Management Project

Sean Adhikari

**** * ******* **., *** **** Cell: 512-***-****

Houston, TX 77063 *********@*****.***

PROFESSIONAL PROFILE

Motivated research professional with hands on experience in the areas of

biochemistry and molecular biology. Strong analytical skills with

demonstrated ability to conduct experiments, analyze data, and provide

insight; committed to quality process and outcomes. Highly skilled in

performing and optimizing laboratory procedures using advanced equipment

and effectively works independently and in teams. Excellent ability to

communicate with management and co-workers and plan projects effectively.

TECHNICAL SKILLS

Molecular Biology Skills: FPLC, SDS-PAGE, ELISA (immunoassays),

Western/Northern blotting, PCR, Electrophoresis, DNA/RNA/Protein

purification, DNA site-directed mutagenesis, RNA synthesis/purification, RT-

PCR, UV/VIS, cell culture and suspension, plus others

Biochemistry Skills: Solution and Reagent preparation, Enzymatic assays,

Radioisotope tracer assays, plus others

Computer Software: MS Word/Excel/Powerpoint (Mac & Windows), Kaleidograph

(Mac), SPSS (Windows), GraphPad Prism (Mac & Windows), STATISTICA (Mac)

Animal Skills: Mouse handling and management, peritonial injections,

blood/plasma collection, ear tagging, tail clipping

BACKGROUND SUMMARY

Project Management Troubleshooting Logic/Scientific

Thinking

Research/Data Analysis Research Publications Inventory/Ordering

Assay Development Scientific Literature Seminars/Presentations

Search

Database Management Protocol Optimization Explanations/Conclusions

PROFESSIONAL ACCOMPLISHMENTS

Baylor College Of Medicine, Houston, TX 2013 - Present

Research Technician II

Behavioral and biochemical studies of neurological disorders in mice.

Skills used: PCR genotyping, electrophoresis, tissue/organ harvesting,

animal husbandry

Xbiotech USA, Inc, Austin, TX 2010 - 2012

Research Associate

Optimized protein purification procedures and assisted in production and

analysis of cytokines and antibodies (immunoassays) used to treat various

diseases in clinical trials. Provided R&D with purified proteins for

various assays.

Skills used:

ELISA: Successfully performed assay within 10% CV, revised SOP for QC under

GLP/GMP guidelines

FPLC: Provided routine buffer formulations for protein analysis, antibody

and cytokine purification, optimization of AKTA buffer gradient and

fraction collection

Cell culture/transfection: passaging non-adherent mammalian cells, PEI

transfection

Other skills: Western blotting, Giga DNA plasmid preps

Baylor College of Medicine, Houston, TX 2008 - 2010

Research Technician III

Contributed to ion-channel study and aptamer project. Successfully created

mutant lines of mRNA, with single base-pair substitutions, of ion-channels

and optimized PCR for aptamer project.

Skills used: site-directed mutagenesis, PCR, PAGE/agarose electrophoresis,

DNA/RNA purification

University of Texas Health Science Center at San Antonio, San 2006 -

Antonio, TX 2008

Research Associate

Created a serological profile of STD patients for reactivity to Mycoplasma

Genitalium. Tested patient antibodies for reactivity to Mycoplasma

proteins using Western blotting.

Skills used: Bacterial transformation, Protein expression/purification, SDS-

PAGE, Western blotting

Texas A&M University, College 2004 - 2006

Station, TX

Graduate Research Assistant

Gained insight in relationship between fatty acid and glucose metabolism in

mice. Performed in vitro mouse muscle incubations and measured fatty acid

and glucose oxidation. Result: Found different patterns in metabolism

after feeding standard versus high-fat diets.

Contributed to scientific literature and presented work in seminar.

Established protocols for procedures, published results and conclusions,

and presented work in departmental seminar.

Optimized glucose measurement protocol. Searched literature on NCBI PubMed

website and adjusted parameters to our needs. Arranged setup for muscle

incubation and performed experiments using setup.

EDUCATION

Master of Science in Genetics, Texas A&M University, College 2006

Station, TX

Bachelor of Science in Cell & Molecular Biology, University of 2003

Washington, Seattle, WA

PUBLICATIONS

Adhikari S, Erol E, and Binas B. Increased glucose oxidation in H-FABP

null soleus muscle is associated with defective triacylglycerol

accumulation and mobilization, but not with the defect of exogenous fatty

acid oxidation. Mol Cell Biochem, 2007 Feb; 296(1-2):59-67.

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