Project Human

Mikir Patel

Drexel University College of Medicine

*********@*****.***

Summary

I am a biomedical science graduate majoring in Molecular and Cellular Biology and Genetics actively looking

for full-time positions to impart my skills and develop expertise in fulfillment of personal and organizational

development.

Expertise and accomplishments include:

• Strong working experience in conducting experiments in molecular and cellular biology, biochemistry, and

cytogenetics.

• Develop research strategies in molecular and cancer biology.

• Analytical and problem solving skills in interpreting a project plan, formulating hypotheses, and testing

data-driven experimental models.

• Strong organizational skills with ability to manage multiple tasks in a timely manner.

• Ability to perform in a goal-oriented environment and coordinate team efforts.

• Excellent written and verbal communication skills.

• Highly entrepreneurial and cooperative.

• Conducted a thesis project studying the homology search activity of the human DNA repair protein, RAD52,

in the homologous recombination pathway.

• Conducted a primary screening of 70 compounds as RAD52 inhibitors and identified 35 hits.

Experience

Research Associate at Drexel University College of Medicine

January 2012 - Present (2 years)

• Directed a thesis project on "Homology Search Activity of Human RAD52".

• Exhibited excellent working skills ranging from developing comprehensive project plans, formulating

hypotheses, result-oriented experimentation, data interpretation and analysis.

• Acquired expertise in DNA synthesis and purification, gene cloning, bacterial transformation, in vitro

protein-DNA interaction assays, radioligand binding assays.

• Conceived original concepts and accomplished novel findings.

• Conducted a primary screening of 70 compounds as human RAD52 inhibitors and identified 35 hits.

• Participated in several nationwide cancer biology and molecular biology symposiums and seminars.

Research Associate at Drexel University College of Medicine

September 2011 - December 2011 (4 months)

• Worked on a project studying the dose-dependent effect of ionizing radiation (X-rays) on the DNA damage

pathway in non-tumorigenic human epithelial cell line, MCF-10A.

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• Mastered conventional molecular biology techniques including polyacrylamide and agarose gel

electrophoresis, western blotting, tissue culture (handling cell lines - MCF10A, MCF7, 393T), DNA isolation

and purification, and cell transfection.

Research Intern at Institute of Human Genetics

January 2011 - June 2011 (6 months)

• Carried out a research project on "Cytogenetic Study of Digeorge Syndrome".

• Established skillfulness in routine cytogenetic diagnostic techniques which included patient sample

collection, sample tissue culture set up, culture harvest, slide preparation, Giemsa-banding, Karyotyping and

FISH (Fluorescence in situ hybridization) analysis.

• Demonstrated proficiency in maintaining accurate patient records and create genetic pedigrees.

Project Trainee at Sterling Biotech Limited

January 2011 - January 2011 (1 month)

• Carried out a training project on manufacturing and processing of Coenzyme Q10.

Projects

Investigation of the homology search activity of human RAD52

January 2012 to Present

Members:Mikir Patel

Exogenous and/or endogenous agents such as ionizing radiation, anti-cancer drugs and reactive oxygen

species can result in DNA double strand breaks (DSB), a major form of DNA lesion. Inability to repair such

DNA lesions can result in loss of genomic integrity eventually causing death. My project focused on

investigating the mechanism of the homology search activity of human DNA repair protein, RAD52, during

the process of homologous recombination, a key mechanism to repair DSBs. Significant findings of the

project include - the requirement for low level of single stranded DNA substrate homology by human

RAD52, the strong preference of human RAD52 for highly supercoiled DNA and binding characterization of

the protein to DNA. My project also included screening of 70 compounds as RAD52 inhibitors in the DNA

binding assay of which 35 hits were identified.

Certifications

Radiation Protection

Radiation Physics and Safety Office November 2012

Courses

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Master of Science (MS), Molecular and Cellular

Biology and Genetics

Drexel University College of Medicine

Molecular Structure and Metabolism

Special Topics in Molecular and Cellular Biology and

Genetics

Cells to Systems

Biostatistics

Advanced Cell Biology

Experimental Approaches to Biochemical Problems

Responsible Conduct of Research

Macromolecular Structure and Function

Bachelor of Technology (B.Tech.), Biotechnology

Dr. D.Y.Patil University

Cell Biology and Developmental Biology

Biochemistry

Molecular Biology

Animal and Plant Cell Culture

Genetic Engineering

Molecular Cell Signaling

Microbial Biotechnology

Enzymology

Microbiology and Immunology

Languages

English (Full professional proficiency)

Gujarati (Native or bilingual proficiency)

Hindi (Native or bilingual proficiency)

Skills & Expertise

Microsoft Office

Cell Culture

Western Blotting

Gel Electrophoresis

Plasmid Isolation

PCR

DNA extraction

Radioactive Materials

DNA-Protein Interaction

GraphPad Prism

Southern Blot

RNA isolation

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PCR primer design

Patient Education

Industrial Manufacturing

Bacterial Cell Culture

Bacterial Transformation

Gel Filtration Chromatography

Molecular Biology

ELISA

Assay Development

In Vitro

Cell

Purification

Education

Drexel University College of Medicine

Master of Science (MS), Molecular and Cellular Biology and Genetics, 2011 - 2013

Dr. D.Y.Patil University

Bachelor of Technology (B.Tech.), Biotechnology, 2007 - 2011

Interests

Reading management and leadership books, Exploring cutting-edge technologies, Networking

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Mikir Patel

Drexel University College of Medicine

*********@*****.***

Contact Mikir on LinkedIn

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Contact this candidate