RA/Scientist
Resume:
Gursharan Bakshi
***********@*****.***
Yorba Linda, CA-92886.
Objective
To secure a position in the field of research and product development that will enable me to use my diverse
educational background, organizational skills, and ability to work well with people.
Academic Education
California State University Long Beach, CA-90840
MS Biochemistry, May 2012
Guru Nanak Dev University, Amritsar, Punjab – India.
MS Chemistry, April 2000
BS Chemistry and Biology, April 1998
Skill set
Site Directed Mutagenesis Preparation of Buffers/Reagents
PCR Primer Design Titrations
PCR Inorganic Qualitative Analysis
Plasmid DNA Prep Organic Qualitative Analysis (Functional groups)
DNA extraction/purification Synthesis of Organic/Complex compounds
Recombinant Protein Expression & Isolation Detection of Elements
DC Assay pH meter
BSA Assay Titration
ELISA TLC Analysis
Fluorescence Spectroscopy Autoclave
Fluorophore labeling Micro Fluidizer
Tagging Fluorophores to specific Microsoft Excel
amino acid residues in a Protein MS Word
Bacterial Transformation Powerpoint
Affinity Column Chromatography Proficient in MS-Office Suite
Gel Electrophoreses Thompson-Reuters EndNote X2/X4
Antibody Purification Molecular Visualization software for proteins –
CD Spectroscopy Chimera, Rasmol, Jmol
UV-Vis Spectrophotometery Sigma Plot
Nanodrop CLS Sequence Viewer
Centrifugation
Work Experience
Manufacturing Chemist (Sept. 2012 - Oct. 2012)
Alere, Inc. (Immunalysis)
829 Towne Center Dr, Pomona, CA.
ELISA: For forensic matrices such as blood/oral fluids/Urine. Tested drugs of abuse such as Cocaine, Morphine,
Ketamine, Tramadol, Bupernorphine, Cotinine, Carosiprodol, Oxycodone, Mepridine, etc. with ELISA (2-3 Hr process)
using a protocol involving a few microliter of sample drug, HRP conjugate, TMB substrate and Stop solution. Data read
and analysed using Tecan Plate reader
Antibody purification: Using affinity column chromatography with fractionating column and buffers (Wash
buffers/Binding buffers/Elution buffers). Labeling with biotin. Purification of antibodies following SOPs
(unlabeled/labeled with biotin) such as Tramadol, Carisoprodol, Bupernorphine, Cotinine, etc. (2-3 days process)
Preparing ELISA reagents: Preparing and testing HRP Conjugates for QC.
Data recording In MS Excel. Discussion and analysis under Supervisor's guidance.
Completing sample request form following FDA regulations and QA guidelines.
Graduate Researcher (May 2011- Mar 2012)
Thesis Project and Research Article
Research Associate (CSULB) (Dec 2009 – Apr 2011)
Developing tools and techniques for the project
Experimental design.
Conducting and Optimize experimental procedure
Data recording and analysis of experiments with Principal Investigator
Read scientific literature pertaining to project and apply relevant findings to research project.
Maintain laboratory notebook and catalog for up-to-date detailed log of experiments and ongoing research efforts
and maintaining Inventory
Preparing Buffers and Solutions
Write and develop project protocols and methodology for developed research
Conduct complex, high-level scientific research activities
Participation in Seminars, Conference and Competitions
Project Title “Developing Pyrene Fluorescence as a tool to study the conformation of Apolipoprotein E (ApoE).”
The research encompasses the structural and conformational analysis of apoE, which is the major cholesterol
transporter in the brain and plasma. My research spans a range of fields such as rational in silico design of constructs
(Primer Design, PCR, Site Directed Mutagenesis) using the available coordinates from the Protein Data Bank, generating
the mutants using standard molecular biology tools, expressing and purifying the recombinant protein, protein chemistry
to systematically label the variants with the fluorescent probe, and finally perform steady state fluorescence spectroscopic
analysis of the labeled protein. The data outlined in my thesis research is a new piece of structural information, which was
made possible because of the novel fluorescence tool developed in my project. It is believed that this is a significant
contribution to the lipoprotein field since it helps in understanding the structural basis of apoE function in maintaining the
cholesterol homeostasis in the plasma. In addition, this tool can be employed by other protein chemists to study proteins
that are difficult to crystallize.
Other Responsibilities as RA/Staff Scientist
Participation in conferences, seminars, poster/Powerpoint presentations
Maintain lab records/protocols/inventory
Order chemicals/experiment related items/equipment for lab
Train students for research
Perform literature search for research articles
Write drafts for review/ research article
Teaching Assistant / Graduate Assistant for the Dept. of Chemistry & Biochemistry / Research Lab, CSULB
(2008 – 2011)
Teaching labs/activity
Helping students conducting lab experiments
Grading labs/exams/quizzes
Helping prepare results
Lecturer in Chemistry in High School, India (2002 – 2008)
Teaching (Freshmen and Sophomore)
Managing and conducting labs
Preparing and Grading Exams
Co-curricular activities
Awards and Honors
Michael Monahan Memorial Summer Research Fellowship, CSULB – 2011
Second place holder in 23rd annual CSULB Student Research Competition 2011 and Finalist for 25th annual
CSU Student Research competition at CSU Fresno
Eden Award Finalist in 23rd Annual CSU Biotechnology Symposium, Jan 2011, Orange County, CA
Best Teacher for commendable performance and best results in High school classes, India, 2008
Third position in University rankings (throughout the state) in MS Chemistry, India, 2000
Conferences /Meetings Attended
Arteriosclerosis Thrombosis and Vascular Biology (ATVB), Annual Conference, April 27-30, 2011, Chicago, IL.
(Bains, G., Patel, A., Vera, R., Zheng, Y., Bielicki, J. K. and Narayanaswami, V.) “Using Pyrene Fluorescence to Study
Conformation of Macrophage-Generated Nascent HDL Containing Human Apolipoprotein E CT”, poster # 339
25th Annual CSU Student Research Competition at Fresno, Oral presentation, May 6-7, 2011 (Bains, G., and
Narayanaswami, V.) “Developing Pyrene Fluorescence as a Powerful Probe to Study Protein Conformation: A New Aspect
of an Old Tool”
23rd Annual CSULB Student Research Competition, Oral presentation, Feb. 25th 2011 (Bains, G., and
Narayanaswami, V.) “Developing Pyrene Fluorescence as a Powerful Probe to Study Protein Conformation: A New Aspect
of an Old Tool”. (Secured second place in the competition in Biological and agricultural sciences)
23rd Annual CSU Biotechnology Symposium, Don Eden Award Finalist Oral and Poster Presentation, Jan. 7-8
2011, Hyatt Regency Orange County, CA (Bains, G., Patel, A.P., Narayanaswami, V.) “Developing Pyrene Fluorescence as
a Powerful Probe to Study Protein Conformation”, poster # 218
Society for Neuroscience Annual Meeting, Nov. 2010, San Diego, CA (Bains, G., Patel, A. B. and
Narayanaswami, V.) “Conformational Analysis of Apolipoprotein E using Pyrene Fluorescence as a Tool”, poster # 248.3
CSULB College of Natural Sciences and Mathematics Fall 2010 Student Research Symposium, Long Beach, CA
(Bains, G., Patel, A.P., Tien, T. Vu., and Narayanaswami, V) “Insight into the structure of lipid free Apolipoprotein E using
Pyrene, a spatially sensitive fluorescent probe”, poster # 3
Publications
Review Article – Bains, G*., Patel, A., Narayanaswami, V., “A Probe to Study Protein Conformation and
Conformational Changes, MDPI, 2011”.
Research Article - Bains, G*., Sea, Kim., Sorin, E.J., Narayanaswami, V “Extent of Pyrene Excimer
Fluorescence Emission is a Reflector of Distance and Flexibility: Analysis of the Segment Linking the LDL Receptor-
binding and Tetramerization Domains of Apolipoprotein E3”, Biochemistry, 2012.
Status
Permanent resident
* Gursharan Bains (Maiden name)
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