Sr.Technician Experience Quality Control Engineer
Resume:
FRANK (FEIYUE) LI
(US Citizen)
Germantown, MD 20874
Phone: 301-***-****
Email: ********@*****.***
Summary
Over 20 years of extensive experience in biochemistry and chemistry techniques. Have lead teams of technicians
and works well with teammates. A self-motivated, hard working, master new skills quickly, and have good
communication skills person. Have computer skills in database and Microsoft Word, Excel and corporate
intranets.
Skills and Expertise
Expert in biochemistry and chemistry Experience in manufacturing processing in
GMP/GLP environment ensured production
techniques, experiences at protein/DNA,
timing and quality
RNA, antibody analysis as well as QC
Experience in managing production flow
Strong skills with analysis methods ( HPLC, with excellent problem shooting skills
MS, CE, FPLC, GC/FID, AAS, AFS etc)
Technical documentation / presentation
especially in the chromatography field
writing and update procedures
Extensive working with DNA/RNA, protein,
manufacturing, synthesis, and purification 3 years of supervising and mentoring
experience
Professional Practice
• Protein, antibody extraction, purification, and analysis by IEX, HIC, SEC column, with AKTA Purifier 10,
GE Cross-Flow Filtration, Agilent Affinity LC as well as QC operation with MS, CE
• DNA/RNA products assay using HPLC Agilent 1100, Waters HPLC, PAGE,COP Cartridge,, UV/VIS
spectroscopy
• Automated PE/ABI 377 sequencer for human DNA sequences detection
Western Blot, Genotyping, PCR-Taqman, Cell culture and bacterial fermentation
•
Experience
Life Technologies (formerly Invitrogen) Frederick, MD
Sr. Manufacturing Technician Mar ’02 – Dec ‘11
• Worked in the molecular biology lab under the GMP environment to perform QC sample analysis with MS
(MALDI-TOF, Triple Quads) and CE (Combisep).
• Responsible in the team to purify RNA, DNA/protein including large and small scale by using HPLC Waters
2695, Agilent LC, PAGE, and COP Cartridge methods. As the main force of RNA purification, ensured the all
RNA purification timing and quality. DNA/RNA 96-well plate based quantity assay. Distinguished the results
of mass spectrometry and CE. Dealt with all paper work and database to ensure the on-time delivery of
products. Especially kept high passed rate for the team quarterly goal.
• As a magnetic protein, purified Dynabeads products with SEC column, and pre-coupled it with a biomolecule
such as an antibody, protein or antigen, DNA/RNA probe etc.
• Responsible in the team to synthesize DNA and RNA primers. Operated Jurassic Synthesizer, Oligo Pilot,
ABI 3900 and Mer made12 to produce various DNA, RNA sequences. Some of them were called
semiconductor Sequencing that would be need more carefully. Modified or labeled oligos with special
molecules and dyes (Phosphate, Biotin, HEX, TET, Alexa488 etc.).
• Standardized and reviewed laboratory documentation to help the company that gained ISO 9001:2008
certifications.
• Improved on the procedure of RNA purification. As a result, the RNA purification cost was reduced 30% and
accomplished in 2 hours less time.
• GMP production documentation, including rewrote and devised SOPs & batch records.
• Trained the new group of people to help the company when it relocated production to CA.
.
FMC Corporation Baltimore, MD
QC Lab Technician Nov ’00 – Mar ‘02
• Performed HPLC, Orbitrap LC/MS, and AAS to determine intermediates of insecticides and final products at
the Quality Control Lab. Focused on resolutely implemented SOP to ensure high-quality results to be
achieved rapidly.
• Environmental monitoring that included water and soil sample testing.
• Calculated analytical results and transferred to data base.
• Maintained and prepared the analytical equipments weekly.
Georgetown University / Navy Medical Research Center – Biomedical Research Rockville, MD
Lab Technician Apr ’00 – Oct ‘00
• Performed SEC column and Affinity HPLC for monoclonal antibody analysis from human serum, including AFM1
identification.
• Responsible genotyping for HLA-DR, extract DNA from human blood cells.
• Performed full length genes PCR-Taqman, genotyping and PAGE, autoradiography. Hybridized dot blots with
nonradioactive detected labeled (antibody-Lumiphos complex) probes and ELISA for antibody assay.
• Experienced in using automated PE/ABI 377 sequencer for marrow DNA sequencing analysis and registry.
Food Research Institute /Central Lab Tianjin, China
Laboratory Engineer/ supervisor Apr ’84 – May ‘98
• Gained proficiency in using various analysis machines such as HPLC, UV-visible Absorption, Atomic
Absorption, Atomic Fluorescence Spectroscopy, IR, GC/MS, Scanning Electron Microscopy.
Developed the new type of beer yeast with suspension cell culture and bacterial fermentation methods.
•
• Extraction and analyzing protein with Affinity/LC, PAGE.
• Environmental monitoring that included water and soil sample testing.
• 3 years of supervising and food chemistry analysis mentoring experience.
• Written laboratory SOPs and working flow procedure to gain ISO9000 international certification.
• Devised valid graphite oven to improve absorbance stability in AAS machine.
• New type of application of science in food industry and commerce technique development.
Education
B.S. degree in Analytical Chemistry, Tianjin University, Tianjin, China (1983)
Notable Projects
• Participated in the setting of China’s National Standard for the determination of zinc contents in food using
AFS analysis.
• Improved on the protocol of RNA purification to reduce production cost.
Honors and Awards
• Recognition for dedication in training new employees 2011
• Excellence Award for zero error ratio 2005-2007
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