Dr. Nader Wahba Abdelrahman Ahmed
Gender: Male
Date of birth: 19/01/1975
Address: Home: 456 Horeya avenue, Rushdy Alexandria, Egypt
Work: Al- Horeya avenue, Bab shark, Alexandria
University, Students Hospital, Alexandria, Egypt.
Telephone: 010******** - 009***********
Email: **************@*****.***, **************@*****.***,
P ERSONAL PROFILE
I am a clinical microbiologist with more than 12 years of experience in medical
diagnostic laboratories as well as an academic staff member in the Faculty of medicine
within the microbiology department, Jazan University. Besides my experience as a
microbiologist, I have special interest in management, leadership and quality
improvement as I worked for 4 years as a laboratory director for one of the largest
laboratories in the Middle East (Al Borg Laboratories), working towards achieving
annual financial target besides my role as quality team member. I am also an
experienced researcher, as I spent more than 6 years during my MSc and PhD
undergoing research on various infectious agents and I am particularly interested in
vaccine development. I was involved in supervising post graduate and undergraduate
students in their microbiology projects in Nottingham University.
Currently I am assistant professor in the microbiology department Faculty of
Medicine, Jazan University KSA and immunology course coordinator for students in 5 th
and 6th levels, School of Applied medical Sciences Jazan University KSA. I am an active
team member of CPD and Quality management departments, Faculty of medicine,
Jazan University, KSA as well as coordinator and general secretary for Jazan University
hospital committee for construction and establishment.
E DUCATION AND QUALIF ICATIONS
2011- PhD in Microbiology and Immunology
University of Nottingham, UK
2006- MSc in Medical Microbiology and Immunology
Alexandria University, Egypt
2005 -Diploma in Total quality Management in Health care
American University in Cairo, Egypt
1992-1998 - MB ChB. Bachelor’s of Medicine and General Surgery
Alexandria University, Egypt
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W ORK EXPERIENCE
September 2012-present: Assistant professor of Microbiology and Immunology, Faculty
of Medicine, Jazan University, Saudi Arabia
Main Tasks:
- Lecturing microbiology and Immunology to medical Students, course contents are
following a module system.
- Course coordinator for basic and advanced immunology courses for undergraduate
students in the college of Applied biomedical sciences.
- Supervising a selected patch of students in their graduation projects and in the
dissertation writing. Involved in the department research meetings and activities.
- Clinical duty in King Fahd general hospital in Jazan region within the microbiology,
immunology and the molecular diagnostic departments
- Active member in continuous development department, faculty of medicine.
- Active member of quality accreditation and improvement department, faculty of
medicine, working towards NCAAA accreditation.
- Coordinator and general secretary for Jazan University hospital committee for
construction and establishment.
o Responsible for meetings organisation and reporting to the head of the
committee and to the dean of the Faculty of Medicine.
o Establishing connection with the committee members and internal and
external consultants giving advice on the University hospital construction.
o Head of laboratory advisory committee for the University hospital construction.
April 2000-present: Clinical Microbiology Consultant, Alexandria University, Students
Hospital, Alexandria, Egypt. (Permanent job and on leave now as Ass. Prof in Jazan)
Main Tasks:
Working as a part of a team that handles all the specimens for the clinical microbiology
laboratory of the hospital. This included the different routine cultures as well as blood and
CSF cultures, performing ward rounds, viral Ag and Ab detection, Widal test as well as
mycological sample examination. Team member in hospital infection and control, and head of
quality and hospital accreditation department.
June 2010- August 2011: Clinical Microbiology specialist, Derby Royal Hospital, Derby,
UK.
Main Tasks:
I had experienced bench work where appropriate, and carried out clinical duties
which include phoning out results, taking calls from clinical colleagues concerning
interpretation of results and giving advice regarding diagnosis and management of
patients. Also, as a part of this post I had to deal with infection control queries.
I was involved in Senior Management meeting each week; which gave me experience
into laboratory management in a District General Hospital.
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Nov 2006- Apr 2011: Full time PhD student, Molecular Bacteriology and Immunology
Group, Microbiology, School of Molecular Medical Sciences, University of Nottingham.
Skills acquired:
Molecular techniques for gene mutagenesis, gene cloning, protein expression and
purification. As well as various methods for investigating protein-protein interactions,
cell signalling tissue culture, in addition to the more classic techniques of handling the
different bacteria, whether Gram positive (such as Staphylococci and Streptococci) or
Gram negative (Neisseria, Haemophilus, E.coli, Pseudomonus).
Sep 2002- Jan 2006: Director of Laboratory, Al Borg Medical Laboratory, Alexandria,
Egypt. (In conjunction with University of Alexandria job)
Main Tasks:
Management responsibilities including budget control, personnel management, staff
appraisal and training, team working, negotiation skills, strategic planning,
preparation of a business plan.
Consultations to physicians about the laboratory diagnostic investigations required for
medical disorders and interpretation of the results in all specialties.
Workload measurement and financial management of the resources and the expenses.
Laboratory safety and the transportation of medical samples.
Assuring that laboratory procedures following legislation and regulation governing
laboratories.
Regular audits to check reagents, calibrators, instrument maintenance and technical
competence (as Alborg lab is joining three international quality assurance programs
namely, Quality assurance program of the college of American pathologists (CAP) for
Excellence in laboratory testing (EXCEL) (USA), RIQAS International Quality
Assessment Scheme and Certificate of the international standardization of
Organization (ISO 9001-2008).
Nov 1998- Sep 2002: Medical marketing team member, Al Borg Medical Laboratory,
Alexandria, Egypt. (In conjunction with University of Alexandria job)
Main Tasks:
Develop and build work group, and communicates with clients and colleagues while at
work.
Promote service to clients by visits and organizing group presentations and
conferences.
Signing contacts with companies to provide them with medical service.
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Apr 2000- Jun 2001: General practitioner, Egyptian armed forces Hospitals, Egypt.
Main Tasks:
Receiving out patients, asking for investigations, evaluating their results and managing
them accordingly.
Mar 1999- Feb 2000: House Officer, Alexandria Main University hospital, Alexandria
Egypt.
Main Tasks:
In the wards: following up admitted patients, ordering investigations and taking
patient samples, swabs, bone marrow specimens, pleural and abdominal aspiration.
Evaluating preoperative patients and doing the necessary tests, monitoring post
operative patients, and those in the coronary care and the intensive care unit.
Following up the investigation results, managing accordingly, and consulting senior
doctors if necessary,
In the Emergency department: Initial evaluation of patients presenting to the
emergency departments of Paediatrics, Surgical, Obstetrics and Gynaecology, and
General Medicine Hospitals, giving the necessary resuscitation and management
measures required accordingly before referring to the Senior House Officer on call.
Educational: Preparing case reports and presenting them in weekly presentations.
A DDITIONAL INFORMATI ON:
Fluent in Arabic and English languages (IELTS score of 7.5 in all sections).
Skilled in using Windows and Mac computer operating systems, and their application
tools.
Using analytical or scientific software through my career, list of familiar software
o Basic Local Alignment Search Tool BLAST.
o DNA sequence analysis software.
o Gene Finder.
o Image capture and analysis software.
o Protein databases.
o Statistical software-SPSS.
o iLAB – software used for reporting in Pathology department in Derby Royal
Hospital.
o AlBorg laboratory software (lab operatoinal module, owned and copywirted by
Al Borg Laboratory).
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P UBLICATIONS:
Ala'aldeen, D. A., Oldfield, N. J., Bidmos, F. A., Abouseada, N. M., Ahmed, N. W., Turner, D. P.,
Neal, K. R. & Bayliss, C. D. Carriage of meningococci by university students, United kingdom.
Emerg Infect Dis 17, 1762-1763.
Hashish M H, El-Barrawy MA, Mahmoud OA, Abdel Rahman NW. TT virus among blood donors
in Alexandria. J. Egyp Pub Heal Ass 2005; 80: 651-64.
T HESIS SUPERVISION
During my research in the University of Nottingham (2006-2011) was responsible for two
MSc students as a technical supervisor for their laboratory experiments.
Currently supervising nine undergraduate students during their research projects
C ONFERENCES AND MEET INGS
Harrogate International Centre meeting, 30 March - 2 April 2009, Society for general
Microbiology.
16th International Pathogenic Neisseria Conference, Rotterdam, 7-12 September 2008.
Spring meeting 31 March- 3 April 2008 Edinburgh international conference centre, Society
for general Microbiology.
17th International Pathogenic Neisseria Conference 2010, Banff, Calgary, 11-16 September
2010.
S HORT COURSES
Lecturing for Learning.
Introduction to image and photo editing.
How to prepare an effective poster presentation.
MS Excel, MS Powerpoint, Exploiting the power of MS Word.
Building a bibliography.
Demonstrating in laboratory practicals.
Post-genomics and bioinformatics.
Referencing and citing using Endnote.
Getting started with research design and statistics.
Qualitative research and quantitative research.
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SPSS for researchers.
Computing and molecular biology.
Critical analysis of scientific literature.
Mathematics in the lab.
A practical look at core teaching skills.
Supporting students who are doing undergraduate projects and dissertations.
AL Borg Laboratories training courses (2002-2006).
L ABORATORY TECHNOLOG Y
Machines and systems trained and used
Cobas Amplicor, Roch Diagnostics.
Fluorescent techniques and microscopy.
Applied Biosystems step one and step one plus Real time PCR.
Roch diagnositics autoanalysers cobas c311 and e411.
VITEK automated microbiology system.
Biacore (Surface Plasmon Resonance Instrument), for research binding assays.
iQ®200 automated flow cytometry analyser.
S1000 Thermal Cycler, BIO-RAD.
ELISA reader (Biotek EL800).
Cell culture systems.
NanoDrop 1000 Spectrophotometer.
2-D and 3-D electrophoresis for Proteomics.
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P HD PROJECT
TspA interacting proteins of Neisseria meningitidis
Nader Ahmed, Jafar Mahdavi, Neil Oldfield, Karl Wooldridge & Dlawer Ala’Aldeen
University of Nottingham, United Kingdom
Objective: Neisseria meningitidis is a commensal bacterium of the human nasopharynx.
Occasionally, it gains access to the bloodstream and causes septicaemia and subsequently
meningitis. T-cell stimulating protein A (TspA) is an immunogenic, conserved, T-cell and B-
cell stimulating protein of N. meningitidis that is required for optimal adhesion to human cells.
The role of TspA in the adhesion process is thought to be indirect since it is predicted that the
N-terminus is localised to the periplasmic space with an inner membrane-spanning domain
linking it to a cytoplasmic C-terminal domain); the aim of this study was to identify proteins
within the meningococcal envelope that interact with TspA.
Methods: Four overlapping recombinant fragments of TspA were expressed and purified as
fusion proteins with the pGEX-2T-encoded glutathione-S-transferase protein. A receptor
activity-directed affinity tagging protocol was employed to identify TspA-interacting
meningococcal proteins. Interactions between TspA and candidate proteins were investigated
further using ELISA and surface plasmon resonance.
Results: Four putative TspA-interacting proteins were identified: PilQ and PilT (components
of the type IV pilus machinery); the major outer membrane protein, PorA, and the protein
chaperone, ClpB. Furthermore, the portion of TspA responsible for interaction with PorA was
confirmed to be the N-terminus.
Conclusions: Periplasmic domains of TspA interact with several outer membrane proteins
which have a key role in meningococcal pathogenesis and adhesion to host cells.
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R EFEREES:
Name of Referee 1: Dlawer Ala'Aldeen
Title and awards: Professor of Microbiology, MBChB, DTM&H, MSc, PhD, FRCPath
Address: Molecular Bacteriology and Immunology Group, Division of Microbiology &
Infectious Diseases, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH,
United Kingdom
Email: *******@******.**********.**.**
Name of Referee 2: Farah Yazdani
Title and awards: Consultant Microbiologist & Head of Service for Microbiology
Address: Derby Royal Hospital, Uttoxeter Road, Derby, DE22 3NE, United Kingdom
Email: *****.*******@**************.***.**
Name of Referee 3: Mohamed Abbas El-Barrawy
Title and awards:, Professor of Microbiology, MBChB, MSc, MD
Address: High institute of public health Alexandria University 165, Horreya Avenue, Hadara
Alexandria, Egypt.
Email: drelbarrawy @ hotmail.com
Name of Referee 4: Mona Hassan Nashaat Hashish
Title and awards: Assistant Professor of Microbiology, MBChB, MSc, MD
Address: High institute of public health Alexandria University 165, Horreya Avenue, Hadara
Alexandria, Egypt.
Email: ********@*****.***
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