Quality Control Medical

Cover Letter

I have always been fascinated by the complexity of biology. My interest in biology and

technology persuaded me to choose clinical laboratory science as my field of bachelors. By

opting clinical laboratory technology, I became an expert in multidisciplinary labs and I also got

knowledge about the concept of quality control in a laboratory.

In M.Sc Medical Technology, my major was Molecular Biology and Cytogenetics. So I got

understanding of molecular biology and experienced the techniques of c onventional

cytogenetics. My thesis in the master was a research entitled "Phenotype and Genotype

Characterization of Eunuchs in Lahore".

After M.Sc, I contributed in a research project about circadian rhythm entitled “Expression of

Circadian Clock and Cell Cycle Genes in Chronic Lymphocytic Leukemia” at the Molecular

Biology Lab, University of Health Sciences Lahore, Pakistan. In those six months of extensive

research, I performed more than sixteen hundred Real Time Polymerase Chain Reactions. I

faced repeated calibration issues and performed quality control protocols there. This research

gave me expertise in basic molecular techniques such as DNA extraction, RNA isolation,

electrophoresis etc.

In addition, I worked as Laboratory Technologist/ Manager for the Advance Diagnostic and

Research Laboratory, University of Gujrat, Pakistan. The hospital associated with the laboratory

was serving thousands of patients with different genetic and infectious diseases. So I gained

experience with molecular-level analysis of diseases.

Currently I have almost completed MS Science and Bioengineering (Biotechnology as major

and Computational System Biology as minor studies) from Tampere University of Technology,

Finland. Presently I am writing the thesis manuscript which will hopefully end by couple of days.

My thesis was related to the fluorescent KillerRed protein and in the research, a manipulated

KillerRed protein was tested for its degradation abilities upon excitation . From my research

thesis, I also got introduced with molecular cloning. I know how to insert a DNA into an

expression vector and later transform competent cells to produce protein of interest. I also

know how to engineer (design) and produce phages. Moreover I have learned modern

biotechnology and I have expertise in the finest molecular techniques.

My masters in Finland have really given me enough confidence to work independently in a

research lab. I have practical experience in experiment planning, experiments execution (under

strict controls) and critical analysis of the results. I would like to widen and deepen my

knowledge and experience of the field by joining your team and I hope that you will provide me

an opportunity.

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Curriculum Vitae

Name Munawar, Mustafa Ahmad

Address Vaajakatu 5, B-26, 33720, Tampere, Finland

Cell +358*********

Email **************@*******.***,

*******.*******@***.**

Education:

Sr. Certification / Institution / Specialization / GPA / Passing

Degree University Major Marks Year

1. M.S Science Tampere Biotechnology as March/

and University of Major and April 2014

4 plus

Bioengineering Technology Computational

System Biology as

Minor Studies

2. M.Sc. University of Molecular Biology 2010

Health Sciences and Cytogenetics

Medical 960/1200

Lahore, Pakistan

Technology

3. B.Sc. in Medical University of Medical laboratory 2008

Laboratory Health Sciences Technology 1538/2200

Technology Lahore Subjects

Publication

Rana S, Munawar M, Shahid A, Malik M, Ullah H, Fatima W, Mohsin S, Mahmood S.

Deregulated expression of circadian clock and clock-controlled cell cycle genes in

chronic lymphocytic leukemia. Mol Biol Rep. 2014 Jan;41(1):95-103. doi:

10.1007/s11033-013-2841-7. Epub 2013 Nov 5.

Other Research Work

Developing biological tools to degrade lignocellulose (Thesis, MS Science and Bioeng.)

Phenotype and genotype characterization of eunuchs in Lahore. (Thesis, M.Sc Medical

Technology)

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Laboratory Expertise

Polymerase Chain Reaction (conventional and real time PCR)

DNA Recombinant Technology/ Molecular Cloning (Bacterial transformation with

recombinant plasmids)

Purification of His-tagged Proteins by Immobilized Metal Affinity

Chromatography (IMAC)

Purification of Proteins by Tri-phase Method

Preparation of Recombinant Plasmids

Competent Cell Preparation

Phage Stock Preparation

Protein and DNA Electrophoresis

Enzyme-Linked Immunosorbent Assay (ELISA)

Human Lymphocyte Culture & Chromosome Slide Preparation

Diagnostics; Serum Chemistry, Laboratory Haematology, Microbiology, Immunology etc.

All Kind of Solution preparations

Laboratory Math

Quality Control in Molecular Biology Lab

Laboratory Waste and Hazards Management

Experiences and Training

Job title Name of Institute Duration Year

Research Assistant Chemistry and Bioengineering Department, 6 months 2013

Tampere University of Technology, Finland

Laboratory ‘Advance Diagnostic & Research Lab’, Nawaz 8 months 2011-

Technologist/ Manager Sharif Medical College, University of Gujrat, 2012

Pakistan

Research Assistant with Department of Physiology and Cell Biology,

a PhD fellow University of Health Sciences, Lahore, 6 months 2011

Pakistan

Trainee Molecular Department of Human Genetics and

Technologists Molecular Biology,University of Health 1 year 2010

Sciences, Lahore, Pakistan

Trainee Laboratory Cytogenetics and Molecular Biology Lab, The

Technologist Children Hospital, Lahore, Pakistan 3 months 2010

Trainee Medical Rotation in Research Labs (Histopathology,

Technologist Immunology, Chemical Pathology, 1 year 2009

Hematology and Microbiology), University of

Health Sciences, Lahore, Pakistan

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Computer and Language Skills:

Proficient user of MS Word, Power Point and Excel.

Basics of MATLAB

Fluent in spoken and written English

Basic suomi (Passed Level 1 & 2 from Tampere University of Technology)

Social Membership & Co-curricular Activities:

Participation in relief medical camping during ‘Swat Army Operation 2009’, Pakistan.

Fund raising in Lahore during Swat Operation 2009, Pakistan

Volunteer participation in Pakistan Anti-polio Campaign 2004 and 2005.

References

References available upon request

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