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Medical Project

Location:
Media, PA
Posted:
February 26, 2014

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Resume:

Lin Yang, Ph.D in Molecular and Cellular Biology

Address: ** ********* **, *****, ** 19087

Tel. 610-***-****

E mail: l accvac@r.postjobfree.com.

Citizenship: USA

Q ua l if ications Summa ry

• Ph.D. in Cellular and Molecular Biology. Over ten years of research experience in

i mmunology and inflammatory diseases, with 6 years in academic institutions and 7 years

i n pharmaceutical indust ry.

• Deep knowledge in cellular and molecular immune process in in flammation and oncology.

• Skilled in cellular and molecular techniques, including cell culture, multi-color f low

cytometry, cell imaging (epi-f luorescent microscopy and confocal microscopy for live or fixed

cells), siRNA to know down protein expression, EL ISA, western blot, SDS-PAGE,

i mmunoprecipitation, PCR, microarray, DNA construction and t ransfection into cells,

i mmunostaining, automation and working with animal models.

• Strong ability to independently design and carryout experiments.

• St rong ability to analyze data, interpret and present results. Excellent wri t ten and verbal

communication skills.

• Four year experience of supervisor and project leader.

• Strong ability to wri te research proposals, publication manuscripts and data

summaries/conclusions/interpretation .

• Extensive experience of collaboration with CRO or scientific institutions.

• Easy going. A good team player.

E ducation

Doctor of Philosophy, Cellular and Molecular Biology, University of Sheffield, UK 2002

M aster of Science, Pharmacology, Guiyang Medical College, China 1996

M .D., Guiyang Medical College, China 1989

O ther t raining:

• Drug development t raining (Legacy Wyeth Pharmaceuticals, USA) 2008

• Project management (American Management Association, USA) 2008

• International Business Communication (University of Pennsylvania, USA) 2010

P rofessional Experience

05/2013-present: Consultant, I magingCells, Philadelphia, PA.

P roviding consulting service for biotech and pharmaceutical companies, focusing on assay

development, biomarker identification, cellular & molecular techniques and experiment design in

t he area of inflammation, immunity, oncology and obesity/diabetes.

09/2006-05/2013: Senior Research Scientist, Pfizer, Collegeville, PA

• Leading a Cell Immunology lab to develop in vit ro and in vivo assays for determination of

t herapeutic effects of small and large molecules on anti-inflammation, anti-infection, cell proliferation,

epithelial bar rier function, drug bioavailability and identifying biomarkers. Ini tiating ideas, supervising

technicians, writ ing protocols, t rouble shooting and method validation.

• Establishing and leading a Cell Imaging facility.

• Technologies: DNA construction, siRNA t reatment, DNA t ransfection, immune cell isolation and

quantitation using f low cytometry, ELISA, SDS PAGE, Western blot, immunostaining, Luminex,

Cellnomics, f luorescent microscopy, imaging quanti tation, PCR, siRNA, genechip, automation, gene

analysis and profiling using Ingenuity program, animal model establishing and cell isolation from blood

and t issues.

• Working with cell culture, animal t issue and clinical samples.

• Analyzing data, interpreting and presenting results, writ ing research summaries and drafts for

documentation and publication.

• Project leader: hosting regular meetings and monitor project progress, ensuring efficiency

and on t imeline targets, prioritizing tasks.

• Cross working with manufacture analytical, clinical, medical and regulatory groups.

• Establishing and managing collaborations with external partners.

07/2002-09/2006: Postdoctoral fellow, Division of Vascular Research, Department of

P athology, Ha rvard Medical School and Brigham & Women’s Hospital.

• Investigating the interaction of endothelial cells and inflammatory cells, and signaling

pathways in endothelial cells that cause inflammatory diseases and vascular dysfunction.

• Using blocking antibodies, mutated cDNA or siRNA to identify a novel signaling pathway

i n endothelial cells that regulates inflammatory cell adhesion and t ransmigration.

• Writing manuscripts for publication.

08/1998-06/2002: Doctoral student, Cellular and Molecular Biology, University of Sheffield,

UK

Dissertation entitled “Regulation of I κB α-Quanti tative single cell analysis”

Investigating the role of I kappa B alpha in regulation of NF-kappa B signal t ransduction pathway

u pon cytokine stimulation in both normal and cancer cells.

Techniques: plasmid construction, mutation of cDNA, t ransfection of plasmids, immunostaining,

i mmunoprecipitation, western blot, f luorescent microscopy, live cell imaging and FRET.

05/1997-05/1998: Research fellow, Sheffield Kidney I nstitute, Northern General Hospital,

U niversity of Sheffield, UK.

Investigating the role of the cytokines, IGF-1, TGF- β1, the protein α-SMA and the extracellular

mat r ix components collagen I I I and IV in kidneys of rats with diabetic nephritis and subtotal

nephrectomy. Techniques: immunohistochemistry and ELISA.

09/1996-08/1998: Lecturer, Dept. of Pharmacology, Guiyang Medical College, China.

• Teaching Clinical Pharmacology.

• Investigating herb functions in cancer therapy using in vivo and in vit ro models.

09/1993-07/1996: Graduate Student, Ms. in Pharmacology, Guiyang Medical College, China.

Dissertation entitled “ Therapeutic effect of a Chinese Medicine (Cili, or Rosa roxhurthii Tratt )

enriched with superoxide dismutase (SOD) on m ice and rats with lead poisoning”

09/1989-08/1993: physician, Guiyang Medical College Affiliate Hospital, China.

Professional Societies

A merican Society for Investigative Pathology. 2002 - present

A merican Society for Nut r i t ion. 2006 - present

A merican Society for Microbiology 2002 - present

A merican Society for Cell Biology 2010 - present

Selected Publications

Paper publications:

1. Lin Yang, Hongbin Chen, and Eva E Qwarnstrom. Degradation of I kBa is limited by a post

phosphorylation/ubiquitination event. B iochemical and Biophysical Research Communications, 2001. 285, 603-

608.

2. Kehinde Ross, L in Yang, Steve Dower, Filippo Volpe and Francois Guesdon. Identification of Threonine 66 as

a F unctionally Critical Residue of the Interleukin-1 Receptor-associated Kinase. J ourna l of Biology Chemistry,

2002. 277, 374**-*****.

3. Lin Yang, Kehinde Rose and Eva E Qwarnstrom. RelA control of I kBa Phosphorylation - A positive feedback

loop for high affinity NF-kB complexes. J ourna l of Biology Chemistry, 2003. 278(33), 308**-*****.

4. Ganguli A, Persson L, Palmer IR, Evans I, Yang L, Smallwood R, Black R, Qwarnstrom EE.. D istinct NF-

kappaB regulation by shear stress through ras-dependent I kappaBalpha oscillations: real-time analysis of f low-

mediated activation in live cells.Ci rculation Research, 2004, April, 96(6):626-34.

5. Yuan Liu, Sunil K. Shaw, Shuo Ma, L in Yang, F. William Luscinskas, Charles A. Parkos. Regulation of

leukocyte t ransmigration: cell surface interactions and signaling events. J ourna l of I mmunology. 2004 Jan

1;172(1):7-13.

6. Shaw SK, Ma S, Kim MB, Rao RM, Hartman CU, Froio RM, Yang L, Jones T, Liu Y, Nusrat A, Parkos CA,

L uscinskas FW.Coordinated redistribution of leukocyte LFA-1 and endothelial cell ICAM-1 accompany neutrophil

t ransmigration. J ourna l of Experimental Medicine . 2004 Dec 20;200(12):1571-80.

7. Lin Yang, Richard Froio, Tracey Sciuto, Ann M Dvorak, Ronen Alon and F. William Luscinskas. ICAM-1

regulates neutrophil adhesion and t ranscellular migration of TNF-alpha-activated vascular endothelium under

f low. B lood. 2005 Jul 15;106(2):584-92.

8. Lin Yang, Jennifer R. Kowalski, Xi Zhan, Sheila M. Thomas and Francis W. Luscinskas. Endothelial cell

cortactin phosphorylation by Src contributes to polymorphonuclear leukocyte t ransmigration in vitro. C i rcul ation

Research, 2006 Feb 17;98(3):394-402.

9. Lin Yang, Jennifer R. Kowalski, Patrick Yacono, Milan Bajmoczi, Sunil K. Shaw, Richard M. Froio, David E.

Golan, Sheila M. Thomas, and Francis W. Luscinskas. E ndothelial Cell Cortactin Coordinates Intercellular Adhesion

Molecule-1 Clustering and Actin Cytoskeleton Remodeling during Polymorphonuclear Leukocyte Adhesion and

T ransmigration . J ourna l of I mmunology,2006; 177: 6440 - 6449.

10. Sircar M, Bradfield PF, Aurrand-Lions M, Fish RJ, Alcaide P, Yang L, Newton G, Lamont D, Sehrawat S,

Mayadas T, Liang TW, Parkos CA, Imhof BA, Luscinskas FW. Neutrophil transmigration under shear f low conditions

i n vitro is junctional adhesion molecule-C independent. . J ourna l of I mmunology. 2007 May 1;178(9):5879-87.

11. Rao RM, Yang L, Garcia-Cardena G, Luscinskas FW. Endothelial-dependent mechanisms of leukocyte

recruitment to the vascular wall. . C i rculation Research 2007 Aug 3;101(3):234-47.

Abstracts:

1. Lin Yang, F ranco Carlotti, and Eva E Qwarnstrom. Preferential Degradation Of I kBa Associated With NF-kB

B ut Reassociation Of NF-kB With Free I kBa Before Nuclear Translocation. Glasgow, UK. T he Biochemical Society

T ransactions. August 1999 27(4):94.

2. Lin Yang and Eva E Qwarnstrom. Regulation of I kBa through phosphrylation- dependent high-affinity NF-

kB interactions. 2001, Trinity College, Dublin, I reland. T he Biochemical Society Transactions. November 2001,

29(6):133. (Awarded as the Fi rst P rize for Posters )

3. Lin Yang, Richard Froio, Tracey Sciuto, Ann M Dvorak, Ronen Alon and F. William Luscinskas. ICAM-1

regulates neutrophil adhesion and t ranscellular migration of TNF-{alpha} activated vascular endothelium under f low.

New Mexico, USA. Keystone Symposia. 2005, 79.

4. Lin Yang, Jennifer R. Kowalski, Xi Zhan, Sheila M. Thomas and Francis W. Luscinskas. Endothelial cell

cortactin phosphorylation by Src contributes to polymorphonuclear leukocyte t ransmigration in vitro. San Francisco,

USA. Experimental Biology. 2006. April. 1-5. (Oral presentation )

5. Lin Yang and Martin Kullen . Q uantitative Evaluation of Enteropathogenic Escherichia coli Adhesion to

Epithelial Monolayersin vitro. Boston, USA. A merican Society for M icrobiology 108 General Meeting . 2008.

th

6. Lin Yang, Heidi Emerich and M artin Kullen. Effect of Alpha-Lactalbumin and Alpha-Lactalbumin-Derivd

Peptides on the Adhesion of Diarrheagenic Bacteria to Caco-2 Monolayers. A merican Society for Cell Biology 50th

A nnual Meeting 2010.



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