Development Medical

John C. Gill, Ph.D.

Boston, MA 857-***-****

*********@***.***

Ph.D. Scientist with eight years start-up biotechnology experience and

expertise in process development, neuroscience and epigenetics. Designs and

implements novel research strategies to understand human disease at genetic

and physiological levels. Collaborates successfully across research and

clinical platforms to establish therapeutic tools and innovative

treatments. Interested in novel technology development and identifying

promising frontiers in biotechnology.

SPECIALTIES:

. Cell signaling and physiology

. Neuroendocrinology

. Translational research

. Epigenetic biochemistry

. Gene expression

. Systems / Network biology

. Preclinical development

. Cell line development

. Clinical testing guidelines

EDUCATION:

. Ph.D., Neuroscience and Integrated Physiology, University of Colorado,

Boulder

. B.A., Molecular, Cellular and Developmental Biology, University of

Colorado

ACADEMIC & INDUSTRY EXPERIENCE:

Harvard Medical School, Boston, MA Faculty / Instructor in Medicine

2009-2013

Brigham & Women's Hospital, Division of Endocrinology Associate

Biochemist

A translational laboratory that identifies novel neuroendocrine regulators

and the basis of reproductive disorders by examining human genetics and

physiology in cell lines and rodent models. Mentored by Drs. Ursula

Kaiser, MD, BWH, William F. Crowley, MD, Massachusetts General Hospital and

Joseph Majzoub, MD, Boston Children's Hospital.

. Discovered regulatory role of histone demethylases in the epigenetic

control of pubertal timing

. Developed physiological estrogen-sensitivity assays by neuropeptide

expression analysis

. Expertise in GPCR and nuclear receptor signaling in the regulation of

neuronal differentiation

. Trained to support proof of concept clinical trials and early development

of novel therapeutics

. Research program management, including budget, funding and research team

. Provided leadership, training and mentoring for post-doctoral fellows and

research assistants

. Faculty at Harvard Medical School, taught molecular biology and

physiology classes

Harvard Medical School, BWH Biomedical Research Inst. Postdoctoral

Fellow 2006-2009

. NIH Awarded Investigator in the neural regulation of reproduction and

metabolism

. Developed novel research strategies and models, e.g. viral-mediated gene

therapy and RNA interference (shRNA) use in the brains of mutant and

transgenic mice

. Investigated specific disorders of human reproduction in animal models

and cell culture

University of Colorado, Boulder, CO Predoctoral Fellow, Ph.D.

Student 2000-2006

Dept. of Integrative Physiology- Developmental and comparative

neuroendocrinology research, lab of Dr. Pei-San Tsai

. NIH Awarded student in a comparative lab, identified the developmental

and signaling roles of FGF receptor tyrosine kinases in the

differentiation of hypothalamic GnRH-producing neurons

Vitro Diagnostics Inc., Littleton, CO Senior Scientist

1994-1999

A start-up diagnostic and stem-cell focused biotechnology company-

manufactures and sells proteins and cell lines

. Performed cell line development and generated immortalization technology

for human cells

. Led discovery and development of cancer antigens for diagnostic markets

INDUSTRY EXPERIENCE (cont.):

Vitro Diagnostics Inc., Littleton, CO Senior Scientist

1994-1999

. Introduced cell culture technology and initiated recombinant human

protein production (cGLP)

. Generated company's first recombinant products and intellectual property

to be awarded SBIR

Synergen Inc., Boulder, CO Senior Research Associate

1991-1993

A start-up biotech company that specialized in the development of

recombinant products to treat human inflammatory and neurological

disorders- Acquired by Amgen in 1994.

. Participated in fermentation and purification process development for

cGMP production of recombinant proteins, including IL-1ra (Kineret),

GDNF, TNFbp, FGF1, SLPI and BDNF

. Instrumental in laboratory (10L) and pilot scale (250L) bioreactor

facility setup and design

. Oversaw research laboratory, pilot facility and staff to develop human

pharmaceuticals bioprocessing protocols (microbial fermentation and

protein purification by chromatography)

. Promoted through positions in Manufacturing, Process Development and then

Research and Development in a fast-paced startup environment, managing

process operations in 24/7 setting

BIBLIOGRAPHY:

Gill JC, Kwong C, Nguyen D, Okay S, Breakefield X, Carroll RC, Conner D, Ng

P and Kaiser UB. Gene Therapy Rescue of Reproductive Parameters in GnRH-

Deficient (hpg) Mice Using Helper-Dependent Adenoviral Vectors. (In

Preparation)

Gill JC, Kwong C, Nguyen D, Clark E, Fang R, Pojogo L, Carroll RC, Shi YG

and Kaiser UB. The Histone Demethylase LSD1 Modifies the Epigenetic

Regulation of Pubertal Timing (In Preparation).

Abreu AP, Dauber A, Macedo D, Noel SD, Brito VN, Gill JC, Cukier P,

Thompson IR, Navarro VM, Gagliardi PC, Rodrigues Y, Kochi C, Longui CA,

Beckers D, de Zegher F, Montenegro LR, Mendonca BB, Carroll RS, Hirschhorn

JN, Latronico AC, and Kaiser UB. Central Precocious Puberty Caused by

Mutations in the Imprinted Gene MKRN3. NEJM 2013; DOI:

10.1056/NEJMoa1302160

Gill, JC, Navarro V, Kwong C, Noel S, Martin C, Xu, S, Connor D, Clifton D,

Carroll R, Steiner R, Kaiser UB. Neurokinin B (NKB) Expression as an Early

Marker of Pubertal Onset with Lower Sensitivity to Sex Steroid Negative

Feedback than Kiss1. Endocrinology 2012; 153(10):4883-93.

Gill JC, Wang O, Kakar S, Martinelli E, Carroll RS, Kaiser UB. Reproductive

Hormone-Dependent and -Independent Contributions to Pubertal Kisspeptin

Activation in GnRH Deficient Hypogonadal Mice. PLoS One 2010; 5(7):e1911.

PMID: 20689830.

Jeong K-H*, Gill JC*, Nose V, Parlow AF, Kaiser, UB. Expression of a

Gonadotropin-Releasing Hormone Receptor-Simian Virus 40 T Antigen Transgene

has Gender-Specific Effects on the Reproductive Axis. (*, co-first authors)

Endocrinology 2009; 150(7):3383-91.

Gill JC, Wadas B, Chen P, et al. The Gonadotropin-Releasing Hormone (GnRH)

Neuronal Population Is Normal in Size and Distribution in GnRH-Deficient

and GnRH Receptor-Mutant Hypogonadal Mice. Endocrinology 2008; 149(9):4596-

4604.

Gill JC, Tsai PS. Expression of a Dominant Negative FGF Receptor in

Developing GNRH1 Neurons Disrupts Axon Outgrowth and Targeting to the

Median Eminence. Biology of Reprod. 2006; 74:463-472.

BIBLIOGRAPHY (cont.):

Tsai PS, Gill JC. Mechanism of Disease: Insights into X-linked and

Autosomal Dominant Kallmann Syndrome. Nature Clinical Practice

Endocrinology & Metabolism 2006; 2(3):160-171.

Gill, JC. Developmental Regulation of Gonadotropin-Releasing Hormone

Neurons by Fibroblast Growth Factor Receptor Signaling [Dissertation

Thesis] 2005; The University of Colorado, Boulder, CO.

Gill JC, Moenter SM, Tsai PS. Developmental Regulation of Gonadotropin-

Releasing Hormone Neurons by Fibroblast Growth Factor Signaling.

Endocrinology 2004; 145(8):3830-9.

Tsai P-S, Moenter SM, Postigo HR, El Majdoubi M, Pak TR, Gill JC,

Paruthiyil S, Werner S, Weiner RI. Targeted Expression of a Dominant

Negative FGF Receptor in GnRH Neurons Reduces FGF Responsiveness and the

Size of GnRH Neuronal Population. Mol Endocrinology 2005; 19(1):2225-23.

PATENTS:

Burnett B, Gill JC, Musick JM, inventors; Vitro Diagnostics Inc., Assignee.

"Immortalized Cell Lines and Methods of Making the Same", International

Patent WO0043500, European Patent EP1144597. 2000.

AWARDS AND HONORS:

2012 The Eugenia Rosemberg Award for New Faculty and New Investigators, The

Endocrine Society.

2012 The Harvard Medical School Excellence in Tutoring Award, HMS Academy

for Teaching.

2009 Career Development Scholarship (K12), Building Interdisciplinary

Research Careers in Women's Health, National Institute of Health, Office of

Research on Women's Health, "Sexual Dimorphism of Reproductive Function:

The Role of Kisspeptin".

2009 Travel Grant Award for Excellence in Research, The Endocrine Society

90th Annual Meeting.

2009 The Endocrine Society Trainee Day Program Recipient, The Endocrine

Society 90th Annual Meeting.

2008 Women in Endocrinology Young Investigator Award, The Endocrine

Society.

2007 International Award for Excellence in Research, The Endocrine Society

National Meeting.

2005 Poster Presentation Award, Society for Neuroscience, Front Range

Chapter, Neuroscience Meeting.

2004 National Research Service Award Individual Fellowship (NRSA), National

Institute of Health, Division of Child Health and Human Development, "The

Regulation of GnRH Neuronal Development by FGF-Signaling."

2004 Quest Diagnostics Young Investigator Award, The Endocrinology Society

National Meeting.

2004 Poster Presentation Award, Society for Neuroscience, Front Range

Chapter, Neuroscience Meeting.

2003 Oral Presentation Award, Society for Neuroscience, Front Range

Chapter, Neuroscience Meeting.

2001 Beverley Sears Competitive Grant,

University of Colorado: "Role of Thyroid Hormone in Gonadotropin-Releasing

Hormone (GnRH) Neurogenesis".

2001 University of Colorado Departmental Competitive Grant, "Effects of

FGF2 on GnRH Neuron Migration".

2001 Chancellor's Teaching Fellowship, University of Colorado, Boulder.

RELEVANT CERTIFICATE TRAINING:

2011 Workshop: Role of the Manager- Partners Healthcare Education and

Training Program

2010 Intensive Training in Translational Medicine Course - Harvard Clinical

and Translational Science Center

2008 Leadership Training for Scientists - Massachusetts Biotechnology

Council

2008 Responsibility in Clinical Research Studies- Center for Clinical

Investigation, Brigham and Women's Hospital

2008 Genetics in Clinical Research - Center for Clinical Investigation,

Brigham and Women's Hospital

2007 Overview of Clinical Research - Massachusetts Biotechnology Council

2007 Introduction to Clinical Research - Center for Clinical Investigation,

Brigham and Women's Hospital

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