Plant Assistant

SRILAXMI SARIKONDA

*** *** ******* *****, ***********, NC-27560

***********@*****.**.** ? 203-***-****

OBJECTIVE:

To obtain a position in biotechnology/pharmaceutical firm to advance in

career and hone my knowledge.

EDUCATION:

North Carolina State University, Raleigh, NC.

Graduate Certificate in Molecular Biotechnology.

Nov 2008

Acharya Nagarjuna University-Andhra Pradesh, India.

Masters of Science-Microbiology.

Dec 2007

Osmania University- Andhra Pradesh, India.

Bachelors of Science -Biotechnology, Botany, Chemistry.

Apr 2005

WORK EXPERIENCE:

Analytical Team Leader/Archivist

Burleson Research Technologies, Morrisville, NC.

Apr 2012-Present

. Review/Assignment of daily /weekly/monthly/yearly activities relevant

to analytical work and studies involving Immunoassay

Development/Validation/Implementation such as Clinical Trial

Immunoassay Analysis, ELISAs to Quantify Antibody Response to KLH or

SRBC, Host Resistance studies in mice and rats and ELISAs for Immune-

response to viruses such as Influenza, Streptococcus etc. in a GLP

environment

. Cell-culture and cell-based assays such as Interferon Bioassays, Viral

Plaque assays etc.

. Coordination of study analyses and interface with Study Directors and

QA.

. Scheduling, training, approvals and assignment of tasks.

. Organization, data entry and writing reports.

. Responsible for Archiving and Maintenance of Records.

Research Associate

Burleson Research Technologies, Morrisville, NC. Jul

2009- Mar 2012

. General Immunology / Biochemistry research and development.

. Observing GLP compliance for regulated studies.

. Responsible for adherence to study protocol, experiment and assay set-

up, quality control records of assay, and reporting of results.

. Hands on experience with mice. Various types dosing procedures on mice

such as oral gavage, subcutaneous injections, intra-parietal

injections, intra-venous injection, Local Lymph Node assays (LLNA)

etc.

Scientist

Becton Dickinson Technologies, RTP, NC.

Jun 2008-Jun 2009

. Immunoassays for Infectious Disease Testing

. Lateral Flow Assay for Infectious Disease Testing - Flu A/B and

Southern Hemisphere Clinical Feasibility Study.

. No wash assay development for Chlamydia trachomatis using SERS

Technology (Surface Enhanced Raman Spectroscopy).

. BSL1 and BSL2 infectious diseases and developing rapid diagnostic

tests for Influenza virus,Chlamydia trachomatis, Gonorrhea and

Trichomonas using SERS Technology

Research Assistant

Biological and Agricultural Engineering Department. NCSU

Jan 2008-May 2008

. Literature review on production of polyhydroxyalkanoates (PHA) by

microbial fermentation under Dr. Ratna Sharma, Biological and

Agricultural Engineering.

. Production of lipase enzyme from Aspergillus and Candida species under

Dr. Ratna Sharma, Biological and Agricultural Engineering.

Teaching Assistant

Summer College in Biotechnology and Life Sciences, i.e.SCIBLS.

North Carolina State University.

Summer 2007

Research Assistant/Support

Plant tissue culture project under Dr. Sivamani Elumalai,

Crop Science Department, North Carolina State University.

Nov 2006- Dec 2007

. Tissue culture and transformation in tobacco with Dr.Sivamani

Elumalai, Crop Science Department.

. Plant Tissue culture experiments like transformation of Tobacco

involving tobacco seed sterilization, media preparation, GUS assay,

MUG assay.

. Transformation experiments using Gene gun .Transformation of Rice by

Agro bacterium.

LABORATORY SKLLS:

Molecular Biology: Cloning experiments like cloning and successful

expression of green fluorescent protein, gfp in E.coli. TA cloning

experiments using TOPO cloning methodology. Cloning using PCR. Molecular

biology techniques like ELISA, Southern Blotting, and Western Blotting and

gel electrophoresis. Polymerase chain reaction and its applications

including Forensic PCR, Sequencing, RT-PCR, QPCR

Biochemistry: Protein Purification using ion exchange chromatography,

affinity chromatography, SDS-PAGE, FPLC, spectrophotometry (UV), Raman

spectroscopy and SERS Technology (BD Technologies)

Plant Biotechnology: Plant Tissue culture experiments like transformation

of Tobacco involving tobacco seed sterilization, media preparation, GUS

assay, MUG assay. Transformation experiments using Gene gun .Transformation

of Rice by Agrobacterium.

Animal Experience: Handling rats and mice in the vivarium. Various dosing

procedures in mice such as oral gavage, subcutaneous injections, intra-

parietal injections, intra-venous injection.

EDUCATION:

Graduate Certificate program in Molecular biotechnology:

. Manipulation and expression of Recombinant DNA.

. Detailed study of PCR and its applications in Forensics, RT-PCR,

Sequencing.

. Industrial Microbiology and Bioprocessing, protein purification.

. Production and expression of recombinant green fluorescent protein in

Escherichia coli with

Dr. Susan Carson, Biotechnology program.

Graduate Course Work:

. Medical microbiology including study of various microbial diseases.

. Chemotherapy and diagnosis.

. Immunology involving types of immune responses, antigen-antibody

reactions and cell-signaling systems.

. Food and Industrial microbiology involving sources, enumeration of

microbes in food.

. Fermentation process, culture systems and purification of fermented

products.

Under-Grad Course Work:

. Biotechnology: Basics of Biotechnology. Animal biotechnology including

study of production of vaccines, hybridoma technology. Plant

biotechnology and its applications in production of transgenic plants,

production of pharmaceuticals, food and beverages, metabolites.

Microbial and environmental biotechnology involving fermentative

production of microbial enzymes and antibiotics, microbial treatment

of municipal and industrial effluents.

. Botany: Taxonomy. Herbarium. Detailed study of medicinal plants. Plant

Physiology and metabolism. Plant biotechnology.

. Chemistry: Organic, Inorganic and Analytical Chemistry. Medicinal

chemistry including preparation of drugs like Aspirin, Ibuprofen etc

Undergraduate Project:

. Mushroom Cultivation.

ACTIVITIES:

. Proficiency certificate in Botany during Bachelors degree .2004-2005.

. Participated and presented a paper in "SYMBIOTECH- Workshop on

Molecular and Biochemical Techniques".

. Certificate in Fundamentals of Information Technology (Word, Excel,

PowerPoint).2003-2004.

. Proficiency certificate in Zoology.2002

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