Management Assistant
Resume:
Arindam Bose
UConn **N Eagleville Road, Storrs, CT 06269 860-***-**** *******.****@*****.***
www.linkedin.com/in/orindambose/
Education
Ph.D., Chemistry, University of Connecticut, Storrs, CT Feb 2015
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Area of Concentration: Biological Chemistry
Dissertation Topic: Mechanism of Translesion Synthesis of mutagenic DNA lesions in wild type
and specific polymerase knockdown human cells.
M.S., Organic Chemistry, University of Delhi, Delhi, India July 2005
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B.S., Chemistry (Honors), University of Calcutta, Kolkata, India July 2003
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Skills
Cancer genetics, DNA toxicology, chemical carcinogenesis, DNA damage and repair.
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Recombinant DNA technology, molecular cloning, transformation, transfection, competent cell
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preparation, site-directed mutagenesis, enzyme kinetics assay, cytotoxicity assay.
Hands on research expertise in mammalian cell culture, cryopreservation, preparation of
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mammalian cells lacking DNA polymerase(s) by specific gene knockdown using siRNA.
In depth knowledge in extraction, purification and characterization of DNA, RNA and proteins
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from bacterial & mammalian cells, strategic designing of synthetic oligonucleotides and primers.
DNA sequencing, genome engineering, oligonucleotide hybridization, PCR, RT-PCR, Q-PCR,
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Western blot, SDS-PAGE, ELISA and Flow Cytometry. Purification and characterization of
oligonucleotides using HPLC, PAGE, LC-MS, NMR and UV-VIS.
Dexterous in troubleshooting, document preparation/review, literature search, timeline
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management, organization, team work, technology transfer, safety evaluation and approval,
regulated waste management including biological and radiological.
Ability to succeed at multitasking projects and communicate across technical boundaries.
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Positions
Graduate Teaching Assistant
Department of Chemistry, University of Connecticut, Storrs, CT August 2008 – Present
Lecturer
Department of Chemistry, Career Point, Kota, Rajasthan, India August 2005 – July 2008
Research Intern
Synthesis and Characterization of mono-nuclear and di-nuclear copper (II) complexes of Polydentate
schiff base ligand with N, O donor atom.
Indian Institute of Technology, Kharagpur, India May 2004 – July 2004
Arindam Bose
Experience
Graduate Research, Department of Chemistry, University of Connecticut August 2008 – Present
Elucidated the mutagenic mechanism, cytotoxicity and DNA sequence context in the translesion
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synthesis of a major dietary carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), found in
cooked meats and fish, in human embryonic kidney cells.
Invented mutational analysis, genotoxicity and translesion synthesis of the environmental
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carcinogen 3-nitrobenzanthrone in human cells (HEK293) and simian kidney cells (Cos7).
Detected the mutational pattern of antitumor drug Mitomycin C in mammalian cell.
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Analyzed DNA repair of tumorigenic lesions formed due to γ radiation in bacterial cells.
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Developed, optimized, and validated specific gene knockdown (TLS DNA polymerases) in
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mammalian cells by RNA interference, expressed and purified engineered proteins.
Proficient in large scale preparation of low copy single stranded plasmid vectors and M13 phage
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vectors, purification, analysis and quantification, mini/maxi preparation, aseptic techniques.
Slick in native and denaturing gel electrophoresis including radiolabel based analysis and
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purification of normal and complex oligonucleotides, agarose gel and capillary electrophoresis.
Adroit in managing projects from concept to successful completions, analytical development,
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systematic presentation of research data to group members and collaborators.
Biological and Radiological (32P) waste management, monitoring laboratory biosafety, microbial
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decontamination, clean air device, maintaining BSL2 status according to OSHA, CDC and FDA
guidelines, radiological surveys, inventory control, personnel monitoring, risk assessments and
radiation safety training according to the Nuclear Regulatory Commission (U.S.NRC) guidelines.
Mentored graduate and undergraduate chemistry students in general and honors organic
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chemistry laboratory.
Honors and Awards
Doctoral Student Travel Award, University of Connecticut, July 2014.
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Doctoral Dissertation Fellowship, University of Connecticut, Research Foundation, May 2014.
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GATE fellowship for research, Government of India, March 2006.
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CSIR-UGC fellowship for research, Government of India, June 2005.
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D.C. Arora fellowship, University of Delhi, October 2004.
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Affiliations
American Chemical Society, Division of Chemical Toxicology
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Phi Lambda Epsilon, UConn Chemistry Chapter
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UConn Graduate Employee Union
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Arindam Bose
Publications
A. Bose, P. Pande, V. P. Jasti, E. K. Hawkins, C. J. Rizzo and A. K. Basu “ Translesion synthesis
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of the C8-deoxyguanosine adduct of the dietary mutagen 2-amino-3-methylimidazo[4,5-
f]quinoline (IQ) in the NarI sequence in human cells “ ( manuscript in preparation).
P. Pande, C. K. Malik, A. Bose, V. P. Jasti, and A. K. Basu “ Mutational Analyses of the C8-
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Guanine Adduct of the Environmental Carcinogen 3-Nitrobenzanthrone in Human Cells: The
Critical Roles of DNA Polymerase η, κ, and Rev1 in Error-Prone Translesion Synthesis “
Biochemistry 2014, 53, 5323 5331.
Presentations
Translesion synthesis of the C8-deoxyguanosine adduct of the dietary mutagen 2-amino-3-
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methylimidazo[4,5-f]quinoline (IQ) in the NarI sequence in human cells, American Chemical
Society, Division of Chemical Toxicology, San Francisco, CA, August 12, 2014.
Mutagenicity of C8-Guanine Adduct Formed by 3-Nitrobenzanthrone in Human Cells, 248 ACS
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National Meeting and Exposition, Sci-Mix, San Francisco, CA, August 11, 2014.
Mutagenicity of the Food Mutagen 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) Derived DNA
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Adducts in Human Cells, RT Major Symposium, Department of Chemistry, University of
Connecticut, Storrs, CT, October 3-4, 2012.
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