Manager Maintenance

**** *** ***** ******, ******

University of Southern California

Los Angeles, CA 90033

H: 323-***-****, C: 443-***-****

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Young Jin Lee, Ph.D.

Summary Organized scientist specialized in Stem Cell, Embryology,

Developmental Biology and transgenic animal production.

Experience in mouse and human IVF. Adept and successful

in writing grant proposals and manuscripts.

Skills & Abilities ( DNA isolation expert ( Mouse handling & surgery

( Preimplantation embryo

( In vitro transcription isolation & culture

( Microbiological techniques ( In vitro fertilization

( Knowledgeable about ( ICSI (intracytoplasmic

subcloning sperm injection)

( Western blotting ( Pronuclear microinjection

( Expert in data analysis

( Experimental design ( Embryo transplantation

( Nucleic acids isolation ( Other basic gene

( Primer design targeting techniques for

( Plasmid synthesis producing transgenic mice

( Immunofluorescence assay

( Expert in genomic software

Experience Research Associate

University of SOuthern california-los angeles, ca

[Aug 2013 to Current]

( Conducted basic and applied research on identifying the

regulatory process that maintain, expand and

differentiate kidney stem cells to the mature cell types

of the functional kidney.

( Producing transgenic mice using pronuclear

microinjection and blastocyst injection.

postdoctoral research fellow

university of maryland-baltimore, md

[Sep 2008 to Jun 2013]

( Collected and analyzed biological data on evaluating

functional role of cellular prion protein in maintenance

and differentiation of human embryonic stem cells.

lab manager

aSAN MEDICAL CENTER-SEOUL, SOUTH KOREA

[May 2006 to Aug 2008]

( Managed all laboratory members and supplies for all

basic and clinical IVF (In Vitro Fertilization) courses.

Instructor

eulji university-sungnam, SOUTH KOREA

[Jan 2005 to Jan 2007]

( Teaching (Molecular biology, major course,

Undergraguate).

Clinical researcher

asan medical center-seoul, SOUTH KOREA

[Aug 2003 to Mar 2006]

( Conducted molecular and cell biological research on

infertility and stem cells.

research assistant

hanyang university-seoul, SOUTH KOREA

[Mar 2000 to Jul 2003]

( Assisted classes of Experiments of Molecular Biology,

Molecular Genetics and General Biology (major course,

undergraduate).

Education Ph.D., Stem Cell & Developmental Biology (2008)

Hanyang University-Seoul, South Korea

M.S., Stem Cell & Developmental Biology (2002)

Hanyang University-Seoul, South Korea

B.S., Life Science(2000)

Hanyang University-Seoul, South Korea

accomplishments ( Served on IVF laboratory as a clinical researcher for 2

years and manager for 3 years.

( Production of several transgenic mice.

( Published in several journals, including Oncology

Reports, FASEB J, Plos One, The journal of

Neurochemistry, etc.

( Served as the "Invited Reviewer" of Plos One.

( Awarded and completed Postdoctoral Fellowship Grant

from Maryland Stem Cell Research Fund (MSCRF)

honors & awards 2005 Keystone Symposia Scholarship Award

sponsored by National Institute of Genera Medicine

Sciences (NIGMS, NIH, USA)

2007 The Excellent Employee Award (Asan

Medical Center, Seoul, South Korea)

2010 Postdoctoral Fellowship Grant Award,

Maryland Stem Cell Research Fund (MSCRF, MD, USA)

2010 Entrusted as a KOSEN expert to be in

charge of expert consultation (Korea Institute of Science

and Technology Information, Ministry of Science and

Technology, South Korea)

additional Nationality: Korea Republic of (South Korea) with

information Permanent Residence status (Green Card) in US

Publications 22. Lee YJ and Baskakov IV. The cellular form of the

prion protein guides the differentiation of human

embryonic stem cells into neuron-, oligodendrocyte- and

astrocyte-committed lineages. (2014) Prion [in print]

21. Lee A, Kim H, Lee YJ, Lee S, Kim WJ, Han S, Yang S,

Woo H, Na S, Song H, Hong S. Immunolocalization and

estrogen regulation of small proline-rich protein 2a

protein in the mouse uterus (2014) Reprod Fert Develop

26: 682-689

20. Gonzalez-Montalban N, Lee YJ, Makarava N, Savtchenko

R, Baskakov IV. Changes in prion replication environment

cause prion strain mutation. (2013) FASEB J. 27:

3701-3710

19. Won JY, Lee YJ, Lee S, Lim WJ, Han S, Yang S. Woo HM,

Park S, Lee H, Hong S. SPARC is involved in the

maintenance of mitotically inactivated mouse embryonic

fibroblast cells. (2013) In Vitro Cellular &

Developmental Biology 49: 458-464

18. Lee YJ and Baskakov IV. The cellular form of the

prion protein is involved in controlling cell cycle

dynamics, self-renewal and the fate of human embryonic

stem cell differentiation. (2013) J. Neurochem. 124:

310-322 (The cover figure of an issue)

17. Lee YJ, Savtchenko R, Ostapchenko VG, Makarava N,

Baskakov IV. Molecular structure of amyloid fibrils

controls the relationship between fibrillar size and

toxicity (2011). Plos One 6: e20244

16. Lee YJ and Baskakov IV. Treatment with normal prion

protein delays differentiation and helps to maintain high

proliferation activity in human embryonic stem cells.

(2010) J. Neurochem. 114: 362-373 (The cover figure of

an issue)

15. Lee YJ, Nah HY, Hong SH, Lee JW, Jeon I, Pak JH, Huh

JR, Kim SH, Chae HD, Kang BM, Kim CG, Kim CH. Spa-1

regulates the maintenance and differentiation of human

embryonic stem cells. (2008) Int. J. Dev. Biol. 52: 43-53

14. Lee SR, Kim SH, Lee YJ, Hong SH, Chae HD, Kim CH,

Kang BM, Choi YM. Expression of epidermal growth factor,

fibroblast growth factor-2, and platelet-derived growth

factor-A in the eutopic endometrium of women with

endometriosis (2007). J. Obstet. Gynaecol. Res. 33:

242-247

13. Koo YH, Kim CH, Kim JS, Lee YJ, Kim SH, Chae HD,

Kang BM. Expression of osteopontin in eutopic and

ectopc endometrial tissues in endometriosis (2007) Korean

J. Reprod. Med. 34: 149-157

12. Heo HS, Kim JH, Lee YJ, Kim SH, Cho YS, Kim CG.

Microarray profiling of genes differentially

expressed during erythroid differentiation of murine

erythroleukemia cells (2005). Mol. Cells. 20: 57-68

11. Choi HS, Kim SJ, Lee YJ, Cha KJ, Kim CG. Statistical

analysis about ability to mouse embryonic stem cell

differentiation using cDNA microarray (2005). J. Kor.

Data. Infor. Sci. Soci. 16: 951-958

10. Choi JW, Lee HA, Lee SR, Hong SH, Nah HY, Lee YJ,

Kim SH, Chae HD, Kim CH, Kang BM. The expression of

epidermal growth factor and fibroblast growth factor-2 in

the eutopic endometrium of women with endometriosis

(2005). Kor. J. Obstet. Gynecol. 48: 701-708

9. Choi JW, Kim CH, Lee HA, Hong SH, Nah HY, Lee YJ,

Kim SH, Chae HD, Son YS, Kang BM. Efficacy of controlled

ovarian hyperstimulation using GnRH antagonist in women

with polycystic ovary syndrome undergoing IVF-ET (2005).

Kor. J. Obstet. Gynecol. 48: 716-725

8. Choi JW, Kim CH, Lee HA, Hong SH, Nah HY, Lee YJ,

Kim SH, Chae HD, Son YS, Kim BM. The effect of oral

contraceptive pre-treatment in controlled ovarian

hyperstimulation using GnRH antagonist on IVF-ET outcome

(2005). Kor. J. Obstet. Gynecol. 48: 955-961

7. Choi JW, Kim CH, Lee HA, Hong SH, Nah HY, Lee YJ,

Kim SH, Chae HD, YS Son, Kim BM. The efficacy of oral

contraceptive pre-treatment in controlled ovarian

hyper-stimulation using GnRH antagonist in low responders

(2005). Kor. J. Obstet. Gynecol. 48: 987-995

6. Hong SH, Nah HY, Lee YJ, Lee JW, Park JH, Kim SJ,

Lee JB, Yoon HS, Kim CH. Expression of estrogen

receptor-? and -?, glucocorticoid receptor, and

progesterone receptor genes in human embryonic stem

cells and embryoid bodies (2004) Mol. Cells. 18: 320-325

5. Hong SH, Nah HY, Lee JW, Lee YJ, Lee JW, Gye MC, Kim

CH, Kang BM, Kim MK. Estrogen regulates the

expression of the small proline-rich 2 gene family in the

mouse uterus (2004) Mol. Cells. 17: 477-484

4. Hong SH, Nah HY, Lee YJ, Lee JW, Son YS, Chae HD, Kim

SH, Kang BM, Kim CH. Expression of Ids in preimplantation

mouse embryos (2004) Kor. J. Fertil. Steril. 31: 201-207

3. Park JY, Kim CH, Hong SH, Lee YJ, Hong SW, Kwon HE,

Kim SH, Chae HD, Kang BM. Expression of vascular

endothelial growth factor mRNA in eutopic and ectopic

endometrium tissues of patients with endometriosis

(2004). Kor. J. Obstet.Gynecol. 47: 515-522

2. Shin JH, Yi JK, Lee YJ, Kim AE, Park MA, Kim SH, Lee

H, Kim CG. Development of artificial chimerical gene

regulatory elements specific for cancer gene therapy

(2003) Oncol. Rep. 10: 2063-2069

1. Lee YJ, Hong SH, Nah HY, Chae JH, Jung HS, Kim BS,

Kim CG. [Proceeding on the international symposium

on Developmental Engineering] (2001) Expression profiling

of genes involved in the control of pluripotency using

cDNA microarray. pp 18-24

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