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Microbiologist

Location:
Upper Darby Township, Pennsylvania, United States
Salary:
40,000
Posted:
October 25, 2018

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Resume:

Heaven A. Oliver-Kozup, Ph. D.

*** **** ******, ****** ****, PA 19026

Phone: 610-***-**** Email: ac7h4t@r.postjobfree.com CAREER OBJECTIVE:

Well-educated, published microbiologist seeking a position as a scientific regulatory writer, senior level microbiologist, or research assistant. SUMMARY OF QUALIFICATIONS:

Word processing skill set includes all Microsoft Office programs, ADOBE

More than 15+ years of experience in microbiology, astrobiology, and microbial pathogenesis research and course work

In depth knowledge of laboratory SOPs, techniques and diagnostic applications

Extensive microscopy experience (CLSM and FESEM) with analysis using multiple types of software including ImageJ, AutoQuant, LSM Imaging software

Proficient in DNA, RNA, and protein extraction, agarose and polyacrylamide gel electrophoresis and protein purification techniques

Skilled professional in many computer software and statistical analysis

Excellent organizational and problem-solving skills

Exceptional in designing experiments, writing/preparing/analyzing data for publications

EDUCATION:

Doctor of Philosophy in Microbial Pathogenesis. Department of Microbiology, Immunology and Cell Biology. College of Medicine. West Virginia University. Morgantown, WV (2013)

Master of Science in Biology/Microbiology. Department of Biological Sciences. University of Arkansas. Fayetteville, AR (2003)

Bachelor of Science in Biology. Division of Science and Mathematics. University of the Ozarks. Clarksville, AR. (2000)

PUBLICATIONS:

Oliver-Kozup, H. A., Martin, K. H., Schwegler-Berry, D. E., Green, B. J., Betts, C., Van De Water, L.; and Lukomski, S. (2013) Targeted binding of the surface adhesin Scl1 to a spliced variant of cellular fibronectin expressed in wounded tissue supports Group A Streptococcus biofilm formation. Mol Microbiol, 87:672- 89.

Oliver-Kozup H. A., Elliott, M., Bachert, B., Martin, K. H., Reid, S., Schwegler- Berry, D., Green, B. J., and Lukomski, S. (2011) The streptococcal collagen-like protein-1 (Scl1) is a significant determinant for biofilm formation by group A Streptococcus. BMC, 11:262.

Caswell, Clayton C., Oliver-Kozup, Heaven A., Han, Runlin, Lukomska, Ewa, and Lukomski, Slawomir. (2010) Scl1, the multifunctional adhesin of group A Streptococcus, selectively binds cellular fibronectin and laminin, and mediates pathogen internalization by human cells. FEMS Microbiol Lett. 303: 61-68. C.C.C and H.O-K. contributed equally to this publication.

Oliver-Kozup, Heaven A. and Kral, Timothy. (2007) “Methane Production on Rock and Soil Substrates by Methogens: Implications for Life on Mars”. Astronomical Society of the Pacific Conference Series. Bioastronomy: Molecules, Microbes and Extraterrestrial Life. 420: 137-146.

RESEARCH EXPERIENCE:

University of Oklahoma 2015-2016

Chemistry and Biochemistry

Position: Post-Doctoral Fellow

Overview: research focused on the toxin-antitoxin (TA) modules of Pseudomonas aeruginosa in identification of potential variations in the expression of TA genes during environmental stressors. BSL-1 and BSL-2 laboratories o RNA isolation and purification, RT-qPCR analysis (LightCycler Software), bacteriology, biohazard management

J. Craig Venter Institute 2013

Genomic Medicine

Position: Post-Doctoral Fellow

Overview: research focused on identifying dynamic relationships between commensal bacteria and multi-drug resistant bacteria during combat wound infections. BSL-2 laboratory

o RNA isolation and purification, MDR bacterial isolation/growth analysis, eukaryotic cell culture, genetic sequencing, bioinformatics, electrophoresis

West Virginia University 2007-2013

Microbiology/Immunology/Genetics/Molecular Biology Position: Graduate Research/Teaching Assistant

Overview: research focused on Group A Streptococcus (GAS) genetics and virulence factors with emphasis on the streptococcal collagen-like proteins in GAS biofilm formation and host colonization during cicatrisation; BSL-2 level laboratory

o Eukaryotic cell culture, fluorescent microscopy, confocal laser scanning microscopy (CLSM), field emission election microscopy (FESEM), DNA/plasmid isolation and purification, protein purification, flow cytometry (FACS), molecular cloning, PCR, chromatographic techniques, restriction/digestion techniques, ELISA, biofilm analysis, electrophoresis, immunoblotting

Thomas Jefferson University 2004

Molecular Biology/Pathology

Position: Research Associate

Overview: research focused on synthesis of siRNA for inhibition of HMG-CoA reductase in the biosynthetic pathway of cholesterol o Eukaryotic cell culture, RNA isolation and purification, molecular techniques

University of Arkansas 2000-2003

Astrobiology/Microbiology

Position: Graduate Research/Teaching Assistant

Overview: research focused on Archaea with emphasis on methanogenic growth in various soil substrates

o Anaerobic archaeal cell culture, soil/media sample preparations, gas chromatography

University of Arkansas 1998

Microbiology

Position: Undergraduate Research fellow

Howard Hughes Medical Institute Undergraduate Research Program Overview of work: research focused on Archaea and the inhibition of methanogenic growth in medium. Presentation title: “Methanogen: The Quest for

“Methanocin”

ACADEMIC APPOINTMENTS and HONORS/AWARDS:

2012 Conference Moderator - Pittsburgh Bacterial Meeting, Pittsburgh, PA – presentation title “The targeted binding of Scl1 to a spliced variant of cellular fibronectin expressed in wounded tissue”

2008-2012 Doctoral Fellow, WV EPSCoR STEM Cancer Nanotechnology

2010 Awardee (First Place), Van Liere and Research Day, WVU - poster title

“The Streptococcal collagen-like protein-1 (Scl1) is an essential adhesin for biofilm formation and pathogenesis for Group A Streptococcus”

2010 Awardee (Second Place) - STaR Symposium, Huntington, WV - poster title “The Streptococcal collagen-like protein-1 (Scl1) is a key surface adhesin for biofilm formation of group A Streptococcus”

CONFERENCE PRESENTATIONS:

2013 Heaven A. Oliver-Kozup, Jessica DePew, Maria Kim. George Mason University – poster title “Analysis of multi-drug resistant microbial interactions during biofilm formation in wounded warriors”

2012 Heaven A. Oliver-Kozup, Karen H. Martin, Diane Schwegler-Berry, Brett J. Green, Livingston Van De Water, and Slawomir Lukomski. Van Liere and Research Day, WVU – poster title “Scl1 targeted binding to a spliced variant of cellular fibronectin expressed in wounded tissue”

2012 Heaven A. Oliver-Kozup (Conference Moderator) and Slawomir Lukomski. Pittsburgh Bacterial Meeting, Pittsburgh, PA – presentation title “The targeted binding of Scl1 to a spliced variant of cellular fibronectin expressed in wounded tissue”

2011 Heaven A. Oliver-Kozup, Courtney Betts, Livingston Van De Water and Slawomir Lukomski. 6

th

International Conference on Gram-Positive

Microorganisms, 16

th

International Conference on Bacilli, Montecatini, Italy – presentation title “Targeted binding of the surface adhesin Scl1 to a spliced variant of cellular fibronectin expressed in wounded tissue”

2011 Heaven A. Oliver-Kozup, Courtney Betts, Livingston Van De Water and Slawomir. Van Liere and Research Day, WVU – poster title “Scl1 targeted binding to a spliced variant of cellular fibronectin expressed in wounded tissue”

2011 Heaven A. Oliver-Kozup, Karen H. Martin, Diane Schwegler-Berry, Brett J. Green, and Slawomir Lukomski. The 6

th

Mid-Atlantic Microbial Pathogenesis

Meeting, Wintergreen, VA – poster title “The streptococcal collagen-like protein- 1 (Scl1) is a key surface adhesin for biofilm formation by group A Streptococcus”

2010 Heaven A. Oliver-Kozup, Amanda Suchanek, and Slawomir Lukomski. Van Liere and Research Day, WVU – (Awardee -1

st

place) poster title “The

Streptococcal collagen-like protein-1 (Scl1) is an essential adhesin for biofilm formation and pathogenesis for Group A Streptococcus”

2010 Heaven A. Oliver-Kozup, Karen H. Martin, Diane Schwegler-Berry, Brett J. Green, and Slawomir Lukomski. STaR Symposium, Huntington, WV –

(Awardee 2

nd

place). poster title “The Streptococcal collagen-like protein-1 (Scl1) is a key surface adhesin for biofilm formation of group A Streptococcus”

2010 Heaven A. Oliver-Kozup and Slawomir Lukomski. Pittsburgh Bacterial Meeting, Pittsburgh, PA – presentation title “Contribution of the Streptococcal collagen-like protein-1 (Scl1) in Group A Streptococcus biofilm formation”

2009 Clayton Claswell, Heaven A. Oliver-Kozup, Ewa Lukomska, and Slawomir Lukomski. 109

th

General Meeting American Society for Microbiology, Philadelphia, PA – poster title “Streptococcal Collagen-like Protein-1 (Scl1) Mediates Adhesion to Selected Extracellular Matrix Components and GAS internalization”

2009 Clayton Claswell, Heaven A. Oliver-Kozup, Ewa Lukomska, and Slawomir Lukomski. Van Liere and Research Day, WVU – poster title “Scl1 Mediates Adhesion to Selected Extracellular Matrix Components and GAS Internalization”

2009 Clayton Claswell, Heaven A. Oliver-Kozup, Ewa Lukomska, and Slawomir Lukomski. WV Nano Research Symposium, WVU – poster title

“Streptococcal Collagen-like Protein -1 (Scl1) Mediates Adhesion to Selected Extracellular Matrix Components and GAS Internalization”

2009 Clayton Claswell, Heaven A. Oliver-Kozup, Ewa Lukomska, and Slawomir Lukomski. STaR Symposium, Charleston, WV – poster title

“Identification and Characterization of Binding between the Streptococcal Collagen-like Protein-1 (Scl1) of Group A Streptococcus and Extracellular Matrix Components”

2009 Clayton Claswell, Heaven A. Oliver-Kozup, Ewa Lukomska, and Slawomir Lukomski. The 5

th

Mid-Atlantic Microbial Pathogenesis Meeting,

Wintergreen, VA – poster title “Streptococcal collagen-like protein (Scl1) of group A Streptococcus binds selected extracellular matrix components”

2009 Clayton Claswell, Heaven A. Oliver-Kozup, Ewa Lukomska, and Slawomir Lukomski. Pittsburgh Bacterial Meeting, Pittsburgh, PA – poster title

“The Streptococcal collagen-like protein (Scl1) of group A Streptococcus selectively binds Extracellular Matrix proteins, Laminin and Cellular Fibronectin”

2008 Clayton Caswell, Runlin Han, Heaven A. Oliver-Kozup, and Slawomir Lukomski. Pittsburgh Bacterial Meeting, Pittsburgh, PA – presentation title

“Immune evasion by binding of complement factor H to the collagen-like surface protein Scl1 of Streptococcus pyogenes”

PROFESSIONAL EXPERIENCE:

Environmental Safety Manager: 2005 – 2007

Gwynedd-Mercy College

Overview: College-wide development of EPA/OSHA compliance management and safety programs including online safety courses and hands-on safety training

Natural Science/Mathematics Department Laboratory Manager: 2005 – 2007 Gwynedd-Mercy College

Overview: Supervisory position over natural sciences laboratory management; organization of science laboratory instruction for employed students; scheduling and safety training; department budget and scientific equipment management TEACHING EXPERIENCE:

Adjunct Professor

Gwynedd-Mercy University 2017

Undergraduate Education: Microbiology

Teaching Assistant

West Virginia University 2007-2009

Undergraduate/Graduate Education: Microbiology

Adjunct Faculty

Gwynedd-Mercy College 2005-2007; 2017

Undergraduate Education: Microbiology and Immunology

Adjunct Faculty

Montgomery County Community College 2003-2005

Undergraduate Education: Microbiology and Immunology

Adjunct Faculty

Community College of Philadelphia 2003

Undergraduate Education: Clinical Microbiology

Teaching Assistant

University of Arkansas 2000-2003

Undergraduate Education: Biology Laboratory Techniques Undergraduate Education: Microbiology Laboratory Techniques WVU UNIVERSITY SERVICE AND AFFILIATIONS:

Member: Van Liere Organization Committee

Research Fellow: Science, Technology, Engineering and Mathematics (STEM) Coalition

CURRENT AND PREVIOUS MEMBERSHIP IN PROFESSIONAL

ASSOCIATIONS

American Society for Microbiology (ASM)

Eastern Pennsylvania Branch of American Society for Microbiology (EPASM)

The National Science and Mathematics Honor Society (Sigma Zeta)



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