PURASKAR INGALE

+1-210-***-**** **** Catamaran St. Apt#3, Foster City, CA-94404 accpqv@r.postjobfree.com

“An Innovative, flexible, resourceful team member; contributing to the growth of the company is looking for a challenging

career with immense possibilities for development in a firm.”

EDUCATION

Aug.2009-Dec.2011 M.S. Biotechnology The University of Texas at San Antonio, San Antonio, TX GPA 4.0

Aug.2004-July 2008 B.Engg. Biotechnology Shivaji University, Kolhapur, India GPA 3.88

Coursework: Biochemistry, Cell and Molecular Biology, Immunology, Bioinformatics, Biostatistics, Protein Engineering,

Advanced genetic engineering, Biology of Diseases, Biotechnology Laboratory, Protein Purification Laboratory, Enzyme

Technology, Fermentation Technology, Bioreactor, Bioprocess Engineering, Microbiology

RESEARCH EXPERIENCE

June 2013-Present Research Fellow(Visiting) Biomedical Research Foundation of South Texas, San Antonio, TX

o Epigenomic and genomic editing via TALEN, TALEM and Cas9/CRISPR for research on personalized medicine

o Primary human cell culture, conditional reprogramming of cells, selection of KO single cell mutants, HR DNA repair

Feb.2012-Present Research Fellow Department of Medicine/Infectious diseases, The University of Texas

Health Science Center, San Antonio, TX

o Clinical research projects on HIV/AIDS host genetic & epigenetic determinants and allergy

o Maintaining lab inventory and ordering lab supplies and consumables; helping in writing, editing papers and grants

Dec.2009-Feb.2012 Research Assistant John McCarrey Lab -The University of Texas at San Antonio, San

Antonio, TX

o Extensive use of advanced molecular biology techniques to study allele -specific DNA methylation and allele-specific

expression in various tissues isolated from mice produced by cloning and ICSI

Dec.2004-Aug.2008 Research Assistant Tatyasaheb Kore Inst. of Engg. and Technology, Warananagar, India

o Member and leader of various research project teams

o Projects areas: Environment engineering, Molecular Biology, Manufacturing & design, Bioinformatics

May-Sept.2007 Internship(Industry) Serum Institute of India, Pune, India

o Project on ‘Standardization and validation of real time-PCR for residual DNA estimation from Biopharmaceuticals’ at

Research and Development Division under guidance of Dr. Manoj Kumar following GxP (GMP, GLP, etc.) and Six Sigma

principles; assisted in QC; handling biological fluids and animals

Dec.2005 Internship(Institute) National Environmental Engg. Research Institute, Nagpur, India

o Carried out various assays for analysis of different Phyto-remediation strategies and got satisfactory results for Mn2+,

Co2+ rich soils, plant tissue culture

RESEARCH PUBLICATIONS

1) de Waal EM, Yamazaki Y, Ingale PN, Bartolomei MS, Yanagimachi RY, McCarrey JR. (2012 Mar 13). Prima ry epimutations

introduced during intracytoplasmic sperm injection (ICSI) are corrected by germline -specific epigenetic reprogramming. Proc

Natl Acad Sci USA. 109(11):4163-8. PMID: 22371603.

2) de Waal EM, Yamazaki Y, Ingale PN, Bartolomei MS, Yanagimachi RY, McCarrey JR. (2012 Oct 15). Gonadotropin

stimulation contributes to an increased incidence of epimutations in ICSI -derived mice. Human Molecular Genetics.

21(20):4460-72. Epub 2012 Jul 16. PMID: 22802074.

3) Okulicz JF, Le TD, Agan BK, Camargo J, Landrum ML, Wright EJ, Dolan MJ, Genesan A, Perguson TM, Smith DM, Richman

DD, Carrillo A, Ingale P, Little SJ, Clark RA, He W, Ahuja SK. (In Press 2014 July). Influence of the Timing of Anti -retroviral

Therapy on Normalization of Immune Status in HIV-1 infected Individuals. JAMA-Int. Med. Manuscript#JAMA14-0655.

TECHNICAL SKILLS

o Molecular Biology Techniques: Isolation and characterization of DNA and RNA from different sources, transformation,

cloning, plasmid construction, plasmid sub-cloning, ligation, restriction digestion

o Advanced Molecular Biology Techniques: PCR, RT-PCR, real-time PCR, single-cell qPCR, gradient-PCR, bisulfite

treatment PCR, genotyping, DNA sequencing, sequence analysis, mutagenesis, KO creations, homologous recombination,

RNA interference, restriction mapping and RFLP analysis, primer designing, ChIP-Seq, RNA-Seq

o Cell and Tissue culture: Animal tissue culturing, passaging and characterization of adherent and suspension cell lines and

primary cells, bio-banking, plant tissue culture, stem cell culture, isolation of PBMCs from blood

o Radiochemical analysis: Skilled in use of 32P radioisotopes, Gamma irradiator

o Microscopy: Light, inverted, phase-contrast and fluorescence microscopy

o Biochemical Analysis: CHOPS ELISA, standardization of enzyme assays, gel electrophoresis [Vertical/ horizontal gel;

agarose/polyacrylamide(SDS PAGE)/urea], southern blotting, western blotting, northern blotting, FACS studies,

immunoblotting, immunoprecipitation, immunostaining

o Animal handling: Handled mice and rabbits, extraction and handling of biological fluids, in vivo tests

o Protein Purification: Experience in the purification and quantitation of antibodies, preparation of cell/tissue lysates, protein

A purification, SCX purification, anion exchange chromatography, affini ty chromatography, paper chromatography, thin layer

chromatography, gas chromatography

o Major Equipment used: PCR, Real time PCR, Biomark HD, 2100 Bioanalyzer, TECAN, BD FACSCalibur, AutoMACS,

QIACube, NanoString, ultracentrifuges, electrophoresis system, UV-VIS double beam spectrophotometer, flame photometer,

nanodrop spectrophotometer, cell cytometer, inverted/fluorescence/phase-contrast microscope, Kjeldahl’s apparatus, gel

dock, genetic analyzer, Illumina Hi-Seq 2000, BioRad versadoc imager, personal molecular imager FX, sonicators -

Diagenode bioruptor UCD300, Diagenode IP Star Compact, homogenizer, bead beater, bioreactor

COMPUTER SKILLS

o Diploma in Information Technology (DIT) from Centre for Development of Advanced Computing, Mumbai, INDIA

o Bioinformatics: Online Sequence Analysis: BLAST, multiple sequence alignment, ExPASy, Clustal X2, gene homology tools,

DS Gene, DS Viewer, Stratagene, Phoretix, Alpha Imager 2200 Documentation and imaging System Ver. 5.5, ABI PRISM

7000 Sequence Detection System Software Ver. 1.1, Bio-Rad Versa Doc Ver. 4.6.9, Finch TV Ver. 1.4.0, Serial Cloner 2.0,

GraphPad Prism, ICM Browser, Agilent GeneSpring GX, CLC Workbench 6.0.1

o Everyday computing -- OS: DOS, Windows 7/8, Mac OS X Lion; Programming language: C, HTML scripting; MS Office 2013

Enterprise suite, EndNote X6; Graphics Designing Software: Corel Draw 12, Adobe CS 2 suite, Inkscape 0.48.1

o Laboratory Information Management System (LIMS)

RESEARCH PROJECTS (Recent - in past 2 years)

Genomic and epigenomic editing using the site-directed Transcription Activator-like Effector Nucleases (TALEN), methylases

(TALEM) and Cas9/CRISPR system(Jan. 2012-Present) – PI: Ahuja S.K., M.D.

o Designed and created the TALEs, Cas9/CRISPR in the lab; Additional work includes the work on long non-coding RNA

molecules, generation of Conditionally Reprogrammed Cell lines (CRCs) from primary cells from humans, Biomarker

analysis of nasal secretions and plasma from the allergy clinical trials

RESEARCH PAPER PRESENTED - International, National Conferences (selected from 10 peer-reviewed papers)

1) “Biomonitoring of air around Warana industrial complex” by Ingale PN at ‘Third Regional symposium on environment &

natural resources’ - "Conservation For A Green Future" (5th & 6th August 2008) at Kuala Lumpur, Malaysia

2) “Production of streptokinase in batch culture” by Kamble RD, Ingale PN, Patil SA, and Deshmukh AM, at Global Sustainable

2nd Biotech Congress (18th-21st Dec. 2006) R.T.M. Nagpur University, Nagpur, Maharashtra, India, Pg. 293

3) “Fermentative production of bio hydrogen using activated sludge from dairy waste treatment plant as inoculum” by Ingale

PN, Mandape SN, Shah SA, Wanpal NS, Patil SA, Shejul MS and Pawar SH, at ‘ 3rd Convention of BRSI and International

Conference’ on “Exploring Horizons in Biotechnology: A Global Venture “(Nov. 2-4, 2006), Vallabh Vidyanagar, Gujarat,

India’, Pg. 166

4) “Antibody Directed Enzyme Prodrug Therapy” by Ingale PN, Mandape SN at “Parichay 2006”, K.L.E.S. College of Engg. &

Tech., Belgaum, Dist. Belgaum (Karnataka); at “Chemstorm 2006”, P.R.E.C., Pravaranagar, Loni, Dist. Ahmednagar (M.S.);

at “Eureka 06”, T.K.I.E.T., Warananagar, Dist. Kolhapur (M.S.), India

o 3rd prize at ‘Chemstorm 2006’.

TEACHING EXPERIENCE

Jan. 2010–Dec. 2011 Teaching Assistant II Genetics Lab, Biology Department, UTSA, San Antonio, TX

Duties: Teach at least two labs having 20 students/lab in each semester; Experiment set up and supervision of the new

graduate teaching assistants; Lecture on background information; Design and grading of quizzes and test; Personal

assistance to each student raising their grades by at least one letter grade point

PROFESSIONAL AFFILIATIONS

Executive Board Advisor (2011-14) and President (2010-11) – Indian Students’ Association at UTSA; Secretary (2009-10) -

Bio Club at UTSA; Member (2009-11) – Public Relations Committee of UTSA Graduate Students’ Association (GSA);

Member (2009-11) – Latin Dance Society (LDS) at UTSA; Treasurer (2005-06) and Secretary (2007-08) – Biotechnology

Innovators’ and Technocrats’ Society (BITS) at TKIET; Students’ Council of TKIET (2006-07); ISTE’s student chapter at TKIET

(2005-06); Student in-charge for Eureka-Jidnyasa (National level technical competition, 2005-08), NCEEM-2008; Magazine

committee of TKIET (2004-06)

AWARDS AND HONORS

o Best Outgoing Biotechnologist-2008 of TKIET; 1st prize, Traditional skit (07), TKIET; 1st prize photography, TKIET;

Cultural general secretary of TKIET (2006-07); Head boy (2001-02), Sāndipani School

o Scholarships: TKIET scholarship (2004-2008), Maharashtra State Teachers’ Scholarship (2004-2008), Nagpur Municipal

Corporation Scholarship (2002-2008)

EXTRA-CURRICULAR ACTIVITIES

Lead a team of 20+ people in The City of San Antonio's International Affairs Dept. organized Diwali Festival (2010-12)

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