Assistant Medical
Resume:
CURRICULUM VITAE
Dr. Rehan Khan (Ph.D.)
Post-doctoral Fellow,
University of Manitoba,
Winnipeg, MB, CANADA
E-mail: *********.*****@*****.***
Educational Qualification:
Post-doctoral Fellow at University of Manitoba, Winnipeg, MB, Canada. (From 4
October 2013- 2 June 2014).
Ph.D. (Toxicology) from Hamdard University, New Delhi in 2013.
M.S. (Toxicology) from Hamdard University, New Delhi, in 2006 with 72.8%.
B.S. (Hons) Life Science from Aligarh Muslim University, Aligarh, in 2004 with
69.4%.
Awards:
Post-doctoral Fellow at Manitoba Institute of Cell Biology, Cancercare
Manitoba, University of Manitoba, Winnipeg, MB, CANADA.
Research Fellowship for Science and Meritorious Student (RFSMS) by
University Grants Commission (UGC), New Delhi, India.
Travel Grant awarded by Department of Science and Technology, Ministry of
Higher Education, New Delhi, India, for attending international conference
“The Tumor Microenvironment” held in Dublin, Ireland in September 2012.
Senior Research Fellowship (SRF) by Indian Council of Medical Research
(ICMR), New Delhi, India.
Award from Jamia Hamdard (Hamdard University) for publishing research
work in high impact (more than 3.0) journals “ PLoS One”, “British Journal of
Nutrition” and “Toxicology and Applied Pharmacology ”.
Memberships:
Member of Royal Microscopical Society
Member of European Association for Cancer Research (EACR)
Reviewer of the International Journals:
Toxicological Sciences; Toxicology Letters; Toxicology; BMC Complement ary
and Alternative Medicine; Food and Chemical Toxicology; Basic and Clinical
Pharmacology and Toxicology; Molecular and Cellular Biochemistry; Human
and Experimental Toxicology; Toxicology In-Vitro; Toxicology Mechanisms and
Methods; Journal of Pharmacy and Pharmacology; Cell Biochemistry and
Function.
Total Number of Publications: 27
List of Publications:
1. Khan R, Khan AQ, Lateef A, Rehman MU, Tahir M, Ali F, Hamiza OO, Sultana S.
Glycyrrhizic acid suppresses the development of precancerous lesions via regulating
the hyperproliferation, inflammation, angiogenesis and apoptosis in the colon of
Wistar rats. PLoS One. 2013;8(2):e56020. [PMID: 23457494] Impact Factor: 3.73.
2. Khan R, Khan AQ, Qamar W, Lateef A, Tahir M, Rehman MU Ali F, Sultana S.
Chrysin protects against cisplatin-induced colon toxicity via amelioration of oxidative
stress and apoptosis: probable role of p38MAPK and p53. Toxicology and Applied
Pharmacology, 2012 Feb 1; 258(3):315-29. [PMID: 22155348]. Impact Factor:
3.630.
3. Khan R, Abdul Quaiyoom Khan, Wajhul Qamar, Abdul Lateef, Farrah Ali, Muneeb
U. Rehman, Mir Tahir, Swati Sharma and Sarwat Sultana . Chrysin abrogates
cisplatin-induced oxidative stress, p53 expression, goblet cell disintegration and
apoptotic responses in the jejunum of Wistar rats. British Journal of Nutrition,
2012 Feb 6:1-12. [PMID: 22309980]. Impact Factor: 3.302.
4. Khan R, Sultana S. Farnesol attenuates 1,2-dimethylhydrazine induced oxidative
stress, inflammation and apoptotic responses in the colon of Wistar rats. Chemico-
Biological Interactions 2011 Jul 15;192(3):193-200. [PMID: 21453689]. Impact
Factor: 2.982.
5. Rehman MU, Tahir M, Quaiyoom A, Khan R, Lateef A, Oday-O-Hamiza, Hassan
SK, Ali N, Qamar W, Sultana S. Alleviation of hepatic injury by chrysin in cisplatin
administered rats: Probable role of inflammatory markers. Pharmacol Reports.
Impact factor: 2.165; March 2014 (accepted in press).
6. Khan AQ, Khan R, Tahir M, Lateef A, Rehman MU, Ali F, Hamiza OO, Hasan SK,
Sultana S. Silibinin inhibits tumor promotional triggers and tumorigenesis against
chemically induced two-stage skin carcinogenesis in Swiss albino mice: Possible
role of oxidative stress and inflammation. Nutrition and Cancer, 2013 Dec 23.
[Epub ahead of print]. [PMID: 24364787]. Impact Factor: 2.47.
7. Ali F, Khan R, Khan AQ, Lateef A, Tahir M, Sultana S. Assessment of augmented
immune surveillance and tumor cell death by cytoplasmic stabilization of p53 as a
chemopreventive strategy of three promising medicinal herbs in murine two stage
skin carcinogenesis. Integrative Cancer Therapies, 2013 Dec 19;13(4):351-367.
[PMID: 24363284]. Impact Factor: 2.354.
8. Rehman M, Tahir M, Khan A, Khan R, Hamiza O, Lateef A, Hassan S, Rashid S,
Ali N, Zeeshan M, Sultana S. D-limonene suppresses Doxorubicin induced oxidative
stress and inflammation via repression of COX-2, iNOS, and NFκB in kidneys of
Wistar rats. Experimental Biology and Medicine (Maywood) 2014 Apr;239(4):465-
76. [PMID: 24586096]. Impact Factor: 3.103.
9. Hamiza OO, Rehman MU, Khan R, Tahir M, Khan AQ, Lateef A, Sultana S.
Chemopreventive effects of Aloin against DMH-induced preneoplastic lesions in the
colon of Wistar rats. Human and Experimental Toxicology, Accepted 2013. [PMID:
23928829]. Impact Factor: 1.453.
10. Tahir M, Rehman MU, Lateef A, Khan AQ, Khan R, Qamar W, Hamiza OO, Ali F,
Hasan SK, Sultana S. Diosmin abrogates chemically induced hepatocarcinogenesis
via alleviation of oxidative stress, hyperproliferative and inflammatory markers in
murine model. Toxicology Letters, 2013; 220(3):205-18. [PMID: 23665045]. Impact
Factor: 3.355.
11. Khan AQ, Khan R, Qamar W, Lateef A, Rehman MU, Tahir M, Ali F, Hamiza OO,
Hasan SK, Sultana S. Geraniol attenuates 12-O-tetradecanoylphorbol-13-acetate
(TPA)-induced oxidative stress and inflammation in mouse skin: possible role of p38
MAP Kinase and NF-κB. Experimental and Molecular Pathology 2013 Feb
8;94(3):419-429. [PMID: 23399806]. Impact Factor: 2.881.
12. Rehman MU, Tahir M, Khan AQ, Khan R, Lateef A, Oday-O-Hamiza, Qamar W,
Ali F, Sultana S. Chrysin suppresses renal carcinogenesis via amelioration of
hyperproliferation, oxidative stress and inflammation: plausible role of NF-κB.
Toxicology Letters 2013 Feb 4;216(2-3):146-58. [PMID: 23194824]. Impact
Factor: 3.355.
13. Khan AQ, Khan R, Rehman MU, Lateef A, Tahir M, Ali F, Sultana S. Soy
isoflavones (daidzein & genistein) inhibit 12-O-tetradecanoylphorbol-13-acetate
(TPA)-induced cutaneous inflammation via modulation of COX-2 and NF-κB in Swiss
albino mice. Toxicology. 2012 Dec 16;302(2-3):266-74. [PMID: 22981962]. Impact
Factor: 3.745.
14. Rehman MU, Tahir M, Khan AQ, Khan R, Lateef A, Oday-O-Hamiza, Ali F,
Sultana S. Diosmin protects against TCE (trichloroethylene) induced renal injury in
Wistar rats: Plausible role of p53, Bax and caspases. British Journal of Nutrition,
2013 Feb 12:1-12. [PMID: 23402272]. Impact Factor: 3.302.
15. Lateef A, Khan AQ, Tahir M, Khan R, Rehman MU, Ali F, Hamiza OO, Sultana S.
Androgen deprivation by flutamide modulates uPAR, MMP-9 expressions, lipid
profile and oxidative stress: amelioration by daidzein. Molecular and Cellular
Biochemistry, 2013 Feb;374(1-2):49-59. [PMID: 23135684]. Impact Factor: 2.388.
16. Tahir M, Rehman MU, Lateef A, Khan R, Khan AQ, Ali F, Oday-O-Hamiza,
Sultana S. Diosmin protects against ethanol-induced hepatic injury via alleviation of
inflammation and regulation of TNF-α and NF-κB activation. Alcohol, 2013
Mar;47(2):131-9. [PMID: 23419394]. Impact Factor: 2.038.
17. Hamiza OO, Rehman MU, Tahir M, Khan R, Khan AQ, Lateef A, Ali F, Sultana S .
Amelioration of 1, 2 Dimethylhydrazine (DMH) induced colon oxidative stress,
inflammation and tumor promotion response by Tannic acid in Wistar rats. Asia
Pacific Journal of Cancer Prevention, 2012;13(9):4393-402. [PMID: 23167349].
Impact Factor: 1.5.
18. Qamar W, Khan AQ, Khan R, Rehman MU, Lateef A, Tahir M, Ali F, Sultana S.
Benzo(a)pyrene-Induced Pulmonary Inflammation, Edema, Surfactant Dysfunction,
and Injuries in RATS: Alleviation by Farnesol. Experimental Lung Research. 2012
Feb;38(1):19-27. [Epub ahead of print] [PMID: 22168545]. Impact Factor: 1.754.
19. Qamar W, Khan R, Khan AQ, Rehman MU, Lateef A, Tahir M, Ali F, Sultana S.
Alleviation of lung injury by glycyrrhizic acid in benzo(a)pyrene exposed rats:
Probable role of soluble epoxide hydrolase and thioredoxin reductase. Toxicology
2012 Jan 27;291(1-3):25-31. [Epub ahead of print] [PMID: 22051199]. Impact
Factor: 3.745.
20. Khan AQ, Khan R, Qamar W, Lateef A, Ali F, Tahir M, Muneeb-U-Rehman,
Sultana S. Caffeic acid attenuates 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-
induced NF-κB and COX-2 expression in mouse skin: Abrogation of oxidative stress,
inflammatory responses and proinflammatory cytokine production. Food and
Chemical Toxicology. 2012 Feb;50(2):175-83. [PMID: 22036979]. Impact Factor:
2.610.
21. Sarwat Sultana, Verma K, Khan R. Nephroprotective efficacy of chrysin against
cisplatin induced toxicity via attenuation of oxidative stress. Journal of Pharmacy
and Pharmacology, 2012 Jun;64(6):872-81. [PMID: 22571266]. Impact Factor:
2.033; Citation: 14.
22. Rehman MU, Tahir M, Ali F, Qamar W, Lateef A, Khan R, Quaiyoom A, Oday-O-
Hamiza, Sultana S. Cyclophosphamide-induced nephrotoxicity, genotoxicity, and
damage in kidney genomic DNA of Swiss albino mice: the protective effect of
Ellagic acid. Molecular and Cellular Biochemistry. 2012 Jun; 365(1-2):119-27.
[PMID: 22286819]. Impact Factor: 2.388.
23. Hasan SK, Khan R, Ali N, Khan AQ, Rehman MU, Tahir M, Lateef A, Nafees S,
Mehdi SJ, Rashid S, Shahid A, Sultana S. 18-β Glycyrrhetinic acid alleviates 2-
Acetylaminofluorene induced hepatotoxicity in wistar rats: Role in hyperproliferation,
inflammation and oxidative stress. Human and Experimental Toxicology, 2014
(accepted, in press). Impact Factor: 1.453.
24. Lateef A, Rehman MU, Tahir M, Khan R, Khan AQ, Qamar W and Sultana S.
Farnesol protects against intratracheally instilled cigarette smoke extract-induced
histological alterations and oxidative stress in prostate of Wistar rats. Toxicology
International 2013 Jan;20(1):35-42. [PMID: 23833436]. Impact Factor: Not yet.
25. Rehman MU, Tahir M, Ali F, Qamar W, Khan R, Khan AQ, Lateef A, Hamiza OO,
Sultana S. Chemopreventive effect of Quercus infectoria against chemically induced
renal toxicity and carcinogenesis. International Journal of Drug Development &
Research, 2012, 4 (2): 336-351. Impact Factor: Not Yet.
26. Rehman MU, Tahir M, Khan AQ, Khan R, Lateef A, Hamiza OO, Kazim S, Ali F,
Sultana S. Methanolic extract of Bacopa monnieri protects against KBrO3-induced
renal damage in Wistar rats by restoring antioxidant enzyme activities and
suppressing inflammation and apoptosis. Journal of Pharmacy Research,
2012,5(11),5231-5238. Impact Factor: Not Yet.
27. Hamiza OO, Rehman MU, Tahir M, Khan R, Lateef A, Khan AQ, Qamar W,
Sultana S. Methanolic extract of Terminalia chebula protects against DMH-induced
colon damage in Wistar Rats by restoring antioxidant enzyme activities and
suppressing inflammation. International Journal of Drug Development &
Research, 2014, Vol. 6, Issue 1, ISSN 0975-9344. Impact Factor: Not Yet.
Abstracts:
1. Muneeb U Rehman, Mir Tahir, Farrah Ali, Abdul Quaiyoom Khan, Rehan Khan,
Abdul Lateef, Oday O Hamiza, Wajhul Qamar, Sarwat Sultana.
Chrysin Suppresses Renal Carcinogenesis Via Amelioration of Hyperproliferation,
Oxidative Stress and Inflammation: Plausible Role of NF-κB .
Abstract, Society for Free Radical Biology and Medicine (SFRBM), Free Radical
Biology and Medicine, Volume 53, Supplement 2, 1 November 2012, Page S50.
2. Rehan Khan, Abdul Khan and Sarwat Sultana.
Glycyrrhizic acid modulates 1,2-dimethylhydrazine-induced aberrant crypt foci and
mucin depleted foci via regulating the hyperproliferation, inflammation, angiogenesis
and apoptosis. Poster presentation at European Association for Cancer Research
(EACR)- Irish Association for Cancer Research (IACR) Joint Conference "The
Tumour Microenvironment", September 2012, Dublin, Ireland.
3. Rehan Khan, Abdul Quaiyoom Khan, Wajhul Qamar and Sarwat Sultana.
Farnesol modulates 1,2-Dimethylhydrazine induced oxidative damage in colon of
Wistar Rats.
Poster presentation at XXIX Annual Conference of Society of Toxicology (STOX),
India and International Symposium on “Current Trends in Toxicology and Pre-clinical
Toxicology.
4. R Khan, AQ Khan, W Qamar, A Lateef, MT Maqbool, MU Rehman, F Ali, N Ali, S
Sultana.
Farnesol attenuates 1,2-dimethylhydrazine-induced oxidative stress, inflammation
and apoptotic responses in the colon of Wistar rats.
Poster presentation at 30th Annual Conference of Society of Toxicology (India) and
Symposium on Strategies for Safety Study Requirements for Herbal Formulations
5. Muneeb U Rehman, Mir Tahir, Rehan Khan, Abdul Quaiyoom Khan, Abdul Lateef,
Farrah Ali, Oday O Hamiza, Tyan Jain and Sarwat Sultana.
Chrysin attenuates cisplatin induced hepatotoxicity: abrogation of oxidative stress
and inflammatory response.
Poster presentation at 30th Annual Conference of Society of Toxicology (India) and
Symposium on Strategies for Safety Study Requirements for Herbal Formulations
6. Abdul Quaiyoom Khan, Rehan Khan, Wajhul Qamar and Sarwat Sultana.
Amelioration of ethanol induced liver injury by perillyl alcohol.
Poster presentation at XXIX Annual Conference of Society of Toxicology (STOX),
India and International Symposium on “Current Trends in Toxicology and Pre-clinical
Toxicology.
7. MU Rehman, M Tahir, F Ali, W Qamar, A Lateef, R Khan, AQ Khan, K Anjum, N
Ali, S Sultana.
Quercus infectoria abrogates TCE (Trichloroethylene) induced renal injury in wistar
rats.
Poster presentation at XXIX Annual Conference of Society of Toxicology (STOX),
India and International Symposium on “Current Trends in Toxicology and Pre-clinical
Toxicology.
8. M Tahir, MU Rehman, W Qamar, A Lateef, AQ Quaiyoom, F Ali, K Anjum, R Khan,
S Sultana.
Hepatoprotective effects of chrysin on ethanol induced oxidative stress in Wistar
rats.
Poster presentation at XXIX Annual Conference of Society of Toxicology (STOX),
India and International Symposium on “Current Trends in Toxicology and Pre-clinical
Toxicology.
9. F Ali, MU Rehman, W Qamar, M Tahir, G Kaur, A Lateef, R Khan, AQ Khan, A
Wani, K Anjum, S Nafees, N Ali, S Sultana.
Repeated immobilization stress enhances oxidative burden polyamine synthesis and
PCNA expression induced by co-exposure of trichloroethylene and UVB radiation in
murine skin.
Poster presentation at XXIX Annual Conference of Society of Toxicology (STOX),
India and International Symposium on “Current Trends in Toxicology and Pre-clinical
Toxicology.
10. AQ Khan, R Khan, W Qamar, A Lateef, MT Maqbool, M Rehman, N Ali, S
Rashid, S Sultana.
Perillyl alcohol protects ethanol induced liver toxicity in wistar rats by the attenuation
of oxidative stress and inflammation.
Poster presentation at 30th Annual Conference of Society of Toxicology (India) and
Symposium on Strategies for Safety Study Requirements for Herbal Formulations
11. A Lateef, AQ Khan, W Qamar, M Tahir, M Rehman, R Khan, N Ali, S Sultana.
Effects of nonsteroid anti-androgen flutamide on prostate gland of Wistar rats:
reversal by daidzein.
Poster presentation at 30th Annual Conference of Society of Toxicology (India) and
Symposium on Strategies for Safety Study Requirements for Herbal Formulations
12. W Qamar, A Lateef, AQ Khan, MT Maqbool, R Khan, MU Rehman, N Ali, S
Rashid, S Sultana.
Pulmonary inflammation and damage imposed by intratracheal administration of
benzo(a)pyrene in wistar rats: abrogation by glycyrrhizic acid.
Poster presentation at 30th Annual Conference of Society of Toxicology (India) and
Symposium on Strategies for Safety Study Requirements for Herbal Formulations.
Conferences, Seminars and Workshops:
1. EACR-IACR special conference “The Tumour Microenvironment” Dublin, Ireland in
September 2012.
2. Indo-UK Seminar on “Biomarkers of Ecotoxicity, cancer, Monitoring and
Prevention” held at Jamia Hamdard in 2004.
3. XXIX Annual Conference of Society of Toxicology (STOX), India and International
Symposium on “Current Trends in Toxicology and Pre-clinical Toxicology” held at
Food and Drug Toxicology Research Centre, National Institute of Nutrition, Indian
Council of Medical Research, Hyderabad, in 2009.
4. 30th Annual Conference of Society of Toxicology (India) and Symposium on
Strategies for Safety Study Requirements for Herbal Formulations, held at Jamia
Hamdard, New Delhi in 2010.
5. Manuscript Writing Workshop, held at Jamia Hamdard, New delhi in 2011.
6. Hands-on Training on Flow Cytometry by BD Biosciences, at Jamia Hamdard
(BD-JH FACS Academy), New Delhi in February 2013.
Research Experience:
I have been working as a post-doctoral research fellow at CancerCare
Manitoba, University of Manitoba, Winnipeg, MB, Canada. I was working in the
field of cancer biology. In my post-doctoral research, I have been working on
“Synthetic lethality” for the specific killing of tumor cells without affecting normal
cells via exploiting mutations in chromosomal instability (CIN) genes. CIN is a
hallmark of most solid tumors that cause tumor aneuploidy and tumor
heterogeneity. More than 80% of colorectal cancers exhibit CIN phenotypes that
drive tumorigenesis which often arises from errors in DNA repair. Mutations in CIN
genes therefore differentiate cancer cells from the normal cells. The concept of
“synthetic lethality” was first reported in Drosophila and is very well established in
yeast. Mostly genes are evolutionary conserved and it has been proved that
genes which are having synthetic lethal interaction in yeast, their human orthologs
also have synthetic lethal interaction. By using cross-species candidate gene
approaches from yeast to humans, we have been trying to identify and validate
synthetic lethal interactor of CIN genes. I have been working on project in which I
was exploring the synthetic lethal interaction between superoxide dismutase 1
(SOD1) and CHEK2; and SOD1 and BLM. CHEK2 and BLM play a role in
homologous recombination repair (HRR) pathway and are putative CIN genes
which are responsible for the maintenance of chromosome stability. We have
been trying to knockdown the expression of SOD1 by using RNAi in CHEK2-
deficient and BLM-deficient HCT116 colorectal cancer cells. Further, chemical
inhibitors of SOD1 like LCS-1 and ATTM demonstrated the ability to recapitulate
the synthetic lethal phenotypes as observed with above RNAi experiments. To
carry out this research, I used mammalian cell culture, indirect
immunofluorescence (IIF), western blotting, Real-Time Cell Analyser-Dual Plate
System for label-free cell analysis like cell death, cell proliferation and cell cycle
arrest; and Cytation3 for high-content imaging.
I have completed Ph.D. from Jamia Hamdard (Hamdard University), New
Delhi, India and the title of the thesis was “Colon Cancer in Wistar Rats:
Chemoprevention by Natural Products”. During my Ph.D. dissertation, I have
worked on the chemoprevention of colon cancer in Wistar rats by using natural
compounds like farnesol, glycyrrhizic acid, chrysin, geraniol and rutin etc. and to
further explore the mechanism by which the natural compounds exerts their
chemopreventive properties. I have assessed probable mechanisms of all these
natural compounds against 1,2-dimethyhydrazine-induced colon cancer in
Wistar rat model. I have also assessed the efficacy of some of these natural
compounds against early markers of colon cancer like aberrant crypt foci (ACF)
and mucin depleted foci (MDF). Moreover, I have investigated the protective
effects of some natural compounds against DSS-induced colitis and colitis-
associated cancer (CAC) in Wistar rat model.
Dissertation:
Two months Project training (dissertation) in the Department of Hematology, All India
Institute of Medical Sciences (AIIMS), New Delhi, on the topic “Evaluation of
Hematological Disorders using Molecular techniques”.
Techniques Known:
Cell and Molecular Biology Techniques:
Mammalian Cell Culture: 2D and 3D (microtumors) mammalian cell culture.
I have experience with HCT116, MCF-7, and hTERT cell lines.
Real Time Cellular Analyses by using Real Time Cell Analyser-Dual Plate
System (RTCA-DP) for the study of cell death, cell proliferation and
cell cycle arrest.
High Content Imaging by using Cytation3: It has multimode detection
applications and imaging applications for the study of live and fixed cells.
Liquid Handling Station: Washer and Dispenser (EL406 from Biotek)
Transfection Assay (siRNA)
DNA & RNA Isolation
Real time RT-PCR
Fluorescence in-situ hybridization (FISH)
SDS-PAGE
Western Blotting
Immunohistochemistry
Indirect Immunofluorescence
Enzyme linked Immunosorbent assay (ELISA).
Spectrophotometry.
In-vivo Experience (Rat and Mouse Model):
Expert in developing 1,2-dimethylhydrazine/Azoxymethane-induced colon
cancer in Rat model.
Expert in developing Dextran Sodium Sulphate (DSS)-induced colitis in rat
and mouse model
Expert in developing Colitis-Associated Cancer (CAC) model in Wistar rat.
Expert in developing Xenograft Mouse Model.
Well expertise in different routes of drug administration like intraperitoneal
(i.p.), sub-cutaneous (s.c.), intravenous (i.v.) in tail vein, oral gavage or
intragastric intubation, and intrarectal instillation. Collection of broncho-
alveolar lavage fluid (BALF), peritoneal lavage fluid, harvesting mice embryos
after thoracotomy and collection of different organs from mice and rats.
Personal details:
Sex: Male
Nationality: Indian
Declaration:
I declare that the information furnished above is true and authentic to the best of my
knowledge.
(Rehan Khan)
Referees:
Dr. Sarwat Sultana, Ph.D.
Associate Professor,
Department of Toxicology,
Jamia Hamdard (Hamdard University)
New Delhi, INDIA
Email: *********@**********.***
Dr. Abdur Rub, Ph.D.
Assistant Professor,
Department of Biotechnology,
Jamia Millia Islamia,
New Delhi, INDIA
Email: **********@*****.***
Dr. Kirk McManus, Ph.D.
Assistant Professor,
Department of Biochemistry and Medical Genetics,
University of Manitoba
Winnipeg, MB, CANADA
Email: ****.*******@***.*********.**
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