Senior Scientist

Jin Chu, M.S., Ph.D.

* **** ****** **. ****, DE19701

267-***-**** (cell)

**************@*****.***

SUMMARY

Experienced in developing, validating, implementing, troubleshooting and reviewing ELISA, MSD, Luminex, and Flow Cytometry based immunoassays.

Experienced in creating, modifing, and improving SOPs, validation protocols and reports, sample analysis reports.

Experienced in developing and analyzing immunoassays in a Good Laboratory Practice (GLP) and Good Clinical Practice (GCP) compliant laboratory environment.

PROFESSIONAL EXPERIENCE

02/17- 10/17

Senior Research Scientist

TDM Pharmaceutical Research, LLC

100 Biddle Ave, Suite 202

Newark, DE 19702

09/11- 01/17

Senior Scientist

Center for Translational Medicine, Temple University

Philadelphia, PA 19140

5/08 - 8/11

Postdoctoral Researcher

Department of Pharmacology, Temple University

Philadelphia, PA 19140

01/00 - 12/07

Associated Scientist

Pharmacology Research Center, Xuanwu Hospital

Beijing, China, 100053

09/95 - 12/99

Assistant Scientist

Pharmacology Research Center, Xuanwu Hospital

Beijing, China, 100053

CURRENT RESPONSIBILITIES

Principal Investigator/Study Director

Responsible for the overall conduct of a laboratory study as defined by the Company SOP’s.

Assay Development and Validation

Develop, optimize, validate and execute assays for sample extraction procedures and biomarker analysis to support specific projects

Sample Analysis

Provide quantitative analyses of biological study samples.

Collect, calculate and analyze data.

Troubleshoot, maintain and operate lab insruments.

Write and review final validation and sample analysis reports.

Documentation

Plan, write study protocols, compile and interpret data/results, statistically analyze, summarize reports, monitor study progress, identify critical issues and solve problems

Ensure the proper maintenance of daily operation records.

Implement the performance and documentation of the proper inspection, calibration and maintenance for the RT-PCR instrument.

Management

Mentor and train junior scientists on clinical studies.

Manage and monitor daily activities of the assigned projects.

Lead technical staff for conducting GLP studies based on FDA guidelines.

EDUCATION

Institution Years Field Degree

Capital Medical University (CMU) 9/2004 - 5/2007 Neurobiology Ph.D.

Capital Medical University (CMU) 9/2000 - 5/2003 Pharmacolgoy M.S.

Peking University 9/1991 - 5/1995 Pharmaceutical Chemistry B.S.

School of Pharmaceutical Sciences (SPS)

PERSONAL SKILLS & COMPETENCIES

Self motivated professional with over twenty years of progressive experience in Research and Development projects.

Advanced experience in creating new research protocols, supporting ongoing studies and compiling data for preparing study reports.

Took responsibility for project outcome and monitored all aspects of operation, and coordinated the work of other staff, developed plans, and analyzed progress.

Demonstrated ability to work effectively both independently and collaboratively in a dynamic, team-oriented matrix environment.

Good presentation and communication skills.

SKILLS/EXPERTISE HIGHLIGHTS

Good laboratory practice (GLP), Good clinical practice (GCP), and Standard operating procedure (SOP).

Immunoassay: recombinant protein expression and purification, ELISA, MSD, Luminex assay, flow cytometry, immunoprecipitation and pull-down assays, radioimmunoassay, protein analysis and immunodetection by SDS/PAGE and 2D gel.

Cell biology: cell culture, generation of stably and transiently transfected cell lines, DNA and RNA transfection, RNAi (with siRNA, shRNA and lentivirus) or dominant negative interference, flow cytometry, immunofluorescence(IF), immunohischemistry(IHC), immune-electron microscopy, live cell imaging, cell lysis techniques, multiwall plate-based cytotoxicity and proliferation assays.

Molecular biology: gene and recombinant DNA cloning, genomic PCR, RT-PCR(Dye-Based and Probe-Based), sub cloning to expression vectors, DNA gel electrophoresis, DNA and RNA extractions, plasmid construction, Chromatin Immunoprecipitation (ChIP), plasmid preps, luciferase reporter assay, Transcription/Translation assay, in-situ hybridization.

Hitology: Frozen tissue and paraffin block preparation, sectioning, histological and immunohistochemistry or immunoflurescence staining for both frozen and paraffin sections.

Instrumentation: MSD multiplex system, Luminex immunoassay multiplexing analyzer, flow cytometry, fluorometer and plate reader, RT-PCR, laser confocal microscopy, fluorescence microscopy, HPLC, GC.

HONORS AND AWARDS

2013 AAQI research grant winner for 2013, Philadelphia, USA

2008 Grant fellowship of the 11th International Conference on Alzheimer Disease (ICAD), Chicago, USA

2008 Grant fellowship of the IXth World Conference on Clinical Pharmacology and Therapeutics (CPT), Québec City, Canada

SELECTED PUBLICATIONS (Total 68)

Chu J, Lauretti E, Praticò D. Caspase-3-dependent cleavage of Akt modulates tau phosphorylation via GSK3β kinase: implications for Alzheimer's disease. Mol Psychiatry. 2017 Jan 31. doi: 10.1038/mp.2016.214. [Epub ahead of print]

Chu J, Wisniewski T, Praticò D. GATA1-mediated transcriptional regulation of the γ-secretase activating protein increases Aβ formation in Down syndrome. Ann Neurol. 79(1):138-43(2016). PMID: 26448035

Chu J, Li JG, Hoffman NE, Stough AM, Madesh M, Praticò D. Regulation of gamma-secretase activating protein by the 5Lipoxygenase: in vitro and in vivo evidence. Sci Rep. 5:11086 (2015). PMID: 26076991

Chu J, Li JG, Hoffman NE, Madesh M, Praticò D. Degradation of gamma secretase activating protein by the ubiquitin–proteasome pathway. Journal of Neurochemistry. 133(3): 432-439 (2015). PMID: 25533523

Chu J, Li JG, Joshi YB, Giannopoulos PF, Hoffman NE, Madesh M, Praticò D. Gamma secretase-activating protein is a substrate for caspase-3: implications for Alzheimer's disease. Biol Psychiatry. 15; 77 (8):720-8 (2015). PMID: 25052851

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