Resume

Sign in

State University Plant

Location:
Raleigh, North Carolina, 27603, United States
Salary:
55000
Posted:
November 13, 2017

Contact this candidate

STEVEN BOWEN

ac3aqz@r.postjobfree.com

(919) ***-****

**** ***** *** ***

Raleigh, NC 27603

OBJECTIVE

To find a position that allows me to aid in scientific discoveries relating to research in the fields of biotechnology, pharmaceuticals, microbiology, and/or agronomy.

EXPERIENCE

Research Associate – Department of Crop Science, North Carolina State University, Raleigh, NC

Feb 2002- Dec 2014

Applied the techniques of molecular biology toward identification and characterization of genes in tobacco to introduce harm reduction traits, as well as other genes of potential agronomic value.

Duties and job responsibilities included the generation and management of large data sets such as sequencing and micro-array data, development and propagation of transgenic tobacco plants, alkaloid analysis, molecular marker assays for the screening of large sets of tobacco plants possessing targeted gene modifications, and the care and cultivation of tobacco plants in the laboratory, greenhouse, and field.

Skills acquired beforehand and further developed, and including new ones, consisted of PCR, Southern, Northern, and Western analysis, Sanger and next generation sequencing, development and use of DNA molecular markers, genotyping, epigenetic analysis, and gene expression studies.

A primary objective was achieved in the ongoing efforts relating to harm reduction in tobacco use. The functional development of a non-GM approach through mutational breeding of tobacco to introduce stable knockout mutations in three key genes that encode nicotine N-demethylase activity in select cultivars was accomplished. Such plants were shown to have a dramatic reduction in one of the known tobacco-specific nitrosamines, which are recognized as potent carcinogens.

Postdoctoral Research Fellow – Department of Food, Bioprocessing, and Nutrition, North Carolina State University, Raleigh, NC

July 2001- Jan 2002

Research relating to the study of virulence, molecular epidemiology, and genetics of the foodborne pathogen, Listeria monocytogenes.

Research Associate – Department of Microbiology, North Carolina State University, Raleigh, NC

Nov 1997- June 2001

Investigated the biosynthesis and biological roles of Superoxide Dismutases (SODs) and Hydroperoxidases, key enzymes involved in combating cellular oxidative stress. Primary focus was research on gene regulation of SODs and Oxidative Stress Regulons in Escherichia coli and Streptococcus thermophilus.

Postdoctoral Research Fellow – Department of Infectious Disease and Microbiology, University of Pittsburgh, Pittsburgh, PA

Aug 1994- Feb 1997

Employed antisense RNA technology to inhibit Hepatitis B virus gene expression in cell culture by gene transfer as an approach to block immune recognition and pathogenic sequelae. The retroviral-mediated antisense gene transfer combined with liver (or hepatocyte) transplant could provide a molecular targeting approach for treating chronic hepatitis patients.

Postdoctoral Research Fellow – Department of Plant Molecular Biology, Tsukuba Science City, Japan

Feb 1993- Feb 1994

Exploited the natural plant DNA virus, Geminivirus, as proof of concept for use in various plant genetic manipulations such as gene targeting.

EDUCATION

Doctor of Philosophy – PhD (1993) Department of Microbiology, North Carolina State University, Raleigh, NC

Master of Science – MS (1988) Department of Food Science, North Carolina State University, Raleigh, NC

Bachelor of Science – BS (1983) Food Science, North Carolina State University, Raleigh, NC

Associate's degree – (1981) Brevard College, Brevard, NC

SELECT SKILLS

Basic molecular biology techniques

Protein extraction, isolation, and characterization

Molecular cloning

Gene expression studies including protein and gene fusions

Genetic transfection and transformation techniques in mammalian, plant, and bacterial cells

Tissue culture techniques

Flow cytometry

RNAi technology

Acquisition and management of large biological data sets

Antigen assays (ELISA)

Generation of transgenic plants

Use of next generation machinery for DNA manipulations and analysis

Generation and development of various DNA molecular markers

Traditional, high-throughput, and next generation sequencing

Familiar with various enzymatic assays

PUBLICATIONS

Lewis RS, Lopez HO, Bowen SW, Andres KR, Steede WT, and Dewey RE. (2015) Transgenic and mutation-based suppression of berberine bridge enzyme-like (BBL) gene family reduces alkaloid content in field-grown tobacco. PloS One; 10(2):e0117273.

Li D, Lewis RS, Jack AM, Dewey RE, Bowen SW, and Miller RD. (2012) Development of CAPS and dCAPS markers for CYP82E4, CYP82E5v2, and CYP82E10 gene mutants reducing nicotine to nornicotine conversion in tobacco. Mol. Breeding 29(3):589-599.

Lewis RS, Bowen SW, Keogh MR, and Dewey RE. (2010). Three nicotine demethylase genes mediate nornicotine biosynthesis in Nicotiana tobacum L.: functional characterization of the CYP82E10 gene. Phytochemistry 71(17-18):1988-1998.

Chakrabarti M, Bowen SW, Coleman NP, Meekins KM, Dewey RE, and Siminszky B. (2008) CYP82E4-mediated nicotine to nornicotine conversion in tobacco is regulated by a senescence-specific signaling pathway. Plant Mol. Biol. 66(4):415-427.

Gavilano LB, Coleman NP, Bowen SW, and Siminszky B. (2007) Functional analysis of nicotine demethylase genes reveals insights into the evolution of modern tobacco. J. Biol. Chem. 282(1):249-256.

Siminszky B, Gavilano LB, Chakrabarti M, Dewey RE, and Bowen SW. (2007) Evolution of nicotine N-demethylase genes and their use in reducing nornicotine levels in tobacco. Recent Adv. Tob. Sci. 33:27-38.

Siminszky B, Gavilano LB, Bowen SW, and Dewey RE. (2005) Conversion of nicotine to nornicotine in Nicotiana tobacum is mediated by CYP82E4, a cytochrome P450 monooxygenase. Proc. Natl. Acad. Sci. USA. 102(41):14919-14924.

Andrus JM, Bowen SW, Klaenhammer TR, and Hassan HM. (2003) Molecular characterization and functional analysis of the manganese-containing superoxide dismutase gene (sodA) from Streptococcus thermophilus A054. Arch. Biochem. Biophys. 420(1):103-113.

Tung FY, Bowen SW. (1998) Targeted inhibition of hepatitis B virus gene expression: a gene therapy approach. Front. Biosci. 3:a11-5.

Bowen SW, and Hassan HM. (1993) Characterization of cis-acting regulatory mutations causing anaerobic expression of the sodA gene in Escherichia coli. Arch. Biochem. Biophys. 302(2):372-379.

Bowen SW, and Hassan HM. (1988) Induction of manganese-containing superoxide dismutase in Escherichia coli is independent of the oxidative stress (oxyR-controlled) regulon. J. Biol. Chem. 263(29):14808-14811.

U.S. PATENTS ISSUED

U.S. Patent 9,228,195 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Jan. 5, 2016) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.

U.S. Patent 9,228,194 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Jan. 5, 2016) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.

U.S. Patent 9,187,759 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Nov. 17, 2015) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.

U.S. Patent 8,124,851 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Feb. 28, 2012) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.

U.S. Patent 7,884,263 “Alteration of Tobacco Alkaloid Content through Modification of Specific Cytochrome P450 Genes” (Feb. 8, 2011) Inventors: R.E. Dewey, B. Siminszky, S.W. Bowen and L. Gavilano.

The above patents show the compositions and methods for reducing the level of nornicotine and N'-nitrosonornicotine (NNN) in Nicotiana plants. Methods find use in production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

REFERENCES (available upon request)



Contact this candidate