Manager High School

Resume

Joseph Anthony Pope Jr.

805-***-****, *******@***.***

Born January 25, 1953

Education

Grades 1-8: St. Michael’s School, Cranford NJ

High School Cranford High School, Cranford NJ, 1968-1970 Graduated Cinnaminson High School, Cinnaminson NJ

1971

College: University of Miami, Coral Gables, FL 1971-1974 Graduate School:

Rutgers University: Ph.D. in Microbiology, 1985

Employment:

1974-1977: Lab Technician, Waksman Institute of Microbiology Fermentation Pilot Plant

1977-1981: Manager: Large Scale Fermentation Pilot Plant, Waksman Institute of Microbiology, Rutgers University, Conducting lab courses in large scale fermentation, Maintained equipment including 2 1000 L fermentors, Filter Press, Homogenizer, Sharples Centrifuges, large-scale lyophilizer: Produced some of the original batches of cell paste (H. influenza sp.) used for the production of Restriction Endonucleases for Bethesda Research Labs) for production of various enzymes, Produced cell paste containing antibiotics from a variety of species (Streptomyces sp, Micromonospora sp)

1986-2005: Fermentation Microbiologist and Analytical Scientist at Amgen Inc., Thousand Oaks. Conducted fermentations for the production of a variety of recombinant products using bacterial cultures (E. coli, B. subtitlis) and yeast (S. cerivisiae, P. pastoris)

Conducted HPLC quantitation of fermentation products. Notable Contributions to Amgen technology:

Use of automated time feeding protocols to produce high density cultures of microorganisms

Implementation of cheaper, food-grade raw material, including food-grade glucose and food-grade yeast extract in place of higher priced reagent grades Use of Statistical Process Optimization Techniques to optimize yield of a novel product fermentation

Implementation of HPLC as fermentation product quantitation method to replace less reproducible SDS-PAGE analysis used previously. Use of Statistical Process Control methods to increase confidence in HPLC quantitative results

Introduction, development and improvement of Pichia pastoris technology to fermentation lab

Replacement of SDS-PAGe quantitation of product concentration with more reproducible HPLC quantitation.

Participated in writing and reviewing several SOP’s and Analytical Methods Participated in writing, reviewing and filing 2 IND’s for new products Retired 2005

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