C Nd

Nicole J ennifer M edeiros

*** * ** * **** D rive

North W ales, P A 1 9454

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610-***-****

EDUCATION

Master o f S cience, M ay 2 016

Biomedical S ciences G raduate P rogram, D epartment o f M icrobiology a nd I mmunology Temple U niversity, L ewis K atz S chool o f M edicine, P hiladelphia, P A Thesis: “ I n V ivo E xpression o f t he B acterial A myloid C urli” Advisor: D r. C agla T ukel, A ssistant P rofessor o f M icrobiology a nd I mmunology GPA: 3 .54

Bachelor o f S cience, M ay 2 014

Wester C hester U niversity o f P ennsylvania, W est C hester, P A Nutrition M ajor, B iology M inor

GPA: 3 .875 S umma C um L aude

RESEARCH E XPERIENCE

The W istar I nstitute, P hiladelphia, P A

Research Assistant III, J une 2 016-Present

Duties: A ssist m embers o f t he l ab w ith v arious a ssays a nd p erform i nitial p ilot e xperiments i nvolving b iochemical evaluation o f c hromatin r emodelers

● Generated m ultiple s table c ell l ines f or u se i n c hromatin r emodeler e xperimentation

Cell l ine m aintenance, t ransfection, a nd c haracterization

● Utilized v arious t echniques f or i nitial c loning a nd c haracterization o f a n a ntibody f or g eneral u se i n t he l ab

● Characterized p rotein-protein c omplexes t hrough i mmunoprecipitation e xperiments.

● Developed a l ibrary o f r ecombinant c onstructs t hrough m olecular c loning t echniques

● Expressed a nd p urified p roteins f or a ntibody d evelopment a nd i n v itro s tudies

● Developed B acmid D NA f or u se i n B aculovirus g eneration f or S F9 c ells 1

N. J . M edeiros, M .S.

Temple U niversity, P hiladelphia P A

Graduate S tudent R esearcher, L ewis K atz S chool o f M edicine, 2 014-2016 Project: D etermining t he i n v ivo e xpression o f a b acterial a myloid d uring b acterial i nfection.

● Utilized m ultiple i n v ivo a nd i n v itro i mmune f unction a ssays t o m easure h ost r esponse t o b acterial a myloids

● Used a S almonella i nfection m odel o f l ong-term t yphoid i nfection i n m ice

● Collaborated w ith D r. S tefania G allucci, T emple U niversity i n h er r esearch r egarding S ystemic L upus Erythematosus ( SLE)

RESEARCH E XPERIENCE C ONTINUED

West C hester U niversity o f P ennsylvania, W est C hester, P A Volunteer S tudent R esearcher, D epartment o f V irology, 2 013-2014 Project: P articipated i n o ngoing r esearch f ocusing o n t he d iscovery o f n atural a ntiviral d rugs

● Screened p lants w ith c urative p roperties f or a ntiviral a ctivity u sing V accinia a nd h erpes s implex a s i ndicator viruses.

● Performed v iral i nfections w ith V accinia a nd H erpes S implex t o s creen f or a ntiviral a ctivity i n v ero c ells

(monkey k idney e pithelial c ells)

SKILLS A ND T ECHNIQUES

Molecular B iology T echniques

● Molecular C loning

● PCR a nd o ligonucleotide d esign

● Quantitative R eal t ime P CR

● Bacterial T ransformation

● Preparation o f e lectrocompetent a nd

chemically c ompetent c ells

Protein B iochemistry T echniques

● Cell c ulture o f m ammalian c ell l ines

● Expression a nd P urification o f p roteins

● SDS-PAGE, W estern B lotting, s ilver a nd

coomassie s taining

Animal W ork

● Bone m arrow m acrophage i solation f rom

mice

● In v ivo m ouse m odel: S almonella

Typhimurium i nfection ( IP i njection, O ral

gavage i nfection, c ardiac p uncture, t ail

bleeding, a nd o rgan r emoval)

Cellular I mmunology

● ELISA a nd f low c ytometry

● Histology/Microscopy: I mmunofluorescence

staining, f luorescence m icroscopy, a nd

confocal m icroscopy.

● Viral i nfections i n m onkey k idney e pithelial cells

Instrumentation

● Fast P rotein L iquid C hromatography ( FPLC) using A KTA p ure

Software:

● Microsoft W ord, E xcel, P owerPoint,

GraphPad P rism V ersion 5 .0c, I mageJ

● BD C ellQuest P ro V ersion 6 .0, F ACSDiva, FLowJo V ersion 8 .8.6,

● Adobe p hotoshop, A dobe i llustrator

● Serial C loner

PUBLICATIONS

Tursi S A, L ee E Y, M edeiros N J, e t a l. B acterial a myloid c urli a cts a s a c arrier f or D NA t o e licit a n autoimmune r esponse v ia T LR2 a nd T LR9. C oombes B K, e d. P LoS P athogens. 2 017;13(4):e1006315. doi:10.1371/journal.ppat.1006315.

Medeiros N J, T ursi S A, L ee M H, G allucci S, W ilson R P, T ükel Ç . O ral i nfection o f m ice w ith S t yphimurium leads t o t he g eneration o f a nti-dsDNA a utoantibodies v ia t he e xpression o f c urli. M anuscript i n p reparation 2

N. J . M edeiros, M .S.

POSTER P RESENTATIONS

2015-Eastern P ennsylvania B ranch o f A merican S ociety f or M icrobiology 2 4t h a nnual I nfection a nd I mmunity Forum, P hiladelphia, P A

2015-American S ociety f or M icrobiology 7 t h A SM C onference o n B iofilms, C hicago, I L PROFESSIONAL M EETINGS/SYMPOSIUMS

2017-Neuroepigenetics S ymposium, S milow C enter f or T ranslational R esearch, P erleman S chool o f M edicine, University o f P ennsylvania, P hiladelphia, P A 2017-Eastern P ennsylvania B ranch o f A merican S ociety f or M icrobiology 7 51st m onthly m eeting, T homas Jefferson M edical S chool, P hiladelphia, P A 2016-Eastern P ennsylvania B ranch o f A merican S ociety f or M icrobiology 7 44th a nd 7 45th m onthly m eetings, Thomas J efferson M edical S chool, P hiladelphia, P A 2015- E astern P ennsylvania B ranch o f A merican S ociety f or M icrobiology 2 4t h a nnual I nfection a nd I mmunity Forum, P hiladelphia, P A

2015- A merican S ociety f or M icrobiology 7 t h A SM C onference o n B iofilms, C hicago, I L 2015- M orton K lein S tudent C onference, D epartment o f M icrobiology a nd I mmunology, T emple U niversity School o f M edicine, P hiladelphia, P A

HONORS, A WARDS, A ND S PECIAL R ECOGNITIONS

2015- A merican S ociety f or M icrobiology 7 t h A SM C onference o n B iofilms T ravel G rant, C hicago, I L 2012-2014 W est C hester U niversity H onors C ollege S eminar C ertificate P rogram- m aintaining a m inimum 3 .25 GPA, b eing a ctively i nvolved i n o n-campus o rganizations a nd a ctivities a nd t aking f our H onors s eminar c ourses throughout t he r emainder o f t he u ndergraduate d egree. C ertificate o f M erit a warded u pon c ompletion. Fall 2 010, F all 2 011-Spring 2 014 D ean’s L ist; C ollege o f H ealth S ciences- A chieved a c umulative g rade p oint average o f a 3 .75 o r h igher.

LEADERSHIP

2017 S arma L ab S tudent W ork S tudy M entor- m entored a U niversity o f P ennsylvania w ork s tudy s tudent b y providing g uidance a nd h ands-on i nstruction o f v arious b iochemical a ssays. 2014 ( Spring) P hi E psilon K appa T reasurer- W ork w ith m embers a nd F raternity c hapter t o s ubmit p ayments for m embership, a s w ell a s m aintain m oney a ccrued t hrough f undraisers a nd F raternity-related s ales. 2012-2014 H onors S eminar C ertificate P rogram R ecruitment C ommittee C hair- Help r ecruit i nterested academically i nclined s tudents t o j oin t he H onors S eminar C ertificate P rogram b y r eaching o ut t o W est C hester University s tudents. W orked w ith e xecutive b oard t o c reate a h ooding c eremony f or g raduating s eniors. SERVICE A ND O UTREACH

2017 P hiladelphia S cience F estival V olunteer- V olunteered f or t he S cience F estival f acilitated b y t he F ranklin I nstitute, used t o e ncourage e ngagement i n S TEM f ields. V olunteers e xplain s cientific c oncepts a nd h elp f estival a ttendants participate i n f un, e ngaging a ctivities, h elping p romote t he c onversation o f s cience t o a w ide r ange o f a ge g roups. 3

N. J . M edeiros, M .S.

References a vailable u pon r equest

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N. J . M edeiros, M .S.

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