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Research Scientist

Location:
Lansdale, Pennsylvania, United States
Posted:
August 30, 2017

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Randall J. Derstine, M.S.

*** ********* **** ac128c@r.postjobfree.com

Lansdale, PA 19446 (267)-***-****

Education

M.S., Molecular Biology and Genetics Degree Obtained: January 2014

University of Delaware- Newark, DE

Research Advisor: Calvin L. Keeler, PhD

Thesis Title: “Avian Toll-Like Receptor 7 (TLR7) and Interferon Regulatory Factor 7 (IRF7)

Function in the Pro-Inflammatory Response to Single-Stranded RNA”

B.S., Biochemistry Degree Obtained: May 2010

Elizabethtown College – Elizabethtown, PA

Research Advisor: Jane F. Cavender, PhD

Thesis Title: “SV40 Large T-Antigen is Capable of Transactivating Nucleophosmin B23”

Technical/Research Skills

Molecular Biology

Cellular Biology

Immunological-Based Assays

In-Vivo

Platelet Biology

Computational

PCR

Mammalian Tissue Culture

(Thawing + Reviving Cells, Liquid Nitrogen Freezing)

Western Blotting

Mouse Genotyping (Ear Tagging, Tail Clipping, DNA Isolation, Primer Design, Agarose Gel Electrophoresis)

Phlebotomy (Human)

Bioinformatics

qRT-PCR

Aseptic Technique

ELISA (MSD)

Mouse Immunization (IV, IP, Sub-Q)

Platelet Isolation/Washing

FlowJo

RNA-Interference

(siRNA, shRNA)

DNA Transfection (Calcium Phosphate, DEAE-D, Lipofectamine)

Immunofluorescence

Spinning Disc-Confocal Microscopy Cremaster Laser Ablation Model

Optical Platelet Aggregometry

GraphPad Prism

Bacterial Culturing/Plasmid Cloning + Isolation

Luciferase Assay

Flow Cytometry (BD Platform + FlowJo)

Antigen Preparation (Freund’s Adjuvant System, Protein Beads, Plasmid DNA Crystals)

Whole Blood Micro-Fluidic Thrombosis Assay

ImageJ

Bacterial Transformation

In-Vitro Kinase Assay

Co-ImmunoPrecipitation

Tissue Processing

Quantitative TxB2 ELISA Assay

B-DNA Multiplex PCR/Luminex Assay (Affymetrix)

Bradford Assay

Relevant Work Experience

1.Johnson & Johnson, Janssen Pharmaceuticals Discovery Immunology

a.Research Associate Scientist I

b.Supervisor: Michael S. Scully

c.May 2017-Present

d.Spring House, PA

e.Participating in early drug discovery research pipeline relating to Inflammatory Bowel Disease (IBD).

2.Thomas Jefferson University Department of Medicine

a.Research Associate Scientist I

b.August 2014-February 2017

c.Philadelphia, PA

d.Worked in the field of vascular biology studying the role of MAP3K signaling in human platelet activation.

3.University of Delaware Department of Biological Sciences

a.Graduate Teaching and Research Assistant

b.Supervisor: Calvin L. Keeler, PhD

c.January 2012-January 2014

d.Newark, DE

e.Performed research in the laboratory of Dr. Calvin L. Keeler, PhD elucidating the role of avian TLR7 and IRF7 in mediating the pro-inflammatory response to ssRNA.

f.Taught introductory biology to undergraduate students for four semesters.

4.Strategic Diagnostics, Inc. (SDIX, Inc.)

a.Research Scientist I

b.Supervisor: Dale V. Onisk, PhD

c.June 2010-May 2011

d.Newark, DE

e.Worked in Research & Development in the field of hybridoma technology developing monoclonal antibodies.

5.United States Army Medical Research Institute of Infectious Diseases (USAMRIID)

a.Research Assistant

b.Supervisor: Sheli R. Radoshitzky, PhD

c.May 2009-July 2009

d.Fort Detrick, MD

e.Worked in BSL-2 virology laboratory as a research technician performing functions such as western blot, plasmid DNA cloning, and mammalian tissue culture.

Publications

Meghna U. Naik, Pravin Patel, Randall Derstine, Ramya Turaga, Xi Chen, Kalyan, Golla, Keith B. Neeves, Hidenori Ichijo, and Ulhas P. Naik. Ask1 Regulates Platelet Granule Secretion, Thromboxane A2 Generation, and Thrombus Formation in Mice. Blood. 2016. 07-729780.

Awards/Accomplishments

Alpha Lambda Delta, Freshman Honor Society, Outstanding Achievement (2006)

Competitive GPA Grant (2006)

Dean’s List Recipient (2006, 2007, 2008)

Beta Beta Beta Biological Honor Society, Outstanding Achievement in Biology (2008)

Thomas C. Canover Memorial Scholarship (2008)

Rettew Associates Inc. Scholarship (2008)

Nominated as Student Tutor for Organic Chemistry (2008)

Elected Secretary of Beta Beta Beta (2009)

Beta Beta Beta Biological Honor Society Grant (2009)

Internship at United States Army Medical Research Institute of Infectious Diseases (2009)

Benjamin & Hannah Sachs Cantor Scholarship (2010)

Poster Presentation at 43rd Annual Philadelphia Workshop on Hemostasis, Thrombosis, and Atherosclerosis at Temple University (2014)

Departmental Travel Award, University of Delaware Department of Biological Sciences (2014)

Professional Development Award, University of Delaware School of Graduate and Professional Education (2014)

Winner of Outstanding Abstract Achievement Award, American Society of Hematology (2014)

Poster Presentation at 44th Annual Philadelphia Workshop on Hemostasis, Thrombosis, and

Atherosclerosis at University of Pennsylvania (2015)

References

*Available upon request.



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