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Project Training

Location:
Duarte, CA
Posted:
October 29, 2013

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Resume:

Sukhada Bhojane

Research Intern at City of Hope, Beckman Research Institute

*********@*****.***

Summary

Highly impelled researcher with expertise in molecular and stem cell biology and cell culturing techniques;

nanoparticle synthesis, characterization and functionalization techniques in addition to good laboratory

practices. Adept at working independently as also in a team.

Experience

Research Fellow at City of Hope

October 2012 - September 2013 (1 year)

1.) Project title: “Magnetically Induced Movement of Cells for Targeted and Effective Immunotherapeutic”

•Carried out the synthesis and characterization of CpG coated iron oxide nanoparticles (IONP) using

Dynamic Light Scattering (DLS) and Zeta Potential (ZP).

•Evaluated the uptake of IONP conjugates by enhanced Green Fluorescent Protein (eGFP) expressing

macrophage (BV2) cells.

•Determined the movement and alignment of cells in the presence of external magnetic field using various

in-vitro cell based assays.

1.) Project Title: “Stem Cell-Mediated Targeting of Therapeutic Nanoparticles to the Tumor Site”

•Was responsible for synthesizing and characterizing of anti-cancer Drug loaded PLGA nanoparticles

•Evaluated the activity of the drug i.e. initial loading and percent retention by UV-Vis, Fluorescence and

HPLC.

•Conducted various in vitro cell based assays to substantiate the tumor tropic movement of NSCs

•Currently working on different linkage strategies in order to accomplish NSC-drug loaded nanoparticle

conjugates.

Research Trainee at University of Southern California

July 2012 - July 2012 (1 month)

Attended a comprehensive hands-on training program pertaining embryonic stem cell biology, culturing and

techniques in conjunction with maintenance of pluripotent stem cells.

Graduate Student Researcher at California State University, Channel Islands

February 2012 - March 2012 (2 months)

Optimized the growth conditions of mouse mesenchymal stem Cells (mMSCs) in 3D

fibrin scaffold constructed of varying concentrations of fibrinogen and thrombin

• Employed live/dead assay and fluorescence microscopy to check the cell

proliferation rate of mMSCs

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Student Researcher at Advanced Center for Training, Research and Education in Cancer (ACTREC)

March 2010 - March 2010 (1 month)

•Attended a comprehensive training program at CRI (Cancer Research Institute) about various different

aspects of investigations in cancer biology addressing the basic and translational research queries using cells

and human tumors in cultures.

•Learned about the basic techniques and equipments that are used routinely in cancer biology research.

Honors and Awards

Research Intern

California Institute of Regenerative Medicine

September 2012

Amgen Industry-Sponsored Scholarship

Amgen

January 2013

Languages

English (Full professional proficiency)

Hindi (Native or bilingual proficiency)

Marathi (Native or bilingual proficiency)

Courses

Master of Science (MSc), Biotechnology

University of Mumbai

Cell Biology

Immunology

Genetics

Molecular Biology

Biochemistry

Master of Science (MS), Biotechnology

California State University-Channel Islands

Molecular Biology

Genomics and Proteomics

Tissue Engineering

Bioinformatics

Advanced Techniques in Stem Cell Technology

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Skills & Expertise

Molecular Cloning

Stem Cell Research

Bioinformatics

Cancer Cell Biology

Mammalian Cell Culture

recombinant DNA technology

Biotechnology

Pharmacology

Cancer Stem Cells

Nanobiotechnology

Nanoparticles

Polymer Science

Polymer Characterization

Molecular & Cellular Biology

Pharmacokinetics

Molecular Pharmacology

DNA extraction

DNA sequencing

RNAi

Affinity Chromatography

Site-directed Mutagenesis

Immunohistochemistry

Immunocytochemistry

HPLC

Protein Purification

Nanodrop

qPCR

Flow Cytometry

molecular

Western Blotting

Gel Electrophoresis

Strategic Planning

Transfection

Confocal Microscopy

Lyophilization

Zeta Potential

DLS

Hyperspectral Imaging

Brightfield

Mouse Handling

Education

California State University-Channel Islands

Master of Science (MS), Biotechnology, 2011 - 2013

University of Mumbai

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Master of Science (MSc), Biotechnology, 2008 - 2010

Interests

Reading, Hiking, New technologies, Photography

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Sukhada Bhojane

Research Intern at City of Hope, Beckman Research Institute

*********@*****.***

Contact Sukhada on LinkedIn

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Contact this candidate