Assistant University

Marco Prisco, Ph.D.

Curriculum Vitae

*** ******* *******

Cherry Hill, NJ 08034

Tel: 215-***-**** email: ***********@*****.***

USA citizenship.

Skills: Molecular Biology/Cell Biology/Cancer Biology/Cell Signaling/Cell

Cultures/Epigenetics/Cell migration/invasion/Cell-based assay

development/RNAi, ChIP, qRT-PCR, FACS/ Protein expression/DNA Methylation

. Tissue culture of human and mouse cell lines of different origins,

including mouse embryonic stem cells.

. Identification of Pathways involved in self renewal and differentiation

of stem cells, protection of apoptosis and survival of cancer cells.

. Cell surface tyrosine kinase receptor signaling studies.

. Generation of stable cell lines overexpressing wild type or mutated

proteins.

. In Vitro assays; Cell growth and differentiation assays; cell

transformation assays, including colony formation in soft-agar and

methylcellulose; alkaline phosphatase assays; invasion assays;

pharmacologic assays.

. Delivery of genes, oligonucleotides, siRNA and microRNA in cell lines

using various transfection/transduction methods, including

electroporation, nucleofection, retoviral infection and lentiviral

infection.

. Generation of stable cell lines by gene targeting using antisense, siRNA

and microRNAs.

. Construction and cloning of various vectors, including retrovirus and

lentiviruses.

. Analysis of Nucleic acids by Northen, Southern blot, PCR, RT-PCR.

. Protein purification, characterization and analysis by Western blot,

immunoprecipitation, co-immunoprecipitation, assays for proteins

phosphorylation, ChIP, ELISA.

. Luciferase assays, B-gal quantification, preparation of sample for the

analysis of differentiation markers by FACS, Confocal microscopy, and

immunocytochemistry.

. HPLC.

. Flank injection of tumor cell in mice (Xenografting).

. Train and supervise Technicians, PhD students and Post-doctoral Fellow in

the design and execution of several research projects.

. Preparing reports, posters, publications and presentation of scientific

projects outcome.

. Strong computer, scientific and organizational skills.

. Excellent oral and written communication and attention to detail.

. Ability to work independently and as part of a team, self-motivated,

adaptability and positive attitude.

. Ability to perform multiple tasks simultaneously, ability to learn

quickly new techniques.

Education:

Ph.D. (Doctorate Degree) Molecular, Diagnostic and Quantitative Pathology,

University of Siena, Siena, Italy. Experimental thesis entitled "Insulin

Receptor Substrate 1 in cell differentiation and transformation"

2006

Examination for the Italian National Board of Biologists, Bari, Italy

1993

Doctor in Biological Sciences, University of Bari, Bari, Italy,

1993

Experimental thesis entitled "DNA hypomethylation during liver cell

proliferation induced by lead nitrate".

Intern in Comparative Biochemistry, Department of Biochemistry and

Molecular Biology, University of Bari, Bari, Italy. DNA methylation and

carcinogenesis. 1988 - 1992

Academic Positions or Appointments:

Temple University, Philadelphia, PA

Fels Institute

Scientist/Researcher 2012-Present

Temple University, Philadelphia, PA 2011-

Present College of Science and Technology, Department of Biology

Senior Scientist/Adjunct Assistant Professor/Researcher

Studies aimed at the understanding the role of Insulin Receptor Substrate 1

and 2 in breast cancer primary tumor formation and generation of

metastasis.

Thomas Jefferson University, Philadelphia, PA 2009

- 2012

Department of Stem Cell Biology and Regenerative Medicine

Senior Scientist/Research Assistant Professor

Studies aimed at the understanding on how high-energy metabolites (lactate

and ketones) influence tumor growth behavior in breast cancer cells using

exon-array gene analysis.

Thomas Jefferson University, Philadelphia, PA 2006 -

2009

Department of Cancer Biology

Senior Scientist/Research Assistant Professor

Expression regulation of Gfi-1 transcriptional repressor by C/EBP? and its

involvement in cell proliferation and colony formation in p210BCR/ABL-

expressing cells.

Studies on the transcriptional repression of c-Myb and GATA-2 and their

involvement in the biological effects of C/EBP? in p210 BCR/ABL-expressing

cells.

Thomas Jefferson University, Philadelphia, PA 2003 -

2006

Department of Pathology Anatomy and Cell Biology,

Senior Scientist/Research Assistant Professor

Regulation of self-renewal and differentiation processes by Insulin

receptor substrate (IRS)-1 in murine embryonic stem (mES) cells.

Thomas Jefferson University, Philadelphia, PA 2001 -

2003

Kimmel Cancer Center,

Department of Microbiology and Immunology

Scientist/Research Instructor

Inhibition of colon cancer cells growth by Insulin Receptor Substrate-1

targeting employing MicroRNA 145.

Study of microRNA expression profiles in proliferating and differentiating

32D murine myeloid cells.

Development of a modified TAP tag technique for the purification of protein

complexes in mammalian cells.

Regulation of upstream binding factor 1 activity by IGF-I receptor

signaling in cell transformation and differentiation processes.

The role of Pescadillo and Upstream Binding Factor in the proliferation and

differentiation of murine myeloid cells.

Thomas Jefferson University, Philadelphia, PA 1999 -

2001

Kimmel Cancer Center,

Department of Microbiology and Immunology

Research Associate in the laboratory of Dr. Renato Baserga

IGF-I Receptor signaling and nuclear translocation of Insulin Receptor

Substrates-1 and 2.

Signaling differences from the A and B isoforms of the Insulin Receptor in

32D cells in the presence or absence of IRS-1

Nuclear translocation of IRS-1 by the SV40 T antigen and the activated IGF-

IR.

Thomas Jefferson University, Philadelphia, PA 1994 -

1999

Kimmel Cancer Center,

Department of Microbiology and Immunology

Postdoctoral Fellow in the laboratory of Dr. Renato Baserga

Studies of the multiple pathways of the IGF-I receptor in protection from

apoptosis.

Regulation of Id gene expression by the type 1 Insulin-Like Growth Factor.

Anti-apoptotic signaling of the IGF-I receptor through mitochondrial

translocation of c-Raf and Nedd4.

Oncology Institute of Bari, Bari, Italy

1993 - 1994

Department of Molecular and Cell Biology

Postdoctoral Fellow in the laboratory of Dr. Mario De Lena

HerB2 oncogene expression in breast cancer.

Editorial activities:

Editorial Board Member of "The American Journal of Pathology"

2008 - Present

Scientific Editorial Member of "Impact Journals of Aging"

2008 - Present

Editorial Board Member of "h Biosciences" 2008 -

Present

Manuscript Reviewer, Cancer Research

Manuscript Reviewer, The American Journal of Pathology

Manuscript Reviewer, Endocrinology

Manuscript Reviewer, Cell Cycle

Manuscript Reviewer, Oncogene

Manuscript Reviewer, Journal of Cell Physiology

Grants Reviewer, National Institute of Health

2009

Grants Reviewer, Philip Morris External Research Program 2006 -

2008

Consultant: Ste-Justine Hospital Research Center,

2004

Universit de Montr al, Montreal H3T 1C5

Book Reviewer and Expert Opinion Writer: Stem Cell Handbook, Humana Press

Inc. 2004

Honors and Awards:

Full Member of the Graduate Faculty 2004 -

Present

Nominated as one of the leading healthcare professionals

from around the world: Who's Who in Medicine and Healthcare (8th Edition)

2011-2012

Professional Organization Memberships:

American Association for Cancer Research 1998 -

2004 American Association for the Advancement of Science

1998 - 2004

Master of Science and Ph.D. Thesis Committees:

2009-2012 Pharmacology Master Program, Thesis Committee Member.

Student: Tiziana deAngelis

2003-2007 Genetics Ph.D. Program, Thesis Committee Member.

Student: Bin Shi

2001-2003 Pharmacology Master Program, Thesis Committee Member.

Student: Xiao-Tu

Invited speaker in scientific meetings:

Nuclear translocation of Insulin receptor substrate 1 in cell

transformation. Department of Neurology, Temple University, Philadelphia,

Pennsylvania 2007.

Role of IGF-IR in cell differentiation and transformation processes.

University of Bari, Bari, Italy 2006.

Role of Insulin receptor substrate 1 in cell transformation. University of

Siena, Siena, Italy 2006.

Role of IRS-1 in mouse embryonic stem cells self-renewal. Taormina, Italy

2005.

DNA hypomethylation during liver cell proliferation induced by a single

dose of lead nitrate. Turi, Italy 1991.

Peer-Reviewed publications:

1) Martinez-Outschoorn UE, Prisco M, Ertel A, Tsirigos A, Lin Z,

Pavlides S, Wang C, Flomenberg N, Knudsen ES, Howell A, Pestell RG,

Sotgia F, Lisanti MP. Ketones and lactate increase cancer cell

"stemness" driving recurrence, metastasis, and poor clinical

outcome in breast cancer: Achieving personalized medicine via

Metabolo-Genomics. Cell Cycle. 2011 Apr 15; 10(8)

2) Lidonnici MR, Audia A, Soliera AR, Prisco M, Ferrari-Amorotti G,

Waldron T, Donato N, Zhang Y, Martinez RV, Holyoake TL, Calabretta

B. Expression of the transcriptional repressor Gfi-1 is regulated

by C/EBP{alpha} and is involved in its proliferation and colony

formation-inhibitory effects in p210BCR/ABL-expressing cells.

Cancer Res. 2010 Oct 15; 70(20):7949-59.

3) Soliera AR, Lidonnici MR, Ferrari-Amorotti G, Prisco M, Zhang Y,

Martinez RV, Donato NJ, Calabretta B. Transcriptional repression of

c-Myb and GATA-2 is involved in the biological effects of

C/EBP{alpha} in p210 BCR/ABL-expressing cells. Blood. 2008 Sep

1;112(5):1942-50

4) Rubin R, Arzumanyan A, Soliera AR, Ross B, Peruzzi F, Prisco M.

Insulin receptor substrate (IRS)-1 regulates murine embryonic stem

(mES) cells self-renewal. J Cell Physiology. 213: 445-53, 2007.

5) Shi B, Sepp-Lorenzino L, Linsley P, Prisco M, deAngelis T, and

Baserga R. Micro RNA 145 Targets the Insulin Receptor Substrate-1

and Inhibits the Growth of Colon Cancer Cells. J Biol Chem.

282:32582-90, 2007.

6) DeAngelis T, Chen J, Wu A, Prisco M, and Baserga R. Transformation

by the Simian Virus 40 T Antigen is regulated by IGF-I Receptor and

IRS-1 signaling. Oncogene 25:32-42, 2006.

7) Shi B, Prisco M, Calin G, Liu CG, Russo G, Giordano A, Baserga R.

Expression profiles of micro RNA in proliferating and

differentiating 32D murine myeloid cells. J Cell Physiology 207:

706-10, 2006.

8) Drakas R, Prisco M, Baserga R. A modified TAP tag technique for the

purification of protein complexes in mammalian cells. Proteomics

5:132-137, 2005.

9) Wu A, Tu X, Prisco M, and Baserga R. Regulation of upstream binding

factor 1 activity by IGF-I receptor signaling. J Biol Chem.

280:2863-72, 2005

10) Liu M, Prisco M, Drakas R, Searles D, and Baserga R. 24p3 in

differentiation of myeloid cells. J. Cell Physiology 205:302-309,

2005.

11) Bergonzini V, Delbue S, Wang JY, Reiss K, Prisco M, Amini S,

Khalili K, and Peruzzi F. HIV-Tat promotes cellular proliferation

and inhibits NGF-induced differentiation through mechanisms

involving Id1 regulation. Oncogene, 23(46): 7701-11, 2004.

12) Prisco M, Maiorana A, Guerzoni C, Calin G, Calabretta B, Voit R,

Grummt I, and Baserga R. The role of Pescadillo and Upstream

Binding Factor in the proliferation and differentiation of murine

myeloid cells. Mol. Cell. Biol. 24: 5421-5433, 2004.

13) Sun HZ, Tu X, Wu A, Prisco M, Casaburi I, and Baserga R. IGF-I

Receptor signaling and nuclear translocation of Insulin Receptor

Substrates-1 and 2. Mol Endocrinol. 17(3):472-86, 2003.

14) Sciacca L, Prisco M, Wu A, Belfiore A, Vigneri R, and Baserga R.

Signaling differences from the A and B isoforms of the Insulin

Receptor in 32D cells in the presence or absence of IRS-1.

Endocrinology. 144(6):2650-8, 2003

15) Tu X, Baffa R, Luke S, Prisco M, and Baserga R. Intracellular

redistribution of nuclear and nucleolar proteins during

differentiation of 32D murine hemopoietic cells. Exp Cell Research.

288(1):119-30. 2003.

16) Belletti, B., Drakas, B., Morrione, A., Tu, X., Prisco, M., Yuan,

T., Caseburi, I., and Baserga, R. Regulation of Id1 protein

expression in mouse embryo fibroblast by the type 1 insulin like

growth factor receptor. Exp. Cell Research. 277: 107-118, 2002.

17) Prisco M, Santini F, Baffa R, Liu M, Drakas R, Wu A, and Baserga R.

Nuclear translocation of IRS-1 by the SV40 T antigen and the

activated IGF-IR. J. Biol. Chem. 277(35): 32078-85, 2002.

18) Tu X, Batta P, Innocent N, Prisco M, and Baserga R. Nuclear

translocation of Insulin Receptor Substrate-1 by oncogenes and IGF-

1. J. Biol. Chem. 277: 44357-65. 2002.

19) Belletti B, Prisco M, Morrione A, Valentinis B, Navarro M, and

Baserga R. Regulation of Id2 Gene Expression by the IGF-I Receptor

Requires Signaling by Phosphatidylinositol-3 kinase. J. Biol.

Chem.276: 138**-*****. 2001.

20) Peruzzi F, Prisco M, Morrione A, Valentinis B, and Baserga R. Anti-

apoptotic signaling of the IGF-I Receptor through Mitochondrial

Translocation of c-Raf and Nedd4. J. Biol. Chem. 276: 259**-*****,

2001.

21) Prisco M, Peruzzi F, Belletti B, and Baserga R. Regulation of Id

Gene Expression by the Type 1 Insulin-Like Growth Factor: Roles of

Stat3 and the Tyrosine 950 Residue of the Receptor. Mol. Cell.

Biol. 21: 5447-5458, 2001.

22) Dews M, Prisco M, Peruzzi F, Romano G, Morrione A, and Baserga R.

Domains of the IGF-I receptor required for the activation of

extracellular signal-regulated kinases. Endocrinology 141:1289-

1300, 2000.

23) Gatzka M, Prisco M, and Baserga R. Stabilization of the ras

oncoprotein by the insulin-like growth factor 1 receptor during

anchorage-independent growth. Cancer Res. 60: 4222-4230, 2000.

24) Prisco M, Romano G, Peruzzi F, Valentinis B, and Baserga R. Insulin

and IGF-I Receptors signaling in protection from apoptosis.

Hormones and Metabolic Res. 31:80-89, 1999.

25) Valentinis B, Morrione A, Peruzzi F, Prisco M, Reiss K, and Baserga

R. Anti-apoptotic signaling of the IGF-I receptor in fibroblasts

following loss of matrix adhesion. Oncogene 18:1827-1836, 1999.

26) Valentinis B, Romano G, Peruzzi F, Morrione A, Prisco M, Soddu S,

Cristofanelli B, Sacchi A, and Baserga R. Growth and

differentiation signals by the insulin-like growth factor 1

receptor in hemopoietic cells are mediated through different

pathways. J. Biol. Chem. 274:124**-*****, 1999.

27) Romano G, Prisco M, Zanocco-Marani T, Peruzzi F, Valentinis B, and

Baserga R. Dissociation between resistance to apoptosis and the

transformed phenotype in IGF-I Receptor signaling. J. Cell Biochem.

72:294-310, 1999.

28) Peruzzi F, Prisco M, Dews M, Salomoni P, Grassilli E, Romano G,

Calabretta B, and Baserga R. Multiple pathways of the IGF-I

receptor in protection from apoptosis. Mol. Cell. Biol. 19:7203-

7215, 1999.

29) Xu S-Q, Tang D, Chamberlain S, Pronk G, Masiarz FR, Kaur S, Prisco

M, Zanocco-Marani T, and Baserga R. The granulin/epithelin

precursor abrogates the requirement for the Insulin-like growth

factor 1 receptor for growth in vitro. J. Biol. Chem. 237

(32):200**-*****, 1998.

30) Nolan MK, Jankowska L, Prisco M, Xu S, Guvakova MA, and Surmacz E.

Differential roles of IRS-1 and SHC signaling pathways in brest

cancer cells. Int. J. Cancer. 72:828-834, 1997.

31) D'Ambrosio C, Valentinis B, Prisco M, Reiss K, Rubini M, and

Baserga R. Protective effect of the insulin-like growth factor I

receptor on apoptosis induced by okadaic acid. Cancer Res. 57:3264-

3271, 1997.

32) Prisco M, Hongo A, Rizzo M G, Sacchi A, and Baserga R. The IGF-I

receptor as a physiological relevant terget of p53 in apoptosis

caused by Interleukin-3 withdrawal. Mol. Cell. Biol. 17: 1084-1092,

1997.

33) Kanduc D. and Prisco M. Hepatic DNA methylation in young, middle

aged and senescent rats: effect of mitogen-induced cell

proliferation. Biochem. Med. Metab. Biol. 48:286-291, 1992.

34) Rossiello M R, Aresta A M C, Prisco M, and Kanduc D. DNA

hypomethylation during liver cell proliferation induced by a single

dose of lead nitrate. Boll. Soc. It. Biol. Sper. 12:993-997, 1991

Books and Book chapters:

1) Baserga R, Prisco M, and Yuan T. IGF-1 Receptor Signaling in Health

and Disease. In: Insulin-like Growth Factors, pp 104-120. (D.

LeRoith, W. Zumkeller and R.C. Baxter, Editors.) Plenum Publishers,

2003.

2) Baserga R, Prisco M, and Hongo A. IGFs and cell growth. In:

Contemporary Endocrinology: The IGF System pp 329-353 (R. Rosenfeld

and C. Roberts Jr. eds.), Humana Press, Inc., 1999.

3) Baserga R, Prisco M, and Resnicoff M. The multiple roles of the IGF-I

receptor in cell growth. Molecular mechanisms to regulate the

activities of Insulin-like Growth Factors. K. Takano, N. Hizuka and S-

I. Takahashi (Editors). Elsevier Science B. V. S. 1998.

4) Kanduc D, Prisco M, Bracalello A. Codon bias, tRNA availability and

gene expression regulation. Carcinogenesis as process. D. Kanduc and

P. Pani (Editors), 1992.

Reviews:

1) Baserga R, Morrione A, and Prisco M. Growth factors, cell

proliferation, and apoptosis. Heart Faliure Reviews 3:5-14, 1998.

2) Baserga R, Hongo A, Rubini M, Prisco M, and Valentinis B. The IGF-I

receptor in cell growth, transformation and apoptosis. Biochim.

Biophys. Acta 1332:105-126, 1997.

Summary:

. Tissue culture of human and mouse cell lines of different origins,

including mouse embryonic stem cells and neuronal stem cells.

. Cell surface tyrosine kinase receptor signaling studies and Generation of

stable cell lines overexpressing wild type or mutated proteins.

. In Vitro assays; Cell growth and differentiation assays; cell

transformation assays, including colony formation in soft-agar and

methylcellulose; alkaline phosphatase assays; invasion assays;

pharmacologic assays.

. Delivery of genes, oligonucleotides, siRNA and microRNA in cell lines

using various transfection/transduction methods, including

electroporation, nucleofection, retoviral infection and lentiviral

infection.

. Generation of stable cell lines by gene targeting using antisense, siRNA

and microRNAs.

. Construction and cloning of various vectors, including retrovirus and

lentiviruses.

. DNA and RNA extraction, purification, sequencing.

. Protein purification, characterization and analysis by Western blot,

immunoprecipitation, co-immunoprecipitation, assays for proteins

phosphorylation, ChIP, ELISA, HPLC, immunohistochemistry, fluorescent in

situ hybridization.

. Luciferase assays, B-gal quantification, preparation of sample for the

analysis of differentiation markers by FACS, Confocal microscopy, and

immunocytochemistry.

. Preparation of reports, posters, publications and presentation of

scientific projects outcome.

. Strong computer, scientific and organizational skills.

. Train and supervise Technicians, Master and PhD students and Postdoctoral

Fellows in the design and execution of several research projects.

References:

Renato L. Baserga M.D.

Distinguished Professor

Department of Cancer Biology

Thomas Jefferson University 624 BLSB

233 south 10th Street, Philadelphia PA, 19107

Phone: 215-***-****

*********@******************.***

Bruno Calabretta M.D.

Professor

Department of Cancer Biology

Thomas Jefferson University 630 BLSB

233 south 10th Street, Philadelphia PA, 19107

Phone: 215-***-****

Mail: ************@****.***.***.***

Antonio Giordano M.D., Ph.D.

Professor

Director, Sbarro Institute for Cancer Research and Molecular Medicine

And Center for Biotechnology

College of Science and Technology, Temple University

BioLife Science Bldg. Suite 333

1900 N. 12th Street

Philadelphia, PA 19122

Phone: 215-***-****

Fax: 215-***-****

Mail: *********@****.***

John Pastorino, Ph.D.

Associate Professor of Molecular Biology

School of Osteopathic Medicine

University of Medicine & Dentistry of New Jersey

Science Center, Room 316

Two Medical Center Drive

Stratford, NJ 08084

Tel: 856-***-****

Fax: 856-***-****

Mail: ********@*****.***

Robert A. Drakas, Ph.D.

Senior Director, Business Development and Project Management

ChemPartner Co. USA

US mobile: 215-***-****

Mail: ******@***********.***

Raffaele Baffa M.D., Ph.D.

Senior Director, Translational & Experimental Medicine

Sanofi-Aventis

300 3rd street

Cambridge, MA 02142

Tel: +1-617-***-****

Mail: ********.*****@******.***

Andera Morrione, Ph.D.

Director of Urology Research Labs

Associate Professor, Department of Urology

Thomas Jefferson University

620 BLSB 233 South 10th Street,

Philadelphia, PA 19107

Tel: 215-***-****

Mail: ******.********@*********.***

Michele Rubini, Ph.D.

Professor

Sez. Genetica Medica

Dip. Medicina Sperimentale e Diagnostica

Universit di Ferrara

Via Fossato di Mortara 74

I-44121 Ferrara, Italy

phone: +39.0532.974473

fax: +39.0532.236157

http://docente.unife.it/michele.rubini

Mail: ***@*****.**

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