Resume

Sign in

Protein Engineering

Location:
Lahore, Punjab, Pakistan
Salary:
30,000
Posted:
March 12, 2019

Contact this candidate

Resume:

BIO ***

*. Function Of Pedigree?

A pedigree may be used to establish the probability of a child having a particular disorder or condition. It may be used to discover where the genes in question are located (x, y, or autosome chromosome), and to determine whether a trait is dominant or recessive. 2. Define Epigenetics?

Epigenetics is the study of heritable phenotype changes that do not involve alterations in the DNA sequence

3. RAPD And ALFP Difference?

RAPD is Random Amplification of Polymorphic DNA'. It is a type of PCR, but the segments of DNA that are amplified are random.

AFLP is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering. Developed in the early 1990s by Keygene, AFLP uses restriction enzymes to digest genomic DNA, followed by ligation of adaptors to the sticky ends of the restriction fragments.

4. Point Mutation?

A point mutation or substitution is a genetic mutation where a single nucleotide base is changed, inserted or deleted from a sequence of DNA or RNA. Point mutations have a variety of effects on the downstream protein product—consequences that are moderately predictable based upon the specifics of the mutation. 5. Complications Of Inheritance?

Complications to inheritance patterns: Multiple genes - epistasis, polygenic traits Genes & the environment - sex-influenced traits, incomplete penetrance. Complications : A common recessive inheritnace can give dominant pattern, Autosomal dominant inheritance with non-penetrance, Autosomal dominant inheritance with variable expression.

6. Mutation Effects In Functioning Of Genes?

Some mutations don't have any noticeable effect on the phenotype of an organism. This can happen in many situations: perhaps the mutation occurs in a stretch of DNA with no function, or perhaps the mutation occurs in a protein-coding region, but ends up not affecting the amino acid sequence of the protein.

7. Causes Of Epigenetics?

BIO 301

Stress Hormone Causes Epigenetic Changes. Researchers found that chronic exposure to a stress hormone causes modifications to DNA in the brains of mice, prompting changes in gene expression

8. Genetic Mapping In Humans?

9. Forward, Reverse, And Neutral Mutations: 3marks Forward mutation: Mutation that alters the wild type phenotype into mutant phenotype. Reverse mutation: Mutation that changes a mutant phenotype back into the wild type. Neutral mutation: Mutation that alters the amino acid sequence of the protein but does not change its function as replaced amino acid is chemically similar or the affected AA has little influence on protein function

10. Point Mutations And Enlist 3 Structural Changes In Chromosomes? A point mutation or substitution is a genetic mutation where a single nucleotide base is changed, inserted or deleted from a sequence of DNA or RNA. Point mutations have a variety of effects on the downstream protein product—consequences that are moderately predictable based upon the specifics of the mutation. Deletion, duplication, translocation, transcription. 11. 5 Complications In Inheritance?

Epistasis, polygenic traits Genes & the environment - sex-influenced traits, incomplete penetrance.

12. Modification Of Epigenetica

Epigenetic modification - non coding RNA is a new mechanism for gene regulation. RNA which is not used for making proteins can be cleaved and used to inhibit protein-coding RNAs

(siRNAs, microRNAs). Three common types of modification - Cytosine methylation Histones modifications siRNAs

13. Mutation Alter Genes Expression?

14. RFLP And RAPD ?

RFLP is a technique that exploits variations in homologous DNA sequences. RFLP analysis was an important tool in genome mapping, localization of genes for genetic disorders, determination of risk for disease, and paternity testing. BIO 301

RAPD is Random Amplification of Polymorphic DNA'. It is a type of PCR, but the segments of DNA that are amplified are random.

15. Genetic Mapping?

Gene mapping describes the methods used to identify the locus of a gene and the distances between genes. The essence of all genome mapping is to place a collection of molecular markers onto their respective positions on the genome. 16. Sickle cell anemia?

Sickle cell anemia (sickle cell disease) is a disorder of the blood caused by an inherited abnormal hemoglobin (the oxygen-carrying protein within the red blood cells). The abnormal hemoglobin causes distorted (sickled) red blood cells. The sickled red blood cells are fragile and prone to rupture.

17. Point mutation and cause?

A point mutation or substitution is a genetic mutation where a single nucleotide base is changed, inserted or deleted from a sequence of DNA or RNA. Point mutations have a variety of effects on the downstream protein product—consequences that are moderately predictable based upon the specifics of the mutation. Cause:

The protein may lose its function, which can result ina disease in the organism. For example, sickle-cell disease is caused by a single point mutation (a missense mutation) in the beta- hemoglobin gene that converts a GAG codon into GUG, which encodes the amino acid valine rather than glutamic acid.

18. Mutation nomenclature?

Standard nomenclature based on genomic DNA reference sequences and RNA reference sequences is not shown. “Genomic DNA reference sequence” simply indicates any human DNA sequence in the database that is not based on a cDNA sequence. ... Example of standard mutation nomenclature based on a coding DNA sequence. 19. VTRN and application?

VNTR are short nucleotide sequences organized as a tandem repeats on genomes. Found on many chromosomes, Show variations in length between individuals. Applications of VNTR Microbiology DNA fingerprinting Genetic diversity Forensics Mapping of genomes Breeding patterns of wild or domesticated animals . 20.



Contact this candidate