Histotechnologist

C U R R I C U L U M V I T A E

Ran Gu

**** ******* ****, ********, **, USA

Mobile: 650-***-****

Email: ***.*.**@*****.***

Professional Statement

I graduated from Monash Medical Centre, Monash University in Melbourne, Australia, with a PhD in Biomedical science field. I have been working as a postdoctoral researcher in University of California, Davis for last 5 years. During my PhD and postdoctoral training, I have gained extensive experiences in histological and pathological analysis. I’m familiar with operating the automatic tissue processor, routine histological processing and decalcification processes, and both paraffin and frozen block sectioning. I have almost 10-years experiences of routine and special staining, immunohistochemistry staining, immunofluorescence staining, in-situ hybridization, imaging analysis, laser capture, RNA-Seq, Western-Blotting, and quantitative and qualitative PCR. Furthermore, I have much more experience in Excel, GraphPad Prism, PowerPoint and MS word.

Experience

Dec 2015-present Postdoctoral Researcher

Zhou Lab, Department of biochemistry and molecular medicine, University of California, Davis

Mouse embryonic tissues collection, operating automatic processor, and making frozen and paraffin blocks

Section paraffin and frozen blocks with microtone and cryostats

Performing H&E staining, immunohistochemistry staining, immunofluorescence staining and in-situ hybridization

Genotyping

Extract DNA/RNA and perform RNA-seq, PCR, q-PCR and Western Blotting

Cell culture

Bacterial culture

Maintaining pathological equipment, including the stereoscopes and fluoresces microscopes

Analyze and interpret research results with multiple programs, including Excel and GraphPad Prism

Mentor undergraduate and graduate students

2013-April 2015 Postdoctoral Researcher

Adamopoulos Lab, Department of internal medicine, University of California, Davis

Mouse tissues collection, and operating automated processor

Decalcification of mouse ankles and long bones, and making both frozen and paraffin blocks

Section paraffin and frozen blocks with microtone and cryostats

Performing H&E staining, Safranin-O staining, TRAP staining, immunohistochemistry staining, and immunofluorescence staining.

2009 – 2013 Doctor of Philosophy

Department of Medicine, Monash Medical Centre for Inflammatory Diseases

Monash University

Mouse arthritis model induction and monitoring, and collection of normal and inflammatory mouse tissues

Decalcification of mouse ankles

Operating of automated processor, and make both frozen and paraffin blocks

Section paraffin and frozen blocks with microtone and cryostats

Performing antigen retrieval on paraffin sections

Performing H&E staining, Safranin-O staining, TRAP staining, immunohistochemistry staining, and immunofluorescence staining.

MicroCT scanning and imaging of un-decalcified mouse long bones

Extract DNA/RNA and perform RNA-seq, PCR, q-PCR and Western Blotting

Cell culture

Analyze and interpret research results with multiple programs, including Excel, GraphPad Prism

Mentor undergraduate students

Educations

2008 – 2009 Bachelor of Biomedical Science, Honours

Department of Medicine, Monash Centre for Inflammatory Diseases

Monash University

2007 - 2008 Advanced Diploma of Translating

Australian Institute of Translation and Interpretation

2004 – 2007 Bachelor Biomedical Science

Monash University

2002 – 2004 Victorian Certificate of Education

Strathcona Baptist Girls Grammar School

Certificate

1.Histotechnologist (ASCP)CM 7128

Major Skills

1.HE staining, Immunofluorescence and immunohistochemistry (10 years’ experience)

2.In situ hybridization (3 years’ experience).

3.Fluorescence microscope, confocal microscope, and image analysis (10 years’ experience)

4.Animal disease model induction and monitoring (10 years’ experience)

5.Transgenic mice analysis (Genotype, collecting tissue for morphology, mRNA and Western-blot) (10 years’ experience)

Honours

2016 – present Shriners’ Hospital Postdoctoral Research Fellowship

Department of Medicine, UC Davis

2012 – 2013 Monash Departmental PhD Scholarship

Department of Medicine, Monash University

2012 Felix Bronner Young Investigator Award

American Society of Bone and Mineral Research (ASBMR)

2012 Department Achievement Award

Department of Medicine, Monash University

2010 – 2012 Ulyssse Club Australia Arthritis Research Scholarship

Ulyssse Club Australia

2010 Postgraduate travel Grant Award

Monash Research Graduate School

2009 – 2010 Faculty Postgraduate Research Scholarship (FPRs)

Faculty of Medicine, Nursing and Health Sciences, Monash University

2009 Department Achievement Award

Department of Medicine, Monash University

PUBLICATIONS

Odobasic D, Jia Y, Kao W, Fan H, Wei X, Gu R, Ngo D, Richard Kitching A, Holdsworth SR, Morand EF, Yang YH. Formyl peptide receptor activation inhibits the expansion of effector T cells and synovial fibroblasts and attenuates joint injury in models of rheumatoid arthritis. J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 29

Wu DJ, Gu R, Sarin R, Zavodovskaya R, Chen CP, Christiansen BA, Zarbalis KS, Adamopoulos IE. Corrigendum to "Autophagy-linked FYVE containing protein WDFY3 interacts with TRAF6 and modulates RANKL-induced osteoclastogenesis" J Autoimmun. 2017 Nov;84:132

Gu R, Santos LL, Ngo D, Singh P, Fan HP, Fingerle-Rowson G, Bucala R, Quinn JMW, Morand EF. Macrophage Migration Inhibitory Factor is essential for osteoclastogenic mechanisms in vitro and in vivo mouse model of arthritis. Cytokine. 2015 Jan 31;72(2):135-145

Gu R and Kao WP, (co-first author), Wei XM, Fan HP, Zhang ZY, Quinn JMW, Morand EF, Yang Y. Annexin A1 Receptor Agonist Suppresses Development of Inflammatory Arthritis, Br J Pharmacol. 2014 Sep;171(17):4087-96.

Fan HP, Ngo D, Gu R, Fingerle-Rowson G, Bucala R, Yang Y, Morand EF. Macrophage Migration Inhibitory Factor inhibits the anti-inflammatory effects of Glucocorticoid Induced Leucine Zipper. Arthritis Rheumatol. 2014 Aug;66(8):2059-70.

Ngo D, Beaulieu E, Gu R, Leaney A, Santos LL, Fan H, Yang YH, Kao W, Xu J, Escriou V, Loiler S, Vervoodeldonk MJ, Morand EF. Divergent effects of endogenous and exogenous GILZ in models of inflammation and arthritis. Arthritis Rheum. 2013 May;65(5):1203-12

Aradhana Upadhyay, Danielle Senyschyn, Leilani Santos, Ran Gu, Graeme J. Carroll, Jalal A. Jazayeri. K/BxN serum transfer arthritis is delayed and less severe in leukaemia inhibitory factor (LIF) deficient mice. Clinical & Experimental Immunology 2012 Aug;169(2):71-8

Lara J Herrero, Michelle Nelson, Anon Srikiatkhachorn, Ran Gu, Surapee Anantapreecha, Günter Fingerle-Rowson, Richard Bucala, Eric Morand, Leilani L Santos, Suresh Mahalingam Critical role for macrophage migration inhibitory factor (MIF) in Ross River virus-induced arthritis and myositis. National Academy of Sciences 07/2011; 108(29):12048-53.

Hasnawati Saleh, Damien Eeles, Jason M Hodge, Geoffrey C Nicholson, Ran Gu, Sueli Pompolo, Matthew T Gillespie, Julian M W Quinn. Interleukin-33, a target of parathyroid hormone and oncostatin m, increases osteoblastic matrix mineral deposition and inhibits osteoclast formation in vitro. Endocrinology 03/2011; 152(5):1911-22.

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