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Bioenginnering

Location:
Philadelphia, Pennsylvania, United States
Posted:
November 13, 2017

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Xinglong Shi

215-***-**** ** Hawkswell Cir. Oreland, PA 19075 ac3aos@r.postjobfree.com

SUMMARY: Master graduate with strong academic background in Bioengineering and extensive and diverse technical skills such as tissue cell culture, molecular, cell-based and immunological assay methods, protein chemistry and nucleic acid techniques. Highly motivated, productive, logical and known as a good team player.

TECHNICAL SKILLS:

Cell Culture: Basic cell culture (Mammalian Cells and Neural Stem Cells), immunocytochemistry, cytotoxicity assay, cell migration assay, and transfection.

Protein& Nucleic Acid Techniques: DNA& RNA isolation, PCR, Reverse Transcriptase PCR, Real-Time PCR, Protein extraction, Western blot, Synthesis of alginate and methacrylated hyaluronic acid hydrogel. Solid phase peptide synthesis. Rheology testing,

Animal Experience: Rat genotyping, injection, tissue embedding

Analytical and Photograph: HPLC,UV-Vis spectrometer, electrophoresis, 2-Dimensional Electrophoresis, confocal laser microscope, fluorescence microscope (Olympus IX-83).

WRITING & COMPUTER SKILLS:

Proficient with MS Office applications (Word, Excel, PowerPoint), Chemdraw, JMP. Efficient user of public databases such asPubmed, NCBI, ATCC, etc. Understanding of FDA regulatory of medical products.

PROFESSIONAL WORK EXPERIENCE:

Thomas Jefferson University, Philadelphia, PA

Research Technician A, June 2016-Present

Experienced with rats experiment including breeding, clipping, genotyping, injection.

Conducted rat tissue removal, fixation and embedding.

Cultured mammalian cells using sterile techniques. Performed, cryopreservation, expansion of cell seed stocks, growth of cells in preparation of experiments and harvest of cells for downstream applications. DNA and miRNA cell transfection

Performed western blot, immune-precipitation, 2-Dimensional Electrophoresis for tissue and cell samples.

Constructed recombinant DNA in E.Coli from restriction digest of plasmids to plasmid mini & maxi prep, DNA sequencing.

Conducted DNA and RNA isolation, PCR, qPCR, gel electrophoresis.

Designed and implemented molecular based experiment according to PI’s requirements.

Assisted in establishment of a basic research laboratory including work area allocations, initial equipment and supply purchases. Managed lab space, supplies, equipment maintenance and laboratory safety compliance. Received, verified and kept record of new deliveries. Prepared routine reagents for daily experiment.

Developed and validated new experimental SOPs for newly-purchased equipment and reagents.

Trained new staffs, visit scholars and student interns.

Dr. Suh Lab in Temple University-Bioengineering, Philadelphia, PA

Master student, April 2014-Dec. 2015

Developed and improved alginate and hyaluronic acid based hydrogel for 3D cell culture system.

Generated and characterized peptide amphiphiles by solid phase peptide synthesis, HPLC and Mass-spec.

Achieved experimental thesis research studying “ neural stem cell differentiation experiment in 2D and 3D culture system using small molecules”. Techniques included cell culture, spectrometer, cytotoxicity assay (LDH, Presto Blue, and WST-8), RNA isolation, RT-qPCR, Immunocytochemistry.

Developed and Validated new reagents and equipment in the lab, wrote SOPs for the lab.

Presented poster and thesis to faculty and students

Guided new lab members (undergraduates and masters).

Xiaoli Zhao’s Lab in Zhejiang University-College of Life Science, Hangzhou, China

Senior Research member, May 2011 – April 2012

Performed basic experimental research studying human embryonic stem cells differentiation to osteoblast using LiCl. Characterized the results using RT-qPCR and electrophoresis. Other responsibilities included keep record of cell maintenance and data analysis using Excel.

Zhejiang Dragon Chemical Group, Hangzhou, China

Lab Assistant, July 2012 – Sep 2012

Collection and organization of experiment results using Excel.

Arranged daily meeting. Received and shipped the package for the lab.

Updated daily data to the system. Searched and organized the articles.

EDUCATION

Master of Science in Bioengineering, Temple University, Philadelphia, Pennsylvania 2012-2015

Bachelor of Biotechnology, Zhejiang University City College, Hangzhou, China 2008-2012



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