University Research
Resume:
Name: Jieyan Pan
Degree: PhD, DVM
Sex: Male
Citizenship: China
Visa status: Permanent resident of USA
Home address:
Media, PA 19063
Work Address:
**** ***** Center Blvd Rm 1205G
The Children's Hospital of Philadelphia
Philadelphia, PA 19104
Contact information:
Tel: 215-***-**** (work)
215-***-**** (home)
Email: ****@*****.****.***
*********@*****.***
Education and Degrees:
July 2001: PhD in Veterinary Microbiology and Infectious Diseases
Key Laboratory of Animal Diseases Diagnosis & Immunity, Ministry of
Agriculture, Nanjing Agricultural University, China.
Advisor: Prof Puyan Chen
Title of Doctoral dissertation: Cloning of the Structural Protein Genes of
Avian
Infectious Bronchitis Virus and Study of their Roles in Pathogenesis
July 1996: DVM, Veterinary Medicine
School of Veterinary Medicine, Nanjing Agricultural University, Nanjing,
China.
Work experience and positions:
December 2013- now, Research Associate, The Children's Hospital of
Philadelphia
Research area: Interaction of coxsackievirus B3 with its receptors.
December 2008-November 2013, Postdoctoral Fellow, The Children's Hospital
of Philadelphia
Research area: 1) A murine oral infection model of human coxsackievirus B3;
2) Interaction of coxsackievirus B3 with its receptors.
April 2004-November 2008, Research Associate, University of Pennsylvania.
Research area: the function of genes related to mouse spermatogenesis,
specifically, Rnf17, Nxf2 and Nxf3
March 2003-March 2004, Postdoctoral Fellow, University of Pennsylvania.
Research area: the function of genes related to mouse spermatogenesis,
especially on mouse spermiogenesis;
March 2002-March 2003, Postdoctoral Fellow, University of Utah,
Research area: 1) Function of ELOVL4 using transgenic mice;
2) Positional cloning of gene(s) causing human North Carolina Macular
Degeneration (NCMD).
July 2001-March 2002, Postdoctoral fellow, Shanghai Institute of
Biochemistry and Cell Biology, Chinese Academy of Sciences.
Research area: Human antibody transgenic mice;
Publications:
1) Yoder JD, Cifuente JO, Pan J, Bergelson JM, Hafenstein S. The crystal
structure of a coxsackievirus B3-RD variant and a refined 9-angstrom cryo-
electron microscopy reconstruction of the virus complexed with decay-
accelerating factor (DAF) provide a new footprint of DAF on the virus
surface, J Virol. 2012 Dec; 86(23): 12571-81
2) Pan J, Narayanan B, Shah S, Yoder JD, Cifuente JO, Hafenstein S,
Bergelson JM. Single amino acid changes in the virus capsid permit
coxsackievirus B3 to bind decay-accelerating factor. J Virol. 2011
Jul;85(14):7436-43
3) Zhou J, Pan J, Eckardt S, Leu NA, McLaughlin KJ, Wang PJ. Nxf3 is
expressed in Sertoli cells, but is dispensable for spermatogenesis. Mol
Reprod Dev. 2011 Apr;78(4):241-9.
4) Pan J, Eckardt S, Leu NA, Buffone MG, Zhou J, Gerton GL, McLaughlin KJ,
Wang PJ. Inactivation of Nxf2 causes defects in male meiosis and age-
dependent depletion of spermatogonia. Dev Biol. 2009 Jun 1;330(1):167-74.
5) Newell AE, Fiedler SE, Ruan JM, Pan J, Wang PJ, Deininger J, Corless
CL, Carr DW. Protein kinase A RII-like (R2D2) proteins exhibit differential
localization and AKAP interaction. Cell Motil Cytoskeleton. 2008
Jul;65(7):539-52.
6) Wang PJ, Pan J. The role of spermatogonially expressed germ cell-
specific genes in mammalian meiosis.Chromosome Res. 2007;15(5):623-32.
7) Pan J, Goodheart M, Chuma S, Nakatsuji N, Page DC, Wang PJ. RNF17, a
component of the mammalian germ cell nuage, is essential for
spermiogenesis. Development. 2005 Sep;132(18):4029-39.
8) Pan J, Chen D, Chen P etal, Cloning and sequence analysis of the
membrane protein gene and 5a, 5b genes from avian infectious bronchitis
virus proventriculus strain isolated in Qingdao China, Chinese Journal of
Veterinary Science, 2002, 22 (2): 108-110;
9) Pan J, Chen D, Dai Y etal, Sequencing and sequence analysis of the S2
gene of infectious bronchitis virus proventriculus strain isolated in
Qingdao, China, Chinese Journal of Virology, 2001, 17 (4): 356-359;
10) Pan J, Chen D, Dai Y etal, Sequencing and sequence Analysis of the
nucleocapsid gene from infectious bronchitis virus (proventriculus strain)
(SD/97/02) isolated in Qingdao, Journal of Agricultural Biotechnology,
2001, 9 (4): 365;
11) Pan J, Chen D, Dai Y etal, Sequencing and sequence analysis of S1 spike
protein gene from avian infectious bronchitis virus proventriculus strain
isolated in Qingdao (SD/97/02), Virologica Sinica, 2001, 16 (4): 377-381;
12) Pan J, Chen D, Chen P et al, Cloning and identification of nucleocapsid
protein gene from avian infectious bronchitis virus proventriculus strain
isolated in Qingdao, Journal of Nanjing Agri Univ, 2000, 23 (3): 118-120;
13) Pan J, Chen D, Dai Y etal, Cloning and identification of S1 spike
protein gene from avian infectious bronchitis virus proventriculus strain
isolated in Qingdao(QXIBV), Chinese Journal of Veterinary Science and
Technology, 2000, 30 (11):7-9.
Manuscripts in preparation:
1) Pan J, Zhang L, Shen L, Turner JR, Bergelson. Expression of human
decay-accelerating factor (hDAF) on intestinal epithelium of transgenic
mice does not facilitate coxsackievirus B3 infection by the enteral route.
2) Pan J, Hafenstein S, Bergelson JM. A single amino acid residue in
murine DAF SCR2 prevents interaction with coxsackievirus B3.
Technical Skills
Recombinant DNA technology: molecular cloning; site-directed mutagenesis;
northern and Southern blot analysis; transgene expression in eukaryotic and
prokaryotic cells; in situ hybridization; reverse-transcriptase PCR;
quantitative real-time PCR; yeast genetics, including YAC cloning
Cell culture and virology: culture of cell lines and primary cells; virus
preparation and purification; radiolabeling; virus quantification by plaque
assay and PCR; generation of infectious cDNA clones; generation and
analysis of viral mutants; using SiRNA to test inhibition of virus entry
Mouse genetics: I have multiple years of experience on mouse genetics and
mouse model for virus infection.
Immunology: generation of monoclonal antibodies by hybridoma fusion;
generation and analysis of polyclonal antisera; antibody purification;
immunoblotting; ELISA
Protein production and analysis: generation of recombinant proteins in
bacteria and yeast; purification and characterization of recombinant
proteins
Pathology and imaging: specimen preparation including embedding and
sectioning; histochemistry and immunohistochemistry; fluorescence and
confocal microscopy
Computer: use of bioinformatics tools for DNA analysis and PCR; standard
office and presentation software (Microsoft Office; Photoshop; Illustrator;
Acrobat; Prism)
References:
Jeffrey M. Bergelson, MD
Professor of Pediatrics
The Children's Hospital of Philadelphia
University of Pennsylvania
Philadelphia, PA 19104
Phone: 215-***-****
Fax: 215-***-****
Email: *********@*****.****.***
P.Jeremy Wang, MD Ph.D
Professor of Developmental Biology
Director, Center for Animal Transgenesis and Germ Cell Research
School of Veterinary Medicine
University of Pennsylvania
Philadelphia, PA 19104
Phone: 215-***-****
Fax: 215-***-****
Email: *****@***.*****.***
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