Technician Management

Pramod Phuyal

** ******* ****

******.******@*****.***

Watertown MA, 024**-***-***-

0217

Objective To obtain Manufacturing Technician position using skills learned

through biomedical training

Lab Experience

Buffer Preparation (by molarity and percent)

Cell Culture Methods-TniP3 cell lines,prepared media using sterile

technique,hemocytometer use

Protein Quantitation-Lowry and Bradford Methods

Column Chromatography-used gel filtration to purify gels and proteins

Molecular Biology Techniques-PCR,Western Blots

ELISA-both direct and indirect methods

Restriction Enzyme digests of DNA followed by gel electrophoresis

separation of fragments

Bacterial Transformation-transformed E.coli with ampicillin-resistant

plasmids and evaluated growth

Knowledge of cGMPs, SOPs, GLP and proper documentation

Education

Cambridge Biomedical Careers Program Boston, MA Sep

2012 - May 2013

Bunker Hill Community College / Just-A-Start Corporation

Certificate in Biomedical Sciences

An intensive nine month academic program with over 300 hours of training in

biomedical techniques

Courses: Fundamentals of Chemistry, Fundamentals of Biology, Medical

Terminology,

Introduction of Biotechnology, Introduction to Computers

Mass Bay Community College Wellesley, MA Sep 2010 -

Dec 2011

Associates Degree Progress

Courses: Computer Science, Macro Economics, Communications, Statistics,

Critical Thinking

Employment History

AstraZeneca Pharmaceutical Company Waltham,MA

Jun 2013 - Present

Lab Support Technician

Media Preparation, Solvent preparation,solution preparation and Buffer

preparation in various department.

Enter data in the Laboratory Information Management System (LIMS).

Assist in the assembly and disassembly of process equipment.

Carry out cGMP manufacturing operation utilizing Standard Operating

Procedures(SOP),with Good Laboratory Practice (cGLP), Batch Records and

forms preparation,perform with sterlize technique.

Record data in to log book, Operates production equipment according to SOPs

in their assigned areas.

Carry out work in a safe manner, notifying management of safety issue and

risk.

Adhere to AstraZeneca departmental safety policies, SOPs and guidelines

while performing competency in facility safety protocols.

Perform schedule cleaning of equipment with supervision of scientist.

Perform Supply Ordering and receiving for Media Preparation Department.

Walgreens Company Brookline, MA

Jun 2005 - Present

Photo Technician

Adhere to photo printing protocols to ensure proper resolution

Maintain photo laboratory, chemicals and stocks

Change chemicals as needed following all health and safety protocols,

Store Team Leader Feb 2010 -

Aug 2010

Promoted to manage a team of 15 employees

Executed cash audits and cash out procedures

Maintained a clean and presentable store

Languages

Fluent in English, Nepali and Hindi

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