University Project
Resume:
CURRICULUM VITAE
S/o Shashigangadhar
SHIVAVEERAKUMAR S.
H. No. 10-2/58,
Sangameshwar Colony,
Gulbarga – 585 103
Mobile No.: +91-998*******
E-mail: *************@**********.***
Objective:
To be associated with an Organization/Institution where my knowledge
and skills are utilized and which provides an opportunity to enhance my
knowledge and skills.
ACADEMIC PROFILE:
Thesis on “Production of tyrosinase by actinomycetes and their molecular
characterization with metagenomic attributes” for the award of Doctor of
Philosophy degree on 15.02.2014
During Ph.D.: Selected for CIMO International Research Fellowship
Since Aug. 2011 to Mar. 2012 Working at Department of Environmental Sciences,
Helsinki University, Niemenkatu 73, Lahti – 14150, Finland
Ph. D. in Microbiology (Registered in 2008)
Registration in 2008
Department of Microbiology, Gulbarga University, Gulbarga.
Thesis Entitled: “Production of tyrosinase by actinomycetes and their molecular
characterization with metagenomic attributes”
Research Guide: Dr. Dayanand Agsar, Professor of Microbiology.
M. Sc. in Microbiology (2005-2007)
Department of Microbiology, Gulbarga University, Gulbarga.
Class: 67.75% Aggregate
B. Sc. Integrated Biotechnology (2002-2005)
Vishveshwariah College of Applied Science, Gulbarga.
Class: 78.92% Aggregate.
RESEARCH AND TEACHING PROFILE:
RESEARCH EXPERIENCE (5 years):
Completed project “‘Recent trends in actinobacterial diversity of compost
and their potential bioactive molecules’ with 16S rDNA and Metagenomic
approach by Pyrosequencing” funded by TEKES and National institute of
occupational health and Centre for International Mobility and cooperation,
Finland from August, 2011 to February, 2012.
Completed thesis of Ph.D. programme entitled: “Production of tyrosinase
by actinomycetes and their molecular characterization with metagenomic
attributes” under the guidance of Dr. Dayanand Agsar, Professor of
Microbiology Gulbarga University, Gulbarga since from May, 2008 to till
the date.
Completed project “Isolation, antibiogram and plasmid curing of
aetiological agents of urinary tract infection” under the guidance of Dr.
C. T. Shivannavar, Professor of Microbiology, Gulbarga University,
Gulbarga from April, 2007 to October, 2007.
Completed project on “Detection of Potential Proteolytic Microorganisms”
under the guidance of Dr. Sarvamangala R. Patil, Vishveshwariah College
of Applied Science, Gulbarga from March, 2004 to September, 2004.
TEACHING EXPERIENCE (2 years):
Since from May, 2008 involved in academic activities as theory and practical
classes for the Post Graduate (Microbiology) studies.
PAPERS ACCEPTED/ PUBLISHED:
S. Shivaveerakumar, D.N. Madhusudhan, H. Raghavendra, Dayanand
agsar, Screening of Streptomyces and process optimization for the
production of tyrosinase. Journal of Pure and Applied Microbiology. Vol 8,
No 8, 2013 (Accepted) (Impact factor: 0.065)
Mousumi das, S. Shivaveerakumar, Dayanand agsar, Extracellular
production of L-Glutaminase under submerged system by Streptomyces
rochei detected from the lime stone quarries of deccan trap. Journal of Pure
and Applied Microbiology (2013, Accepted) (Impact factor: 0.065)
S. Shivaveerakumar, D.N.Madhusudhan, Dayanand agsar, Merja Kontro,
Catalytic zone, a novel screening approach for the extracellular tyrosinase
by Streptomyces vinaceusdrappus DSV5. Journal of Pure and Applied
Microbiology. Vol 7, No.4, 2013 (Accepted) (Impact factor: 0.065)
“Production of L-Glutaminase by Pseudomonas VJ-6”. Hiremath Jyothi,
Shivaveerakumar, Rathod Vandana, Research Journal of Biotechnology, 42
– 49, Vol 6(3), August 2011 (Impact factor: 0.143)
CONFERENCES/WORKSHOP/SEMINAR PARTICIPATED
Participated in two days KSTA regional conference on Science and
technology for development organized by Central University of Karnataka,
School of Earth Sciences, on 30th and 31st January 2014.
Presented a poster in International conference on Advances in
Biotechnology and Bioinformatics entitled “Evaluation of upstream
bioprocessfor the enhanced production of tyrosinase by Streptomyces
vinceudrauppus” held at Dr. D.Y. Patil Vidyapeeth Pune from Nov. 25-27,
2013.
Participated in the National conference on Nanotechnology’s Invisible
Threat: Small Science, Big Consequences” organized by Mahatma Gandhi
National Institute of Research and Social Action (MGNIRSA), Hyderabad
from 26-27, September, 2013.
Attended one day seminar on Food Security and Natural Resources
Management sponsored by University Grants commission New Delhi,
organized by Faculty of Science and Technology Gulbarga University,
Gulbarga on 8th October, 2012.
Attended two days workshop on Research Methodology for Life Science
Research Students organized by Department of Biotechnology, Gulbarga
University, Gulbarga from 29th –30th May, 2012
Presented a poster in First National Conference on Recent Advances in
Nanobiotechnology entitled “Protoplast fusion in Streptomyces for
enhanced production of L-Glutaminase and Tyrosinase” held at Post
Graduate Department of Studies and Research in Biotechnology, Gulbarga
University, Gulbarga from 20th to 21th March, 2012
Participated in National Seminar on Environment Education for Sustainable
Development organized by Purushottam Walwalkar Higher Secondary
School, Mapusa in collaboration with Centre for Environment Education,
Goa from 25th to 27th November, 2010.
Participated in State Inter University Symposium (Biochemistry,
Biotechnology, Microbiology and Bioinformatics) sponsored by Vision
Group on Science and Technology, Department of Science and Technology,
Government of Karnataka, held at Gulbarga University, Gulbarga on 3rd
and 4th March, 2010.
Attended National Symposium on “Role of Biology and Biotechnology in
Conservation of Biodiversity and Sustainable Development” held at
Department of Botony, Gulbarga University, Gulbarga from 22nd – 24th
December 2009.
Presented a poster entitled “Production of Tyrosinase by novel isolates of
Streptomyces” organized by 50th Annunal Conference Association of
Microbiologist of India held at National Chemical Laboratory, Pune from
15th to 18th December 2009.
Attended seminar on “Advances in Nanotechnology” organized by
Vishweshwariah College of Applied Sciences, Gulbarga, Nov, 2009.
Attended second National conference on Science and technology organized
by Karnataka Science and Technology Academy and Gulbarga University
Gulbarga on Sept 23 – 25, 2009.
Participated in symposium “Antimicrobial Resistance” organized by
Sir Dorabji Tata Centre for Research in Tropical Diseases, held at
Department of Microbiology and Cell Biology, Indian Institute of Science,
Bangalore on March 10 – 11, 2008.
Presented a poster entitled “Isolation, antibiogram and plasmid curing of
aetiological agents of urinary tract infection” organized by 2nd National
Conference on Biotechnology for industrial and rural development
(NCBIRD 2008) held at Department of Biotechnology, Gulbarga University,
Gulbarga from 17th to 19th January 2008.
Attended the One Day Awareness Training Programme on Biodiversity
Related Issues and People’s Biodiversity Register, organized by Department
of Biotechnology, Gulbarga University, Gulbarga and Karnataka
Biodiversity Board, Bangalore held at Department of Biotechnology,
Gulbarga University, Gulbarga from 21st September 2007.
Participated One Day Seminar on Genomics and Biotherapy at
Vishweshwariah College of Applied Sciences, Gulbarga on 19th April 2007.
Attended the workshop on Intellectual Property Rights organized by the
Department of Law, Gulbarga University, Gulbarga and Centre for
Intellectual Property Rights, Research and Advocacy (CIPRA), National
Law School of India University (NLSIU), Bangalore held at Gulbarga
University, Gulbarga on February 28th – 1st March 2005.
TRAINING PROGRAMMES ATTENDED:
Successfully completed one month Crash course on Bioinformatics
conducted by Gulbarga University Gulbarga on March 1st - 31st, 2009.
Successfully completed Diploma in Advanced English conducted by Oxford
English Academy, Gulbarga on 10th June - 10th August 2009.
Successfully completed the course in Techniques in Molecular Biology
organized by Central Food Technological Research Institute held at CFTRI,
Mysore from 5th – 16th June 2006.
Participated in one day training programme on “In Vitro Fertilization”
organized by Centre for Infertility Management, Hyderabad held at MHRI
Centre for Infertility Management, Hyderabad on 1st October 2004.
MY CREDENTIALS:
Secured Second Prize in Mime held at Jnana Ganga Campus, Gulbarga
University, Gulbarga during Inter Collegiate Youth Festival from
26th – 28th November, 2004.
HIGHLIGHTS OF PRESENT RESEARCH WORK:
(Ph. D.): Research work done at Gulbarga University
Collection of samples from unique, extreme and harsh ecosystems.
Pre treatment and enrichment of samples.
Isolation of Streptomyces from the pretreated samples.
Cultural characteristic of streptomyces.
Physiological and biochemical characterization of Streptomyces on different
media.
Screening of streptomyces for bioactive molecules (Tyrosinase).
Media formulation and process standardization for production of specific
bioactive molecules (Tyrosinase).
Effect of physicochemical parameters on tyrosinase production.
Isolation and purification of tyrosinase.
Stability and kinetic studies of tyrosinase.
Preliminary studies on the, possible molecular aspects of tyrosinase.
Purified analytical tyrosinase is used for the fabrication of tyrosinase
biosensor to detect the phenolic compounds.
16s rDNA sequencing of potential isolates of Streptomyces.
Sequence analysis using Bioinformatics tools.
Blast, Fasta for the analyzed sequences to characterize up to their species
level.
(Ph. D.): Research work done at University of Helsinki
Collection of compost samples from various facilities
Isolation and screening of isolates for the production of bioactive molecules
Characterization of the potential isolates by 16SrDNA sequencing
(Ph. D.): Research work done at University of Helsinki on Pyrosequencing
Isolation of direct DNA from different compost samples
Polymerase chain reaction of the DNA by actinobacterial primers
Sequencing of the product after PCR for metagenomics studies
Analysis of metagenomics sequences to evaluate the diversity of the
actinobacteria in compost
Future approaches:
Analysis of the metagenomic sequences from Finland
TECHNIQUES KNOWN
Microbial techniques
Isolation and maintenance of microorganisms – Bacteria, Fungi,
Actinomycetes and bacteriophages.
Microscopic studies by light microscope.
Antimicrobial susceptibility test by Kirby-Bauer’s disk method and well
method.
Antibiogram
Production of Alcohol, Wine, Yoghurt, Mushrooms etc.
Assessment of quality of water (DO, BOD, COD, MPN) and milk (MBRT,
SPC, DMC).
Production of biofertilizers (Rhizobium, Azotobacter, Azospirillum, VAM, etc.)
Molecular Biology:
PCR and DNA Sequencing
Sequence Analysis
Isolation of DNA from plant and microbial source.
Isolation of plasmid DNA, Plasmid curing
Isolation of drug resistant bacteria
Agarose Gel Electrophoresis
SDS-PAGE
Restriction digestion
Metagenomics of actinobacteria
Biochemistry and Enzymology
Qualitative analysis of biomolecules (Carbohydrates, proteins, lipids,
nucleic acids etc.)
Isolation and optimization parameters studies of enzymes
(Amylase,Protease, Cellulase, Pectinase, Asparaginase).
Paper, thin layer chromatography.
Purification of Enzyme.
Fatty acid analyses of Actinobacteria
Bioinformatics and software’s:
MS-DOS, MS-Office, C++ language, Internet Knowledge
Accessing information from biological information providers (EMBL, NCBI,
PIR, PDB, GenBank Etc.)
Blastn, Clustalw, Clustalx, Fasta, Rasmol, Prime 3, Chroma, Phylip,
tree viewX, Oligo.
Instruments handled:
Camera Microscope(Nikon 32)
pH meter, BOD incubator
Electrophoresis
UV-VIS spectrophotometer
Ultra Cold centrifuge, Desktop centrifuge.
Agarose Gel Electrophoretic unit, SDS-PAGE unit, Restriction digestion kit.
PERSONAL STRENGTHS:
Independently capable of handling relevant Bioinformatics softwares, a
quick and efficient learner having a strong belief and confidence. I am also
capable of taking fast independent decision; I can also work in a team and
always an open minded researcher.
MY INTEREST:
Actinomycetes and their Bioactive molecules.
Interested in advanced techniques in Molecular and Biochemical systematic
studies such as 16s rDNA, Gene sequencing, Chemo systematic analysis of
actinobacteria and tyrosinase Enzyme engineering studies.
BIOGRAPHICAL DETAILS:
Father's Name : Shashigangadhar S.
Date of Birth : 10th October 1984
Sex : Male
Languages Known : English, Hindi, Kannada and Telugu
Corresponding Address : Department of Microbiology,
Gulbarga University,
Gulbarga – 585 106
Nationality : Indian
Religion : Hindu
Marital Status : Married
REFERENCE
Dr. Dayanand Agsar Dr. Merja Kontro
Professor Associate Professor
Department of Microbiology, Department of Environmental
Gulbarga University, Sciences
Gulbarga - 585 106 Helsinki University,
Karnataka - India. Niemenkatu 73, Lahti
Ph. No. 0091-8472-247180 (O) Finland- 14150
Mobile No.: +91-944******* Mobile No.: +358-*********
E-mail: *************@*****.*** E-mail: *****.******@********.**
Declaration:
I hereby declare that the information furnished here is correct and true to
the best of my knowledge.
Place: Gulbarga, India
Date: 18.02.2014 (Shivaveerakumar S.)
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