Professional Experience Clinical Research

KARTICK PRAMANIK, Ph.D.

* *********** ********, *******, **-17033

***************@*****.***, 806-***-****

EDUCATION:

Jadavpur University, Kolkata, WB, India

Ph.D., in Pharmaceutical Sciences (Con: Pharmacology), 2009

Discovered small molecule, PITC-2, from tissue culture P. Indica utilizing chromatography.

Evaluated pharmacological and toxicological studies of PITC-2 in vitro & in vivo.

Designed & conducted pharmacokinetics study (PK/PD) of liposomal PITC-2/PITC-2 on rabbits.

Jadavpur University, Kolkata, WB, India

Mater of Pharmacy (M.S.), Pharmacology, 2006

Jadavpur University, Kolkata, WB, India

Bachelor of Pharmacy (B.S.), Pharmaceutical Sciences, 2003

PROFESSIONAL EXPERIENCE:

Penn State College of Medicine, Dept. of Pharmacology, Hershey, PA 11/2015 – 6/2017

Assistant Professor (non-tenure track)

Elucidated molecular basis of carcinogenesis in vitro cell culture and in vivo PK/PD tumor efficacy models in developing different small molecules against pancreatic and prostate cancer.

Developed the role of oxidant and anti-oxidant by regulating redox signaling in cancer progression, chemo-resistance and metastasis by using small molecules like pseudolaric acid B (PAB), capsaicin.

Discovered novel cell death mechanism of ISC-4 in p53 dependent pathway in prostate cancer.

Supervised and mentored other associates in developing their projects in laboratory.

Texas Tech University Health Sciences Center, Dept. of Biomedical Sci., Amarillo, TX 1/2015 – 11/2015

Research Scientist

Evaluated in vivo preventive efficacy of selenium against carcinogen-induced androgen-promoted prostate carcinogenesis in rats and determine mechanism in P53-P21 pathway.

Explored mechanism of metastasis (EMT) in xenograft and orthotopic models in prostate cancer. Design and validating cell-based assay, pathway biomarkers validation.

Developed molecular mechanism of senescence and autophagy in vitro & in vivo.

Mentored undergraduate and graduate students in developing their projects.

Penn State University, Dept. of Toxicology and Carcinogenesis State College, PA 2012 to 2014

Post-Doctoral Researcher

Discovered novel role of nuclear receptor Peroxisome Proliferator-Activated Receptor β in metastasis in lung, liver, and colon cancer while developing stable GFP tagged PPAR/cell line.

Found role of PPARβ ligands in UVB induced P53 mutant patches in skin cancer with immunochemistry.

Pinpointed mechanism of PPARβ in lung cancer prevention by cell cycle arrest.

Texas Tech University Health Sciences Center, Dept. of Biomedical Sci., Amarillo, TX 2009 to 2012

Post-Doctoral Researcher

Studied novel mechanism of cell death induced by capsaicin through mitochondria death pathways.

Discovered ASK1 (Kinase)/Trx complex dissociation is essential pathway for activation of P-ASK.

Isolated the role of PI3K/AKT/FOXO signaling on pancreatic cancer prevention by BITC.

Identified the novel role of CAPE in melanoma through AKT/XIAP pathway.

Boosted pancreatic cancer prevention by evaluating β-Catenin/TCF-1 complex signaling.

Validated all project in vitro results to in vivo using xenograft or orthotopic animal models.

Duckbill Drugs Pharmaceuticals Kolkata, WB, India 2003 to 2004

Production Chemist in liquid, tablet, capsule formulation

Planned, conducted and interpreted formulation and process development experiments for drugs.

Developed and analyzed test samples and reviewed the outcomes.

Designed and produced pharmaceutical products in the form of capsules, powders, and tablets.

Prepared and optimized various pharmaceutical dosage forms and drug delivery systems.

Wrote and maintained master formulas, manufacturing records, test reports and related protocols.

Experienced in cGMP and GLP processes, FDA guidelines and other government regulations

SCIENTIFIC SKILLS (PRECLINICAL & CLINICAL RESEARCH):

Leverages experience in vitro and in vivo model selection and evaluation, design of PK/PD and efficacy studies, drug combination strategies. Highly experienced in rodent models.

Highly experienced in cell culture with primary cell lines and/or ex-vivo tissue samples.

Expertise in various microscopy and other advanced imaging technique, mechanisms of action and resistance of therapeutic agents of interest.

Experienced to lead pharmacology efforts for a small molecule drug discovery program

Coordination of laboratory samples and compile data and clinical study.

Molecular Biology: Cytotoxicity Assays, Multi-Color Flow-Cytometry (FACS), Apoptosis, Cell Cycle, Mitochondrial Oxidation, Transient & Stable Transfections, Western Blotting (ECL), Plasmid Extraction, DNA, RNA Isolation, PCR, q-PCR, EMSA, ELISA, siRNA Knockdown, Co-Immunoprecipitation (IP), Luciferase/Reporter/CAM/Zymography/Metastasis & Angiogenesis Assay, Autophagy Assay, Senescence Assay, Enzyme Assays and immunoassay.

In Vivo Pharmacology: Design & Execution of in vivo studies, Pharmacokinetics (PK) Behavioral Pharmacology in mice, blood and tissue samples analysis for biomarkers and target molecules.

Toxicology: Toxicity Studies (acute, subacute, subchronic, and chronic) in rat and rabbit models.

Cancer Models: Xenograft, Orthotopic, Metastasis, Skin cancer, Genetically engineered Models

Oncogenic Signaling: P13K/AKT, DNA Damage Through P53/P21, Mitochondrial Death Signaling, Redox Signaling, ASK-1/Trx Signaling, P13K/AKT/FOXO Signaling, AKT/XIAP Pathway, mTOR Signaling, βcatenin /TCF-1 Signaling, JNK/FOXO/BIM, Stat-3 Signaling, EMT Signaling, Hedgehog, ER Stress-Autophagy Signaling, Anoikis, Senescence, Receptor Tyrosine Kinase Signaling.

Microscopy: Light, Fluorescent, & Confocal; for Immunofluorescences & Immunohistochemistry

Protein: Protein Expression, Purification via Affinity Chromatography, & Separation techniques

Microbiology: Bacteria Culture, Transformation, HEK293 & Bacterial Expression, Plasmid Extraction, Isolation & Differentiation of Primary Cells (Keratinocyte)

Histology: H&E Staining, Cryo-section (frozen tissues), Paraffin Embedded Section

Chromatography: Column/liquid/thin layer chromatography, Lyophilizer, HPLC/HPTLC, LC-MS/MS.

LEADERSHIP, MENTORSHIP, TECHNOLOGICAL, & TEAMWORK ABILITIES:

Catalyzes effective laboratory operations by managing assistance and associates, documenting and analyzing data, and preparing publications/presentations of key findings.

Employs exceptional attention-to-detail and written communication skills to isolate and record data.

Mentored graduates, undergraduates, Postdocs for their professional training.

Work in team with other colleagues to facilitates each other projects

Demonstrates proficiency with all MS Office applications & GraphPad Prism, technical software

Experienced in grant writing.

PATENTS:

1.K.C. Pramanik & T.K. Chatterjee: “Invention of new pharmacologically active constituent (PITC-2) from tissue cultured Pluchea indica (L.) less plant.” (Application Number: 1990/Kol/2008 – India)

2.K.C. Pramanik T.K. Chatterjee: “Invention of novel antileishmanial drug delivery preparations of PITC-2.” (Application Number: 2124/Kol/2008 – India)

SELECTED PUBLICATIONS:

1.Kartick C. Pramanik, Neel M. Fofaria, Parul Gupta, Alok Ranjan, Sung-Hoon Kim, Sanjay K. Srivastava. Inhibition of β-Catenin signaling suppresses pancreatic tumor growth by disrupting nuclear β-Catenin/TCF-1 complex: Critical role of STAT-3. Oncotarget 2015, 6(13): 11561–11574

2.Pramanik KC, Fofaria NM, Gupta P, Srivastava SK. CBP mediated FOXO-1 acetylation inhibits pancreatic tumors growth by targeting SirT-1. Molecular Cancer Therapeutics (MCT) 2014, 13(3):687-98.

3.Kartick C Pramanik, Shashi Kudugunti, Neel Fofaria, Majid Moridani, Sanjay K. Srivastava. Caffeic acid phenethyl ester suppresses melanoma tumor growth by inhibiting PI3K/AKT/XIAP pathway. Carcinogenesis 2013, 34(9):2061-70.

4.Reilly CA, Henion F, Bugni TS, Ethirajan M, Stockmann C, Pramanik KC, Srivastava SK, Yost GS. Reactive Intermediates Produced from the Metabolism of the Vanilloid Ring of Capsaicinoids by P450 Enzymes. Chem Res Toxicol. 2013, 26, 5566.

5.Kartick C. Pramanik, Sanjay K. Srivastava. Apoptosis signal regulating kinase 1-Trx complex dissociation by capsaicin causes pancreatic tumor growth suppression by inducing apoptosis. Antioxid Redox Signal (ARS). 2012. 15; 17(10):1417-32

6.Kartick C. Pramanik, Sanjay K. Srivastava. Role of mitochondrial electron transport chain complexes in capsaicin mediated oxidative stress leading to apoptosis in pancreatic cancer cells. PLoS One. 2011; 6(5):e20151, 1-16.

7.Srinivas Reddy Boreddy, Kartick C. Pramanik, Sanjay K. Srivastava. Pancreatic tumor suppression by benzyl isothiocyanate is associated with inhibition of PI3K/AKT/FOXO pathway. Clinical Cancer Research. 2011; 17(7):1784-1795.

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