Scientific Researcher

ASHLEY GOSS

**** * **** ******, *****, Florida, *3607

C: 813-***-**** *******************@*****.***

Summary

● B. Sc. in Biochemistry and M. Sc. in Biology, with specialization in biochemistry, microbiology, and molecular biology. Sixteen years of laboratory experience as a Laboratory Assistant, Laboratory Technician, Research Assistant, Product Inspector, Biological Scientist and Scientific Researcher. Dedicated, reliable, fast learner, able to focus on priorities, organize workload, work under pressure and in a face paced environment to meet deadlines.

Skills and Techniques

● Chemistry:Preparing various solutions and growth media, titrations, filtrations, separations, distillations, reflux, GC, TLC, HPLC, spectrophotometery (UV, IR and NMR)

● Manufacturing:Quality control of medical devices, GMP, writing/updating SOPs, LEAN principles

● Microbiology: Aseptic and sterile techniques, plating methods (streak, spread, pour, replica), colony PCR, agar plates/liquid media preparation, restriction enzymes, plasmid

(design, cloning, expression), Agrobacterium mediated plant transformation, serial dilutions, stab cultures, bacterial staining

● Biochemistry and Molecular Biology:DNA extraction, plasmid DNA preparation, PCR, RT-PCR, Agarose Electrophoresis, SDS-PAGE, Western blots, protein isolation and purification, immunoprecipitation, phosphorylation assays with P32, cAMP assays, calcium assays, dose response curves for various drugs, cell based assays

● Molecular Biology: Transformations using plant binary vector systems, RNA extractions, cDNA synthesis, gene fusion system, histochemical assays, paraffin embedding and microtome sectioning, sequencing (designing primers, interpret sequencing chromatograms), radioisotope labelling

● Biology:Cell culture, work with various cell lines (HEK293T, HASM (Human Airway Smooth Muscle, CHW (Chinese Hamster Fibroblasts)), media preparation

● Clinical:Blood analysis, Benchtop Blood Gas Analyzers (ABLs), Spun Hematocrit, handheld point of care blood analysis systems

● Environmental:Laboratory Information Management System (LIMS), sample check-in, management and custody

● Experience with operating scientific laboratory equipment:Centrifuges, microscopes (stereo, compound and dissecting), nanodrop, thermal cyclers, flow hoods, fume hoods, autoclave, FlexStation Multi Mode Microplate Reader

● General Laboratory:Maintain laboratory consumables inventory, procurement, journal writing, presentations, experiment/assay design,development and troubleshooting

● Statistics:Able to interpret, analyze scientific data and results and use statistical methods

Education

Master of Science in Biology 2012

Carleton University – Ottawa, ON, Canada

Bachelor of Science in Biochemistry 2009

Carleton University – Ottawa, ON, Canada

Experience

Scientific Researcher

University of South Florida– Tampa, FL June 2018 to May 2019

● Responsible for managing the laboratory, procurement, training, stocking the lab, experimental design

● The study of the molecular basis of G-protein coupled receptor structure and function

● Genomic-based drug discovery, on the most recently identified bitter taste receptors on airway smooth muscle and showing that they act to bronchodilate, thus being a potential new therapy for asthma and COPD

● Work with various cell lines (HEK293T, HASM, CHW)

● Screening compound libraries (millions)

● Routinely perform SDS-PAGE, Western blots, immunoprecipitation, cAMP Catchpoint assays, plasmid DNA isolation, PCR, RT-PCR, phosphorylation assays with P32, Fluo-4 Direct Calcium assays

Biological Scientist February 2017 to June 2018

University of South Florida– Tampa, FL

● The study of the molecular basis of G-protein coupled receptor structure and function

● Genomic-based drug discovery, on the most recently identified bitter taste receptors on airway smooth muscle and showing that they act to bronchodilate, thus being a potential new therapy for asthma and COPD

● Work with various cell lines (HEK293T, HASM, CHW)

● Routinely performed SDS-PAGE, Western blots, immunoprecipitation, cAMP Catchpoint assays, plasmid DNA isolation, PCR, RT-PCR, phosphorylation assays with P32, Fluo-4 Direct Calcium assays Client Services Technician December 2016 to February 2017 Pace Analytical – Oldsmar, FL

● Responsible for check-in of client samples, sample management and processing

● Process incoming samples, inspect samples to ensure good condition, signing appropriate chain-of-custody documents to receive samples, logging information into Laboratory Information Management System (LIMS) and assigning laboratory numbers to all samples

● Checking sample pH and preservation on samples

● Routing paperwork to Project Managers

● Preparing shipment of samples to internal/external clients Test Engineering Product Inspector October 2015 to February 2016 Epocal – Ottawa, ON

● Product inspector for handheld blood analysis testing equipment

● Examined product before and after finished goods testing and participated in correlating the physical state of the product with its measured performance

● Developed organizational system to optimize efficiency for my team; both physically and electronically to increase work output and decrease hours. Finished Goods Quality Control Laboratory Technician April 2014 to October 2015 Epocal – Ottawa, ON

● Quality control testing of handheld point of care blood analysis equipment prior to release

● Made real time decisions during product manufacturing to optimize product

● Developed and implemented training and standard operating procedures Clinical Services Laboratory Assistant September 2013 to April 2014 Epocal – Ottawa, ON

● Preformed standard laboratory procedures, organized product testing for technicians, blood sample and solution preparation, purchasing of supplies, equipment maintenance, and cleaning of the clinical laboratory Research Assistant September 2009 to May 2012

Carleton University – Ottawa, ON

● Fundamental research on substrate specificities for alcohol-forming fatty acyl reductases (FARs) that can be used with metabolic engineering to produce high-value natural products, such as biofuels

● Eight genes encoding FAR enzymes (FAR1-FAR8) from the Arabidopsis thaliana genome were researched and the substrate specificities of seven of these FARs were assessed by heterologous expression in the yeast Saccharomyces cerevisiae and Gas Chromatography

Research Assistant May 2009 to August 2009

Agriculture and AgriFood Canada – Ottawa, ON

● Research on plant-pathogen interactions between Pseudomonas syringae and bop1, bop2 and bop1 bop2 mutants of Arabidopsis thaliana Publications

Pascal, S., Bernard, A., Deslous, P., Gronnier, J., Fournier-Goss, A.E., Domergue, F., Rowland, O., Joubès, J..CER1-LIKE1 defines a novel cuticular very-long-chain alkane-forming complex in Arabidopsis. Plant Physiology (2019). 179(2): 415-432. Chacon, M.G., Fournier, A.E., Tran, F., Dittrich-Domergue, F., Pulsifer, I.P., Domergue, F., Rowland, O. Identification of Amino Acids Conferring Chain-Length Substrate Specificities on Fatty Alcohol-Forming Reductases FAR5 and FAR8 from Arabidopsis thaliana. The Journal of Biological Chemistry (2013). 288(42):303**-*****.

Doan, T.T.P, Domergue, F., Fournier, A.E., Vishwanath, S.J., Rowland, O., Moreau, P., Wood, C.C., Carlsson, A.S., Hamberg, M., Hofvander, P. Biochemical characterization of a chloroplast localized fatty acid reductase from Arabidopsis thaliana. Biochimica Biophysica Acta (2012). 1821(9): 1244-1255. Certificates

● WHMIS Certified

● Centrifuge, Radiosafety, Compressed Gas, Bio-hazard Training

● Canadian Government Security Clearance: Level II (Secret)

● Valid Florida driver’s license

● Green Card holder (Will never require sponsorship)

Contact this candidate