Kaivon Assani 614-***-****
www.kaivonassani.com ac8dxj@r.postjobfree.com
SUMMARY
Developed Protocols and Medical Devices including electrospinning, imaging, DNA and protein analysis
in vitro, in vivo and ex vivo research to study disease progression in Cystic Fibrosis and gyrate atrophy of the choroid and retina
Statically Analyzed medical device and biomedical research using t-test, ANOVA and six sigma techniques.
Collaborated on projects with multiple groups and worked in tandem with large teams to conduct research for studies in CF metabolites, immunology and retinal human genetics
Publish intellectual property including 7 papers, 4 first authors, which have led to funding from multiple sources including the NIH and CF foundation.
Designed complex 3D model assemblies using Solidworks and FEA analysis (ex. bone locking plate, a TI 84 calculator)
Experimental Design including layout, planning, writing and paper submission.
EDUCATION
Master of Science in Biomedical Engineering, Biomaterials Focus July 2018
Wright State University, GPA 4.0 Dayton, Ohio
Six Sigma Certified November 2017
Institute of Industrial and Systems Engineers Dayton, Ohio
Bachelor of Science in Molecular Genetics December 2011
The Ohio State University Columbus, Ohio
EXPERIENCE (8+ years)
LAB
Materials Thesis Research: Artificial Skin Graft for Tissue Regeneration August 2016-August 2018
Wright State University Dayton, Ohio
Developed an electrospinning medical device for constructing wound patches by using a syringe pump loaded with a polymer (ex. Collagen), and collector plate spaced 18cm with a 37kV voltage potential.
Drug loading into electrospun wound patch, testing incorporation via FTIR
Statistical Data Analysis of qPCR data using t-test finding significant decrease in inflammatory macrophages due to mesenchymal stem cell (MSC) and retinoic acid drug treatment
in vitro Bioanalysis of MSC and drug treatment of macrophages testing qPCR, imaging and viability
Lab Manager/Research Associate: Biomedical Research July 2012-August 2016
The Research Institute at Nationwide Children’s Hospital Columbus, Ohio
Optimized and Established over 40 protocols including western protein analysis, qPCR DNA analysis, imaging, and biofilm analysis
Managed the Technical Development and research for all projects including CF immunology, device usage, and drug treatment; and trained all new lab members
in vitro and ex vivo Research using macrophages, neutrophils, human lungs and bronchoalveolar lavage for the treatment of Cystic Fibrosis (CF)
Statistical Analysis of all research projects using t-tests including qPCR, protein levels, cytokine levels and bacteria-autophagosome colocalization during IFN-γ treatment of CF macrophages.
Presented research at local and international conferences.
Collaborated to determine CF serum metabolite concentration via NMR
Kaivon Assani P2
Improved Efficiency of protocols, Examples:
- Decreased Time of image analysis for counting of bacteria and protein colocalization from a manual procedure taking 30 hours to 5 hours by writing a macro in Image J
- Saved Time and Increased Data Acquisition by converting continuous monitoring of bacterial growth to an automated procedure
Research Assistant August 2010-July 2012
The Research Institute at Nationwide Children’s Hospital Columbus, Ohio
In-vivo and in-vitro testing on Xenopus laevis for gyrate atrophy of the choroid and retina
ENGINEERING
Improving Femoral Prosthesis by Using Additive Manufacturing Technologies May 2017-August 2017
Wright State University Dayton, Ohio
Applied Rule of Mixtures to a solidworks hip implant model to determine the porosity of Ti-64 which would have comparable material properties to bone.
Printed a 3D Model using fused deposition modeling (FDM)
Simulation and Finite Element Analysis to determine displacement of the porous implant and compare it to femoral displacement
Stress/Strain Analysis January 2017-April 2017
Wright State University Dayton, Ohio
Determined Unknown Materials via Tension Testing. Engineering stress and strain were plotted and material properties were determined using MATLAB including elastic modulus, toughness, ultimate strength, fracture and yield properties. These uncovered the material identities as HDPE and PET.
Tested Elasticity of cooked and raw chicken bones using Three Point Bend Test. The uncooked bones had a higher elastic modulus and lower ultimate strength than the cooked bones due to denaturing collagen which is responsible for the bones elasticity.
Tested Viscoelaciticity of foam using Creep and Relaxation Test using a Kelvin–Voigt model. The accumulation of stress on the spring-loaded action of the foam shows non-linear relationship between strain over time and stress over time.
Statistics August 2017-December 2017
Wright State University Dayton, Ohio
Analysis using Statistical Software JMP by SAS and Excel used to conduct a t-test for qPCR cellular characterization of macrophages showing effective M1 to M2 conversion by treatment with Retinoic Acid loaded in a Pullulan/Gelatin nanofiber scaffold.
Analysis using Graphpad Prism to conduct Mann-Whitney and ANOVA to examine how Interferon Gamma affects Cystic Fibrosis macrophage autophagy including analysis of CF patient demographics, qPCR and protein levels, protein/bacteria co-localization, LDH, and ELISA IL1B and IL-10.
Designed Flow Chart describing data analysis was written using Microsoft Visio.
Electrical Circuits August 2016-May 2017
Wright State University Dayton, Ohio
Measuring Electrical Components and Testing continuity using Labview Software with NI myDAQ including phase difference, frequency, voltage, resistance and current.
Design of High Pass and Low Pass Filter using Operational Amplifiers and examining cutoff frequency, gain, transfer function and cutoff frequency.
Design of Bipolar Junction Transistors with PNP and NPN configuration used to make half-wave rectifiers, full wave rectifiers, amplifiers and digital logic systems
Kaivon Assani Qualifications, Honors and Awards
QUALIFICATIONS
3D Modeling: Solidworks, Finite Element Analysis, 3D Printing
Computers & Programing: MATLAB, Image J, Wordpress, JAVA
Data Analysis & Statistics: JMP by SAS, GraphPad Prism, Excel
Mechanics: Tensile Test, 3 Point Bend Test, Shear loading, Failure Analysis, Multi-Component Physics
Electronics: LabVIEW, Function Generator, Filters, Rectifiers, BJT, Operational Amplifiers, Digital logic Systems
Biomaterials: Tissue Engineering, Material/Mechanical Properties, FTIR, Electrospinning
LEADERSHIP EXPERIENCE
Founder Spring 2018-Present
Biomedical Research Conference, Wright State University Dayton, Ohio
Founder and President Fall 2016-Spring 2018
Biomedical Research and Technology Association, Wright State University Dayton, Ohio
Co-Founder and Vice President Fall 2017-Spring 2018
Biomedical Engineering Society, Wright State University Dayton, Ohio
Grader Fall 2017
MATLAB, College of Engineering and Computer Science, Wright State University Dayton, Ohio
Writer Fall 2016
Science News, Wright State University Guardian Dayton, Ohio
HONORS, AWARDS, AND MEMBERSHIPS
Engineering Honor Society, Tau Beta Pi
Academic Honor Society, Phi Kapa Phi
Academic Honor Society, Golden Key
Association of Old Crows (AOC)
AOC Academic Scholarship
NCH Outstanding Research Assistant
NCH Academic Award
DHLRI 2nd place poster
OSU Scholarship
Buckeye Community Scholarship
Biomedical Engineering Society
Institute of Electrical and Electronics Engineers
Dean’s List
Publications
AR-13 reduces antibiotic-resistant bacterial burden in cystic fibrosis phagocytes and improves cystic fibrosis transmembrane conductance regulator function. Assani et al..; J Cyst Fibros. Oct, 2018.
Human Cystic Fibrosis Macrophages Have Defective Calcium-Dependent Protein Kinase C Activation of the NADPH Oxidase, an Effect Augmented by Burkholderia cenocepacia. Assani et al. J Immu. Jan 2017
Cysteamine-mediated clearance of antibiotic-resistant pathogens in human cystic fibrosis macrophages. Shrestha & Assani et al. PLOS ONE. Oct, 2017
Characteristics of invasive Acinetobacter species isolates recovered in a pediatric academic center. Jain et. al. BMC Infectious Diseases. July 2016
Aging is associated with hypermethylation of autophagy genes in macrophages. Khalil et al..; Columbus, Ohio. Epigenetics. May 2016
IFN-γ stimulates autophagy mediated clearance of Burkholderia cenocepacia in human cystic fibrosis (CF) macrophages. (Press Release) Assani et al..; PLOS ONE. May, 2014.