Project Assistant

Xin GAO

The Genome Institute,

Email: ****@******.*****.***

Washington University School of Medicine

Phone: 515-***-**** (mobile)

**** ****** **** ***, **. Louis, MO 63108

EDUCATION

The George Washington University, Washington, DC

Ph.D., Microbiology 2007 - 2011

Dissertation Title: Characterization of the Hookworm FOXO Transcription

Factor DAF-16 and its Target Genes

M.S., Pathogenic Biology Chinese Center for Disease Control and Prevention (China CDC), Beijing, China 1996 - 1999

Thesis Title: A Preliminary Investigation of Gene Expression in Adult

Schistosome japonicum

Fudan University, Shanghai, China

B.S., Biochemistry 1991 - 1996

SKILL BASE

Computational biology: Next-generation Sequencing Data Processing and Analysis Microarray Data Analysis

Proteomics Data Analysis Functional and Comparative Genomics

Scripting Languages (Perl, SHELL) Statistic Packages (R, R-based Bioconductor)

Computer Skills:

Operating System (Linux/Unix, Windows, Mac) LSF Platform Microsoft Office

Applications Adobe Photoshop Internet-based and Stand Alone Biological Sciences

Applications SigmaPlot

Mammalian Cell Culture Cell Transfection

Cell Biology:

PCR Real-time PCR DNA subcloning RACE Gene Expression and Protein

Molecular Biology:

Purification Western Blot DNA and RNA Isolation cDNA synthesis Site-directed

Mutagenesis

HPLC Ion Exchange Chromatography Affinity Chromatography Enzymatic Assays

Biochemistry:

EMSA Protein Crystallography Pull Down Assay Gel Electrophoresis of Nucleotide

and Protein

ELISA Immunoprecipitation Immunohistochemistry

Immunology:

C. elegans Strains Maintenance and Manipulation RNAi Feeding Assay Parasitic

Worm Techniques:

Hookworm Larvae Culture and Maintenance Hookworm Activation Assay Adult

Hookworm Recovery Parasitic Brugia malayi Adult Worm Manipulation

Language Skills: Fluent in Chinese (Mandarin) and English

RESEARCH EXPERIENCE

Staff Scientist, The Genome Institute, Washington University School of Medicine, St. Louis, MO, 2012 – present

Research Project: Genomics, Transcriptomics, and Proteomics of parasitic nematode

Postdoc Research Associate, The Genome Institute, Washington University School of Medicine, St. Louis, MO,

2011 – 2012

Research Project: Genomics, Transcriptomics, and Proteomics of parasitic nematode

Research Associate (part-time), Dept. of Microbiology and Tropical Medicine, The George Washington University

Medical Center, Washington DC, 2007 – 2011

Research Project: Biochemistry and molecular biology of Insulin-like Signaling pathways in the infective larvae

of hookworms

Graduate Research Assistant, Dept. of Biochemistry, Biophysics and Molecular Biology, Iowa State University,

Ames, IA 2002 – 2006

Research Project: Structure and function of Plasmodium adenylosuccinate synthetase (ADSS), porcine fructose

bisphosphatase (FBPase), and porcine phosphoribosylaminoimidazolesuccinocarboxamide

synthetase (SAICAR synthetase)

Graduate Research Assistant, Dept. of Biological Sciences, Louisiana State University, Baton Rouge, LA, 2000 –

2001

Research Project: Activity and structure analysis of plant chitinases.

Research Associate, Chinese National Human Genome Center at Shanghai, Shanghai, China, 1999 – 2000

Research Project: Gene expression profile of liver cancer, EST method

Graduate Research Assistant, Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention

(China CDC), Shanghai, China, 1996 – 1999

Research Project: Expressed sequence tags (EST) in Schitosoma japonicum Genome – Collaborative project with

Queensland Institute of Medical Research (QIMR), Brisbane, Australia

TEACHING EXPERIENCE

Graduate Teaching Assistant, Dept. of Biochemistry, Biophysics and Molecular Biology, Iowa State University,

Ames, IA, 8/2002 – 12/2002

Subject: BBMB 411 General Biochemical Research Techniques

Supervised Students at The George Washington University, 2007 – 2011

undergraduate student 2007 – 2008

Josh Kiss

Emily Nelson undergraduate student summer 2008

undergraduate student 2008 – 2009

Jason Qian

Danielle Skinner Ph.D. rotation student spring 2009

undergraduate student 2010 – 2011

Kevin Goggin

PUBLICATIONS

Wang, Z.*, Gao, X.*, John Martin, J., Yin, Y., et al. (2013) Gene expression analysis distinguishes tissue specific and

gender related functions among adult Ascaris suum tissues. Molecular Genetics & Genomics, accepted. (* co-first

authors)

Heizer, E., Zarlenga, D.S., Rosa, B., Gao, X., et al. (2013) Transcriptome analyses reveal protein and domain families

that delineate stage-related development in the economically important parasitic nematodes, Ostertagia ostertagi

and Cooperia oncophora. BMC Genomics. 14(1):118.

Gelmedin, V., Brodigan, T., Gao, X., Krause, M., et al. (2011) Transgenic C. elegans Dauer Larvae Expressing

Hookworm Phopho Null DAF-16/FoxO Exit Dauer. Plos One. 6(10): e25996

Gao, X., Wang, Z., Martin, J., Abubuker, S., et al. (2010) Identification of Hookworm DAF-16/FOXO Response

Elements and Direct Gene Targets. PLoS One. 5 (8), e12289

Wang, Z., Zhou, X.E., Motola, D.L., Gao, X., et al. (2009) Identification of the nuclear receptor DAF-12 as a therapeutic

target in parasitic nematodes. Proc Natl Acad Sci U S A. 106 (23), 9138-43

Kiss, J.E., Gao, X., Krepp, J.M., Hawdon, J.M.. (2009) Interaction of hookworm 14-3-3 with the forkhead transcription

factor DAF-16 requires intact Akt phosphorylation sites. Parasites & Vectors. 2 (1), 21

Gao, X., Frank, D., Hawdon J.M..(2009) Molecular cloning and DNA binding characterization of DAF-16 orthologs

from Ancylostoma hookworms. International Journal for Parasitology. 39 (4), 407-15

Li, H., Zhang, Y. H., Gao, X., Sun, J., et al. (2002). The construction of monoclonal antibody cell line against

Schistosoma japonicum recombinant protein 20.8 kDa antigen. Chinese Journal of Parasitology and Parasitic

Diseases. 20 (1), 34 (in Chinese)

Gao, X., Feng, Z.. (2000) Progress in the research on Schistosome genome. Chinese Journal of Parasitology and Parasitic

Diseases. 18 (1), 52-55 ( in Chinese, invited review)

Blackwell, J.M., Gao, X., Wu, B.. (1998) Progress in the Leishmania genome project. Foreign Medical Sciences –

Parasitic Diseases. 25 (3), 116-118 (in Chinese, invited academic article translation)

MANUSCRIPTS

Tang, Y.*, Gao, X.*, Rosa, B.*, Abubucker, S., et al. Genome of the human hookworm Necator americanus.

(submitted, * co-first authors)

Gao, X., Goggin, K., Dowling, C., Qian, J., Hawdon J.M., et al. Hookworm small nuclear ribonucleoprotein-3: gene

structure, expression profile, and implications of its 3 -UTR in the regulation of gene expression. (in preparation)

CONFERENCE PRESENTATIONS

Gao, X., Abubucker, S., Rosa, B., Jasmer, D.P., Mitreva, M. (2012) Nematode Intestine and Its Impact on

Parasite Control Discoveries. The American Society of Tropical Medicine and Hygiene 61st Annual Meeting,

Atlanta, GA, U.S.A., poster

Gao, X., Abubucker, S., Jasmerm D., Mitreva, M.. (2012) Preliminary Characterization of the Nematode Intestinal

Transcripome. 2012 EEID PI meeting, Berkeley, CA, U.S.A., Poster

Gao, X., Hawdon, J.M.. (2010) Expression Pattern of Hookworm DAF-16/FOXO Targets During Early Parasitic

Development. The American Society of Tropical Medicine and Hygiene 59th Annual Meeting, Atlanta, GA, U.S.A.,

poster

Gao, X., Wang, Z., et al. (2010) Identification of Hookworm DAF-16/FOXO Response Elements and Direct Gene

Targets. C. elegans Topics Meeting on Aging, Stress, Pathogenesis, and Small RNAs, Madison, WI, U.S.A. poster

Gao, X., Hawdon, J.M.. (2009) Identifying the Binding Elements and Target Genes of Hookworm DAF-16. American

Society of Parasitologists, annual conference, Knoxville,Tennessee, U.S.A., oral

Gao, X., Hawdon, J.M.. (2008) Molecular Investigation of Ancylostoma caninum DAF-16. American Society of

Parasitologists Arlington, annual conference, Texas, U.S.A., oral

Gao, X., Hawdon, J.M.. (2007) Ancylostoma caninum DAF-16 Binds to a Conserved DNA sequence. Helminthological

Society of Washington. Washington, D.C., U.S.A., oral

Gao, X., Feng, Z.. (1998) Research Proceedings of Schistosome Genome. National Conference of the Medical

Parasitology, Guangzhou, China, oral (Chinese)

Gao, X.. (1998) EST in Schistosoma japonicum genome. Report Meeting of the Key Laboratory of the Ministry of Public

Health, Shanghai, China, oral (Chinese)

PROFESSIONAL ACTIVITIES

Manuscript Reviewer for

Parasitology

Title of article: Aspartic protease Sc-ASP110 gene transcript in Steinernema carpocapsae and their molecular analysis

Journal of Parasitology

Title of article: Gene Cloning And Characterization Of The Protein Encoded By The Neospora Caninum

Bradyzoite-Specific Antigen Gene Bag1

Parasites &Vectors

Title of article: Duplex quantitative real-time PCR assay for the detection and discrimination of the eggs of Toxocara

canis and Toxocara cati (Nematoda, Ascaridoidea) in fecal samples

Comparative Parasitology

Title of article: In vitro effects of extracts of the barks of Schumanniophyton magnificum, Pseudospondias microcarpa

and Rauvolfia vomitoria, on free larval stages of Heligmosomoides bakeri (Nematoda,

Heligmosomatidae)

BBA - Molecular Cell Research

Title of article: Role of GALNT2 in the modulation of ENPP1 expression, insulin signaling and action

Journal of Asthma and Allergy

Title of article: Racial Difference in the Association of CD14 Polymorphisms with Serum Total IgE Levels and

Allergen Skin Test Reactivity

AWARDS AND HONORS

NIH National Graduate Student Research Festival Travel Grant, National Institutes of Health, 2010 (200 awardees

selected from 602 nationwide applicants in the U.S.)

Travel Award Recipient, American Society of Parasitologists, 2009 & 2008

Student Presentation Competition Winner, Helminthological Society of Washington, 2007

The Nominee for the WHO Informatics Workshop in Cambridge, UK, 1999

UNDP/World Bank/WHO-TDR scholarship, 1999

China-Australia Cooperative Project scholarship, 1998

Honored Graduate Student, Chinese Center for Disease Control and Prevention (China CDC), Beijing, China, 1997

Renmin Scholarship, Fudan University, Shanghai, China, 1996 & 1997

PROFESSIONAL MEMBERSHIPS

2008 – 2011

American Society of Parasitologists

2007 – 2011

Helminthological Society of Washington

PATENTS

Co-inventor of 13 patents in human gene discovery (China Patent):

CN1277999, 2000; CN1277998, 2000; CN1277997, 2000; CN1272541, 2000; CN1272540, 2000;

CN1272539, 2000; CN1270222, 2000; CN1270221, 2000; CN1270220, 2000; CN1269418, 2000;

CN1269417, 2000; CN1269409, 2000; CN1269408, 2000;

VOLUNTEER ACTIVITIES

Cultural Consultant, Maridian International Center, Washington DC, 2007-2008

Volunteer for Science Olympiad National Tournament, The George Washington University, Washington DC,

2008

Consultant, The BALSA Group, St. Louis MO, 2012

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