Plant Project

Dr. SALONI SHARMA

B.Sc.(Biology), M.Sc.(Microbiology), Ph.D. (Microbial Biotechnology)

*********@*****.***

Mobile : +1-408-***-****

Sunnyvale, CA

PROFESSIONAL EDUCATIONAL QUALIFICATIONS

Doctor of Philosophy (Ph.D.) in Microbiology

University : Jai Narain Vyas University, Jodhpur (Rajasthan), INDIA

(Accredited A grade by NAAC, INDIA )

Web Presence: http://www.jnvu.edu.in

Year of Completion : August, 2009

THESIS TOPIC:

"An integrated approach for increasing biomass production, nutrient

uptake and productivity of some medicinal plants of Indian Thar desert."

Supervisor: Dr. Anil Vyas

Master of Science (M.Sc.) in Microbiology

University : University of Rajasthan, Jaipur (Rajasthan), INDIA

(Accredited A+ grade by NAAC, INDIA )

Web Presence: http://www.uniraj.ernet.in

Aggregate : 64 % (GPA: 3.01 out of 4)

Year of Completion : 2005

Bachelor of Science (B.Sc.) in Biology (Botany, Chemistry, Zoology)

University : Jai Narain Vyas University, Jodhpur (Rajasthan), INDIA

(Accredited A grade by NAAC, INDIA )

Web Presence: http://www.jnvu.edu.in

Aggregate : 63 % (GPA: 3.01 out of 4)

Year of Completion : 2002

Schooling

Exam Year Percentage

Sr. Secondary(with Biology) 1999 68 % (GPA: 3.20/4) Rajasthan Board

(12th)

Secondary (10th) 1996 73 % (GPA: 3.44/4) Rajasthan Board

TECHNICAL KNOW-HOW

Skills Bacteriology, Virology, Immunology, Biotechnology,

Environmental sciences, Microbial technology,

Biochemistry, Molecular biology, Environmental

microbiology, food microbiology, cellular microbiology,

medical microbiology, recombinant DNA technology,

Bioinformatics, Mycology, Phycology.

Computer Office tools and basic computer knowledge

skills

MANUSCRIPTS IN CONFERENCES AND PAPERS

. Paper presented on "Mycorzhizal association and Biomass Production of

Some Medicinal Plants" at Dr. Tanveer Malawat College of Bioscience,

Bikaner (Rajasthan), INDIA.

. Poster presentation in National Conference on new vistas in

microbiology and plant sciences and awarded for second prize at

Haridwar.

. Conference attended on "National Symposium on Biotechnology for human

Welfare (The Commercial and Ethical Consideration)" at Rajasthan

University (Botany Department), Jaipur (Rajasthan) INDIA.

. Conference attended on "National Conference on Emerging Trends in

Microbiology and biotechnology" at Dr. Tanveer Malawat College of

Bioscience, Bikaner (Rajasthan) INDIA.

. International Conference attended on "International symposium on

organic farming and renewable sources of energy for sustainable

agriculture" at Udaipur, India.

. Workshop attended on "Bioinformatics/computer application in life

sciences" from university science instrumentation centre, Jai Narain

Vyas University, Jodhpur, Rajasthan, India.

TEACHING EXPERIANCE

1) 2006-2008 at Jai Narain Vyas University, Jodhpur (Raj) INDIA.

. 2 Years Teaching Experience from year 2006 - 2008

. Position: Guest faculty

. University: Jai Narain Vyas University (Accredited A grade by NAAC),

New Campus,

Jodhpur (Rajasthan), INDIA

. Department: Department of Botany

. Courses: M.Sc. Microbiology, B.Sc. Microbiology

HANDS ON EXPERIENCE

1) "Dissertation Description "

Objectives:

. Collection and identification of AM fungi associated with medicinal

plants from Indian Thar Desert.

. To culture the AM isolates on some suitable hosts like cenchrus

ciliaris for mass multiplication and inoculums production

. To select the efficient AM strain for medicinal plants for its biomass

production and productivity.

. To evaluate influence of AM endophytes and various plants growth

promoting microorganism on various physiological and biochemical

changes in medicinal plants.

. To apply selected efficient AM strain and plant growth promoting

microorganisms for increasing biomass production of the medicinal

plants.

Research Methodology:

. Intensive periodical survey of the various regions of arid and semi

arid areas of Indian Thar Desert will be undertaken to collect and

describe AM endophytes associated with medicinal plants.

AM Endophytes:

. Root and soil samples was collected to recover AM fungal spores using

wet sieving and decanting technique of Gerdmann and Nicolson (1963)

. Anatomy of infected roots was described after suitable staining

(Phillips and Hayman).

. The percentage of root colonization calculated by grid line intersect

method (Giovannetti and Mosse, 1980), followed to isolate the local AM

fungal associates and establish them in pot culture.

Biochemical Analysis

. Peroxidase activity in the plant material was measured by

incubating the enzyme with guaiacol & hydrogen peroxide (Racusen &

Foote, 1965).

. Polyphenol oxidase activity will be measured at 420 nm, using the

method of mahadevan (1975).

Physiological Studies

. Soluble proteins in plant material determine by the comassive blue

method (Bradford, 1976).

. Total phenols in the plant material determine by using folin

ciocalteu's reagent (Bray and Thrope, 1954).

. Chlorophyll estimating of the plant material done by the method of

Lichenthaler & Wellburn (1983).

. Various nutrient viz phosphorus, nitrogen, etc estimate in the

plant material by the method of Jackson (1973).

. Total sugar and starch in the plant materials estimated by the

method of Dubius et al (1965).

2) "Microbiological Analysis of Different Pharmaceutical Samples"

Description: I have done my M.Sc project training at RANBAXY LABORATORY Ltd

(R&D), Gurgaon- Haryana, INDIA for full time 2 months.

Web Presence: http://www.ranbaxy.com

3) "Immunological Testing"

Description: I have done my short term project training at MAHATMA GANDHI

HOSPITAL, Jodhpur- Rajasthan, INDIA for full time 1 month.

The following tests had done during my work in Lab:

. Gram staining And Antibiotic susceptibility test

. Immunoelectrophoresis And ELISA

. Widal test And Total leucocyte count

. Octerlony double diffusion And Differential leucocyte count

4) "Microbiological Testing"

Description: I have done my short term project training at SARAS DAIRY,

Jodhpur for full time 1 month.

The following tests have been done during my work in Lab:

. Testing of Microbes which are present in Milk.

. Methylene blue reductase test

. Pasteurization

. Determination of number of bacteria in milk

. Detection of coliform bacteria in pasteurized milk sample

. Estimation of lactic acid in milk & curd

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