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Assistant Manager

Charlottesville, Virginia, United States
August 30, 2018

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Shivani Kalia

****B Commonwealth Dr

Charlottesville, VA 573-***-****



• Microbiological analysis under GLP/GMP guidelines

• Activity analysis of feed enzymes

• Conducted research on enzyme kinetics

• Primer designing, molecular cloning and assay development

• Scientific writing experience with publications, SOPs and research proposals Experience Novus International, Inc. St. Charles, MO Biochemist April 2018 – July 2018

• Analyzed animal feed additives involved in improving the animal digestibility and nutrient availability

• Worked on the evaluation of thermostability of proteases in formulated products and animal feed

• Performed method development through the modification of enzyme assays

• Performed time dependent enzyme assay for phytase in both product and feed

• Evaluation of activity of proteolytic enzymes using casein as a substrate

• Enumeration of bacillus spores in animal feed and probiotic products Missouri University of Science and Technology Rolla, MO Teaching Assistant Aug 2016 – June 2017

• Microbiology Lab

Missouri University of Science and Technology Rolla, MO Research Assistant Aug 2015 – June 2017

• Growing and characterizing microorganisms.

• Production of 1,3-propanediol (PDO) from glycerol, a biodiesel waste product, using a haloalkaliphilic bacterium, Halanaerobium hydrogeniformans

• Performed media screening tests to identify the factors that have significant impact on PDO production

• Performed batch reactor studies to determine the rate of PDO production as a function of reactor volume

• Performed repeated fed batch reactor operations to maximize the productivity

• Worked on enzyme kinetics studies for the investigation of metabolic fluxes involved in PDO production

• Performed PCR for gene amplification from the bacterial genome and SDS PAGE for protein expression analysis

• Enzyme assays on purified enzyme obtained through Histidine tagging

• Maintained detailed laboratory notebooks in compliance with laboratory requirements and university guidelines

• Developed and implemented standard operating procedures and protocols for enzyme assays

Amity University Noida, India

Internship Dec 2012 - May 2013

• Evaluation of Immunomodulator activity of drugs in cell line and Zebra fish model

• Performed various enzymatic and immunomodulatory tests

• Evaluated drug efficacy on the basis of pharmacodynamics protein profiling Indian Institute of Integrative Medicine Jammu, India Genetic Diversity Analysis Jan - Jul 2011

• Performed PCR to analyze the genetic diversity of different ginger accessions Shivani Kalia

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Education Missouri University of Science and Technology Aug 2015 – June 2017 M.S. Environmental Microbiology GPA: 4.00/4.00


Bioremediation Geomicrobiology

Biostatistics Molecular Genetics

Scanning Electron Microscopy Problems in Applied & Environmental Biology Research Proposal Writing

Amity University Aug 2011 – June 2013

M.Tech Biotechnology


Immunology Proteomics

Cell Biology Pharmacology

Enzyme technology Microbiology

Genetic Engineering Biochemistry

Technical Skills Enzyme Assays Polymerase Chain Reaction (PCR) HPLC Electrophoresis Plasmid purification SOP writing

SDS PAGE Scanning Electron Microscope Statistical Data Analysis Fermentation Anaerobic Culture Techniques Immunoassays Molecular Cloning

Publications “The use of a fractional factorial design to determine the factors that impact 1,3- propanediol production from glycerol by Halanaerobium hydrogeniformans.” S. Kalia, J. Trager, O.C. Sitton, M.R. Mormile, Life (Basel) 6 (3) Aug 20, 2016

“1,3-propanediol production from glycerol by Halanaerobium hydrogeniformnas, a haloalkaliphilic bacterium, under scale up reactor and repeated fed batch operations.” S. Kalia, D. Roush, D. Courtney, O.C. Sitton, M.R. Mormile, "in preparation"

Conferences Amity University, India

• National Symposium on “Immune Mechanism In Host Pathogen Interactions: Emerging Trends” and workshop on “Flow Cytometry And Q-PCR: Principles and Clinical Applications” Sep 27-28, 2012

• National Workshop on Advanced Analytical Techniques in Research and Development. Dec 20–21, 2012

• International Conference on Biodiesel Production, Dec 22, 2012 Hamdard University, India

• Presented poster on “Evaluation of protein signatures in type 2 diabetes mellitus” The National Seminar on Proteomics based Biomarkers for Animal and Plant Disease, March 1-2, 2013

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