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Research Scientist

Location:
Atlanta, GA, 30312
Posted:
March 16, 2018

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Resume:

KRISTEN M. LAMB, PH.D.

Phone: 404-***-**** (Cell)

E-mail: ac4t3y@r.postjobfree.com

KM Lamb CV – page 1 of 7

OBJECTIVE

Utilize my skills and experience for the advancement of biological research in human and/or animal health at a high-impact company.

TECHNICAL SKILL SET

Biochemistry Experience:

Protease activity assay (using Folin-Ciocalteau reagent), electrophoresis, SDS PAGE, Western Blotting, immunofluorescence assays, protein folding/unfolding, circular dichroism (CD), dynamic light scattering (DLS), enzyme activity and inhibition assay and IC50 determination (spectrophometric). Modified activity assays for given proteins (procedures are used to this day in that lab). Executed, modified, and produced protocol for non-specific protease activity of unknown protease from organism. Protein Expression and Purification:

Construction of expression plasmids, transformation of E. coli with plasmids, and protein expression and purification (cation exchange, reverse phase (RP), size exclusion (SEC), affinity chromatography

(nickel, cobalt, methotrexate) using AKTA FPLC, peristaltic pump, gravity flow, and other FPLC/HPLC systems). Protein expression and construct designing also includes membrane-associated proteins. Expressed proteins in E. coli expression systems as well as in mammalian cells. Produced from scratch protocols for protein purification for given proteins (some novel) and modified purification procedures based on construct variation.

Protein Characterization:

Protein concentration (Bradford and Lowry protein assays), Circular dichroism (CD), dynamic light scattering (DLS), active site analysis utilizing online programs (ex: DoGSiteScorer), protein folding prediction programs (ex: Phyre2 Server), Sybyl small molecule docking. Molecular Biology Experience:

Plasmid and genomic DNA purification (cell culture, clinical tissue samples, E. coli, blood, feces), RNA purification (cell culture, from purified virus, clinical tissue samples), UV/Vis determination of DNA quantity and purity, PCR, qPCR, reverse transcription PCR, cloning, site directed mutagenesis, electrophoresis of DNA and RNA, DNA sequence analysis, DNA restriction digests, homologous recombination, oligonucleotide designs for PCR-based assays. Identified presence or absence of DNA sequence within cDNA from isolated RNA from infected cell culture. Design/analysis tools used – Serial Cloner, Sequencher, Vector NTI, NCBI website tools (ex: BLAST). Cell Culture Experience:

Cell transfection, stable cell line formation. Cell lines: BHK, BHK-T7, Vero, Vero-hSLAM, Hep2, MDCK, Fibroblast, CRFK.

KRISTEN M. LAMB

KM Lamb CV – page 2 of 7

Structural Biology Experience: Crystallography and Microscopy– Successfully crystallized a number of ternary structures of DHFR (Klebsiella pneumoniae, Vancomycin-resistant enterococcus, human), moved from cloning and protein purification to crystal production, freezing, X-ray crystallography data collection, scaling, refinement, and analysis for structure-based drug. Experience with PDB structure submission. Used these structures and others for small molecule docking into crystal structures using Sybyl.

Crystallography tools used – HKL2000, d*Trek, CCP4, Phenix, Coot, Pymol, MolProbity, Chimera, RCSB PDB Validation tools.

Electron Microscopy – cryo-tomography sample preparation (included but not limited to growth of cells infected with Measles virus on cryo-EM grids and then freezing them), cryo-tomography data reconstruction and analysis, negative stain EM, immune-labeled EM, thin-sectioning EM. Electron Microscopy tools used – IMOD, Amira.

Virology Experience:

Viral titration, viral propagation, purification and characterization, serum neutralization (SN) assays

(fluorescence and syncytia-based detection methods), production of viral vector constructs, RNA Dependent RNA polymerase (RDRP) assay (luminescence-based assay, related to MOA of small molecule), viral plaque assay, proof-of-concept work for modified SN assays with fluorescently labeled virus, cell based assays for small molecule anti-viral capacity. FeLV ELISA assay execution for project team.

Viruses: Hepatitis C, Venezuelan equine encephalitis virus (VEEV), Respiratory Syncytial Virus (RSV), Measles Virus (MeV), Canine distemper virus (CDV), Canine Parvovirus (CPV), Canine adenovirus type 2 (CAV2), Canine parainfluenza virus (CPi).

Chemistry:

Column chromatography, extractions, distillations, experience in graduate level organic synthesis lab in the pharmacology department at UTMB

Other Related Experience and Qualification: BSL2/BSL3 environments, clearance for working on select agent (VEEV), scientific publication writing, laboratory protocol writing, experience in training others in laboratory techniques, experience leading junior scientists (2 years in depth), experience in X- ray facility management and training of individuals in data collection and initial data processing techniques.

EXPERIENCE

Liberty Biosecurity- Worcester, Massachusetts 2017-2017 Research and Development

Research Scientist

Independently ran and drove two biochemical efforts of the start-up company. The first was executing protease assays routinely on a novel protein. Part of this entailed working to determine divalent cation dependency of the unknown protease. The second front was protein purification and purification optimization of two protein related to a microbiological assays which served as an integral part of KRISTEN M. LAMB

KM Lamb CV – page 3 of 7

another branch of the company. This being said, I worked closely with microbiology arm of company to provide protein reagents at quality and quantity required under intense time lines. The purification procedures I modified, and in one case made from scratch, are standard for this laboratory to date. Merial, a Sanofi Company (Contract)- Athens, Georgia 2016-2016 Biological Research and Development

Scientist II

Executed and optimized serum neutralization assays. Carried out ELISA assays. Worked towards sequencing in-house parvovirus strain and designing a fluorescently labeled strained thereof for the purposes of designing a more cost-efficient cell culture assay. Headed up efforts for designing method for more cost-efficient parainfluenza serum neutralization assay. Provided data for and interacted with project team members for multiple projects and did so under deadlines and . Routinely isolated DNA/RNA from clinical samples including blood, cartilage, and tendon etc.) Ran qPCR and reverse transcription PCRs. Cloned full-length parvovirus into larger vector. Routinely produced formal assay results reports for multiple project teams and maintained database of results in Analytical Database and in-house reagents in Freezer Works system. Designed oligos for PCR-based assays. Georgia State University – Atlanta, Georgia 2015-2016 Center for Inflammation, Immunity, and Infection

Postdoctoral Associate

Manipulated and generated of full-length respiratory syncytial virus and canine distemper virus. Performed mutagenesis of fusion proteins of respiratory syncytial virus for generating a full-length recombinant virus. Rescued recombinant viruses in cell culture. Designed full-length recombinant respiratory syncytial virus strains. Used RDRP assays to aid in determination of mechanism of small molecule inhibition of viral infection. Carried out compound dose response assays in mammalian cells. Performed TCID50 assays for Measles and respiratory syncytial virus titering (using fluorescence, luminescence, or syncytia depending on the recombinant strain). Maintained and aided efforts for improvement of in-house database for reagents. Isolated RNA/DNA from infected mammalian cells for RT-PCR, and PCR analysis of infected stain. Routinely ran MIDI and MINI prep purifications of plasmids.

Emory University – Atlanta, Georgia 2014-2015

Department of Pediatrics, Division of Infectious Diseases Postdoctoral fellow

Conducted In vitro assembly of respiratory syncytial virus matrix protein lattice. Aimed to determine arrangement of Measles Virus (MeV) glycoproteins in mature virions. Performed cryo-tomography reconstructions of RSV-infected cells to understand protein arrangements and changes at early-stages of infection. Purified respiratory syncytial virus matrix protein from E. coli protein expression system and produced liposomes from Avanti lipid to understand protein behavior within context of viral membrane environment. Designed and executed reactions for respiratory syncytial virus matrix lattice assembly. Evaluated assembly reactions by negative staining and viewing on JEOL1400 microscope. Grew HeLa cells on quantifoil grids and infected them successfully with Measles virus strains Measles-HcHisTagFlag and Measles-GFP. Evaluated infection of HeLa cells with Measles-GFP virus via fluorescence. Cryo-plunged froze infected HeLa cells on grids for cryo- KRISTEN M. LAMB

KM Lamb CV – page 4 of 7

tomography data collection. Processed cryotomography data resulting from above mentioned Measles grids as well as from other projects in the lab using IMOD software. Processed cryo-tomography data to support projects of other laboratory members. Carried out TCID50 assays for Measles tittering. In this position, also contributed heavily to other projects beyond the ones I headed up when projects were under tight deadlines.

University of Connecticut – Storrs, Connecticut 2011-2014 Department of Pharmacy

Postdoctoral fellow

Performed X-ray Crystallography of DHFR from three species. Expressed and purified Vancomycin- resistant enterococcus DHFR, human, and Klebsiella DHFR. Produced crystals of all three varieties. Produced multiple high-resolution diffraction X-ray crystallization data sets. Analyzed ternary crystal structures of DHFR from three species for the purposes of understanding structural variations between these and other species to guide designs of inhibitors for each. This includes characterizing and analyzing molecular differences between these. Performed Sybyl small-molecule docking as well as active site binding characterization using online tools for the purposes of understanding molecular binding site of proteins and biophysical characteristics affecting inhibitor binding. Successfully completed first structure of Klebsiella pneumonaie DHFR (ternary structure with cofactor and inhibitor). Performed IC50 assays with human, Vancomycin-resistant enterococcus, and Klebsiella pneumoniae DHFR inhibitors to aid in further inhibitor designs. This is for the purpose of understanding the activity of the proteins, and how they vary under the influence of small molecules with changes in side-chain or ring design. Managed and trained persons on the in-house X-ray beamline. Instructed, demonstrated, and advised two students’ biochemical and X-ray crystallography techniques for PharmD student projects. Served as guest lecturer for course, “Structure and Function of Biological Macromolecules.” As guest lecturer, described general X-ray crystallography procedures, beginning at setting up crystallization trials. Reviewed X-ray facility instrumentation and initial computational methods for solving structures. Served as assistant to ECE Professors’ Training Courses (2 sessions). During this, I reviewed practical methods in X-ray crystallography.

University of Texas Medical Branch – Galveston, Texas 2003-2010 Department of Biochemistry and Molecular Biology

Graduate Research

Produced cryo-EM single-particle reconstructions of pre- and post-entry nucleocapsids. Conducted pseudo-atomic modeling with crystal structure of capsid protein with a series of Venezuelan equine encephalitis virus mature nucleocapsid cryo-EM reconstructions at decreasing resolutions. Investigated Hepatitis C core protein construct’s ability to fold and participate in nucleocapsid-like-particle assembly. Expressed several protein constructs of Hepatitis C virus core protein. Sought to form uniform, homogeneous nucleocapsid-like-particles of Hepatitis C for structural studies, including cryo-EM. Used negative staining to evaluate Hepatitis C nucleocapsid-like-particles. KRISTEN M. LAMB

KM Lamb CV – page 5 of 7

University of West Georgia – Carrollton, Georgia 2000-2003 Department of Chemistry

Undergraduate Research/Organic Laboratory TA/General Chemistry Workshop Leader (Formal group study session leader)

Performed extractions on Cunninghamella elegans with nitro-polyaromatic hydrocarbons (nitro-PAHs) to evaluate whether the fungus could successfully biodegraded the nitro-PAHs. Made reagents for lab, helped students perform experiments, and further explained the chemistry to students. Lead discussions and problem-solving session in general chemistry.

COMPUTER EXPERTISE

• Linux, Macintosh, and Windows Platforms.

• MS Office (Word, Excel, Access, PowerPoint).

• Sybyl for small molecule docking into protein structures

• Phenix, CCP4, Coot, d*Trek, HKL2000 for crystallography

• Adobe PhotoShop, Acrobat, Image J, Quicktime.

• Having managed and maintained an R-Axis IV in-house crystallography beamline, I am quick to familiarize myself with new instrumentation. This is also due to spending years in graduate school working on JEOL 2100 cryo-EM microscope.

EXTERNAL PUBLICATIONS

Lamb, Kristen, et al. Antimicrobial Agents and Chemotherapy. 2014 December; 58(12): 7484-7491.

“Novel structure of Klebsiella pneumoniae dihydrofolate reductase and implications for inhibition.” Lamb, K.M., et al., Biochemistry. 2013 October 15; 52(41): 7318-26. “Elucidating features that drive the design of selective antifolates using crystal structures of human dihydrofolate reductase.” Lamb, Kristen, et al., Virology. 2010 October 25; 406(2): 261-9. “Structure of a Venezuelan equine encephalitis virus assembly intermediate isolated from infected cells.” Lamb, Kristen, et al., Virology. 2010 October 25; 406(2): 261-9. “Structure of a Venezuelan equine encephalitis virus assembly intermediate isolated from infected cells.” G-Dayanandan, N., Paulsen, J.L., Viswanathan, K., Keshipeddy, S., Lombardo, M.N., Zhou, W., Lamb, K.M., Sochia, A.E., Alverson, J.B., Priestly, N.D., Wright, D.L., Anderson, A.C., J. Med. Chem. 2014 February 25; 57(6): 2643-2656. “Propargyl-linked antifolates are dual inhibitors of Candida albicans and Candida glabrata.”

Yan, D., Weisshaar, M., Lamb, K., Chung, H.K., Lin, M.Z., Plemper, R.K., Biochemistry. 2015 September 15; 54(36): 5589-5604. “Replication-Competent Influenza Virus and Respiratory Syncytial Virus Luciferase Reporter Strains Engineered for Co-infections Identify Antiviral Compounds in Combination Screens.”

KRISTEN M. LAMB

KM Lamb CV – page 6 of 7

Yi, H., Strauss, J.D., Ke, Z., Alonas, E., Dillard, R.S., Hampton, C.M., Lamb, K.M., Hammonds, J.E., Santangelo, P.J., Spearman, P.W., Wright, E.R., J. Histochem. Cytochem. 2015 October; 63(10): 780- 792. “Native Immunogold Labeling of Cell Surface Proteins and Viral Glycoproteins for Cryo-Electron Microscopy and Cryo-Electron Tomography Applications.” INTERNAL PRESENTATIONS

Oral Presentation: Towards a More Cost-efficient Canine Parainfluenza (CPi) Virus Serum Neutralization (SN) Assay. B&B Merial Meeting. December 15, 2016. Oral Presentation: Determining Glycoprotein Arrangement and Assembly Details in Measles Virus. Emory Children’s Center: Division of Infectious Disease Monthly Meeting. December 18, 2014. EXTERNAL PRESENTATIONS

Poster Presentation: Elucidating Features that Drive the Design of Selective Antifolates Using Human Dihydrofolate Reductase. 44th Mid-Atlantic Macromolecular Crystallography Meeting in Durham, North Carolina, May 2013.

Poster Presentation: Elucidating Features that Drive the Design of Selective Antifolates Using Human Dihydrofolate Reductase. North Eastern Structure Symposium (NESS) in Hartford, Connecticut, May 2013.

Poster Presentation: Understanding Molecular Interactions Regulating Venezuelan Equine Encephalitis Virus Nucleocapsid Plasticity. Keck Annual Research Conference in Houston, Texas, November 2007. Poster Presentation: (multiple years) Understanding Molecular Interactions Regulating Venezuelan Equine Encephalitis Virus Nucleocapsid Plasticity. Sealy Center for Structural Biology Symposium. May 2006-2010.

EDUCATION

Ph.D.-Biomedical Sciences (Biochemistry and Molecular Biology) University of Texas Medical Branch, Fall 2010.

Dissertation: Understanding the Assembly of Simple ssRNA Virus Nucleocapsids. Impact: Increasing the understanding of early stage and assembly of mature virions could increase the opportunities for therapeutics exploiting these points. B.S.-Chemistry, University of West Georgia, Spring, 2003 KRISTEN M. LAMB

KM Lamb CV – page 7 of 7

HONORS

Keck Fellowship Recipient W. M. Keck Center for Virus Imaging 2005-2007 Pharmacology Scholarship UTMB Pharmacology Department 2003-2004 AFFILIATIONS

Biophysical Society, 2011 to 2017.

American Chemical Society, 2001 to 2010.

REFERENCES

1. Bradley Feilmeier, M.S.

Manager, Bioanalytical Clinical Group (DIFA), Boehringer-Ingelheim ac4t3y@r.postjobfree.com

706-***-****

2. Rene E. Vasquez, Ph.D.

Medical Science Liaison, Shire Pharmaceuticals

ac4t3y@r.postjobfree.com

210-***-****

3. Karen Lavery, Ph.D.

Sr. Manager, Metro Biotech

ac4t3y@r.postjobfree.com

508-***-****

4. Bruce Szczepankiewicz, Ph.D.

(Character Reference)

Director of Development Chemistry, Catabasis Pharmaceuticals https://www.linkedin.com/in/bruce-szczepankiewicz-39218b8/



Contact this candidate