ramana, DVR. Sai Gopal. Detection of

AmpC β- lactamases production in Acinetobacter species by an inhibitor (disc) based

& modified three dimensional (enzyme extraction) methods. Indian Journal of

Medical Research 2014; 140: 688-90. (Impact Factor- 1.661).

2. E. Kumar, K.Usha, BV. Ramana, A. Chaudhury, DVR. Sai Gopal. Prevalence of

Acinetobacter species and its antibiogram pattern in a tertiary care hospital, Tirupati,

Andhra Pradesh. Inter J Pharm. Res. BioSci. 2014; Volume 3(1): 352-358. (Impact

factor: 1.862)

3. K. Usha, E. Kumar, Usha Kalawat, B. Siddhartha Kumar, A. Chaudhury, DVR. Sai

Gopal. Seroprevalence of Scrub typhus among febrile patients – a preliminary study

Asian J Pharm Clin Res. 2014; Vol 7, Suppl 1: 19-21. (impact factor - 0.7)

4. E. Kumar, K. Usha, BV. Ramana, A. Chaudhury, DVR. Sai Gopal. Prevalence of

various β-lactamase (ESBL, AmpC and MBL) producing multidrug resistant clinical

isolates of Acinetobacter spp. in a tertiary care hospital. Asian J Pharm Clin Res,

vol6, suppl 4, 2013. 28-31.(impact factor - 0.7)

5. K.Usha, E. Kumar, DVR. Sai Gopal. Occurrence of various beta-lactamase

producing Gram negative bacilli in the hospital effluent. Asian J Pharm Clin Res,

Vol 6, Suppl 3, 2013, 42-46. (impact factor - 0.7)

Accepted (Press)

6. E. Kumar, K. Usha, A. Chaudhury, BV. Ramana, DVR. Sai Gopal. High occurrence

of ESBL combination genes (blaCTX-M and blaSHV) among Acinetobacter spp. in a

South Indian tertiary care hospital. Indian Journal of Medical Research. (Impact

Factor- 1.661).

7. E. Kumar, K. Usha, A. Chaudhury, BV. Ramana, DVR. Sai Gopal. Molecular

characterization of plasmid mediated blaCTX-M15 Extended spectrum beta lactamase

(ESBLs) in Acinetobacter spp. isolates from ICU patients, at a tertiary care hospital,

South India. Indian Journal of Medical Microbiology. (Impact Factor 1.04)

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8. K. Usha, E. Kumar, Usha Kalawat, A. Chaudhury, B. Siddhartha Kumar, DVR. Sai

Gopal. Molecular detection of clinically relevant Orientia tsutsugamushi serotypes

causing outbreak in Southern Andhra Pradesh, India. Indian Journal of Medical

Research (Impact Factor - 1.661)

vi. NCBI Accession number assigned:

i. KJ083256: Kumar, E., Usha,K., Ramana,B.V., Chaudhury,A. and Sai Gopal,

D.V.R. Acinetobacter sp. SVU/SVIMS1 plasmid extended-spectrum beta-

lactamase SHV-12 (blaSHV-12) gene, partial cds.

ii. KJ127475: Kumar, E., Usha,K., Ramana,B.V., Chaudhury,A. and Sai

Gopal,D.V.R.Acinetobacter sp. SVU/SVIMS1 beta lactamase CTX-M-15

(blaCTX-M-15) gene, partial cds.

iii. KJ127476: Kumar, E., Usha,K., Ramana,B.V., Chaudhury,A. and Sai

Gopal,D.V.R.Acinetobacter sp. SVU/SVIMS10 beta-lactamase TEM

(blaTEM) gene, partial cds.

iv. KJ094995: Usha,K., Kumar,E., Kalawat,U., Siddhartha Kumar,B.,

Chaudhury,A. and Sai Gopal,D.V.R. Orientia tsutsugamushi clone

SVU/SVIMS 28 56kDa type-specific antigen gene, partial cds.

v. KJ094996: Usha,K., Kumar,E., Kalawat,U., Siddhartha Kumar,B.,

Chaudhury,A. and Sai Gopal,D.V.R. Orientia tsutsugamushi clone

SVU/SVIMS 50 56kDa type-specific antigen gene, partial cds.

vi. KJ094997: Usha,K., Kumar,E., Kalawat,U., Siddhartha Kumar,B.,

Chaudhury,A. and Sai Gopal,D.V.R.Orientia tsutsugamushi clone

SVU/SVIMS 52 56kDa type-specific antigen gene, partial cds.

vii. KJ740606: Usha,K., Kumar,E., Kalawat,U., Siddhartha Kumar,B.,

Chaudhury,A. and Sai Gopal,D.V.R.Orientia tsutsugamushi clone

SVU/SVIMS CTR 56 kDa type specific antigen gene, partial cds.

viii. KJ740607: Usha,K., Kumar,E., Kalawat,U., Siddhartha Kumar,B.,

Chaudhury,A. and Sai Gopal,D.V.R. Orientia tsutsugamushi clone

SVU/SVIMS ANTPR 56 kDa type specific antigen gene, partial cds.

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vii. Acted as Resource Person for workshop on molecular diagnosis of parasitic

infections.

(1st Andhra Pradesh State Chapter Conference of Indian Academy of Tropical

Parasitology (IATP) Organised by Department of Microbiology, Sri Venkateswara

Institute of Medical Sciences (SVIMS), Sri Padmavathi Medical College for Woman,

Tirupati during 19-20 December 2014.

12. Techniques known

Bacterial Identification

Antibiotic sensitivity tests by disc diffusion and agar dilution method

Detection of beta lactamase producers among bacterial species

Different methods of Genomic and Plasmid DNA isolation from various sources.

Different types of PCR-Conventional PCR, Multiplex PCR, Nested PCR and Real

Time PCR.

Sequence analysis

Use of Bioinformatics tools

Use of various Statistical tools including SPSS and Excel.

13. References:

Dr. DVR. Sai Gopal Dr. Abhijith Chaudhury, Dr. B.V. Ramana,

Professor and Head, Professor & Head, Assistant Professor

Department of Virology, Department of Microbiology, Department of Microbiology,

Sri Venkateswara University Sri Venkateswara Institute of Sri Venkateswara Institute of Medical

(SVU), Medical Sciences (SVIMS) Sciences (SVIMS)

Tirupati-517502, & &

Phone : +91-984******* Sri Padmavathi Medical College for Sri Padmavathi Medical College for

Email: Woman Woman

acogs2@r.postjobfree.com Tirupati, Andhra Pradesh. Tirupati, Andhra Pradesh.

Phone: +91-988******* Phone no: 094**-***-***.

Email: Email:

acogs2@r.postjobfree.com

acogs2@r.postjobfree.com

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Declaration

I affirm that the information given in this application is true and correct to the best of my

knowledge and belief. I also fully understand that if at any stage it is discovered that any

attempt has been made by me to wilfully conceal or misrepresent the facts, my candidatu re

may be summarily be rejected.

Place: Tirupati

Date: 19/01/2015 Signature of the candidate

Contact this candidate