Scientific person with experience in analytical and manufacturing.
Resume:
ROHAN KAUSHAL Email:
********@*****.***
Lexington, KY 40508
SUMMARY:
Biomedical engineer with a 6+ years of global experience in research, data analysis and manufacturing of biopharmaceuticals. A unique and demonstrated background, spanning over multiple disciplines such as molecular & cell biology, data analysis and management, behavioral neuroscience, pain research (in vivo models), tissue culture & histology, protein analytics, neurosurgery, pharmacology and analytical toxicology.
EDUCATIONAL BACKGROUND:
. University of Kentucky
Master of Science (M.S.), Medical Sciences GPA
3.48/4.0
Aug, 2009-Dec, 2013
. University at Buffalo, The State University of New York
Master of Science (M.S.), Biotechnology GPA
3.45/4.0
Aug, 2007-Sept, 2009
. Kurukshetra University
Bachelor of Technology (B.Tech), Biotechnology
GPA 3.87/4.0 Aug, 2002-June, 2006 (First class, Honors)
RESEARCH PROJECTS:
. Worked on, "Studying the role of locus coeruleus (A6) noradrenergic
neurons in the facilitation of chronic orofacial neuropathic pain" at
University of Kentucky
. Worked on, "Reconstruction of the nigrostriatal dopamine pathway using
axon regeneration and embryonic tissue micro transplantation in an
adult parkinsonian rat" at University of Kentucky
. Worked on, "Optimization and Standardization for the low level
detection of polybrominated diphenyl ethers (PBDE's) using GC-ECD" at
Toxicology Research Center, SUNY At Buffalo
RESEARCH SKILLS & LABORATORY TECHNIQUES:
. Instrumentation: Calibration and conditioning of GC columns,
acquainted with extraction protocols and installation of GC columns,
Operating GC-ECD & GC-MS, pH Meter, spectrophotometry, NanoDrop, HPLC,
TLC, ION-Exchange chromatographic unit, Light microscope, Phase
contrast microscope, Fluorescent microscope
. Surgeries: Infra orbital chronic constriction injury (ION-CCI),
Stereotaxic surgeries for pathway reconstruction, Cranial cannula
implantation, Tissue transplantation, drug delivery using infusion
pumps through cannula, perfusion and organ harvesting.
. Rodent Behavior: Von frey fiber testing, open field testing, light
dark box, Hot & Cold plate testing, cylinder test
. Molecular Biology: Lentivirus and AAV production, PCR, Western
blotting and quantitative analysis using LICOR-Odyssey, SDS-PAGE,
Agarose gel electrophoresis and Gel analysis, RNA extraction, DNA and
plasmid isolation and quantitation, Gene cloning, Transformation,
Protein expression, isolation and purification
. Immunology: ELISA, Immunohistochemistry (IHC), Cell counting, Tissue
Cell culture
INDUSTRY WORK EXPERIENCE:
Organization Period
Position
Panacea Biotec. Ltd., India Aug, 06 - July, 07 Tr.
Scientific Officer
. Established a state of art vaccines manufacturing facility
. Prepared and revised user required specification, design
qualification, standard operating protocol and batch production
records
. Installed and commissioned various equipments for filling and
formulation of vaccines
Orchid Biomedical, Goa, India Summer 2005 Engr.
Intern
. Manufacturing of rapid diagnostic kits like HIV (ELISA Kits), Malaria,
Hepatitis etc
Alembic Pharmaceuticals, India Summer 2004 Engr.
Intern
. Strain improvement of S. erythraeus and antibiotic production
(Erythromycin) at pilot plant level
AWARDS AND HONORS:
2012 Travel award for Society for Neuroscience (SFN), 2012 by Graduate
School and Department of Physiology, UK, USA
2012 Kentucky Opportunity Fellowship for the 2012-2013 academic year
2009 Kentucky Opportunity Fellowship for the 2009-2010 academic year
PUBLICATIONS:
Master Thesis: Optimization and standardization of GC-muECD method for the
low level determination of seven polybrominated diphenyl ethers (PBDEs)
found in serum sample.
http://gradworks.umi.com/14/69/1469091.html
Kaushal, R., Zhang, L., Taylor, B.K., and Westlund, K. N. (In Preparation).
Selective Elimination of Locus Coeruleus Noradrenergic Neurons Alleviates
Chronic Orofacial Pain.
POSTER PRESENTATIONS:
*R.H. Kline IV, *R. Kaushal, A. Jamal, K.N. Westlund (2013) Chronic
constriction injury of the infraorbital nerve produces anxiety-like
behaviors in the rat
8th Annual CCTS Spring Conference University of Kentucky Center for
Clinical and Translational Science Monday, April 8, 2013
*equal contributing authors
Rohan Kaushal, Fei Ma, Liping Zhang, Bradley K. Taylor and Karin N.
Westlund (2012)
Knockdown of Locus Coeruleus Noradrenergic Neurons Alleviates Chronic
Orofacial Pain
22nd Annual Neuroscience Day, Louisville Chapter, April 19, 2012
Rohan Kaushal, Fei Ma, Liping Zhang, Bradley K. Taylor and Karin N.
Westlund (2012)
Knockdown of Locus Coeruleus Noradrenergic Neurons Alleviates Chronic
Orofacial Pain
18th Annual Kentucky Spinal Cord & Head Injury Research Trust (KSCHIRT)
Symposium May 11, 2012
Rohan Kaushal, Ahmad Jamal, Liping Zhang, Bradley K. Taylor and Karin N.
Westlund (2012)
Knockdown of Locus Coeruleus Noradrenergic Neurons Alleviates Chronic
Orofacial Pain
Society for Neuroscience, 2012
COMPUTER SKILLS:
. Graph Pad Prism6, MS Word, Excel, End note, Power Point, Adobe
Photoshop, SPSS
Data analysis
. Well acclimatized with NCBI, PubMed and other Bioinformatics software
viz. RasMol, Cn3D, Swiss-PDB, Clustal W, GenScan and NebCutter,
sequence alignment using BLAST and FASTA
STRENGHTS:
. Expertise in stereotaxic brain surgeries and behavior testing
. Experience in the field of manufacturing of pharmaceuticals in general
and vaccines
. Experience in customer service
. Presentation skills and quick learning capability
PROFESSIONAL SOCIETY MEMBERSHIPS:
2009-Current Member: Bluegrass Chapter of the Society for Neuroscience
(BGSFN), UK
2012 Member: Louisville Chapter-Society for Neuroscience, UL
2012 Member: Society for Neuroscience (SFN)
ADDITIONAL TRAINING:
. Attended 10 day workshop on Immunodiagnostics organized Dr. Subbarao
Gunupuddi, Owner and Director, Dxsys Systems, San Francisco
. Attended 3 week training on "Role of Biotechnology in Aquaculture"
under supervision of Dr. Janardan Jee at National Research Centre for
Makhana, ICAR- Darbhanga, India
REFERENCES
. Available on request
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