Project Quality Control
Resume:
Rishikesh Girish Haridas
Apt No ***, **** Wissahickon Avenue, Philadelphia PA 19144 ● ************@*****.**.** ● 315-***-****
EDUCATION:
Master of Science in Molecular Medicine Drexel University College of Medicine, Philadelphia, PA May 2014
Relevant Coursework: Fundamentals in Molecular Medicine I, Viruses and Viral Infections, Immunology,
Immunopathology and Infectious Diseases, Fundamentals of Molecular Medicine II, Bacteria and Bacterial
Infections, Mycology, Fungal Infections and Antibiotics, Parasitology and Parasitic Diseases, Experimenta l
Therapeutics, Cancer Biology, Biostatistics in Veterinary Science, Bio techniques and Laboratory Research I.
Advanced Diploma in Clinical Research Bombay College of Pharmacy, Mumbai, India April 2012
Relevant Coursework: Drug Discovery & Development Process, Introduction to Clinical Research, Ethical
Theories and Foundations, Ethics Review Committee and Informed Consent Process, Patents US Regulatory
Structure, IND, NDA, ANDA, Post Drug Approval Activities, PMS, FDA Audits and Inspections, Designing of
Protocol, CRF, E-CRF, IB, ICF, SOP, Pharmacy-epidemiology, BA/BE Studies .
Bachelor of Technology in Bioinformatics Dr D. Y Patil University, Mumbai, India June 2011
Research Project
“Molecular Modeling and Computational Analysis of Tubercular Receptors and their Indigenous Drugs”.
●Used Bioinformatics Tools to analyze Wild type and Mutant rpoB and KatG tubercular receptors. Carried out
Molecular Modeling simulation of Tubercular Receptors. Carried out different point mutations in the Target
Receptor structure
●Performed Docking and Binding interaction studies between mutant Tubercular Receptor Structure with their
respective target drug (Rifampicin &Isoniazid) and carried out Pharmacophore Mapping and QSAR studies.
LAB SKILLS
Molecular Biology Skills : DNA/RNA isolation & characterization, Southern blotting, Northern blotting, rDNA
technology, PCR, SDS-PAGE, BCA Assay, ELISA, Western blotting, RT-PCR, q-PCR, Nanodrop Spectrophotometer,Plasma membrane protein extraction, DC Protein Assay, Gel Electrophoresis, Si-RNA Transfection, sh-RNA Transfection,
Immunofluorescence, MTT assay, fluorescence in situ hybridization, Flow Cytometry, Plasmid preparation, Ligand Binding
Assays, Glycosyltransferase activity assay, Protein purification.
Microbiology Skills: Media preparation, Slide preparation, sample preparation, aliquoting,Bacteria culturing, Isolation
and quantification, Human iPS/ES cell culture, Transformation, Splitting of cells, Media optimization, Transfection,
Culture preservation, Mammalian cell culture, 3-dimensional cell culture, Cell splitting, Cell viability, Microplate-based
assays, Gram staining, bacterial identification.
Bioanalytical Techniques: HPLC (Affinity, Size Exclusion, Cation Exchange), LC-MS, Mass Spectrometry.
Bioinformatics Skills : SPDV, Modeller, AutoDOCK4, Autodock,Vina,VMD(Visual Molecular Dynamics),NAMD,DS
Visualiser, Ligand Scout, HyperChem, HEX, Argus Lab, Structure-Activity Relationship (SAR), Quantitative structure–
activity relationship, Pharmacophore Mapping Marvin Sketch, ChemSketch, MDL ISIS.
Biological Databases & Tools: NCBI, EMBL, GeneBank, SwissProt, PDB, Prosite, KEGG, Pfam, Peptide Mass, Peptide
Cutter, PeptideBuilder, Q-site Finder, DrugBank, BLAST, FASTA, EXPASY, ClustalW, PyMol, RasMOL, ProtScale,
ProtParam, Microsoft Office, Microsoft Excel
EXPERIENCE:
Research Intern The Wistar Institute (Dr Meenhard Herlyn`s Lab), PA September 2013- April 2014
Research Project 2: “Validation of candidate genes identified in a targeted shRNA screen that are important
for melanoma progression”.
● Cultured 293T and WM9 melanoma cell lines, carried out Transfection of 293T cells with Rac I and Rab II clones.
● Infected WM9 melanoma cell line with shRNA lentiviral particles, validation of knockdown efficiency by Western Blot.
Carried out MTT assay to assess cell viability after knockdown of the candidate genes
●Carried out MTT assay to assess cell viability of shRNA construct infected WM9 cells, validation of protein
expression by Western Blot.
●Quantified gene expression levels through Real time-PCR (qPCR).
Research Project 1: “Role of Primary Cilium in Melanoma Targeted Therapy”.
●Cultured WM9,1205 Lu,WM 3912 WM 3918 human melanoma cell lines.
●Treated Human melanoma cell lines with PLX-4720(BRAF inhibitor) and with different ERK inhibitors.
●Carried out Western Blot to determine protein expression levels of p-ERK and Total –ERK after treating human
melanoma cell lines with PLX-4720(BRAF inhibitor).
●Carried out MTT assay to assess cell viability.
●Performed Immunofluorescence analysis to stain for primary cilium.
Research Volunteer, Dept of Microbiology and Immunology
Drexel University College of Medicine ( Dr.Sandhya Kortagere’s Lab), PA June 2013 – August 2013
Research Project: “Effect of Dopamine agonists on Macrophage s and HEK cells.”
● Determination of Dopamine D3 receptors on U937 differentiated Macrophages.
● Carried out Plasma Membrane protein extraction, DC Protein Assay and Western Blot analysis for determination of
Dopamine D3 receptors on U937 differentiated Macrophages.
● Functional screening of novel D3R compounds with phosphorylated p44/42 MAPK.
Research Volunteer, Dept of Microbiology and Immunology
Drexel University College of Medicine ( Dr.Sonia Navas’s Lab), PA August 2012 – December 2012
Research project “Role of Toll -like Receptor 3-Induced Type I Interferon in Murine Coronavirus Infection of
Macrophage s”.
●Studied the effects of triggering TLR3 with selected ligands on J774A.1 macrophages susceptibility to MHV infection.
● Carried out various techniques Western Blotting Analysis of Viral Proteins, BCA assay, Splitting of Cells.
●Measured and assessed IFN-α and IFN-β production using ELISA.
Research Intern, Diploma in Recombinant DNA Technology Genecity Laboratories, India June – July 2009
● Basic bacterial culture methods, DNA and RNA handling, isolation and quantification.
● Analysis of DNA samples by gel electrophoresis and purification of DNA
● Primer designing and Polymerase Chain Reaction (PCR), Restriction digestion, ligation, competent cells
preparation and transformation
● Southern and western blotting, Protein purification: salt precipitation and chromatography.
Research Intern, Quality Control Department Mahananda Dairy, India March 2009 – May 2009
●Tested Dairy products based on Moisture, Fat Check, Acidity, Total Solid Cont ent (TS), Solid not fat content(SNF).
●Performed various microbiological techniques like dilutions and plating techniques to find out the microbial content in
the dairy product in Microbiological Laboratory.
●Assessed various chemical tests like Alcohol test, Soda test, Starch test, Urea test, Sugar test, Glucose test on different
varieties of milk in Chemical Laboratory and
●Prepared a demo strategy plan for Production, Processing and Marketing of dairy products.
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