Manufacturing Technician

MONISHA RAVICHANDRAN

**** ******** ******, *******, **-94536

Phone- 213-***-****, *******@*****.***

EDUCATION

University of Southern California (USC), Los Angeles, CA

(August 2012)

Master of Science, Biochemistry and Molecular Biology, CGPA 3.33/4.0

Anna University (AU), Chennai, India

(May 2010)

Bachelor of Technology, Biotechnology

WORK EXPERIENCE

Life Technologies, Custom Primer and Oligonucleotide Manufacturing

Department, Pleasanton, CA

Manufacturing Technician

(January 2013-Present)

Synthesis of custom Primers/Oligonucleotides following a SOP, time

management, troubleshooting test results and instruments, 6S, GDP and

maintaining batch records.

University of Southern California, Department of Biochemistry & Molecular

Biology, CA

Master of Science Thesis work (August

2011- August 2012)

"Alcohol mediated expression of cyto-protective enzyme- NQO-1 and its post

translational regulation"

Techniques used: Mammalian cell culture, RNA isolation by Trizol-Chloroform

method, Primer 3, qRT-PCR, cDNA synthesis, putative miRNA identification by

Microcosm Targets, miRNA isolation, detection by qRT-PCR and quantification

using Nanodrop, DNA isolation and quantification, Transformation,

Transfection, Protein quantification assays, Luciferase assays, SDS-PAGE

and Western Blotting.

Rinat Neuroscience Corporation (Pfizer Inc.), Antibody Engineering

Department, CA

Research & Development Intern

(June- August 2011)

"Evaluation and identification of the phage constructs that has the

capability to display bi/multivalent antibodies without any structural

or functional deformation to the antibody"

Techniques used: Phage display of antibodies using M-13 phage, Phage

purification, Phage panning, Phage titration, Phage ELISA, SoftmaxPro,

Western Blotting.

Rajiv Gandhi Center for Biotechnology, Utero Embryo Repromics

laboratory, India

Research & Development Intern

(June-July 2009)

"Identification of role of AIRE gene in human blood for prediction of

PCOS"

Techniques used: Genomic DNA isolation from human blood using Phenol-

Chloroform method and QIAGEN kit method, DNA quantification by UV

spectrophotometer, Agarose gel electrophoresis, PCR.

RELEVANT COURSEWORK AT USC

Biochemistry, Predictive & Prognostic Biomarkers in Cancer, Infection &

Host responses, Molecular Genetics, Cell Biology, Viral Oncology,

Introduction to Pathology, Systematic Research writing

LABORATORY SKILLS

Molecular Biology: DNA- isolation & quantification, PCR, RNA isolation &

quantification, qRT- PCR, miRNA- isolation, detection & quantification,

cDNA synthesis, Agarose gel Electrophoresis, Restriction enzyme digestion,

Ligation, Cloning, Transformation, Transfection, ELISA, Western Blotting.

Cell Culture & Assays: Mammalian cell culture, Bacterial Cell culture,

Luciferase assay, Fluorescent Microscopy.

Protein Sciences: Phage Display, Native PAGE, SDS PAGE, Acetone

precipitation, Ultrafiltration, Gel filtration Chromatography, HPLC, AKTA

Prime FPLC, IMAC, TLC, Isotopic labeling of protein, Protein expression &

purification in bacteria & M-13 phage, Protein expression test, Protein

quantification assays.

Immunology: Immuno-diffusion, ELISA, Agglutination and Precipitation.

Microbiology: Culturing & aseptic techniques, Enumerating Bacteria & Phage,

Gram Staining, Microscopic examination.

Bioinformatic Tools & Softwares: BLAST, NEB cutter, Primer3, MicroCosm

Targets, VMD, Pymol, SoftmaxPro.

ACADEMIC PROJECTS

"Isolation and purification of Integrin ?IIb and Integrin ?3 subunits for

analysis of the tilt angle of the subunits with various lipid bilayer

concentrations"

University of Southern California, Los Angeles, California

(September 2010 - March 2011)

Techniques used: Bacterial cell culture, Isotopic labeling of protein,

Protein estimation, Protein expression test, VMD, Pymol, AKTA Prime FPLC,

IMAC, HPLC.

"Studies on Distribution, culturing and genomic DNA isolation from

Tardigrades"

Anna University (Kumaraguru College of Technology), India.

(December 2009- April 2010)

Techniques used: DNA isolation, quantification using UV spectrophotometer,

Agarose gel electrophoresis.

"Isolation and partial purification of industrially important enzymes from

the Spent mushroom compost of Pleurotus florida"

Anna University (Kumaraguru College of Technology), India.

(July 2008-April 2009)

Techniques used: Acetone precipitation, Ultrafiltration, Protein

quantification assays, SDS PAGE, Native PAGE.

COMPUTER SKILLS

MS Office Suite (MS word, Excel & PowerPoint), Programming language C

Honors & Activities

Board member, Association of Indian Students, University of Southern

California (January-August 2011)

Member/Volunteer, Nature club of Kumaraguru College of Technology, India

(September 2009- May 2010)

POSTERS & CONFERENCES

Organized "Genespire'09", a National level technical symposium, Kumaraguru

College of Technology, India

(September 2009)

Presented a poster on "Isolation and partial purification of Industrially

important enzymes from the spent mushroom compost of Pleurotus florida",

Bioconcurrenza'09, TNAU, India (November 2009)

Participated in the 77th Annual Meeting of Society of Biological chemists,

"Impact of Basic and Translational Research on Medicine, Agriculture &

Industry", IIT-Madras, India. (December 2008)

Organized "Genespire'07", a national level technical symposium, Kumaraguru

college of Technology, India.

(September 2007)

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