Microsoft Office Manager
Resume:
Brian Gettler
Louisville, KY 40204
Phone:859-***-**** Email:********@*****.***
Bioengineer with a very strong biological background due to applied experience in biological laboratories. Experience is focused on bio-printing, electrospinning, cell culture, and supporting analytical methods. Works well both independently and collaboratively. Constantly expanding knowledge in career field.
EXPERIENCE
Cardiovascular Innovation Institute- University of Louisville, Louisville, Ky
Research Engineering Scientist August 2015-Present
Full-time research position with emphasis towards tissue engineering topics. Plans, executes, and analyzes projects both independently and collaboratively. Primary areas of responsibility include electrospinning, bioprinting, and instrumentation/data acquisition. Manages portions of the lab and engineering interns.
First author of an accepted publication in Tissue Engineering Part C and third author of a second submitted publication to the same journal
Electrospun cell scaffolds with combinations of polymers and bio-molecules
Evaluated electrospun scaffolds with electron microscopy (SEM), in vitro cell culture assays, and basic histology of murine in vivo samples
Bio-printed/additive manufactured 3D spheroid culture systems with alginate and collagen
oPresented collagen spheroid project results orally at IFATS San Diego 2016
oSpheroid manufacturing method published as first author in Tissue Engineering Part C
Constructed mock flow loops with instrumentation/data acquisition for vascular graft modeling/DAQ
Lab management responsibilities following departure of previous lab manager
oGrant budget organization and expense tracking
oSupply purchasing and expense tracking
University of Louisville, Louisville, Ky
Research Technologist II April 2015-August 2015
Full-time research position to isolate and characterize cardiac cell populations. Responsible for all associated cell culture.
Isolated cell populations through collagenase digestion and subsequently through adherence speed
Additional cell isolations performed using antibody directed magnetic sorting
Characterized cell populations by gene expression/surface markers using flow cytometry, immunofluorescent imaging, and RT-PCR
University of Louisville & Energy Delivery Solutions, Louisville, Ky & Jeffersonville, Indiana
Graduate Student/Research Engineer August 2013-December 2014
Masters of Engineering thesis to evaluate the efficacy of antioxidants used in a radio-protective role. Responsible for planning the project with thesis advisors then executing the plan and analyzing the results independently.
Optimized by size and morphology then manufactured lipid vesicle delivery system with antioxidants
Tested delivery system and antioxidants in vitro with fibroblasts for cytotoxicity and antioxidant capacity
Cardiovascular Innovation Institute- University of Louisville, Louisville, KY
Research Engineer/Research Associate January 2013- July 2013
Full-time co-op/intern Independently manufactured and evaluated cellular scaffolds for use in a clinical pilot trial.
Manufactured bio-absorbable cell scaffolds for fistula plugs via electrospinning
Performed in vitro and histological evaluations on scaffolds for cell seeding and infiltration
Helped prepare 1st human clinical pilot
Allylix (now Evolva), Lexington, KY
Research Associate May 2012- August 2012
Full-time co-op/intern Troubleshot/optimized GC based terpene extraction assay with secondary tasks of recombinant yeast mutant screenings. Additional tasks of laboratory cleaning and maintenance.
Troubleshot an assay for gas chromatography based micro-culture screening
Performed yeast/E. Coli DNA transformations/transfections
Performed micro-culture mutant screening and shake flask mutant screening
Alltech, Lexington, KY
Laboratory Technician August 2011-December 2011
Full-time co-op/intern Worked independently to manufacture and evaluate a bioremediation product with long term goal of EPA submission.
Manufactured bioremediation product for testing
Data analysis tests performed on product for EPA submission purposes with focus on product growth rates and target material degradation rates
Displayed results in an open presentation
EDUCATION
Masters of Science, Bioengineering, University of Louisville, Louisville, KY, 3.48 GPA, December 2014
Bachelors of Science, Bioengineering, University of Louisville, Louisville, KY, July 2013
TECHNICAL SKILLS
General Lab Skills: Cell Culture, 2D and 3D fluorescent staining/imaging/quantitation, cell sorting methods, basic histology, basic data acquisition and filtering, limited lab management, use of basic wet lab equipment
Specific Instrumentation: Bioprinting, 3D printing, electrospinning, flow cytometry, confocal microscopy, scanning electron microscopy, Cytation5 timelapse imaging, gas chromatography, Gould/Powerlab data acquisition systems
Computer Skills: Chart5, Graphpad Prism, Minitab, Labview, Matlab, Microsoft Office, Windows
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