Assistant Quality Assurance
Resume:
Kristen Marie Sweet
(Maiden name: Kristen Marie Tamburro)
*****************@*****.***
**** **** ******, ************, ** 19103
WORK EXPERIENCE:
Graduate Research Assistant, University of North Carolina, Chapel Hill, NC
2007-2013
Studied AIDS-associated herpesviruses. Developed high-throughput
automated qPCR methods to detect and profile herpesviral infections for
clinical research studies. Modified lab to abide by Good Clinical Lab
Practices (GCLP) including SOP development and Quality Assurance/Quality
Control.
Intern, National Foundation for Celiac Awareness, Ambler, PA
2013
Analyzed data from Celiac Awareness Continuing Medical Education program
participants.
Undergraduate Research Assistant, Lehigh University, Bethlehem, PA
2005-2007
Studied the role of RNA editing using cloning techniques to generate
defective editing enzymes for future studies using a mouse model. Wrote
honors thesis.
Intern, Center for Emeritus Scientists in Academic Research, Bethlehem, PA
2006
Studied polyoxomolybdates macro anions as potential drug delivery
vesicles for summer internship.
RELATED SKILLS:
qPCR and PCR
Automated liquid handling robots
(Tecan Freedom Evo, Agilent m2000, Roche MagNApure- compact and 96)
Robot programing (Tecan EvoWare)
High-throughput experiments
DNA ELISA
Immunohistochemistry
Human specimen processing
(blood, tumors, PAP specimens)
Nucleic acid extraction and purification
Clinical research studies
Translational medicine
HPV strain-typing
Next-gen sequencing
Cloning
Tissue culture
Western blots
Cryostat tissue sectioning
Good Clinical Lab Practices (GCLP)
Microsoft Office
R statistical programming language
EDUCATION:
Doctor of Philosophy, Curriculum in Genetics and Molecular Biology
January 2013
University of North Carolina, Chapel Hill, NC; Advisor- Dirk P. Dittmer,
PhD
Thesis: Profiling for KSHV and Other Herpesviruses in vivo in Clinical
Specimens
Certificate in Translational Medicine
January 2013
University of North Carolina, Chapel Hill, NC; Clinical Co-Mentor:
Charles van der Horst, MD
Bachelor of Science, Biology
May 2007
Lehigh University, Bethlehem, PA
CLINICAL TRIALS EXPERIENCE:
AIDS Malignancy Consortium (AMC) Pilot Study S001: 70 patient international
study.
Investigated the potential use of Ganciclovir as therapeutic drug for
Kaposi Sarcoma in AIDS patients in Malawi. Developed viral gene
expression assay using high-throughput qPCR. Developed assay to determine
viral load from plasma specimens. Performed all experiments for trial,
analyzed data and reported results to collaborators (manuscript in
preparation).
AIDS Clinical Trials Group (ACTG) Study A5254: 360 patient, multi-site,
cross-sectional study.
Examined salivary viral load of herpesviruses. Designed and developed
SOPs to isolate and quantify herpesviruses in saliva using high-
throughput automated qPCR. Contributed to designing Manual of Operations
and participated in study-related conference calls.
UNC AIDS Malignancy Trial: Observational study at UNC Center for Infectious
Disease.
Initiated new observational trial at UNC hospital aimed to obtain
clinical specimens from HIV patients who have a malignancy. Assisted with
IRB development and trial publicity. Stored and processed blood and tumor
specimens, and maintained patient records for blinded samples.
LEADERSHIP AND TEACHING EXPERIENCE:
Research Mentor, University of North Carolina, Chapel Hill, NC
2011
Mentored and collaborated with visiting graduate student from University
of Sydney, Australia. Taught high-throughput qPCR Cytomegalovirus gene
expression array for early detection of CMV infection flares in
transplant recipients (Patent pending- Cytomegalovirus Signature Assay).
Teaching Assistant, Undergraduate Genetics Course, University of North
Carolina, NC 2008
Prepared and led recitations 3 hours/week. Held office hours, prepared
students for exams, graded assignments and exams.
Assistant Aerial Silks Instructor, Triangle Yoga, Chapel Hill, NC
2010
Assisted with beginner aerial silks fitness class. Demonstrated moves for
the purpose of teaching, gave detailed instruction, spotted students, and
answered questions.
Waitress, Tugboats Restaurant, Hyannis, MA
2007
Served customers, prepared dining area, ran the register, and cleaned
before and after service.
Apprentice Teacher, Undergraduate Genetics Course, Lehigh University,
Bethlehem, PA 2006
Held office hours and review sessions before exams as extra help for
students. Assisted students with questions they had pertaining to course
material.
Supervisor, Student Fitness Center, Lehigh University, Bethlehem, PA
2006-2007
Supervised other employees, made schedules, resolved issues, and
maintained paperwork.
Assistant Teacher, AP and Regents Biology, Ward Melville High School,
Setauket, NY 2005
Assisted biology teacher with three high school biology classes. Prepared
laboratory setups, assisted students with labs, prepared exam questions,
graded papers, and assisted in lesson preparation.
Coxswain, Crew Team, Lehigh University, Bethlehem, PA
2003-2004
Crew team motivator and leader, responsible for steering boat and
maintaining team morale.
HONORS AND AWARDS:
Howard Hughes Translational Medicine Training Grant recipient
2009-2011
University of North Carolina, Chapel Hill, NC
Genetics and Molecular Biology T32 Training Grant
2009 University of North Carolina, Chapel Hill, NC
Departmental Honors
2007
Lehigh University, Bethlehem, PA.
PUBLICATIONS:
Tamburro, KM, Yang, D, Poisson, J, Fedoriw, Y, Roy, D, Lucas, A, Sin, SH,
Malouf, N, Moylan, V, Damania, B, Moll, S, Van der Horst, C, Dittmer, DP.
Vironome of Kaposi Sarcoma associated herpesvirus-inflammatory cytokine
syndrome in an AIDS patient reveals co-infection of human herpesvirus 8
and human herpesvirus 6A. Virology, 2012.
Tamburro, K*, Chugh, P*, Dittmer, D. Profiling of pre-micro RNAs and
microRNAs using
quantitative real-time PCR (qPCR) arrays. J Vis Experiments, 2010.
*Authors contributed equally
http://www.jove.com/index/details.stp?id=2210, doi: 10.3791/2210
Yang, D, Tamburro, K, Dittmer, D, Cui, X, McVoy, M, Hernandez, N, Schleiss,
M. "Complete Genome Sequence of Pathogenic Guinea Pig Cytomegalovirus
from Salivary Gland Homogenate of Infected Animals." American Society for
Microbiology, 2013.
Roy, D, Sin, SH, Lucas, A, Venkataramanan, R, Wang, L, Eason, A, Chavakula,
V, Hilton, A, Tamburro, K, Damania, B, Dittmer, D."MTOR inhibitors stall
Kaposi sarcoma growth by inhibiting essential autocrine growth factors
and tumor angiogenesis." Cancer Research, 2013.
Dillon, PJ, Gregory, SM, Tamburro, K, Peck, M, Johnson, G, Raab-Traub, N,
Dittmer, DP,
Damania, B. A Human siRNA Kinome Screen Identifies TLKs as Modulators
of Herpesviral Reactivation from Latency. Cell Host and Microbe, 2013.
Maas, S, Kawahara, Y, Tamburro, KM, and Nishikura, K. A-to-I RNA editing
and human disease.
RNA Biology, 3(1): 1-9, 2006.
SELECTED ORAL PRESENTATIONS (for complete list, please contact me):
Tamburro, KM (2013). "Thesis Defense: Profiling for KSHV and Other
Herpesviruses In Vivo In Clinical Specimens." UNC, Chapel Hill, NC.
Tamburro, KM (2011). "Kaposi Sarcoma gene expression profiles in patients
in Malawi." Virology In Progress Seminar, UNC, Chapel Hill, NC.
Tamburro, KM (2010). "AMC S001: A Pilot Study of KSHV Gene Expression in
Patients with Newly Diagnosed Kaposi Sarcoma (KS) in Malawi, Africa."
AIDS Malignancy Consortium (AMC) Investigator's Meeting, Bethesda, MD.
Tamburro, KM (2010). "Clinical Trials: From the Perspective of a Research
Laboratory." AIDS Malignancy
Consortium (AMC) Investigator's Meeting, Bethesda, MD.
Tamburro, KM (2010). "Viral Load of Herpesviruses: Moving a Lab Assay to
GCLP." Genetics Student Seminar, UNC, Chapel Hill, NC.
Tamburro, KM (2009). "Forensic Virology of HIV-Associated Mortality."
Translational Medicine Closed Door Seminar, UNC, Chapel Hill, NC.
Contact this candidate