Project Assistant

Arti Sharma

Current Appointment:

Home Address:

Clinical Laboratory Scientist

*** ********* ****

NeoDiagnostix Inc, # 247 Gaithersburg

**** ***** ****** ***

MD-20878

Rockville, MD - 20850

Telephone number: 615-***-****

Email: *********@*****.***

Education

Master of Science

Aug 2007 - Dec 2008

Major: Biotechnology GPA: 3.71/4

Florida Institute of Technology, Florida

Master of Science

Aug 2004 - Jun 2006

Major: Biotechnology GPA: 3.87/4

Amity University, India

Bachelor of Science

Jul 2001 - Jun 2004

Major : Environment Science GPA: 3.37/4

University of Delhi, India

Certification and Licenses

Clinical Laboratory Specialist in Cytogenetics - CG(ASCP) - certified

by National Credentialing Agency for Laboratory Personnel. Certificate

number 9917076.

Medical Laboratory Professional - Licensed by the State of Tennessee

(TN) Division of Health Related Boards. License Number ML22702.

Special Analyst - Cytogenetics Training program from Genetics Associates,

Inc. Nashville, TN.

Work Experience

NeoDiagnostix Inc., MD

May 2013 - Present

Clinical Laboratory Scientist :.

Perform Cervical DNA Dtex Test - Its a Fluorescence In Situ

Hybridization (FISH) assay that utilizes a novel biomarker to

identify specific chromosomal abnormalities in cervical cells that

have been linked to cervical cancer.Amplification of chromosome region

3q26 indicates an elevated risk for development of severe

precancerous lesions and cancer.

Assist Laboratory Director and Medical Director in operation of

clinical diagnostic laboratory.

Assist in developing and establishing policies and procedures for the

laboratory.

Performs and interprets results of clinical laboratory tests

Develops, improves and validates molecular diagnostic tests

Contribute to ongoing R&D project

Contribute to the development of scientific manuscripts and data

presentation

Genetic Associates Inc., Nashville, TN

Jun 2008 - May 2013

Cytogenetic Skills :

Microscopic analysis of bone marrow and blood for cancer diagnosis,

identification of chromosomal abnormalities found in Leukemia's,

Lymphoma's, Myeloproliferative Neoplasms, Myelodysplastic Syndromes,

solid tumors etc.

Set-up of blood,bone marrow. Isolate, identify and prepare specimens

for examinations, Proficient in sterile tissue culture techniques,

harvesting and chromosome spreading, banding .

Role in prenatal studies

Set-up/Culture maintenance/Evaluation of prenatal samples:

Amniotic Fluid- Set up cover-slips and flasks.

Chorionic Villi -Obtaint CVS from clinics after identifying under

microscope, cleaning the sample, separating villi from maternal tissue.

Products of conception - specimen cleaning and separate the villi or fetal

parts from other tissue.

Feeding and flooding of Cover-slips and Flasks.

Harvest/slide making: Cover-slip harvesting, direct harvesting of

Chorionic Villi and Flask harvesting of products of conception

samples.

Microscopic analysis of prenatal samples.

Operate Cytovision software of imaging system for Karyotyping and

Providing detailed analysis .

Chromosome analysis by Fluorescence In Situ Hybridization (FISH).

Research work : Identification of gaps and breaks caused by smoke chemicals

on fetal cells.

Molecular Diagnostics and Molecular Cytogenetic skills :

.

Working in the implementation of PCR laboratory and write protocols.

Extensive hands-on experience on DNA / RNA extraction from various

specimens types, Running RT-PCR and Real -Time PCR. Working on Test

Validations for JAK2 and BCR/ABL Instruments used are Rotor Gene-Q

(Qiagen), Thermal Cycler (Applied Biosystem), NanoDrop

spectrophotometer.

Assistance with the primary validation of an oligo-comparative genomic

hybridization (aCGH) array chip which includes mastery of the

technical protocol and comparison of the new technology with the

current bacterial artificial chromosome (BAC) aCGH array. Duties

include collection and organization of specimens for comparative

study, data collection, organization and presentation.

Florida Institute of Technology, Melbourne, FL

Aug 2007 - Apr 2008

Research Assistant

Role in the project: Understanding molecular mechanisms that regulate

bacterial growth. Techniques used include PCR, RT-PCR, Flow cytometry,

Spectrophotometer to gain knowledge about the growth patterns of various

bacteria.

Ashoka Trust for Research in Ecology and Environment (ATREE),

New Delhi, India

Feb 2007 - Aug 2007

Program Associate

Role: Handled various conservation education programs and technical

workshops.

Micro Labs Ltd., India

Mar 2006 - May 2006

Intern

Project Title: Formulation and Analysis of Mirtazapine Tablets 15 mg

Role in the Project: Performed formulation of drug Mirtazapine 15 mg and

its chemical analysis by HPLC, Gas Chromatography and Microbiology test.

Mirtazapine is a prescription antidepressant drug, used primarily for the

treatment of clinical depression in adults. It is considered as a second-

line or even third-line antidepressant, to be used when older

antidepressants are not tolerated or are ineffective.

Indian Institute of Technology, India

Feb 2006 - Mar 2006

Intern

Project Title: Fluorescent Pseudomonads as a PGPR

Performed experiments of Media Optimization for maximum growth of

Fluorescent Pseudomonads and learnt various microbiological and biochemical

techniques related to fermentation.

Mohan Meakin Limited (Brewery), India

Jun 2005 - Jul 2005

Intern

Worked on fermentation and biochemical analysis of various alcohols.

St. Stephen's Hospital, India

Jul 2005 - Aug 2005

Intern

Project Title: Diagnostic Tests for microbial diseases

Implementation of microbiology tests: Performed Gram staining of

Bacteria, Fungal Staining, Albert Stain, AFB Stain, Coagulase Test,

Optochine sensitivity Test, Bacitracin Test, Hanging Drop Preparation.

Additional Skills and Activities

Computer Proficiency: MS-Office, Database Management.

Attended the Association of Genetic Technologists, Inc. (AGT)

meeting 2012 in

Atlanta,USA.

Attended a scientific conference "BIOHORIZON" 2006 and seminar on

Environmental

issues at IIT, Delhi.

Active student member of the Nature club of Maitreyi College, Delhi

University.

Actively participated in various activities like paintings, plays and

athletics at school and

college levels.

References: On request

Contact this candidate