Letitia Beckett
**** ****** **** ******* ****, Oxford, NC 27565
ac7gla@r.postjobfree.com Cell 919-***-****
Research Experience
Research Assistant/Graduate Student, North Carolina State University, 2016-2017
Principal Investigator: Dr. Johanna Elfenbein/Dr. Belinda Akpa
Examined Salmonella Growth Curves.
Examined mathematical modeling of biological systems with the use of MATLAB.
Research Assistant/Graduate Student, North Carolina A&T State University, 2014-2017
Principal Investigator: Dr. Robert Newman/Dr. Elimelda Ongeri
Investigated the impact of post-translational modifications on protein kinase function
Examined the impact of meprin A-mediated cleavage of the catalytic subunit of Protein Kinase A Cβ1 (PKA Cβ1) on its function
Investigated PKA CB1 substrate selection before and after meprin A cleavage
Expressed proteins in yeast cells
Purified protein using a variety of purification techniques
Examined protein presence, protein activity, and protein purity
Analyzed Data
Examined the effects of cleavage on redox sensitivity in relation to oxidation
Research Assistant, Shaw University, 2011-2012
Principal Investigator: Dr. Ravindra Kumar
Independently examined the basic properties and principles of light and the mechanism of motion of light through fiber optic light pipes
Learned and developed an understanding of the concept with the use of its laws, theories and equations, and practicing understanding by completing problems
Concepts were applied to research determining the uses of fiber optic light pipes in medicine and biology
Research Assistant, Shaw University, 2009-2010
Principal Investigator: Dr. Mialy Rabe
Examined the airborne characteristics of three unidentified, gram-positive soil bacteria that were transported by shoes from Madagascar and South Africa
Collected samples
Cultured, incubated, counted and analyzed bacterial growth
Gram staining
Practiced aseptic techniques
Constructed experimental apparatus
Research Assistant, Shaw University’s Institute for Health Social and Community Research (IHSCR) and The University of the West Indies, Kingston, Jamaica 2009
Principal Investigator: Dr. Daniel Howard
Investigated the knowledge, attitude and practices of Jamaican men towards prostate cancer
Focused on the perceptions of Jamaican men surrounding prostate cancer and screening
Participated in cultural tours and interviews with Jamaican residents
Participated in discussions, lectures, and reading on prostate cancer by leading researchers, healthcare providers, community advocates, and professors
Obtained some training in survey data collection and data analysis
Administered several oral presentations both individual and group throughout the program
Technical Skills
Protein Purification Techniques (Low throughput GST protein purification from yeast, Low throughput Ni-NTA protein purification from yeast, p6 column chromatography)
SDS-PAGE Western Blotting
Silver Staining Method
Coupled ATP depletion assays ( Kinase GLO Phosphorylation Assay)
Protein Microarray Analysis
Redox reactions (diamide and hydrogen peroxide)
Cloning/Subcloning
DNA, RNA, and protein extraction, gel electrophoresis, and immuno-blotting procedures
Cell tissue culture procedures, Agar Culturing, Conventional Polymerase Chain Reaction, Cell separation techniques
Education
Shaw University, Raleigh, North Carolina
Bachelor of Science
Major: Biology
Received: May, 2012
GPA: 3.9/4.0
Presentations
Experimental Biology Conference, San Diego, CA, April 2016- Poster
“The Impact of Meprin A-Mediated Cleavage on the Function of the Catalytic Subunit of Protein Kinase A (PKA Cβ1)” - Poster
Emerging Researchers National Conference in STEM, Atlanta, GA, 2012
“Fiber Optic Light Pipes and Probes in Medicine and Biology” - Oral
Emerging Researchers National Conference in STEM, Washington, DC, 2010
“Unidentified Gram -Positive Soil Bacteria Transported by Shoes from Madagascar and South Africa May be Airborne” - Poster
Minority Access Inc., Tenth Annual Role Models Conference, Washington, DC, 2009
“Controversies and Challenges of Prostate Cancer”- Oral
Honors and Awards
Rising Star Award at Shaw University National Alumni Association (NAA) Legends Ball, 2017
Attended North Carolina State University College of Veterinary Medicine, Raleigh, North Carolina; Major: Comparative Biomedical Sciences, PhD
Concentration: Immunology
2016-2017
NIH Initiative for Maximizing Student Diversity(IMSD) scholar at NC State University, Fall 2016- Summer 2017
Attended North Carolina A&T State University, Greensboro, North Carolina; Master of Science; Major: Biology
2014-2017
NIH Research Initiative for Scientific Enhancement (RISE) scholar at NC A&T State University, Fall 2014- Summer 2017
Activities
Secretary of the Shaw University Granville, Vance, and Warren Alumni Association