Tammy Burroughs Hooks
**** ***** ***** **** *******, NC 27610
Phone: 919-***-**** Email: ***********@*****.***
Cell: 919-***-****
ProfesSional Profile: Biologist with over 14 years of academia and
industry experience where my technical, leadership, and customer service
skills have assisted organizations in meeting strategic objectives by
streamlining processes and increasing efficiency
summary of qualifications:
Experience in sequencing, Data management, analysis,
genotyping, aseptic techniques, mining, and self-starter
testing samples, ordering inventory Mentoring/Developing Others
Proficient in molecular genetic Troubleshooting, equipment
techniques maintenance
Process development and protocols Presentation/Training
Understanding of cGMP, CFR21, lean Team player and independent
sigma Strong customer relations and Lead and manage sub-teams
focus Strong negotiation skills
Excellent attention to detail,
written, communications, and
organization skills
Plan and execute special programs
and events
KEY ACCOMPLISHMENTS:
. Discovered 2 novel mutations caused by nickel mutagenesis
. Directed 4 major development/implementation efforts (amplifluor,
primer redesign, mouse DNA purification, 98-plex MAB panels and
protocols)
. Piloted, validated, and collaborated with the UK to streamline global
data management standards for Marker Assisted Breeding (MAB) program
using a Laboratory Information Management System (LIMS) database and
other web-based GSK employees
. Hired, trained and managed 75+ personnel and directed activities of
contractors
RESEARCH/ TECHNICAL SKILLS:
. Equipment: Autoclave, ABI 7000, 7700, and 7900 Taqman, ABI 3700
and 3730 DNA analyzer, Biomek FX automation, Roche Light cycler,
Luminex HTS, Nanodrop, and QuantIt kit
. Analytical agarose gel analysis, spectrometry, microscopy, data
imaging, and mutagenesis assays
. Plant and animal DNA, RNA, PCR extraction, isolation,
purification, cell/tissue culture, and chromatography
. Programs and analysis software: DNA Sequencher, Vector NTI,
Primer Express, Data Collection, BioLims and Genoportal
databases, Nautilus programs, and Spotfire
. Windows NT, MS Word, Excel, Powerpoint, Outlook, and Lotus Notes
EDUCATION:
Fayetteville State University, Fayetteville, NC
. Master of Science in Biology, 2001
Thesis: The specificity of Ni2+-induced mutagenicity during in
vitro DNA synthesis
. Bachelor of Science in Biology (Magna Cum Laude), 1997
EXPERIENCE:
Quality Operations Laboratory Biologist I
11/2013 - Present
Merck (Agile), Durham, NC
. Perform tissue culture based potency assays on live viral
vaccine products using manual and automated cell-based
biological techniques (classic plaque and TCID50 assays)
. Responsible for quantitative cell based infectivity assay,
visual recognition and documentation of cytopathic effect,
multiple mammalian cell line maintenance, prepare solutions and
cell culture media, inventory and re-stocking, logbook keeping,
LIMS data entry and query, routine equipment cleaning and
maintenance
. Work independently and within a team in cGMP and GLP
environments using root cause analysis methodology, excel,
macro, visual basic, lean laboratory knowledge and Six Sigma
training
Customer Care Specialist/Research Telephone Interviewer
09/2013 - 12/2013
RTI International (Greene Resources), Raleigh, NC
. Collect research data and informed consent from survey
participants via phone interviews
. Perform and accurately enter data responses and coding into
computer programs for the CDC
. Communicate to participants the importance of the survey and why
their participation is valuable
Financial Analyst 11/2009
- 09/2013
Primerica, Inc., Greensboro, NC
. Distributed and market financial strategies using NC Life and Health
license
. Communicated with corporate, senior management, and clients to timely
process confidential financial data and maintain compliance procedures
. Recruit, train, and manage over 50 representatives and host
financial seminars/presentations
Molecular Biologist in Plant Analysis
07/2009 - 11/2009
Syngenta Biotechnology, Inc., Research Triangle Park, NC
. Developed and validated molecular assays for genetically modified
crops
. Performed high throughput nucleic acid extraction taqman amplification
using robotics
. Provided technical support and wrote validation assays
Scientist in Molecular Discovery Research
10/2002 - 02/2009
Manpower at GlaxoSmithKline in Genetic Laboratory Sciences
05/2002 - 10/2002
GlaxoSmithKline Inc., Research Triangle Park, NC
. Accessioned/processed preclinical samples, performed QA/QC, validated
and analyzed data for 133+ mouse models for support of world-wide
customers and 5 US sites
. Streamlined processes and reduced cost and time by 50% for breeding
programs as US Project coordinator and ran the DNA Core Sequencing
facility
. Increased efficiency by 50%
writing/standardizing/troubleshooting protocols and developed
operational standards for inventory, forecasting, ordering,
project summaries, and expense reports
. Provided strategic direction as project team leader to 700+
employees and trained 25 peers
Research Assistant in Molecular Genetics
09/1995 - 05/2001
Fayetteville State University, Fayetteville, NC
. Designed experiments to investigate the specificity of nickel-
induced mutagenicity
. Performed DNA hybridizations, transfections, phenotypic plaque
platings on media, ssDNA purification, quantitations, agarose
gel analysis, and maintained culture stocks/bacterial cell lines
. Processed samples for DNA sequencing, analyzed data using DNA
analysis software, constructed mutation spectra for all mutants
and control data, calculated error rates, and mutation
frequencies
References Available Upon Request