Medical Years Experience

Soonsang Yoon, PhD

*** ****** ******, ****** *******, Boston University School of Medicine

****@**.*** ( 617-***-****

Green card holder

Summary

1. 15+ years experience in molecular and cell biology

2. Special expertise in metabolic diseases

3.

Educations and Credentials

Ph.D. in Biochemistry, 2000 - University of Oxford, Oxford, UK

Masters of Science (MSc.) in Biochemistry, 1994 - Seoul National

University, Seoul, Korea

Bachelor of Science (BSc.) in Microbiology, 1992 - Seoul National

University, Seoul, Korea

Research Experience

Postdoctoral Research Associate, Boston University School of

Medicine, Boston, MA 2013 - present

Research on Human muscle diseases using primary and immortalized

human muscle cells

Research Associate, Boston Biomedical Research Institute, Watertown,

MA 2012 - 2013

Research on Human muscle diseases using primary and immortalized human

muscle cells

Research Fellow, Harvard Medical School / McLean Hospital, Belmont, MA

2007 - 2008

Reprogramming of mouse fibroblast cells into ES-like cells using 4

transcription factors.

Research Fellow, Harvard Medical School / Children's Hospital, Boston, MA

2004 - 2006

Conduct research in evaluation of genes potentially involved in muscle

fusion to improve the efficacy of cell therapy on patients with muscular

dystrophy.

Research Fellow, Harvard Medical School / Children's Hospital, Boston, MA

2000 - 2004

Pursued research goal of developing a technique for directing liver

cells, embryonic stem cells, haematopoietic and other adult cell

populations into cells exhibiting an islet phenotype in order to cure

diabetes

Graduate Student, Biochemistry, Oxford University, Oxford, UK

1997 - 2000

Conducted functional analysis of viral accessory proteins in equine

Infectious Anaemia Virus (EIAV). Participated in development of a novel

non-primate lentiviral vector based on EIAV for gene transfer

applications.

Skills Used in Research

. Cell culture and cell biology - cell line culture, primary cell

isolation and maintenance, transfection, In situ hybridization,

flow cytometry, growth arrest and cell cycle analysis, ELISA

. Molecular biology - recombinant DNA techniques (cloning, site

directed mutagenesis), RT-PCR / real-time Quantitative PCR, Western

blot analysis, immunoprecipitation, Southern blot analysis,

transfection, In vitro transcription / translation

. Viral vector specific techniques - production and transduction of

retroviral vectors (MLV, HIV and EIAV based vectors), gene transfer

into haematopoietic stem cells and embryonic stem (ES) cells,

systemic injection of adenoviral vector in vivo in mice, gene knock-

down by small interfering RNA (siRNA) using viral vectors

. Biochemistry - enzyme assays, various column chromatography,

protein cross linking and labeling

. Immunohistochemistry - histology (frozen section),

immunocytochemistry (chromatic, direct & indirect fluorescence)

. Microscopy - bright & dark field microscopy, fluorescence microscopy,

confocal microscopy

. Animal work (mouse) - haematopoietic stem cell & skeletal muscle

stem/progenitor cell isolation,

preparing single cell suspension from organs, retro-orbital /

tail vein injection

Publications

1. Yoon, Soonsang; Stadler G, Beermann ML, Schmidt EV, Windelborn JA,

Schneiderat P, Wright WE, Miller JB; 'Immortalized myogenic cells from

congenital muscular dystrophy type1A patient recapitulate aberrant

caspase activation in pathogenesis: a new tool for MDC1A research',

Skeletal muscle, 3(1):28, 2013.

2. Yoon, Soonsang, "Co-expressing Pdx1 and Ngn3 in the liver directs few

liver cells into beta-like cells,'' Biochemical and Biophysical

Research Communications, 362(1): 101-6, 2007.

3. Hong, Sunghoi*; Whang, Dong-Yeon*; Yoon, Soonsang; Ramezani, Ali;

Hawley, Robert; Kim, Kwang-soo; "Functional analysis of various

Promoters in lentiviral vectors at different stages of in vitro

differentiation of mouse embryonic stem cells," Molecular Therapy,

15(9): 1630-9, 2007. *equal contribution

1. Yoon, Soonsang; Molloy, Michael; Gussoni, Emanuela; "C6ORF32 is

upregulated during muscle cell differentiation and induces the

formation of filopodia," Developmental Biology, 301:70-81, 2007.

2. Cerletti, Massimiliano*; Molloy, Michael*; Tomczak, Kinga;; Yoon,

Soonsang; Ramoni, Marco; Kho, Alvin; Beggs, Alan; Gussoni, Emanuela;

Melanoma Cell Adhesion Molecule is a novel marker for human fetal

myogenic cells and affects myoblast fusion.," The Journal of Cell

Science, 119:3117-27, 2006. *equal contribution

3. Ivanova, Natalia; Dimos, John; Schaniel, Christoph; Hackney, Jason;

Moore, Kateri; Ramalho-Santos, Miguel; Yoon, Soonsang; Matsuzaki,

Yumi; Mulligan, Richard; Melton, Douglas; Lemischka, Ihor ; "Response

to Comments on 'Stemness': Transcriptional Profiling of Embryonic and

Adult Stem Cells' and 'A Stem Cell Molecular Signature,'" Science,

Vol. 302, No. 564, pp 393d, 2003.

4. Ramalho-Santos, Miguel; Yoon, Soonsang, Matsuzaki, Yumi; Mulligan,

Richard; Melton Douglas; "'Stemness': Transcriptional Profiling of

Embryonic and Adult Stem Cells." Science, Vol. 298, No. 5593, pp. 597-

600, 2002.

5. Yoon, Soonsang; Kingsman, Susan; Kingsman, Alan; Wilson, Stuart;

Mitrophanous, Kyriacos; "Characterization of the Equine Infectious

Anaemia Virus S2 Protein." Journal of General Virology, Vol. 81, pp.

2189-2194, 2000.

6. Mitrophanous, Kyriacos; Yoon, Soonsang; Rohll, Jonathan; Patil, Deva;

Wilkis, Fraser; Kim, Narry; Kingsman, Susan; Kingsman, Alan; Nicholas,

Mazarakis; "Stable Gene Transfer to the Nervous System Using a Minimal

Non-Primate Lentiviral Vector Gene Therapy 6," Gene Therapy Vol. 6,

No. 11, pp. 1808-18, 1999.

7. Chang J., Yoon S., Lhee S., Park I., Jung D., Park Y. and Yim J.;

"Purification and Properties of Novel Calcium-Binding Proteins from

Streptomyces Coelicolor, Journal of Microbiology, Volume 37, No. 1,

pp. 21-26, 1999.

Awards

. Overseas (UK) Research Students Awards (1998 to 2000)

. British Foreign and Commonwealth Office / Korean Ministry of

Science & Technology Joint Scholarship (1998 to 1999)

. Teaching Assistant Scholarship, Department of Microbiology, Seoul

National University, Seoul, Korea (1992 to 1993)

. University Scholarship, Department of Microbiology, Seoul National

University, Seoul, Korea (1989 to 1991)

Invited Talks

. KRIBB, Daejeon, Korea (10/2008)

. Western Region Islet Study Group (WRISG) conference, Aspen, CO, USA

(9/2006)

. KSEA Stem Cell Meeting, Durham, NC, USA (7/2004)

. New England Biological Society, Harvard Medical School, Boston, MA,

USA (6/2004)

Professional Affiliations

. International Society of Stem Cell Research (ISSCR)

. Korean-American Scientists and Engineers Association (KSEA)

. New England Biological Society

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